FC930632

Overview
NameFC930632
Unique NameFC930632
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length718
Libraries
Library NameType
PostHarvC1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig900 contig Ccv1_Contig900:2..720. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_PANTR (Triosephosphate isomerase OS=Pan troglodytes GN=TPI1 PE=2 SV=2)

HSP 1 Score: 198.749 bits (504), Expect = 2.307e-52
Identity = 100/194 (51.55%), Postives = 127/194 (65.46%), Query Frame = 2 
Query:   77 ARKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            +RKFFVGGNWK NG  + + +++ TLN A+                     K  L     VAAQNC+    GAFTGE+S  M+ +    WV+LGHSERR +  ES++ +G KVA+AL+ GL VIAC+GE L++RE+G T  VV EQTK IA+ + +W  VVLAYEPVWAIGTGK ATP QAQEVH +LR WLK+
Sbjct:    4 SRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQK--LDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKS 195

HSP 2 Score: 28.4906 bits (62), Expect = 2.307e-52
Identity = 14/20 (70.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            NVS  VA STRIIYGG   G
Sbjct:  196 NVSDAVAQSTRIIYGGSVTG 215
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_HUMAN (Triosephosphate isomerase OS=Homo sapiens GN=TPI1 PE=1 SV=2)

HSP 1 Score: 198.749 bits (504), Expect = 2.307e-52
Identity = 100/194 (51.55%), Postives = 127/194 (65.46%), Query Frame = 2 
Query:   77 ARKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            +RKFFVGGNWK NG  + + +++ TLN A+                     K  L     VAAQNC+    GAFTGE+S  M+ +    WV+LGHSERR +  ES++ +G KVA+AL+ GL VIAC+GE L++RE+G T  VV EQTK IA+ + +W  VVLAYEPVWAIGTGK ATP QAQEVH +LR WLK+
Sbjct:    4 SRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQK--LDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKS 195

HSP 2 Score: 28.4906 bits (62), Expect = 2.307e-52
Identity = 14/20 (70.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            NVS  VA STRIIYGG   G
Sbjct:  196 NVSDAVAQSTRIIYGGSVTG 215
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_CHICK (Triosephosphate isomerase OS=Gallus gallus GN=TPI1 PE=1 SV=2)

HSP 1 Score: 200.675 bits (509), Expect = 2.307e-52
Identity = 102/193 (52.85%), Postives = 129/193 (66.84%), Query Frame = 2 
Query:   80 RKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            RKFFVGGNWK NG  + + +++ TLN A+                     K  L +   VAAQNC+    GAFTGE+S  M+ ++   WVILGHSERR +  ES++ +G KVA+AL+ GL VIAC+GE L++RE+G T  VV EQTKAIA+ + +W  VVLAYEPVWAIGTGK ATP QAQEVH +LR WLK+
Sbjct:    4 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSADTEVVCGAPSIYLDFARQK--LDAKIGVAAQNCYKVPKGAFTGEISPAMIKDIGAAWVILGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKAIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKS 194

HSP 2 Score: 26.5646 bits (57), Expect = 2.307e-52
Identity = 13/20 (65.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            +VS  VA STRIIYGG   G
Sbjct:  195 HVSDAVAQSTRIIYGGSVTG 214
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_BOVIN (Triosephosphate isomerase OS=Bos taurus GN=TPI1 PE=2 SV=3)

HSP 1 Score: 200.29 bits (508), Expect = 3.007e-52
Identity = 101/194 (52.06%), Postives = 128/194 (65.98%), Query Frame = 2 
Query:   77 ARKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            +RKFFVGGNWK NG    + ++++TLN A+                     K  L     VAAQNC+    GAFTGE+S  M+ +L   WV+LGHSERR +  ES++ +G KVA+AL+ GL VIAC+GE L++RE+G T  VV EQTK IA+ + +W  VVLAYEPVWAIGTGK ATP QAQEVH +LR WLK+
Sbjct:    4 SRKFFVGGNWKMNGRKNNLGELINTLNAAKVPADTEVVCAPPTAYIDFARQK--LDPKIAVAAQNCYKVANGAFTGEISPGMIKDLGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKS 195

HSP 2 Score: 26.5646 bits (57), Expect = 3.007e-52
Identity = 13/20 (65.00%), Postives = 13/20 (65.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            NVS  VA S RIIYGG   G
Sbjct:  196 NVSDAVAQSARIIYGGSVTG 215
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_PIG (Triosephosphate isomerase OS=Sus scrofa GN=TPI1 PE=2 SV=4)

HSP 1 Score: 201.83 bits (512), Expect = 3.921e-52
Identity = 102/194 (52.58%), Postives = 128/194 (65.98%), Query Frame = 2 
Query:   77 ARKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            ARKFFVGGNWK NG    + ++++TLN A+                     K  L     VAAQNC+    GAFTGE+   M+ +L   WV+LGHSERR +  ES++ +G KVA+AL+ GL VIAC+GE L++RE+G T  VV EQTK IA+ + +W+ VVLAYEPVWAIGTGK ATP QAQEVH +LR WLKT
Sbjct:    4 ARKFFVGGNWKMNGRKNNLGELINTLNAAKLPADTEVVCAPPTAYIDFARQK--LDPKIAVAAQNCYKVANGAFTGEIGPGMIKDLGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWNKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKT 195

HSP 2 Score: 24.6386 bits (52), Expect = 3.921e-52
Identity = 12/20 (60.00%), Postives = 13/20 (65.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            +V   VA STRIIYGG   G
Sbjct:  196 HVPEAVAHSTRIIYGGSVTG 215
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPISB_DANRE (Triosephosphate isomerase B OS=Danio rerio GN=tpi1b PE=2 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 3.921e-52
Identity = 100/193 (51.81%), Postives = 129/193 (66.84%), Query Frame = 2 
Query:   80 RKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            RKFFVGGNWK NG  + ++++ +TLN A+                     +S L  +  VAAQNC+    GAFTGE+S  M+ +  + WVILGHSERR +  ES++ +G KVA+AL  GL VIAC+GE L++RE+G T  VV  QTK IA+ + +W  VVLAYEPVWAIGTGK A+P QAQEVH +LR+WLKT
Sbjct:    4 RKFFVGGNWKMNGDKKSIEELANTLNSAKLNPDTEVVCGAPTIYLDYA--RSKLDPNIDVAAQNCYKVAKGAFTGEISPAMIKDCGVKWVILGHSERRHVFGESDELIGQKVAHALENGLGVIACIGEKLDEREAGITEKVVFAQTKFIADNVKDWSKVVLAYEPVWAIGTGKTASPQQAQEVHDKLRQWLKT 194

HSP 2 Score: 26.5646 bits (57), Expect = 3.921e-52
Identity = 13/20 (65.00%), Postives = 13/20 (65.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            NVS  VA S RIIYGG   G
Sbjct:  195 NVSEAVANSVRIIYGGSVTG 214
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_GORGO (Triosephosphate isomerase OS=Gorilla gorilla gorilla GN=TPI1 PE=3 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 6.662e-52
Identity = 99/194 (51.03%), Postives = 126/194 (64.95%), Query Frame = 2 
Query:   77 ARKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            +RKFFVGGNWK NG  + + +++ TLN A+                     K  L     VAAQNC+    GAFTGE+S  M+ +    WV+LGHSERR +  ES++ +G KVA+AL+ GL VIAC+GE L++RE+G T  VV EQTK I + + +W  VVLAYEPVWAIGTGK ATP QAQEVH +LR WLK+
Sbjct:    4 SRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTKVVCAPPTAYIDFARQK--LDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVITDNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKS 195

HSP 2 Score: 28.4906 bits (62), Expect = 6.662e-52
Identity = 14/20 (70.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            NVS  VA STRIIYGG   G
Sbjct:  196 NVSDAVAQSTRIIYGGSVTG 215
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_XENLA (Triosephosphate isomerase OS=Xenopus laevis GN=tpi1 PE=2 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 1.132e-51
Identity = 99/193 (51.30%), Postives = 128/193 (66.32%), Query Frame = 2 
Query:   80 RKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            RKFFVGGNWK NG  + + ++++TLN  +                     K  L +   ++AQNC+    GAFTGE+S  M+ +    WVILGHSERR +  E ++ +G KVA+ALS G+ VIAC+GE L+QRE+G T  VV EQTKAIA+ + +W  VVLAYEPVWAIGTGK ATP QAQEVH +LR+W+KT
Sbjct:    4 RKFFVGGNWKMNGDKKSLGELINTLNSGKMNADTEVVCGAPAIYLDFARQK--LDAKIALSAQNCYKVAKGAFTGEISPAMIKDCGATWVILGHSERRHVFGECDELIGQKVAHALSEGIGVIACIGEKLDQREAGITEKVVFEQTKAIADNVKDWSKVVLAYEPVWAIGTGKTATPEQAQEVHKKLREWVKT 194

HSP 2 Score: 26.5646 bits (57), Expect = 1.132e-51
Identity = 13/20 (65.00%), Postives = 13/20 (65.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            NVS  VA S RIIYGG   G
Sbjct:  195 NVSEGVAQSVRIIYGGSVTG 214
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_RAT (Triosephosphate isomerase OS=Rattus norvegicus GN=Tpi1 PE=1 SV=2)

HSP 1 Score: 197.593 bits (501), Expect = 1.923e-51
Identity = 102/193 (52.85%), Postives = 127/193 (65.80%), Query Frame = 2 
Query:   77 ARKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLK 655
            +RKFFVGGNWK NG  + + +++ TLN A+                     K  L     VAAQNC+    GAFTGE+S  M+ +L   WV+LGHSERR +  ES++ +G KV +ALS GL VIAC+GE L++RE+G T  VV EQTKAIA+ + +W  VVLAYEPVWAIGTGK ATP QAQEVH +LR WLK
Sbjct:    4 SRKFFVGGNWKMNGRKKCLGELICTLNAAKLPADTEVVCAPPTAYIDFARQK--LDPKIAVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVLGHSERRHIFGESDELIGQKVNHALSEGLGVIACIGEKLDEREAGITEKVVFEQTKAIADNVKDWCKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLK 194

HSP 2 Score: 26.5646 bits (57), Expect = 1.923e-51
Identity = 13/20 (65.00%), Postives = 13/20 (65.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            NVS  VA  TRIIYGG   G
Sbjct:  196 NVSEGVAQCTRIIYGGSVTG 215
[Close]
BLAST of FC930632 vs. ExPASy Swiss-Prot
Match: TPIS_MOUSE (Triosephosphate isomerase OS=Mus musculus GN=Tpi1 PE=1 SV=3)

HSP 1 Score: 196.438 bits (498), Expect = 3.268e-51
Identity = 100/193 (51.81%), Postives = 126/193 (65.28%), Query Frame = 2 
Query:   80 RKFFVGGNWKCNGTSEEVKKIVSTLNEAEXXXXXXXXXXXXXXXXXXXXXKSLLRSDFHVAAQNCWVRKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLNESNDFVGDKVAYALSRGLKVIACVGETLEQRESGSTVAVVAEQTKAIAEKISNWDNVVLAYEPVWAIGTGKVATPAQAQEVHAELRKWLKT 658
            RKFFVGGNWK NG  + + +++ TLN A                      K  L     VAAQNC+    GAFTGE+S  M+ +L   WV+LGHSERR +  ES++ +G KV++AL+ GL VIAC+GE L++RE+G T  VV EQTK IA+ + +W  VVLAYEPVWAIGTGK ATP QAQEVH +LR WLK+
Sbjct:    5 RKFFVGGNWKMNGRKKCLGELICTLNAANVPAGTEVVCAPPTAYIDFARQK--LDPKIAVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVLGHSERRHVFGESDELIGQKVSHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKS 195

HSP 2 Score: 26.9498 bits (58), Expect = 3.268e-51
Identity = 13/20 (65.00%), Postives = 14/20 (70.00%), Query Frame = 1 
Query:  658 NVSAEVAASTRIIYGGFCNG 717
            NV+  VA STRIIYGG   G
Sbjct:  196 NVNDGVAQSTRIIYGGSVTG 215
The following BLAST results are available for this feature:
BLAST of FC930632 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIS_PANTR2.307e-5251.55Triosephosphate isomerase OS=Pan troglodytes GN=TP... [more]
TPIS_HUMAN2.307e-5251.55Triosephosphate isomerase OS=Homo sapiens GN=TPI1 ... [more]
TPIS_CHICK2.307e-5252.85Triosephosphate isomerase OS=Gallus gallus GN=TPI1... [more]
TPIS_BOVIN3.007e-5252.06Triosephosphate isomerase OS=Bos taurus GN=TPI1 PE... [more]
TPIS_PIG3.921e-5252.58Triosephosphate isomerase OS=Sus scrofa GN=TPI1 PE... [more]
TPISB_DANRE3.921e-5251.81Triosephosphate isomerase B OS=Danio rerio GN=tpi1... [more]
TPIS_GORGO6.662e-5251.03Triosephosphate isomerase OS=Gorilla gorilla goril... [more]
TPIS_XENLA1.132e-5151.30Triosephosphate isomerase OS=Xenopus laevis GN=tpi... [more]
TPIS_RAT1.923e-5152.85Triosephosphate isomerase OS=Rattus norvegicus GN=... [more]
TPIS_MOUSE3.268e-5151.81Triosephosphate isomerase OS=Mus musculus GN=Tpi1 ... [more]

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Properties
Property NameValue
Genbank descriptionC34009F04EF PostHarvC1 Citrus clementina cDNA clone C34009F04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930632 ID=FC930632; Name=FC930632; organism=Citrus clementina; type=EST; length=718bp
GACAACAGGGTGTTGATTTCTCGTATTTTCGTCTCGCTTTCATCGCCAAA
CCAAACAAAATTCAATCGTCGTCATGGCCCGCAAATTCTTCGTCGGCGGC
AACTGGAAATGCAATGGGACATCTGAGGAAGTGAAGAAGATTGTTTCTAC
ATTGAATGAAGCTGAAGTTCCTTCTGAGGATGTTGTGGAGGTTGTTGTAA
GCCCTCCGTTTGTGTTTCTTCCTTTGGTCAAGAGTCTGTTGCGGTCTGAT
TTCCATGTTGCCGCTCAAAATTGCTGGGTTCGCAAAGGTGGTGCTTTTAC
CGGGGAAGTTAGTGCTGAGATGCTTGTCAACTTGAGCATTCCTTGGGTCA
TTCTTGGTCATTCTGAAAGAAGAGCTCTTTTGAATGAGTCAAATGATTTT
GTTGGAGATAAAGTTGCTTATGCACTTTCTCGAGGCTTGAAAGTCATTGC
TTGTGTTGGAGAGACTCTGGAACAGCGAGAATCAGGATCTACAGTGGCTG
TTGTGGCTGAACAAACAAAAGCAATCGCAGAAAAAATATCCAACTGGGAC
AATGTTGTGTTGGCTTATGAGCCAGTTTGGGCCATTGGTACCGGGAAGGT
TGCAACCCCTGCTCAGGCTCAGGAAGTCCATGCTGAACTGAGGAAATGGC
TTAAGACAATGTCAGTGCTGAAGTTGCAGCATCAACTAGAATCATCTACG
GAGGTTTCTGTAATGGAG
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