FC931728

Overview
NameFC931728
Unique NameFC931728
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length736
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: PRPS2_MOUSE (Ribose-phosphate pyrophosphokinase 2 OS=Mus musculus GN=Prps2 PE=1 SV=4)

HSP 1 Score: 58.151 bits (139), Expect = 1.734e-14
Identity = 28/57 (49.12%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:  464 IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTC 634
            I LFSG+++  LSQ +A  +G+ELGK+  K+F++ E  V++ ESVRG DVY++Q  C
Sbjct:    4 IVLFSGSSHQDLSQRVADRLGLELGKVVTKKFSNQETSVEIGESVRGEDVYIIQSGC 60

HSP 2 Score: 41.9726 bits (97), Expect = 1.734e-14
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 3 
Query:  642 HENLMELLIMIDACRRASAKNITAVIPLLGY 734
            ++NLMELLIMI+AC+ AS+  +TAVIP   Y
Sbjct:   64 NDNLMELLIMINACKIASSSRVTAVIPCFPY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: PRPS2_MACFA (Ribose-phosphate pyrophosphokinase 2 OS=Macaca fascicularis GN=PRPS2 PE=2 SV=3)

HSP 1 Score: 58.151 bits (139), Expect = 1.734e-14
Identity = 28/57 (49.12%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:  464 IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTC 634
            I LFSG+++  LSQ +A  +G+ELGK+  K+F++ E  V++ ESVRG DVY++Q  C
Sbjct:    4 IVLFSGSSHQDLSQRVADRLGLELGKVVTKKFSNQETSVEIGESVRGEDVYIIQSGC 60

HSP 2 Score: 41.9726 bits (97), Expect = 1.734e-14
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 3 
Query:  642 HENLMELLIMIDACRRASAKNITAVIPLLGY 734
            ++NLMELLIMI+AC+ AS+  +TAVIP   Y
Sbjct:   64 NDNLMELLIMINACKIASSSRVTAVIPCFPY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: PRPS2_HUMAN (Ribose-phosphate pyrophosphokinase 2 OS=Homo sapiens GN=PRPS2 PE=1 SV=2)

HSP 1 Score: 58.151 bits (139), Expect = 1.734e-14
Identity = 28/57 (49.12%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:  464 IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTC 634
            I LFSG+++  LSQ +A  +G+ELGK+  K+F++ E  V++ ESVRG DVY++Q  C
Sbjct:    4 IVLFSGSSHQDLSQRVADRLGLELGKVVTKKFSNQETSVEIGESVRGEDVYIIQSGC 60

HSP 2 Score: 41.9726 bits (97), Expect = 1.734e-14
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 3 
Query:  642 HENLMELLIMIDACRRASAKNITAVIPLLGY 734
            ++NLMELLIMI+AC+ AS+  +TAVIP   Y
Sbjct:   64 NDNLMELLIMINACKIASSSRVTAVIPCFPY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: PRPS2_CHICK (Ribose-phosphate pyrophosphokinase 2 OS=Gallus gallus GN=PRPS2 PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.252e-14
Identity = 28/57 (49.12%), Postives = 42/57 (73.68%), Query Frame = 2 
Query:  464 IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTC 634
            I LFSG+++  LSQ +A  +G+ELGK+  K+F++ E  V++ ESVRG DVY++Q  C
Sbjct:    4 IVLFSGSSHHDLSQRVADRLGLELGKVVTKKFSNQETSVEIGESVRGEDVYIIQSGC 60

HSP 2 Score: 41.9726 bits (97), Expect = 2.252e-14
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 3 
Query:  642 HENLMELLIMIDACRRASAKNITAVIPLLGY 734
            ++NLMELLIMI+AC+ AS+  +TAVIP   Y
Sbjct:   64 NDNLMELLIMINACKIASSSRVTAVIPCFPY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_CLOAB (Ribose-phosphate pyrophosphokinase OS=Clostridium acetobutylicum GN=prs PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.253e-14
Identity = 25/59 (42.37%), Postives = 43/59 (72.88%), Query Frame = 2 
Query:  464 IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            IK+F+G + P+L++EIA  +GV++G   + +F++GE  V + E+VRG D++L+Q  C P
Sbjct:    8 IKIFTGNSYPSLAEEIADIIGVQVGDSRVGKFSNGETAVDINETVRGTDLFLIQTLCEP 66

HSP 2 Score: 39.6614 bits (91), Expect = 2.253e-14
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 3 
Query:  642 HENLMELLIMIDACRRASAKNITAVIPLLGY 734
            ++++MELLIM+DA +RASA  ITAVIP   Y
Sbjct:   68 NDSIMELLIMLDAFKRASAGRITAVIPNYAY 98
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_RHOSK (Ribose-phosphate pyrophosphokinase OS=Rhodobacter sphaeroides (strain KD131 / KCTC 12085) GN=prs PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.081e-13
Identity = 30/68 (44.12%), Postives = 41/68 (60.29%), Query Frame = 2 
Query:  458 NRIKLFSGTANPALSQEIACYMGVELGKI------NIKRFADGEIYVQLQESVRGCDVYLVQPTCPPA 643
            N  KL SG AN  L++ IA  M V  G         ++RF D EI+V++ E+VRG D+Y++QPT  PA
Sbjct:    2 NEPKLISGNANLTLAKSIARRMSVHRGMAVSLVDARVERFNDQEIFVEVYENVRGEDMYVIQPTSNPA 69

HSP 2 Score: 43.8986 bits (102), Expect = 1.081e-13
Identity = 21/31 (67.74%), Postives = 25/31 (80.65%), Query Frame = 3 
Query:  642 HENLMELLIMIDACRRASAKNITAVIPLLGY 734
            ++NLMELLI+ DA RR+SA  ITAVIP  GY
Sbjct:   70 NDNLMELLIIADALRRSSADRITAVIPYFGY 100
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_AQUAE (Ribose-phosphate pyrophosphokinase OS=Aquifex aeolicus GN=prs PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.084e-13
Identity = 28/61 (45.90%), Postives = 40/61 (65.57%), Query Frame = 2 
Query:  458 NRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N +K+ +GT+N  LS+EIA Y+G+ L    I  F+DGEI VQ+ ES+RG  VY++     P
Sbjct:    2 NGVKIIAGTSNKTLSEEIAGYLGLRLTDALITTFSDGEIRVQINESIRGYHVYVITSLSNP 62

HSP 2 Score: 38.891 bits (89), Expect = 1.084e-13
Identity = 17/31 (54.84%), Postives = 23/31 (74.19%), Query Frame = 3 
Query:  642 HENLMELLIMIDACRRASAKNITAVIPLLGY 734
            + NLMELL+ +DA +R+S K ITAV+P   Y
Sbjct:   64 NHNLMELLLTLDAVKRSSPKEITAVVPYYAY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS1_STRA5 (Ribose-phosphate pyrophosphokinase 1 OS=Streptococcus agalactiae serotype V GN=prs1 PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.407e-13
Identity = 22/61 (36.07%), Postives = 45/61 (73.77%), Query Frame = 2 
Query:  458 NRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            + +KLF+ ++N  L+++++  +G+ LG+  +++F+DGEI V ++ES+RG  V+++Q T  P
Sbjct:    4 SNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQVNIEESIRGHHVFILQSTSSP 64

HSP 2 Score: 42.743 bits (99), Expect = 1.407e-13
Identity = 20/37 (54.05%), Postives = 30/37 (81.08%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            S PV  ++NLME+LIM+DA +RASA++++ V+P  GY
Sbjct:   62 SSPV--NDNLMEILIMVDALKRASAESVSVVMPYYGY 96
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS1_STRA3 (Ribose-phosphate pyrophosphokinase 1 OS=Streptococcus agalactiae serotype III GN=prs1 PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.407e-13
Identity = 22/61 (36.07%), Postives = 45/61 (73.77%), Query Frame = 2 
Query:  458 NRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            + +KLF+ ++N  L+++++  +G+ LG+  +++F+DGEI V ++ES+RG  V+++Q T  P
Sbjct:    4 SNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQVNIEESIRGHHVFILQSTSSP 64

HSP 2 Score: 42.743 bits (99), Expect = 1.407e-13
Identity = 20/37 (54.05%), Postives = 30/37 (81.08%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            S PV  ++NLME+LIM+DA +RASA++++ V+P  GY
Sbjct:   62 SSPV--NDNLMEILIMVDALKRASAESVSVVMPYYGY 96
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_MYCTU (Ribose-phosphate pyrophosphokinase OS=Mycobacterium tuberculosis GN=prs PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 2.373e-13
Identity = 27/64 (42.19%), Postives = 46/64 (71.88%), Query Frame = 2 
Query:  452 TNNR--IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCP 637
            T+NR  + LF+G A+P L++++A  + V +   + + FA+GEI+V+  ESVRGCD +++Q +CP
Sbjct:    6 TDNRKNLMLFAGRAHPELAEQVAKELDVHVTSQDAREFANGEIFVRFHESVRGCDAFVLQ-SCP 68

HSP 2 Score: 40.0466 bits (92), Expect = 2.373e-13
Identity = 23/38 (60.53%), Postives = 25/38 (65.79%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            C  PV R   LME LIMIDA +R SAK ITAV+P   Y
Sbjct:   67 CPAPVNRW--LMEQLIMIDALKRGSAKRITAVMPFYPY 102
The following BLAST results are available for this feature:
BLAST of FC931728 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 146
Match NameE-valueIdentityDescription
PRPS2_MOUSE1.734e-1449.12Ribose-phosphate pyrophosphokinase 2 OS=Mus muscul... [more]
PRPS2_MACFA1.734e-1449.12Ribose-phosphate pyrophosphokinase 2 OS=Macaca fas... [more]
PRPS2_HUMAN1.734e-1449.12Ribose-phosphate pyrophosphokinase 2 OS=Homo sapie... [more]
PRPS2_CHICK2.252e-1449.12Ribose-phosphate pyrophosphokinase 2 OS=Gallus gal... [more]
KPRS_CLOAB2.253e-1442.37Ribose-phosphate pyrophosphokinase OS=Clostridium ... [more]
KPRS_RHOSK1.081e-1344.12Ribose-phosphate pyrophosphokinase OS=Rhodobacter ... [more]
KPRS_AQUAE1.084e-1345.90Ribose-phosphate pyrophosphokinase OS=Aquifex aeol... [more]
KPRS1_STRA51.407e-1336.07Ribose-phosphate pyrophosphokinase 1 OS=Streptococ... [more]
KPRS1_STRA31.407e-1336.07Ribose-phosphate pyrophosphokinase 1 OS=Streptococ... [more]
KPRS_MYCTU2.373e-1342.19Ribose-phosphate pyrophosphokinase OS=Mycobacteriu... [more]

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Properties
Property NameValue
Genbank descriptionC34108C11EF PostHarvP1 Citrus clementina cDNA clone C34108C11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931728 ID=FC931728; Name=FC931728; organism=Citrus clementina; type=EST; length=736bp
GGAAAATCCAAAGCGACGAAGCAAAATAGCCAAAGAAGAAGAAAAAGCAA
CCGAAGCTCTCTCTGCCTCCTCACCGTCACCACAAAAACAAAAGGAAAGA
AAGAAAGAGAGTGAGTGAGAGAAAGAGAGATCAAAAGGAGAGAGAGCTGA
AACAACACAACAGATAACAATGGCTTCGCTGACGATGCCGCGCCCTTCAC
AATCCACGTCAGCTTCTTGCTCTTCGCCTATCCCCAGTCGCTGTTCTTTC
GGTGACCAAAACCCAACTTTTTCATTCAGTTTCAGGGACTCTCGCTCTCG
GGTTCACGTCTGTAATGCCGCCGCCGTTAGATGTGACATGGCTGAGGCAT
TGAATTTTCCAAATGGGAAGCCTAATATTCCAGTTCTAAACGAACGGACG
CTGCCCAAATTTTTGGAAACGGCGCGTATGGAGAAAACTGTCAACAGAAC
TACAAATAACAGGATCAAATTGTTCTCTGGCACCGCCAATCCTGCTCTTT
CCCAGGAAATTGCTTGCTACATGGGCGTGGAACTGGGAAAGATCAACATA
AAGCGATTTGCTGATGGTGAAATTTATGTTCAGCTGCAAGAGAGTGTTAG
AGGATGTGATGTTTATTTGGTGCAGCCCACCTGTCCTCCGGCATGAAAAT
CTCATGGAGCTTTTGATAATGATAGATGCGTGTCGGAGAGCGTCAGCCAA
GAATATCACAGCTGTGATTCCTTTACTTGGATATGC
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