FC930887

Overview
NameFC930887
Unique NameFC930887
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length683
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBE2C_SPISO (Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima GN=UBE2C PE=1 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 3.356e-25
Identity = 56/137 (40.88%), Postives = 86/137 (62.77%), Query Frame = 2
Query:  197 RVMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 607
            R   SKR+ +EL+ L        +A P  +++F W AT+ GP D+ Y    + +T+ FP DYP+KPP V F T  +HPN++ +G+ICLDILKE W+ +  +  +LLS+ SLL +PN   PL  + A M+ S++ +Y+
Sbjct:   28 RHSVSKRLQQELRTLLMSGDPGITAFPDGDNLFKWVATLDGPKDTVYESLKYKLTLEFPSDYPYKPPVVKFTTPCWHPNVDQSGNICLDILKENWTASYDVRTILLSLQSLLGEPNNASPLNAQAADMW-SNQTEYK 163          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBE2S_HUMAN (Ubiquitin-conjugating enzyme E2 S OS=Homo sapiens GN=UBE2S PE=1 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 7.476e-25
Identity = 56/138 (40.58%), Postives = 78/138 (56.52%), Query Frame = 2
Query:  218 ILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 631
            + KE+  L  DPP      P  ED+   Q TI GP  +PYAGG+F + +    D+P  PPK  F TK+FHPN+ +NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   16 VYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGANGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBE2S_BOVIN (Ubiquitin-conjugating enzyme E2 S OS=Bos taurus GN=UBE2S PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 7.476e-25
Identity = 56/138 (40.58%), Postives = 78/138 (56.52%), Query Frame = 2
Query:  218 ILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 631
            + KE+  L  DPP      P  ED+   Q TI GP  +PYAGG+F + +    D+P  PPK  F TK+FHPN+ +NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   16 VYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGANGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBE2C_DROME (Ubiquitin-conjugating enzyme E2 C OS=Drosophila melanogaster GN=vih PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 7.476e-25
Identity = 57/143 (39.86%), Postives = 88/143 (61.54%), Query Frame = 2
Query:  209 SKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKY 637
            SKR+ KEL +L        SA P  E++F W  TI GP ++ Y+G  + +++ FP  YP+  P V F T  FHPN++  G+ICLDILK++WS    +  +LLSI SLL +PN + PL  + A M+ +D+ +Y+    ++ +K+
Sbjct:   34 SKRLHKELMNLMMANERGISAFPDGENIFKWVGTIAGPRNTVYSGQTYRLSLDFPNSYPYAAPVVKFLTSCFHPNVDLQGAICLDILKDKWSALYDVRTILLSIQSLLGEPNNESPLNAQAAMMW-NDQKEYKKYLDAFYEKH 175          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBC11_SCHPO (Ubiquitin-conjugating enzyme E2-20 kDa OS=Schizosaccharomyces pombe GN=ubc11 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 9.763e-25
Identity = 52/134 (38.81%), Postives = 83/134 (61.94%), Query Frame = 2
Query:  209 SKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 607
            +KR+  EL  L        SA P ++ ++ HW  TI GPSD+ Y G  F +++ FP +YP+ PP + F + ++HPN++ +G+ICLDILK++WS    +  +LLS+ SLL +PN   PL  + A ++  D  +Y+
Sbjct:   30 TKRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDILKDKWSAVYNVQTILLSLQSLLGEPNNASPLNAQAAELWSKDPIEYK 163          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBE2S_RAT (Ubiquitin-conjugating enzyme E2 S OS=Rattus norvegicus GN=Ube2s PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.275e-24
Identity = 56/138 (40.58%), Postives = 77/138 (55.80%), Query Frame = 2
Query:  218 ILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 631
            + KE+  L  DPP      P  ED+   Q TI GP  +PYAGG+F + +    D+P  PPK  F TK+FHPN+  NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   16 VYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGPNGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBE2S_MOUSE (Ubiquitin-conjugating enzyme E2 S OS=Mus musculus GN=Ube2s PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.275e-24
Identity = 56/138 (40.58%), Postives = 77/138 (55.80%), Query Frame = 2
Query:  218 ILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 631
            + KE+  L  DPP      P  ED+   Q TI GP  +PYAGG+F + +    D+P  PPK  F TK+FHPN+  NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   16 VYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGPNGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UB2SA_XENLA (Ubiquitin-conjugating enzyme E2 S-A OS=Xenopus laevis GN=ube2s-A PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.275e-24
Identity = 56/138 (40.58%), Postives = 77/138 (55.80%), Query Frame = 2
Query:  218 ILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 631
            + KE+  L  DPP      P  ED+   Q TI GP  +PYAGG+F + +    D+P  PPK  F TK+FHPN+  NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   16 VYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGPNGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBC15_SCHPO (Ubiquitin-conjugating enzyme E2 15 OS=Schizosaccharomyces pombe GN=ubc15 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.665e-24
Identity = 54/153 (35.29%), Postives = 90/153 (58.82%), Query Frame = 2
Query:  206 ASKRIL-KELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILK-------------EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTAR 619
            AS+++L K+LK++QK+PP   S G V +  +F W+  I+GP D+ Y GG F  T+ FP DYP  PPK+ F T+++HPN++ NG +C+ IL              E+W P  +   +L+S+ S+L+ PN + P   + A  ++ +  +++   R
Sbjct:    5 ASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPETILISVISMLSSPNDESPANIDAAKEFRENPQEFKKRVR 157          
BLAST of FC930887 vs. ExPASy Swiss-Prot
Match: UBE2S_DROGR (Ubiquitin-conjugating enzyme E2 S OS=Drosophila grimshawi GN=GH12079 PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.841e-24
Identity = 57/143 (39.86%), Postives = 80/143 (55.94%), Query Frame = 2
Query:  212 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 640
            ++++KEL+D++  PP          D+   QA I GP+ +PYA G+F V +    D+P  PPK  F TK+FHPN+ SNG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   17 RQVMKELQDMESTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHPNVASNGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159          
The following BLAST results are available for this feature:
BLAST of FC930887 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 267
Match NameE-valueIdentityDescription
UBE2C_SPISO3.356e-2540.88Ubiquitin-conjugating enzyme E2 C OS=Spisula solid... [more]
UBE2S_HUMAN7.476e-2540.58Ubiquitin-conjugating enzyme E2 S OS=Homo sapiens ... [more]
UBE2S_BOVIN7.476e-2540.58Ubiquitin-conjugating enzyme E2 S OS=Bos taurus GN... [more]
UBE2C_DROME7.476e-2539.86Ubiquitin-conjugating enzyme E2 C OS=Drosophila me... [more]
UBC11_SCHPO9.763e-2538.81Ubiquitin-conjugating enzyme E2-20 kDa OS=Schizosa... [more]
UBE2S_RAT1.275e-2440.58Ubiquitin-conjugating enzyme E2 S OS=Rattus norveg... [more]
UBE2S_MOUSE1.275e-2440.58Ubiquitin-conjugating enzyme E2 S OS=Mus musculus ... [more]
UB2SA_XENLA1.275e-2440.58Ubiquitin-conjugating enzyme E2 S-A OS=Xenopus lae... [more]
UBC15_SCHPO1.665e-2435.29Ubiquitin-conjugating enzyme E2 15 OS=Schizosaccha... [more]
UBE2S_DROGR2.841e-2439.86Ubiquitin-conjugating enzyme E2 S OS=Drosophila gr... [more]

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Properties
Property NameValue
Genbank descriptionC34110B03EF PostHarvP1 Citrus clementina cDNA clone C34110B03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930887 ID=FC930887; Name=FC930887; organism=Citrus clementina; type=EST; length=683bp
GTTTCTCGACGATATTTGTGTTGAAGGACAACAAAGCAAGGGGTTGATAG
AGGAGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCG
CTTTCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGTTTC
GCAGGTCTTAAGGTTCGTTGTAGTTTCAATTTTAATAATCCCTTGAAGAG
TCATGGCTTCAAAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGAT
CCTCCCACGTCTTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTG
GCAAGCAACAATTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTAT
TTTTAGTTACCATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAG
GTTGCTTTTAGGACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAG
CATCTGTCTTGATATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTT
CAAAGGTCCTGCTCTCTATCTGCTCCTTGCTTACTGATCCAAATCCCGAC
GACCCTCTTGTACCGGAGATTGCCCACATGTACAAGTCCGACAAGGCCAA
ATATGAATCCACGGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAA
GGTTTGTAGGATATTGCAGAAAAGGAGCATTTA
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