FC931728

Overview
NameFC931728
Unique NameFC931728
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length736
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_BACSU (Ribose-phosphate pyrophosphokinase OS=Bacillus subtilis GN=prs PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.682e-19
Identity = 30/66 (45.45%), Postives = 49/66 (74.24%), Query Frame = 2 
Query:  443 NRTTNNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N+  +  +K+FS  +NP L++EIA  +GV+LGK ++ RF+DGE+ + ++ES+RGCD Y++Q T  P
Sbjct:    3 NQYGDKNLKIFSLNSNPELAKEIADIVGVQLGKCSVTRFSDGEVQINIEESIRGCDCYIIQSTSDP 68

HSP 2 Score: 43.8986 bits (102), Expect = 1.682e-19
Identity = 23/37 (62.16%), Postives = 28/37 (75.68%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            S PV  +E++MELLIM+DA +RASAK I  VIP  GY
Sbjct:   66 SDPV--NEHIMELLIMVDALKRASAKTINIVIPYYGY 100
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_THEAB (Ribose-phosphate pyrophosphokinase OS=Thermosipho africanus (strain TCF52B) GN=prs PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.188e-19
Identity = 30/61 (49.18%), Postives = 44/61 (72.13%), Query Frame = 2 
Query:  458 NRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N +K+FSG AN  L+ +++ Y+G  L    + RFADGEI V++ E+VRG D +++QPTCPP
Sbjct:    6 NEMKIFSGNANRELAIKVSEYIGTRLADCEVGRFADGEINVKIGETVRGHDTFIIQPTCPP 66

HSP 2 Score: 47.3654 bits (111), Expect = 2.188e-19
Identity = 25/36 (69.44%), Postives = 28/36 (77.78%), Query Frame = 3 
Query:  627 PPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            PPV  +ENLMELLIMIDA +RASA +I  VIP  GY
Sbjct:   65 PPV--NENLMELLIMIDALKRASANSIAVVIPYYGY 98
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_HELPY (Ribose-phosphate pyrophosphokinase OS=Helicobacter pylori GN=prs PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.845e-19
Identity = 29/58 (50.00%), Postives = 45/58 (77.59%), Query Frame = 2 
Query:  467 KLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            K+FSG+A+PA  +E++ ++G  L K  I +F+DGEI +Q+ ESVRG D++++QPTC P
Sbjct:   14 KIFSGSAHPAFGKEVSKHLGFPLSKAVIGKFSDGEINIQISESVRGKDIFIIQPTCVP 71

HSP 2 Score: 46.595 bits (109), Expect = 2.845e-19
Identity = 20/31 (64.52%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  642 HENLMELLIMIDACRRASAKNITAVIPLLGY 734
            ++NLMELL+M+DA RR+SA +ITAV+P  GY
Sbjct:   73 NDNLMELLVMVDALRRSSANSITAVLPYFGY 103
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_CLOPE (Ribose-phosphate pyrophosphokinase OS=Clostridium perfringens GN=prs PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.811e-19
Identity = 30/62 (48.39%), Postives = 46/62 (74.19%), Query Frame = 2 
Query:  455 NNRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            +  IK+F+G ++P L++EIA  + + LGK  +  F+DGEI V ++E+VRGCDV++VQ TC P
Sbjct:    5 SKNIKIFTGNSHPELAREIAKALNIPLGKAEVGTFSDGEISVNIKETVRGCDVFIVQSTCSP 66

HSP 2 Score: 45.0542 bits (105), Expect = 4.811e-19
Identity = 25/38 (65.79%), Postives = 27/38 (71.05%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            CSP    + NLMELLIMIDA +RASA  I AVIP  GY
Sbjct:   64 CSPV---NNNLMELLIMIDAFKRASAGRINAVIPYYGY 98
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_VIBPA (Ribose-phosphate pyrophosphokinase OS=Vibrio parahaemolyticus GN=prs PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.140e-19
Identity = 29/59 (49.15%), Postives = 43/59 (72.88%), Query Frame = 2 
Query:  464 IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            +KLF+G A P L+Q IA  + + LG  ++ RF+DGE+ VQ+ E+VRG DV+++Q TC P
Sbjct:    4 MKLFAGNATPELAQRIADRLYISLGDASVSRFSDGEVAVQINENVRGSDVFIIQSTCAP 62

HSP 2 Score: 47.3654 bits (111), Expect = 8.140e-19
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            C+P    ++NLMEL++MIDA RRASA  ITAVIP  GY
Sbjct:   60 CAPT---NDNLMELVVMIDAMRRASAGRITAVIPYFGY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_VIBVY (Ribose-phosphate pyrophosphokinase OS=Vibrio vulnificus (strain YJ016) GN=prs PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.059e-18
Identity = 29/59 (49.15%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  464 IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            +KLF+G A P L+Q IA  + + LG   + RF+DGE+ VQ+ E+VRG DV+++Q TC P
Sbjct:    4 MKLFAGNATPELAQRIADRLYISLGDATVSRFSDGEVAVQINENVRGSDVFIIQSTCAP 62

HSP 2 Score: 47.3654 bits (111), Expect = 1.059e-18
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            C+P    ++NLMEL++MIDA RRASA  ITAVIP  GY
Sbjct:   60 CAPT---NDNLMELVVMIDAMRRASAGRITAVIPYFGY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_VIBVU (Ribose-phosphate pyrophosphokinase OS=Vibrio vulnificus GN=prs PE=3 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 1.059e-18
Identity = 29/59 (49.15%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  464 IKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            +KLF+G A P L+Q IA  + + LG   + RF+DGE+ VQ+ E+VRG DV+++Q TC P
Sbjct:    4 MKLFAGNATPELAQRIADRLYISLGDATVSRFSDGEVAVQINENVRGSDVFIIQSTCAP 62

HSP 2 Score: 47.3654 bits (111), Expect = 1.059e-18
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            C+P    ++NLMEL++MIDA RRASA  ITAVIP  GY
Sbjct:   60 CAPT---NDNLMELVVMIDAMRRASAGRITAVIPYFGY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_FUSNN (Ribose-phosphate pyrophosphokinase OS=Fusobacterium nucleatum subsp. nucleatum GN=prs PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.790e-18
Identity = 30/61 (49.18%), Postives = 47/61 (77.05%), Query Frame = 2 
Query:  458 NRIKLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPP 640
            N +K+FSG +N  L+++IA   G++LGK  I+RF DGE+Y++++E+VRG DV++VQ T  P
Sbjct:    5 NNVKIFSGNSNLELAKKIAEKAGLQLGKAEIQRFKDGEVYIEIEETVRGRDVFVVQSTSEP 65

HSP 2 Score: 44.669 bits (104), Expect = 1.790e-18
Identity = 23/37 (62.16%), Postives = 27/37 (72.97%), Query Frame = 3 
Query:  624 SPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            S PV  +ENLMELLI +DA +RASAK I  +IP  GY
Sbjct:   63 SEPV--NENLMELLIFVDALKRASAKTINVIIPYYGY 97
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_HELHP (Ribose-phosphate pyrophosphokinase OS=Helicobacter hepaticus GN=prs PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.790e-18
Identity = 31/59 (52.54%), Postives = 43/59 (72.88%), Query Frame = 2 
Query:  467 KLFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPPA 643
            K+FSG+A+   S E+A  + + L K  I RF+DGEI VQ+ ESVRG D++++QPTC PA
Sbjct:    5 KIFSGSAHTLFSNEVAKCLDISLSKTTINRFSDGEINVQIGESVRGKDIFIIQPTCAPA 63

HSP 2 Score: 44.2838 bits (103), Expect = 1.790e-18
Identity = 22/38 (57.89%), Postives = 28/38 (73.68%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            C+P    +++LMELLIM DA +R+ AKNI AVIP  GY
Sbjct:   60 CAPA---NDHLMELLIMTDALKRSGAKNINAVIPYFGY 94
[Close]
BLAST of FC931728 vs. ExPASy Swiss-Prot
Match: KPRS_XANAC (Ribose-phosphate pyrophosphokinase OS=Xanthomonas axonopodis pv. citri GN=prs PE=3 SV=2)

HSP 1 Score: 65.0846 bits (157), Expect = 2.326e-18
Identity = 28/58 (48.28%), Postives = 43/58 (74.14%), Query Frame = 2 
Query:  470 LFSGTANPALSQEIACYMGVELGKINIKRFADGEIYVQLQESVRGCDVYLVQPTCPPA 643
            +FSG AN  L+Q I   +GV +GK  + RF+DGE+ V+++ESVR  +V+++QPTC P+
Sbjct:    9 VFSGNANKPLAQSICKELGVRMGKALVTRFSDGEVQVEIEESVRRQEVFVIQPTCAPS 66

HSP 2 Score: 48.1358 bits (113), Expect = 2.326e-18
Identity = 23/38 (60.53%), Postives = 30/38 (78.95%), Query Frame = 3 
Query:  621 CSPPVLRHENLMELLIMIDACRRASAKNITAVIPLLGY 734
            C+P     ENLMELL++IDA +RASA+++TAVIP  GY
Sbjct:   63 CAPSA---ENLMELLVLIDALKRASAQSVTAVIPYFGY 97
The following BLAST results are available for this feature:
BLAST of FC931728 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 146
Match NameE-valueIdentityDescription
KPRS_BACSU1.682e-1945.45Ribose-phosphate pyrophosphokinase OS=Bacillus sub... [more]
KPRS_THEAB2.188e-1949.18Ribose-phosphate pyrophosphokinase OS=Thermosipho ... [more]
KPRS_HELPY2.845e-1950.00Ribose-phosphate pyrophosphokinase OS=Helicobacter... [more]
KPRS_CLOPE4.811e-1948.39Ribose-phosphate pyrophosphokinase OS=Clostridium ... [more]
KPRS_VIBPA8.140e-1949.15Ribose-phosphate pyrophosphokinase OS=Vibrio parah... [more]
KPRS_VIBVY1.059e-1849.15Ribose-phosphate pyrophosphokinase OS=Vibrio vulni... [more]
KPRS_VIBVU1.059e-1849.15Ribose-phosphate pyrophosphokinase OS=Vibrio vulni... [more]
KPRS_FUSNN1.790e-1849.18Ribose-phosphate pyrophosphokinase OS=Fusobacteriu... [more]
KPRS_HELHP1.790e-1852.54Ribose-phosphate pyrophosphokinase OS=Helicobacter... [more]
KPRS_XANAC2.326e-1848.28Ribose-phosphate pyrophosphokinase OS=Xanthomonas ... [more]

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Properties
Property NameValue
Genbank descriptionC34108C11EF PostHarvP1 Citrus clementina cDNA clone C34108C11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931728 ID=FC931728; Name=FC931728; organism=Citrus clementina; type=EST; length=736bp
GGAAAATCCAAAGCGACGAAGCAAAATAGCCAAAGAAGAAGAAAAAGCAA
CCGAAGCTCTCTCTGCCTCCTCACCGTCACCACAAAAACAAAAGGAAAGA
AAGAAAGAGAGTGAGTGAGAGAAAGAGAGATCAAAAGGAGAGAGAGCTGA
AACAACACAACAGATAACAATGGCTTCGCTGACGATGCCGCGCCCTTCAC
AATCCACGTCAGCTTCTTGCTCTTCGCCTATCCCCAGTCGCTGTTCTTTC
GGTGACCAAAACCCAACTTTTTCATTCAGTTTCAGGGACTCTCGCTCTCG
GGTTCACGTCTGTAATGCCGCCGCCGTTAGATGTGACATGGCTGAGGCAT
TGAATTTTCCAAATGGGAAGCCTAATATTCCAGTTCTAAACGAACGGACG
CTGCCCAAATTTTTGGAAACGGCGCGTATGGAGAAAACTGTCAACAGAAC
TACAAATAACAGGATCAAATTGTTCTCTGGCACCGCCAATCCTGCTCTTT
CCCAGGAAATTGCTTGCTACATGGGCGTGGAACTGGGAAAGATCAACATA
AAGCGATTTGCTGATGGTGAAATTTATGTTCAGCTGCAAGAGAGTGTTAG
AGGATGTGATGTTTATTTGGTGCAGCCCACCTGTCCTCCGGCATGAAAAT
CTCATGGAGCTTTTGATAATGATAGATGCGTGTCGGAGAGCGTCAGCCAA
GAATATCACAGCTGTGATTCCTTTACTTGGATATGC
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