FC930944 | Citrus Genome Database

Overview


Name	FC930944
Unique Name	FC930944
Type	EST
Organism	Citrus clementina (Clementine)
Sequence length	682

Libraries


Library Name	Type
PostHarvP1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_METTH (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Methanobacterium thermoautotrophicum GN=sucD PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.495e-23
Identity = 26/43 (60.47%), Postives = 35/43 (81.40%), Query Frame = 3

Query:  255 VFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            + +D++TR + QGITGK G+FHT Q +EYGT++V GVTP KGG
Sbjct:    7 ILLDEDTRCLVQGITGKQGSFHTRQMLEYGTRIVAGVTPGKGG 49

HSP 2 Score: 60.4622 bits (145), Expect = 4.495e-23
Identity = 36/81 (44.44%), Postives = 46/81 (56.79%), Query Frame = 2

Query:  374 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGP 616
            +G  E LG+ V+NSV E   E   NAS+I+VP PF            LDLVV ITE IP HD +++    +R  KT +IGP
Sbjct:   47 KGGQEFLGVDVYNSVEEVTEEMDVNASIIFVPAPF-AKDAAFESIKHLDLVVIITEHIPVHDSMQIMEYASRMGKT-VIGP 125

HSP 3 Score: 24.2534 bits (51), Expect = 4.495e-23
Identity = 11/18 (61.11%), Postives = 13/18 (72.22%), Query Frame = 1

Query:  622 PGIIKPGEM*IGIMPGYI 675
            PGII PG   +GIMP +I
Sbjct:  128 PGIITPGIGKLGIMPTHI 145

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCA2_TRIVA (Succinyl-CoA ligase [GDP-forming] subunit alpha-2, mitochondrial OS=Trichomonas vaginalis GN=ALPHA-SCS2 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 7.822e-22
Identity = 33/74 (44.59%), Postives = 46/74 (62.16%), Query Frame = 2

Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGP 616
            GLP+F ++ E    T ANAS+I+VP P             + LVVCITE IPQHDM++VK  + +++  +LIGP
Sbjct:   61 GLPIFKNMKEVVKRTDANASLIFVPAPGAAAACIEAAEAGMGLVVCITEHIPQHDMIKVKKVM-KETGCQLIGP 133

HSP 2 Score: 55.8398 bits (133), Expect = 7.822e-22
Identity = 26/46 (56.52%), Postives = 33/46 (71.74%), Query Frame = 3

Query:  246 QPAVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            QP +F+DK+TRV+ QGI G  G +H+    EYGTK+VG V PKK G
Sbjct:   12 QPLLFIDKDTRVVIQGI-GNQGQYHSRLMREYGTKVVGAVHPKKAG 56

HSP 3 Score: 22.7126 bits (47), Expect = 7.822e-22
Identity = 10/23 (43.48%), Postives = 16/23 (69.57%), Query Frame = 1

Query:  616 HCPGIIKPG-EM*IGIMPGYIHK 681
            +CPG+I+PG    +GI+P  I +
Sbjct:  134 NCPGLIQPGTHTKLGIIPTNIFR 156

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCA_HUMAN (Succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial OS=Homo sapiens GN=SUCLG1 PE=1 SV=4)

HSP 1 Score: 103.605 bits (257), Expect = 1.010e-21
Identity = 58/106 (54.72%), Postives = 66/106 (62.26%), Query Frame = 2

Query:  353 GGWRDAEEGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            GG    + G T HLGLPVFN+V EAK +T A ASVIYVPPPF            + LVVCITEGIPQ DMVRVK  L RQ KTRLIGP      N  +C++ +  G
Sbjct:   84 GGTTPGKGGQT-HLGLPVFNTVKEAKEQTGATASVIYVPPPFAAAAINEAIEAEIPLVVCITEGIPQQDMVRVKHKLLRQEKTRLIGPNCPGVINPGECKIGIMPG 188

HSP 2 Score: 79.7221 bits (195), Expect = 1.549e-14
Identity = 34/51 (66.67%), Postives = 43/51 (84.31%), Query Frame = 3

Query:  231 SAAASQPAVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            S  AS+  ++VDKNT++ICQG TGK GTFH++QA+EYGTK+VGG TP KGG
Sbjct:   42 SYTASRQHLYVDKNTKIICQGFTGKQGTFHSQQALEYGTKLVGGTTPGKGG 92

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCA3_TRIVA (Succinyl-CoA ligase [GDP-forming] subunit alpha-3, mitochondrial OS=Trichomonas vaginalis GN=ALPHA-SCS3 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.210e-21
Identity = 33/74 (44.59%), Postives = 46/74 (62.16%), Query Frame = 2

Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGP 616
            GLP+F ++ E    T ANAS+I+VP P             + LVVCITE IPQHDM++VK  + +++  +LIGP
Sbjct:   61 GLPIFKNMKEVVKRTDANASLIFVPAPGAAAACIEAAQAGMGLVVCITEHIPQHDMIKVKKVM-KETGCQLIGP 133

HSP 2 Score: 54.6842 bits (130), Expect = 2.210e-21
Identity = 25/46 (54.35%), Postives = 33/46 (71.74%), Query Frame = 3

Query:  246 QPAVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            QP +F+DK+T+V+ QGI G  G FH+    +YGTK+VG V PKK G
Sbjct:   12 QPLLFIDKDTKVVIQGI-GNQGQFHSRLMRQYGTKVVGAVHPKKAG 56

HSP 3 Score: 22.3274 bits (46), Expect = 2.210e-21
Identity = 9/18 (50.00%), Postives = 14/18 (77.78%), Query Frame = 1

Query:  616 HCPGIIKPG-EM*IGIMP 666
            +CPG+I+PG    +GI+P
Sbjct:  134 NCPGLIQPGTHTKLGIIP 151

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCA1_TRIVA (Succinyl-CoA ligase [GDP-forming] subunit alpha-1, mitochondrial OS=Trichomonas vaginalis GN=ALPHA-SCS1 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 6.244e-21
Identity = 33/74 (44.59%), Postives = 46/74 (62.16%), Query Frame = 2

Query:  395 GLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGP 616
            GLP+F ++ E    T ANAS+I+VP P             + LVVCITE IPQHDM++VK  + +++  +LIGP
Sbjct:   61 GLPIFKNMKEVVKRTDANASLIFVPAPGAAAACIEAAEAGMGLVVCITEHIPQHDMIKVKKVM-KETGCQLIGP 133

HSP 2 Score: 53.1434 bits (126), Expect = 6.244e-21
Identity = 24/46 (52.17%), Postives = 33/46 (71.74%), Query Frame = 3

Query:  246 QPAVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            +P +F+DK+T+V+ QGI G  G FH+    +YGTK+VG V PKK G
Sbjct:   12 KPLLFIDKDTKVVIQGI-GNQGQFHSRLMRQYGTKVVGAVHPKKAG 56

HSP 3 Score: 22.3274 bits (46), Expect = 6.244e-21
Identity = 9/18 (50.00%), Postives = 14/18 (77.78%), Query Frame = 1

Query:  616 HCPGIIKPG-EM*IGIMP 666
            +CPG+I+PG    +GI+P
Sbjct:  134 NCPGLIQPGTHTKLGIIP 151

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_THETH (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Thermus thermophilus GN=sucD PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.165e-20
Identity = 26/43 (60.47%), Postives = 35/43 (81.40%), Query Frame = 3

Query:  255 VFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            + V++ TRV+ QGITG+ G FHT+Q ++YGTK+V GVTP KGG
Sbjct:    2 ILVNRETRVLVQGITGREGQFHTKQMLDYGTKIVAGVTPGKGG 44

HSP 2 Score: 56.9954 bits (136), Expect = 1.165e-20
Identity = 35/99 (35.35%), Postives = 54/99 (54.55%), Query Frame = 2

Query:  374 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            +G TE LG+PV+++V EA A  + +AS+I+VP P             + L+V ITEGIP  DMVR    + +   +RLIG       + E+ ++ +  G
Sbjct:   42 KGGTEVLGVPVYDTVKEAVAHHEVDASIIFVPAPAAADAALEAAHAGIPLIVLITEGIPTLDMVRAVEEI-KALGSRLIGGNCPGIISAEETKIGIMPG 139

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_RICBR (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Rickettsia bellii (strain RML369-C) GN=sucD PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.973e-18
Identity = 50/99 (50.51%), Postives = 64/99 (64.65%), Query Frame = 2

Query:  374 EGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            +G   HL LP++N+V EAKA+T ANASVIYVPPPF            +++VVCITEGIP  DMVRVK AL   S+TRLIGP        ++C++ +  G
Sbjct:   43 KGGKTHLDLPIYNTVHEAKAKTGANASVIYVPPPFAADSILEAIDAGIEIVVCITEGIPVLDMVRVKRAL-VGSRTRLIGPNCPGIITPDECKIGIMPG 140

HSP 2 Score: 75.8702 bits (185), Expect = 2.236e-13
Identity = 36/55 (65.45%), Postives = 43/55 (78.18%), Query Frame = 3

Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGLSTWDF-LYST 413
            A+ V+K T+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGG +  D  +Y+T
Sbjct:    2 AILVNKKTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGGKTHLDLPIYNT 56

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_MYCTU (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Mycobacterium tuberculosis GN=sucD PE=3 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.073e-18
Identity = 34/74 (45.95%), Postives = 41/74 (55.41%), Query Frame = 2

Query:  398 LPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKA-ALNRQSKTRLIGP 616
            LPVF SVAEA  +T A+ S+I+VPP F            + L+V ITEGIP  D     A  L    KTR+IGP
Sbjct:   63 LPVFGSVAEAMEKTGADVSIIFVPPTFAKDAIIEAIDAEIPLLVVITEGIPVQDTAYAWAYNLEAGHKTRIIGP 136

HSP 2 Score: 46.9802 bits (110), Expect = 4.073e-18
Identity = 19/44 (43.18%), Postives = 30/44 (68.18%), Query Frame = 3

Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            ++F+ ++ +VI QGITG   T HT + +  GT++VGGV  +K G
Sbjct:    5 SIFLSRDNKVIVQGITGSEATVHTARMLRAGTQIVGGVNARKAG 48

HSP 3 Score: 26.9498 bits (58), Expect = 4.073e-18
Identity = 11/20 (55.00%), Postives = 13/20 (65.00%), Query Frame = 1

Query:  616 HCPGIIKPGEM*IGIMPGYI 675
            +CPGII PG+   GI P  I
Sbjct:  137 NCPGIISPGQSLAGITPANI 156

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_MYCBO (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Mycobacterium bovis GN=sucD PE=3 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.073e-18
Identity = 34/74 (45.95%), Postives = 41/74 (55.41%), Query Frame = 2

Query:  398 LPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKA-ALNRQSKTRLIGP 616
            LPVF SVAEA  +T A+ S+I+VPP F            + L+V ITEGIP  D     A  L    KTR+IGP
Sbjct:   63 LPVFGSVAEAMEKTGADVSIIFVPPTFAKDAIIEAIDAEIPLLVVITEGIPVQDTAYAWAYNLEAGHKTRIIGP 136

HSP 2 Score: 46.9802 bits (110), Expect = 4.073e-18
Identity = 19/44 (43.18%), Postives = 30/44 (68.18%), Query Frame = 3

Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            ++F+ ++ +VI QGITG   T HT + +  GT++VGGV  +K G
Sbjct:    5 SIFLSRDNKVIVQGITGSEATVHTARMLRAGTQIVGGVNARKAG 48

HSP 3 Score: 26.9498 bits (58), Expect = 4.073e-18
Identity = 11/20 (55.00%), Postives = 13/20 (65.00%), Query Frame = 1

Query:  616 HCPGIIKPGEM*IGIMPGYI 675
            +CPGII PG+   GI P  I
Sbjct:  137 NCPGIISPGQSLAGITPANI 156

[Close]

BLAST of FC930944 vs. ExPASy Swiss-Prot
Match: SUCD_RICTY (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Rickettsia typhi GN=sucD PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 7.493e-17
Identity = 53/106 (50.00%), Postives = 65/106 (61.32%), Query Frame = 2

Query:  353 GGWRDAEEGWTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPTALVSSNLEKCRLALCLG 670
            GG    + G T HL LPV+N+V EAKA+T ANASVIYVPP F            +++VVCITEGIP  DMV+VK AL   SKTRLIGP         +C++ +  G
Sbjct:   37 GGVTPGKGGHT-HLNLPVYNTVHEAKAKTDANASVIYVPPGFAADSILEAIDAKIEVVVCITEGIPVLDMVKVKRAL-IGSKTRLIGPNCPGVITPGECKIGIMPG 140

HSP 2 Score: 72.0182 bits (175), Expect = 3.229e-12
Identity = 31/44 (70.45%), Postives = 36/44 (81.82%), Query Frame = 3

Query:  252 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGG 383
            A+ ++K T+VICQG TG  GTFH+EQAI YGT MVGGVTP KGG
Sbjct:    2 AILINKKTKVICQGFTGSQGTFHSEQAIAYGTNMVGGVTPGKGG 45

The following BLAST results are available for this feature:

BLAST of FC930944 vs. ExPASy Swiss-Prot

BLAST of FC930944 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45


Match Name	E-value	Identity	Description
SUCD_METTH	4.495e-23	60.47	Succinyl-CoA ligase [ADP-forming] subunit alpha OS...	[more]
SUCA2_TRIVA	7.822e-22	44.59	Succinyl-CoA ligase [GDP-forming] subunit alpha-2,...	[more]
SUCA_HUMAN	1.010e-21	54.72	Succinyl-CoA ligase [GDP-forming] subunit alpha, m...	[more]
SUCA3_TRIVA	2.210e-21	44.59	Succinyl-CoA ligase [GDP-forming] subunit alpha-3,...	[more]
SUCA1_TRIVA	6.244e-21	44.59	Succinyl-CoA ligase [GDP-forming] subunit alpha-1,...	[more]
SUCD_THETH	1.165e-20	60.47	Succinyl-CoA ligase [ADP-forming] subunit alpha OS...	[more]
SUCD_RICBR	3.973e-18	50.51	Succinyl-CoA ligase [ADP-forming] subunit alpha OS...	[more]
SUCD_MYCTU	4.073e-18	45.95	Succinyl-CoA ligase [ADP-forming] subunit alpha OS...	[more]
SUCD_MYCBO	4.073e-18	45.95	Succinyl-CoA ligase [ADP-forming] subunit alpha OS...	[more]
SUCD_RICTY	7.493e-17	50.00	Succinyl-CoA ligase [ADP-forming] subunit alpha OS...	[more]

Pages

Properties


Property Name	Value
Genbank description	C34110G01EF PostHarvP1 Citrus clementina cDNA clone C34110G01, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FC930944 ID=FC930944; Name=FC930944; organism=Citrus clementina; type=EST; length=682bp
CGGGGGAGAATCTTTTTCTTCAATATATTTTGGGAAAAAAGAGAGAGACG
AACTCGAGGGAGAAAATCAGCGAATCAGATTAAAGTGAAGTGAATCAAAT
GGCTCGCCAAGCCTTGGCCAAGCTGATCGGCTCGATCGGATGTCGCAGAC
CCTCTACTGCTTCTTCCGTTTCACATTACAGATTATCAACGTCGGCGGCG
CCGGCGGCCCACTCTCGATATTATGGGTCGTCGGCGGCGGCGTCTCAGCC
GGCAGTTTTCGTCGACAAAAACACTCGAGTTATATGCCAAGGCATCACCG
GAAAAAACGGCACGTTCCATACCGAACAAGCCATCGAATACGGCACCAAA
ATGGTGGGTGGCGTGACGCCGAAGAAGGGTGGACTGAGCACTTGGGACTT
CCTGTATTCAACTCGGTTGCTGAAGCTAAGGCTGAAACGAAGGCTAATGC
TTCTGTCATTTATGTTCCTCCGCCATTTGCTGCTGCTGCTATCATGGAGG
CTATGGAGGCTGAGTTGGATCTTGTCGTCTGTATCACCGAAGGAATCCCT
CAACACGATATGGTGCGAGTGAAAGCTGCTCTAAACAGGCAATCTAAAAC
TCGGCTGATTGGTCCCACTGCCCTGGTATCATCAAACCTGGAGAAATGTA
GATTGGCATTATGCCTGGGTATATTCACAAAC