FC931255

Overview
NameFC931255
Unique NameFC931255
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length698
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: CFTR_RAT (Cystic fibrosis transmembrane conductance regulator OS=Rattus norvegicus GN=Cftr PE=1 SV=3)

HSP 1 Score: 140.584 bits (353), Expect = 7.785e-33
Identity = 74/225 (32.89%), Postives = 125/225 (55.56%), Query Frame = 1
Query:   22 SWPSHGKIDLRDLQVRYAPHMPFVLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHGLIEEYDS 696
            +WPS G++ ++DL V+Y      +L  IS +    ++ G++GRTGSGKSTL+    R++                      R    +I Q   +F GT R N+DP  ++ DE+IW+  D+  L   + +  G+L+  + + G   S G +QL+CL R +L + KI++LDE +A++D  T  +I++ LRQ F+ CTV+   HRI +++D    L++  G + +Y+S
Sbjct: 1199 TWPSGGEMVVKDLTVKYVDDGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLN-IKGEIQIDGVSWNSMTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWRDEEIWKVADQVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSANLDPITYQVIRRVLRQAFAGCTVVLCEHRIEAMLDCQRFLVIEQGNVWQYES 1422          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: CFTR_SQUAC (Cystic fibrosis transmembrane conductance regulator OS=Squalus acanthias GN=CFTR PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.265e-32
Identity = 71/224 (31.70%), Postives = 126/224 (56.25%), Query Frame = 1
Query:   25 WPSHGKIDLRDLQVRYAPHMPFVLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHGLIEEYDS 696
            WPS G++ + +L  +Y      VL+ +S +    ++ G++GRTG+GKSTL+  L R++                      R    +IPQ   +F GT R N+DP E+++DE+IW+  ++  L   + +   KL+  + + G   S G +QL+CL R +L + KIL+LDE TA +D  T  +I+++L+  FS+CTVI   HR+ ++++    L++    ++++D+
Sbjct: 1214 WPSGGQMMVNNLTAKYTSDGRAVLQDLSFSVNAGQRVGLLGRTGAGKSTLLSALLRLLSTEGEIQIDGISWNSVSLQKW-RKAFGVIPQKVFVFSGTFRKNLDPYEQWSDEEIWKVTEEVGLKSMIEQFPDKLNFVLVDGGYILSNGHKQLMCLARSILSKAKILLLDEPTAHLDPVTFQIIRKTLKHTFSNCTVILSEHRVEALLECQQFLVIEGCSVKQFDA 1436          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: CFTR_MOUSE (Cystic fibrosis transmembrane conductance regulator OS=Mus musculus GN=Cftr PE=1 SV=2)

HSP 1 Score: 137.887 bits (346), Expect = 5.046e-32
Identity = 75/224 (33.48%), Postives = 122/224 (54.46%), Query Frame = 1
Query:   25 WPSHGKIDLRDLQVRYAPHMPFVLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHGLIEEYDS 696
            WPS G++ ++DL V+Y      VL  IS +    ++ G++GRTGSGKSTL+    R++                      R    +I Q   +F GT R N+DP  ++ DE+IW+  D+  L   + +  G+L+  + + G   S G +QL+CL R +L + KI++LDE +A +D  T  +I++ L+Q F+ CTVI   HRI +++D    L++    + +YDS
Sbjct: 1200 WPSGGEMVVKDLTVKYMDDGNAVLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLN-IKGDIEIDGVSWNSVTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAMLDCQRFLVIEESNVWQYDS 1422          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: CFTR_XENLA (Cystic fibrosis transmembrane conductance regulator OS=Xenopus laevis GN=cftr PE=2 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.243e-30
Identity = 71/224 (31.70%), Postives = 123/224 (54.91%), Query Frame = 1
Query:   25 WPSHGKIDLRDLQVRYAPHMPFVLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHGLIEEYDS 696
            WPS G++ +++L   Y      VL  IS +    ++ G++GRTGSGKSTL+    R++                      R    +IPQ   +F G++R N+DP  +++DE++ +  ++  L   + +  G+LD  + + G   S G +QLVCL R +L + KIL+LDE +A +D  T  +I+++L+  F+DCTVI   HR+ ++++    L++    + +YDS
Sbjct: 1207 WPSGGQMTVKNLSANYIDGGNTVLENISFSLSPGQRVGLLGRTGSGKSTLLSAFLRLLSTQGDIQIDGVSWQTIPLQKW-RKAFGVIPQKVFIFSGSIRKNLDPYGKWSDEELLKVTEEVGLKLIIDQFPGQLDFVLLDGGCVLSHGHKQLVCLARSVLSKAKILLLDEPSAHLDPITFQIIRKTLKHAFADCTVILSEHRLEAMLECQRFLVIEDNTVRQYDS 1429          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: MDLB_BUCAP (Multidrug resistance-like ATP-binding protein mdlB OS=Buchnera aphidicola subsp. Schizaphis graminum GN=mdlB PE=3 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 2.344e-29
Identity = 71/217 (32.72%), Postives = 118/217 (54.38%), Query Frame = 1
Query:   37 GKIDLRDLQVRYAPHMPFVLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHGLIEE 687
            GKI++++L  +Y      +L  I+   P +     VG+TGSGKSTL   L                          R  + ++ QDP++   T  SN+   ++ ++E+IW  L    L   V+     + S + E G N S+GQ+QL+ + R+L++  KIL+LDEATA++D+ T+ LIQ +L    +  T++ IAHR+++VI++DM+++L  G I E
Sbjct:  339 GKINIKNLSFKYKESGENILNNINIYIPSKSFVAFVGQTGSGKSTLANLLMGYYPIKHGKIYLDDKSINCISHDVLRKNILMVQQDPIVLADTFSSNITLGKKISEEKIWNVLKTVHLSSLVQSMPKGIYSILGEEGNNLSLGQKQLLAIARILVRNPKILILDEATANIDSGTEKLIQTTLSSIRAKTTLVVIAHRLSTVIEADMIVVLKKGKIVE 555          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: AB9B_ARATH (ABC transporter B family member 9 OS=Arabidopsis thaliana GN=ABCB9 PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 9.848e-28
Identity = 70/220 (31.82%), Postives = 122/220 (55.45%), Query Frame = 1
Query:   37 GKIDLRDLQVRYAPHMPFV--LRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVD-PLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHGLIEE 687
            G I+L+D+  RY P  P V    G S   P  K   +VG++GSGKST+I  + R  +P +                  R+++ ++ Q+PV+F  T++ N+    E+ TD++I  A++       + K    LD+ V E+G   S GQ+Q + + R +LK  KIL+LDEAT+++D  ++ ++Q +L    S+ T + +AHR+T++  +D++ ++  G I E
Sbjct:  353 GDIELKDVYFRY-PARPDVQIFAGFSLFVPNGKTVALVGQSGSGKSTVISLIERFYDPESGQVLIDNIDLKKLQLKWIRSKIGLVSQEPVLFATTIKENIAYGKEDATDQEIRTAIELANAAKFIDKLPQGLDTMVGEHGTQMSGGQKQRLAIARAILKNPKILLLDEATSALDAESERIVQDALVNLMSNRTTVVVAHRLTTIRTADVIAVVHQGKIVE 571          

HSP 2 Score: 114.005 bits (284), Expect = 7.803e-25
Identity = 66/222 (29.73%), Postives = 117/222 (52.70%), Query Frame = 1
Query:   34 HGKIDLRDLQVRYAPHMPFV--LRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNV--DPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHGLIEE 687
            +G I+ R +  RY P  P V   R +  T P  K   +VG +GSGKST+I  + R   P +                  R ++ ++ Q+P++F  T+RSN+        T+E+I  A       + +       D+ V E G   S GQ+Q + + R +LK  KIL+LDEAT+++D  ++ ++Q +L +   + T + +AHR+T++ ++D++ ++ +G+I E
Sbjct: 1032 NGDIEFRHVSFRY-PMRPDVQIFRDLCLTIPSGKTVALVGESGSGKSTVISMIERFYNPDSGKILIDQVEIQTFKLSWLRQQMGLVSQEPILFNETIRSNIAYGKTGGATEEEIIAAAKAANAHNFISSLPQGYDTSVGERGVQLSGGQKQRIAIARAILKDPKILLLDEATSALDAESERVVQDALDRVMVNRTTVVVAHRLTTIKNADVIAVVKNGVIAE 1252          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: MDLB_ECOLI (Multidrug resistance-like ATP-binding protein mdlB OS=Escherichia coli (strain K12) GN=mdlB PE=1 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.286e-27
Identity = 66/195 (33.85%), Postives = 108/195 (55.38%), Query Frame = 1
Query:   91 VLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHG 675
            VL+ I+ + P      +VG TGSGKSTL   L                          R  ++++ QDPV+   T  +NV    + ++E++W+AL+  QL +  R     + + + E G N S+GQ+QL+ L RVL++  +IL+LDEATAS+D+ T+  IQ +L       T++ IAHR+++++D+D +L+L  G
Sbjct:  356 VLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYPLTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQALETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASIDSGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRG 550          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: MDLB_ECOL6 (Multidrug resistance-like ATP-binding protein mdlB OS=Escherichia coli O6 GN=mdlB PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.286e-27
Identity = 66/195 (33.85%), Postives = 108/195 (55.38%), Query Frame = 1
Query:   91 VLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHG 675
            VL+ I+ + P      +VG TGSGKSTL   L                          R  ++++ QDPV+   T  +NV    + ++E++W+AL+  QL +  R     + + + E G N S+GQ+QL+ L RVL++  +IL+LDEATAS+D+ T+  IQ +L       T++ IAHR+++++D+D +L+L  G
Sbjct:  356 VLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYPLTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQALETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASIDSGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRG 550          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: MDLB_ECO57 (Multidrug resistance-like ATP-binding protein mdlB OS=Escherichia coli O157:H7 GN=mdlB PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.286e-27
Identity = 66/195 (33.85%), Postives = 108/195 (55.38%), Query Frame = 1
Query:   91 VLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHG 675
            VL+ I+ + P      +VG TGSGKSTL   L                          R  ++++ QDPV+   T  +NV    + ++E++W+AL+  QL +  R     + + + E G N S+GQ+QL+ L RVL++  +IL+LDEATAS+D+ T+  IQ +L       T++ IAHR+++++D+D +L+L  G
Sbjct:  356 VLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYPLTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQALETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASIDSGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRG 550          
BLAST of FC931255 vs. ExPASy Swiss-Prot
Match: MDLB_BUCAI (Multidrug resistance-like ATP-binding protein mdlB OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=mdlB PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 4.888e-27
Identity = 67/218 (30.73%), Postives = 115/218 (52.75%), Query Frame = 1
Query:   37 GKIDLRDLQVRYAPHMPFVLRGISCTFPGEKKTGIVGRTGSGKSTLIQTLFRIVEPAAXXXXXXXXXXXXXXXXXXRTRLSIIPQDPVMFEGTVRSNVDPLEEYTDEQIWEALDKCQLGDEVRKKEGKLDSKVTENGENWSMGQRQLVCLGRVLLKRRKILMLDEATASVDTATDNLIQQSLRQHFSDCTVITIAHRITSVIDSDMVLLLSHGLIEEY 690
            GKI+++++   +      +L  I+     +     VG TGSGKSTL   +                          R  + ++ QDP++   T   N+    +  +E++W  LD   L D V+     + S + E G N S+GQ+QL+ + RVL+   K+L+LDEATA++D+ T+ LIQ++L     +CT+I IAHR++++I++D +++L  G I E+
Sbjct:  339 GKINIQNVSFYHKNCNKNILENINIKISSKSFVAFVGHTGSGKSTLANLIMGYYPLKNGKIYLDDKSIDSISHSVLRRNVLMVQQDPIVLSDTFFYNITLGRKIPEEKVWNILDTVHLSDLVKSMPKGIYSLLGEEGNNLSVGQKQLLAIARVLVAYPKVLILDEATANIDSGTEQLIQKTLLSIRKNCTLIIIAHRLSTIIEADSIIVLKKGKIVEF 556          
The following BLAST results are available for this feature:
BLAST of FC931255 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 455
Match NameE-valueIdentityDescription
CFTR_RAT7.785e-3332.89Cystic fibrosis transmembrane conductance regulato... [more]
CFTR_SQUAC2.265e-3231.70Cystic fibrosis transmembrane conductance regulato... [more]
CFTR_MOUSE5.046e-3233.48Cystic fibrosis transmembrane conductance regulato... [more]
CFTR_XENLA1.243e-3031.70Cystic fibrosis transmembrane conductance regulato... [more]
MDLB_BUCAP2.344e-2932.72Multidrug resistance-like ATP-binding protein mdlB... [more]
AB9B_ARATH9.848e-2831.82ABC transporter B family member 9 OS=Arabidopsis t... [more]
MDLB_ECOLI1.286e-2733.85Multidrug resistance-like ATP-binding protein mdlB... [more]
MDLB_ECOL61.286e-2733.85Multidrug resistance-like ATP-binding protein mdlB... [more]
MDLB_ECO571.286e-2733.85Multidrug resistance-like ATP-binding protein mdlB... [more]
MDLB_BUCAI4.888e-2730.73Multidrug resistance-like ATP-binding protein mdlB... [more]

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Properties
Property NameValue
Genbank descriptionC34103B10EF PostHarvP1 Citrus clementina cDNA clone C34103B10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931255 ID=FC931255; Name=FC931255; organism=Citrus clementina; type=EST; length=698bp
AGAAAAAGTCGGCCCAACCATTCTTGGCCATCACATGGGAAGATTGACCT
GCGTGATCTTCAGGTTCGCTATGCACCACACATGCCCTTTGTGTTGCGAG
GTATCTCGTGTACTTTCCCAGGGGAAAAGAAAACTGGTATTGTAGGGAGG
ACGGGCAGTGGTAAATCAACTCTCATTCAAACACTGTTCCGGATTGTTGA
ACCTGCTGCTGGTCAGATTCTTATAGATGGCATCGATATCTCATTGATTG
GACTACATGATTTGCGGACAAGACTAAGCATTATTCCCCAGGATCCTGTC
ATGTTTGAAGGGACTGTGCGAAGCAATGTAGACCCTCTTGAAGAATATAC
GGATGAGCAAATATGGGAGGCTCTGGATAAATGCCAACTTGGAGATGAAG
TTAGGAAGAAGGAAGGGAAACTAGATTCAAAAGTTACTGAGAATGGAGAG
AACTGGAGCATGGGCCAGAGACAGCTGGTATGTCTTGGCCGTGTGCTACT
GAAAAGAAGAAAAATCTTGATGCTTGATGAAGCTACGGCATCTGTTGACA
CTGCTACTGATAATCTGATCCAACAAAGTCTCCGGCAGCACTTCTCTGAC
TGCACAGTCATAACCATTGCTCATCGGATAACGTCTGTTATTGACAGTGA
TATGGTTCTGCTTCTCAGTCATGGACTCATCGAGGAATATGATTCCCC
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