FC931559

Overview
NameFC931559
Unique NameFC931559
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length683
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC931559_ssr95 microsatellite FC931559_ssr95:95..106. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig584 contig Ccv1_Contig584:11..694. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_STAAC (Ribose-phosphate pyrophosphokinase OS=Staphylococcus aureus (strain COL) GN=prs PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.628e-16
Identity = 34/66 (51.52%), Postives = 51/66 (77.27%), Query Frame = 2 
Query:  443 NRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N   N+ +K+FS   N AL+QE+A  +G+ELGK ++KRF+DGEI + ++ES+RGCDV+++QPT  P
Sbjct:    4 NEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSDGEIQINIEESIRGCDVFIIQPTSYP 69

HSP 2 Score: 26.1794 bits (56), Expect = 1.628e-16
Identity = 15/20 (75.00%), Postives = 15/20 (75.00%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDAC 683
            S PV  H  LMELLIMIDAC
Sbjct:   67 SYPVNLH--LMELLIMIDAC 84
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_STAS1 (Ribose-phosphate pyrophosphokinase OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) GN=prs PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.628e-16
Identity = 33/66 (50.00%), Postives = 51/66 (77.27%), Query Frame = 2 
Query:  443 NRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N   N+ +K+FS   N  L+QE+A ++G+ELGK ++KRF+DGEI + ++ES+RGCDV+++QPT  P
Sbjct:    4 NEYKNSALKIFSLKGNEPLAQEVADHVGIELGKCSVKRFSDGEIQINIEESIRGCDVFIIQPTSNP 69

HSP 2 Score: 26.5646 bits (57), Expect = 1.628e-16
Identity = 15/20 (75.00%), Postives = 15/20 (75.00%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDAC 683
            S PV  H  LMELLIMIDAC
Sbjct:   67 SNPVNLH--LMELLIMIDAC 84
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_STAES (Ribose-phosphate pyrophosphokinase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=prs PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.750e-16
Identity = 34/66 (51.52%), Postives = 51/66 (77.27%), Query Frame = 2 
Query:  443 NRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N   N+ +K+FS   N  L+QE+A ++G+ELGK ++KRF+DGEI + ++ES+RGCDV++VQPT  P
Sbjct:    4 NEYKNSSMKIFSLKGNEPLAQEVADHVGIELGKCSVKRFSDGEIQINIEESIRGCDVFIVQPTSYP 69

HSP 2 Score: 26.1794 bits (56), Expect = 2.750e-16
Identity = 15/20 (75.00%), Postives = 15/20 (75.00%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDAC 683
            S PV  H  LMELLIMIDAC
Sbjct:   67 SYPVNLH--LMELLIMIDAC 84
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_STAEQ (Ribose-phosphate pyrophosphokinase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=prs PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.750e-16
Identity = 34/66 (51.52%), Postives = 51/66 (77.27%), Query Frame = 2 
Query:  443 NRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N   N+ +K+FS   N  L+QE+A ++G+ELGK ++KRF+DGEI + ++ES+RGCDV++VQPT  P
Sbjct:    4 NEYKNSSMKIFSLKGNEPLAQEVADHVGIELGKCSVKRFSDGEIQINIEESIRGCDVFIVQPTSYP 69

HSP 2 Score: 26.1794 bits (56), Expect = 2.750e-16
Identity = 15/20 (75.00%), Postives = 15/20 (75.00%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDAC 683
            S PV  H  LMELLIMIDAC
Sbjct:   67 SYPVNLH--LMELLIMIDAC 84
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_STAHJ (Ribose-phosphate pyrophosphokinase OS=Staphylococcus haemolyticus (strain JCSC1435) GN=prs PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.646e-16
Identity = 33/66 (50.00%), Postives = 50/66 (75.76%), Query Frame = 2 
Query:  443 NRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N   N+ +K+FS   N  L+QE+A  +G+ELGK ++KRF+DGEI + ++ES+RGCDV+++QPT  P
Sbjct:    4 NEYKNSSLKIFSLKGNEPLAQEVADRVGIELGKCSVKRFSDGEIQINIEESIRGCDVFIIQPTSNP 69

HSP 2 Score: 26.5646 bits (57), Expect = 4.646e-16
Identity = 15/20 (75.00%), Postives = 15/20 (75.00%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDAC 683
            S PV  H  LMELLIMIDAC
Sbjct:   67 SNPVNLH--LMELLIMIDAC 84
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_THEMA (Ribose-phosphate pyrophosphokinase OS=Thermotoga maritima GN=prs PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.327e-15
Identity = 32/62 (51.61%), Postives = 48/62 (77.42%), Query Frame = 2 
Query:  455 NNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            +N +K+FSG AN  L+++IA Y+ ++LG   + RFADGEI V++ E+VRG D++L+QPT PP
Sbjct:    4 SNEMKVFSGNANRPLAEKIANYLNLQLGDCEVGRFADGEINVRINETVRGHDIFLIQPTSPP 65

HSP 2 Score: 30.0314 bits (66), Expect = 1.327e-15
Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDA 680
            SPPV  +ENLMELLIMIDA
Sbjct:   63 SPPV--NENLMELLIMIDA 79
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_OCEIH (Ribose-phosphate pyrophosphokinase OS=Oceanobacillus iheyensis GN=prs PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.821e-14
Identity = 32/62 (51.61%), Postives = 47/62 (75.81%), Query Frame = 2 
Query:  455 NNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            ++ +K+FS  +NP L+ EIA ++G  LGK  + +F+DGE+ + L+ESVRGCDVY+VQ TC P
Sbjct:    7 DSSLKVFSLNSNPELANEIATHIGTNLGKCTVSKFSDGEVQINLEESVRGCDVYVVQSTCAP 68

HSP 2 Score: 23.0978 bits (48), Expect = 1.821e-14
Identity = 11/20 (55.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDA 680
            C+P    ++++MELLIMIDA
Sbjct:   66 CAPV---NQHIMELLIMIDA 82
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_DESPS (Ribose-phosphate pyrophosphokinase OS=Desulfotalea psychrophila GN=prs PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.076e-14
Identity = 29/61 (47.54%), Postives = 51/61 (83.61%), Query Frame = 2 
Query:  458 NRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N IK+FSG A+P L+ EIA ++G+++ + ++++F+DGEI+V+++++VRG D ++VQPTC P
Sbjct:    3 NIIKIFSGNAHPQLADEIAAHLGMQVSEADVRKFSDGEIFVEIKKNVRGTDAFIVQPTCTP 63

HSP 2 Score: 23.483 bits (49), Expect = 3.076e-14
Identity = 10/20 (50.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDA 680
            C+P    ++NLMEL++M+DA
Sbjct:   61 CTPV---NDNLMELVLMVDA 77
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_WOLSU (Ribose-phosphate pyrophosphokinase OS=Wolinella succinogenes GN=prs PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.997e-14
Identity = 31/58 (53.45%), Postives = 43/58 (74.14%), Query Frame = 2 
Query:  467 KLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            K+F+G+A+P    EIA Y+G+ L    + RF+DGEI +Q+ ESVRG DV++VQPTC P
Sbjct:    5 KIFTGSAHPEFGGEIAKYLGIPLSSATVNRFSDGEINIQISESVRGRDVFIVQPTCAP 62

HSP 2 Score: 26.1794 bits (56), Expect = 3.997e-14
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDA 680
            C+P    ++NLMELLIMIDA
Sbjct:   60 CAPT---NDNLMELLIMIDA 76
[Close]
BLAST of FC931559 vs. ExPASy Swiss-Prot
Match: KPRS_THEAB (Ribose-phosphate pyrophosphokinase OS=Thermosipho africanus (strain TCF52B) GN=prs PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 6.743e-14
Identity = 30/61 (49.18%), Postives = 44/61 (72.13%), Query Frame = 2 
Query:  458 NRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N +K+FSG AN  L+ +++ Y+G  L    + RFADGEI V++ E+VRG D +++QPTCPP
Sbjct:    6 NEMKIFSGNANRELAIKVSEYIGTRLADCEVGRFADGEINVKIGETVRGHDTFIIQPTCPP 66

HSP 2 Score: 28.4906 bits (62), Expect = 6.743e-14
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 3 
Query:  627 PPVLRHENLMELLIMIDA 680
            PPV  +ENLMELLIMIDA
Sbjct:   65 PPV--NENLMELLIMIDA 80
The following BLAST results are available for this feature:
BLAST of FC931559 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 91
Match NameE-valueIdentityDescription
KPRS_STAAC1.628e-1651.52Ribose-phosphate pyrophosphokinase OS=Staphylococc... [more]
KPRS_STAS11.628e-1650.00Ribose-phosphate pyrophosphokinase OS=Staphylococc... [more]
KPRS_STAES2.750e-1651.52Ribose-phosphate pyrophosphokinase OS=Staphylococc... [more]
KPRS_STAEQ2.750e-1651.52Ribose-phosphate pyrophosphokinase OS=Staphylococc... [more]
KPRS_STAHJ4.646e-1650.00Ribose-phosphate pyrophosphokinase OS=Staphylococc... [more]
KPRS_THEMA1.327e-1551.61Ribose-phosphate pyrophosphokinase OS=Thermotoga m... [more]
KPRS_OCEIH1.821e-1451.61Ribose-phosphate pyrophosphokinase OS=Oceanobacill... [more]
KPRS_DESPS3.076e-1447.54Ribose-phosphate pyrophosphokinase OS=Desulfotalea... [more]
KPRS_WOLSU3.997e-1453.45Ribose-phosphate pyrophosphokinase OS=Wolinella su... [more]
KPRS_THEAB6.743e-1449.18Ribose-phosphate pyrophosphokinase OS=Thermosipho ... [more]

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Properties
Property NameValue
Genbank descriptionC34106E01EF PostHarvP1 Citrus clementina cDNA clone C34106E01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931559 ID=FC931559; Name=FC931559; organism=Citrus clementina; type=EST; length=683bp
GGAAAATCCAAAGCGACGAAGCAAAATAGCCAAAGAAGAAGAAAAAGCAA
CCGAAGCTCTCTCTGCCTCCTCACCGTCACCACAAAAACAAAAGGAAAGA
AAGAAAGAGAGTGAGTGAGAGAAAGAGAGATCAAAAGGAGAGAGAGCTGA
AACAACACAACAGATAACAATGGCTTCGCTGACGATGCCGCGCCCTTCAC
AATCCACGTCAGCTTCTTGCTCTTCGCCTATCCCCAGTCGCTGTTCTTTC
GGTGACCAAAACCCAACTTTTTCATTCAGTTTCAGGGACTCTCGCTCTCG
GGTTCACGTCTGTAATGCCGCCGCCGTTAGATGTGACATGGCTGAGGCAT
TGAATTTTCCAAATGGGAAGCCTAATATTCCAGTTCTAAACGAACGGACG
CTGCCCAAATTTTTGGAAACGGCGCGTATGGAGAAAACTGTCAACAGAAC
TACAAATAACAGGATCAAATTGTTCTCTGGCACCGCCAATCCTGCTCTTT
CCCAGGAAATTGCTTGCTACATGGGCGTGGAACTGGGAAAGATCAACATA
AAGCGATTTGCTGATGGTGAAATTTATGTTCAGCTGCAAGAGAGTGTTAG
AGGATGTGATGTTTATTTGGTGCAGCCCACCTGTCCTCCGGCATGAAAAT
CTCATGGAGCTTTTGATAATGATAGATGCGTGT
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