FC931771

Overview
NameFC931771
Unique NameFC931771
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length672
Libraries
Library NameType
PostHarveNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig15209 contig Ccv1_Contig15209:96..768. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL101_ARATH (60S ribosomal protein L10-1 OS=Arabidopsis thaliana GN=RPL10A PE=2 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 1.250e-85
Identity = 157/205 (76.59%), Postives = 167/205 (81.46%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQKSLSAESGVSPSLAAVII*GSSQRTVLCPMVLNASFLDVMDP 668
            MGRRPARCYRQIK KPYPKSR+CRGVPDPKIRIYDVGMK+KGVDEFPFCVHLVSWEKE+VSSEALEAARIACNKYM K AGKDAFHLR+RVHPFHVLRINKMLSCAGADRLQTGMRGAFGK  G CARVAIGQVLLSVRCKD++  HAQEALRRAKFKFPGRQK + +                 Q   + P  +NA FL    P
Sbjct:    1 MGRRPARCYRQIKGKPYPKSRYCRGVPDPKIRIYDVGMKRKGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMVKSAGKDAFHLRIRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKALGTCARVAIGQVLLSVRCKDAHGHHAQEALRRAKFKFPGRQKIIVSRKWGFTKFNRADFTKLRQEKRVVPDGVNAKFLSCHGP 205

HSP 2 Score: 70.8626 bits (172), Expect = 7.003e-12
Identity = 29/43 (67.44%), Postives = 35/43 (81.40%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KIIVSRKWGFTKF+R+D+ + + E R+VPDGV  K L CHGPL
Sbjct:  164 KIIVSRKWGFTKFNRADFTKLRQEKRVVPDGVNAKFLSCHGPL 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL102_ARATH (60S ribosomal protein L10-2 OS=Arabidopsis thaliana GN=RPL10B PE=2 SV=2)

HSP 1 Score: 314.694 bits (805), Expect = 2.784e-85
Identity = 156/205 (76.10%), Postives = 167/205 (81.46%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQKSLSAESGVSPSLAAVII*GSSQRTVLCPMVLNASFLDVMDP 668
            MGRRPARCYRQIK KPYPKSR+CRGVPDPKIRIYDVGMK+KGVDEFP+CVHLVSWEKE+VSSEALEAARIACNKYM K AGKDAFHLR+RVHPFHVLRINKMLSCAGADRLQTGMRGAFGK  G CARVAIGQVLLSVRCKD++  HAQEALRRAKFKFPGRQK + +                 Q   + P  +NA FL    P
Sbjct:    1 MGRRPARCYRQIKGKPYPKSRYCRGVPDPKIRIYDVGMKRKGVDEFPYCVHLVSWEKENVSSEALEAARIACNKYMVKSAGKDAFHLRIRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKALGTCARVAIGQVLLSVRCKDAHGHHAQEALRRAKFKFPGRQKIIVSRKWGFTKFNRADYTKLRQEKRIVPDGVNAKFLSCHGP 205

HSP 2 Score: 72.7886 bits (177), Expect = 1.843e-12
Identity = 31/43 (72.09%), Postives = 35/43 (81.40%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KIIVSRKWGFTKF+R+DY + + E RIVPDGV  K L CHGPL
Sbjct:  164 KIIVSRKWGFTKFNRADYTKLRQEKRIVPDGVNAKFLSCHGPL 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL10_DROME (60S ribosomal protein L10 OS=Drosophila melanogaster GN=Qm PE=2 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 9.665e-78
Identity = 124/164 (75.61%), Postives = 137/164 (83.54%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQK 545
            MGRRPARCYR  KNKPYPKSRFCRGVPDPKIRI+D+G KK  V++FP CVHLVS E E +SSEALEA RI CNKY+ K+ GKD FH+R+R+HPFHV+RINKMLSCAGADRLQTGMRGAFGKPQG  ARV IGQ ++SVR  D       EALRRAKFKFPGRQK
Sbjct:    1 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGRKKATVEDFPLCVHLVSDEYEQLSSEALEAGRICCNKYLVKYCGKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPIMSVRSSDRYKAQVIEALRRAKFKFPGRQK 164

HSP 2 Score: 49.6766 bits (117), Expect = 9.665e-78
Identity = 21/43 (48.84%), Postives = 27/43 (62.79%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KI VS+KWGFTK+ R  Y   + +NR+ PDG   K    HGP+
Sbjct:  164 KIYVSKKWGFTKYERERYEELRDDNRLEPDGCNVKYRPEHGPI 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL10_SHEEP (60S ribosomal protein L10 OS=Ovis aries GN=RPL10 PE=2 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 8.096e-77
Identity = 127/164 (77.44%), Postives = 137/164 (83.54%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQK 545
            MGRRPARCYR  KNKPYPKSRFCRGVPD KIRI+D+G KK  VDEFP C H+VS E E +SSEALEAARI  NKYM K  GKD FH+RVR+HPFHV+RINKMLSCAGADRLQTGMRGAFGKPQG  ARV IGQV++S+R K  N  H  EALRRAKFKFPGRQK
Sbjct:    1 MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQK 164

HSP 2 Score: 46.2098 bits (108), Expect = 8.096e-77
Identity = 19/43 (44.19%), Postives = 28/43 (65.12%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KI +S+KWGFTKF+  ++    +E R++PDG   K +   GPL
Sbjct:  164 KIHISKKWGFTKFNADEFENMVAEKRLIPDGCGVKYIPNRGPL 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL10_PIG (60S ribosomal protein L10 OS=Sus scrofa GN=RPL10 PE=2 SV=3)

HSP 1 Score: 262.692 bits (670), Expect = 8.096e-77
Identity = 127/164 (77.44%), Postives = 137/164 (83.54%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQK 545
            MGRRPARCYR  KNKPYPKSRFCRGVPD KIRI+D+G KK  VDEFP C H+VS E E +SSEALEAARI  NKYM K  GKD FH+RVR+HPFHV+RINKMLSCAGADRLQTGMRGAFGKPQG  ARV IGQV++S+R K  N  H  EALRRAKFKFPGRQK
Sbjct:    1 MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQK 164

HSP 2 Score: 46.2098 bits (108), Expect = 8.096e-77
Identity = 19/43 (44.19%), Postives = 28/43 (65.12%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KI +S+KWGFTKF+  ++    +E R++PDG   K +   GPL
Sbjct:  164 KIHISKKWGFTKFNADEFENMVAEKRLIPDGCGVKYIPNRGPL 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL10_BOVIN (60S ribosomal protein L10 OS=Bos taurus GN=RPL10 PE=2 SV=4)

HSP 1 Score: 262.692 bits (670), Expect = 8.096e-77
Identity = 127/164 (77.44%), Postives = 137/164 (83.54%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQK 545
            MGRRPARCYR  KNKPYPKSRFCRGVPD KIRI+D+G KK  VDEFP C H+VS E E +SSEALEAARI  NKYM K  GKD FH+RVR+HPFHV+RINKMLSCAGADRLQTGMRGAFGKPQG  ARV IGQV++S+R K  N  H  EALRRAKFKFPGRQK
Sbjct:    1 MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQK 164

HSP 2 Score: 46.2098 bits (108), Expect = 8.096e-77
Identity = 19/43 (44.19%), Postives = 28/43 (65.12%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KI +S+KWGFTKF+  ++    +E R++PDG   K +   GPL
Sbjct:  164 KIHISKKWGFTKFNADEFENMVAEKRLIPDGCGVKYIPNRGPL 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL10L_MACFA (60S ribosomal protein L10-like OS=Macaca fascicularis GN=RPL10L PE=2 SV=1)

HSP 1 Score: 261.536 bits (667), Expect = 8.096e-77
Identity = 126/164 (76.83%), Postives = 137/164 (83.54%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQK 545
            MGRRPARCYR  KNKPYPKSRFCRGVPD KIRI+D+G KK  VDEFP C H+VS E E +SSEALEAARI  NKYM K  G+D FH+RVR+HPFHV+RINKMLSCAGADRLQTGMRGAFGKPQG  ARV IGQV++S+R K  N  H  EALRRAKFKFPGRQK
Sbjct:    1 MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGRDGFHMRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQK 164

HSP 2 Score: 47.3654 bits (111), Expect = 8.096e-77
Identity = 19/43 (44.19%), Postives = 29/43 (67.44%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KI +S+KWGFTKF+  ++    ++ R++PDG   K +  HGPL
Sbjct:  164 KIHISKKWGFTKFNADEFEDMVAKKRLIPDGCGVKYVPSHGPL 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL10_RAT (60S ribosomal protein L10 OS=Rattus norvegicus GN=Rpl10 PE=1 SV=3)

HSP 1 Score: 262.692 bits (670), Expect = 1.377e-76
Identity = 127/164 (77.44%), Postives = 137/164 (83.54%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQK 545
            MGRRPARCYR  KNKPYPKSRFCRGVPD KIRI+D+G KK  VDEFP C H+VS E E +SSEALEAARI  NKYM K  GKD FH+RVR+HPFHV+RINKMLSCAGADRLQTGMRGAFGKPQG  ARV IGQV++S+R K  N  H  EALRRAKFKFPGRQK
Sbjct:    1 MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQK 164

HSP 2 Score: 45.4394 bits (106), Expect = 1.377e-76
Identity = 19/43 (44.19%), Postives = 28/43 (65.12%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KI +S+KWGFTKF+  ++    +E R++PDG   K +   GPL
Sbjct:  164 KIHISKKWGFTKFNADEFEDMVAEKRLIPDGCGVKYIPNRGPL 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL10_PAPAN (60S ribosomal protein L10 OS=Papio anubis GN=RPL10 PE=3 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 1.377e-76
Identity = 127/164 (77.44%), Postives = 137/164 (83.54%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQK 545
            MGRRPARCYR  KNKPYPKSRFCRGVPD KIRI+D+G KK  VDEFP C H+VS E E +SSEALEAARI  NKYM K  GKD FH+RVR+HPFHV+RINKMLSCAGADRLQTGMRGAFGKPQG  ARV IGQV++S+R K  N  H  EALRRAKFKFPGRQK
Sbjct:    1 MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQK 164

HSP 2 Score: 45.4394 bits (106), Expect = 1.377e-76
Identity = 19/43 (44.19%), Postives = 28/43 (65.12%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KI +S+KWGFTKF+  ++    +E R++PDG   K +   GPL
Sbjct:  164 KIHISKKWGFTKFNADEFEDMVAEKRLIPDGCGVKYIPNRGPL 206
[Close]
BLAST of FC931771 vs. ExPASy Swiss-Prot
Match: RL10_MOUSE (60S ribosomal protein L10 OS=Mus musculus GN=Rpl10 PE=2 SV=3)

HSP 1 Score: 262.692 bits (670), Expect = 1.377e-76
Identity = 127/164 (77.44%), Postives = 137/164 (83.54%), Query Frame = 3 
Query:   54 MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKESVSSEALEAARIACNKYMAKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGACARVAIGQVLLSVRCKDSNSLHAQEALRRAKFKFPGRQK 545
            MGRRPARCYR  KNKPYPKSRFCRGVPD KIRI+D+G KK  VDEFP C H+VS E E +SSEALEAARI  NKYM K  GKD FH+RVR+HPFHV+RINKMLSCAGADRLQTGMRGAFGKPQG  ARV IGQV++S+R K  N  H  EALRRAKFKFPGRQK
Sbjct:    1 MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQK 164

HSP 2 Score: 45.4394 bits (106), Expect = 1.377e-76
Identity = 19/43 (44.19%), Postives = 28/43 (65.12%), Query Frame = 2 
Query:  542 KIIVSRKWGFTKFSRSDYLRFKSENRIVPDGVKCKLLGCHGPL 670
            KI +S+KWGFTKF+  ++    +E R++PDG   K +   GPL
Sbjct:  164 KIHISKKWGFTKFNADEFEDMVAEKRLIPDGCGVKYIPNRGPL 206
The following BLAST results are available for this feature:
BLAST of FC931771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 92
Match NameE-valueIdentityDescription
RL101_ARATH1.250e-8576.5960S ribosomal protein L10-1 OS=Arabidopsis thalian... [more]
RL102_ARATH2.784e-8576.1060S ribosomal protein L10-2 OS=Arabidopsis thalian... [more]
RL10_DROME9.665e-7875.6160S ribosomal protein L10 OS=Drosophila melanogast... [more]
RL10_SHEEP8.096e-7777.4460S ribosomal protein L10 OS=Ovis aries GN=RPL10 P... [more]
RL10_PIG8.096e-7777.4460S ribosomal protein L10 OS=Sus scrofa GN=RPL10 P... [more]
RL10_BOVIN8.096e-7777.4460S ribosomal protein L10 OS=Bos taurus GN=RPL10 P... [more]
RL10L_MACFA8.096e-7776.8360S ribosomal protein L10-like OS=Macaca fascicula... [more]
RL10_RAT1.377e-7677.4460S ribosomal protein L10 OS=Rattus norvegicus GN=... [more]
RL10_PAPAN1.377e-7677.4460S ribosomal protein L10 OS=Papio anubis GN=RPL10... [more]
RL10_MOUSE1.377e-7677.4460S ribosomal protein L10 OS=Mus musculus GN=Rpl10... [more]

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Properties
Property NameValue
Genbank descriptionC34201A07EF PostHarveN Citrus clementina cDNA clone C34201A07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931771 ID=FC931771; Name=FC931771; organism=Citrus clementina; type=EST; length=672bp
GACTTTTCTTGGGTTTCTGGTTTAGTTGCTGAATACCTACCAGGGCTTTC
GCCATGGGAAGAAGACCTGCTAGGTGTTATCGCCAGATAAAAAATAAGCC
GTATCCGAAATCACGCTTTTGTCGTGGTGTGCCTGATCCCAAGATCAGGA
TCTATGATGTTGGAATGAAGAAGAAAGGGGTTGATGAATTCCCTTTCTGT
GTGCACTTGGTGTCTTGGGAAAAGGAGAGCGTATCTAGTGAAGCTCTTGA
GGCTGCTCGAATTGCTTGCAACAAATATATGGCCAAGTTTGCCGGGAAAG
ATGCTTTCCATTTGCGAGTCCGTGTTCACCCATTCCATGTCTTGCGTATC
AACAAAATGCTTTCATGTGCTGGAGCTGATAGGCTTCAAACTGGTATGAG
GGGTGCTTTCGGTAAACCACAGGGCGCTTGTGCTAGAGTTGCCATTGGAC
AGGTCCTCCTGTCAGTTCGCTGCAAGGATAGCAATAGTCTTCATGCTCAA
GAGGCTCTTCGTCGTGCAAAGTTCAAGTTTCCTGGTCGCCAAAAATCATT
GTCAGCAGAAAGTGGGGTTTCACCAAGTTTAGCCGCAGTGATTATTTGAG
GTTCAAGTCAGAGAACCGTATTGTGCCCGATGGTGTTAAATGCAAGCTTC
TTGGATGTCATGGACCCTTGGC
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