FC931933

Overview
NameFC931933
Unique NameFC931933
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length712
Libraries
Library NameType
PostHarveNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_THEON (Elongation factor 2 OS=Thermococcus onnurineus (strain NA1) GN=fusA PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.247e-33
Identity = 50/130 (38.46%), Postives = 73/130 (56.15%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRI--GPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPLL 687
            +HNR Y+   PL + + EAI +G I  G   DPK  +K L+E  G D ++AK I     +    N+ +D  KG+QYLNE+ D +V GF  A  EG LA+E +  +   + D  +H D +HRG  Q+ P +
Sbjct:  483 KHNRFYITVEPLPDAIYEAIREGEIPEGRPKDPKAVAKKLAE-LGLDYEIAKGI----VDVYNGNIFLDNTKGIQYLNEVMDLLVDGFHQAMDEGPLAKEPVMKVMVRLHDAKIHEDNVHRGPAQIYPAI 607          

HSP 2 Score: 77.411 bits (189), Expect = 1.247e-33
Identity = 43/101 (42.57%), Postives = 67/101 (66.34%), Query Frame = 2
Query:    5 FSVSPVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            ++  PVV VA++ K   DLPKL+E L++LAK DP +   + EE+G+H+++G GELHLE+ L  L+ ++    ++  S P+V +RE V ++S   V  KSPN
Sbjct:  385 YTSEPVVTVAIEAKNVKDLPKLIEALRQLAKEDPTLHVKIDEETGQHLLSGMGELHLEVKLYRLKTEWKLDVDV--SPPIVVYRESVTKQS-PIVEGKSPN 482          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_PICTO (Elongation factor 2 OS=Picrophilus torridus GN=fusA PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 2.755e-33
Identity = 48/128 (37.50%), Postives = 71/128 (55.47%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILS--EEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIP 681
            +HNR Y +  PL E +  AI DG+I      K +  +++  EE G   D A+ + C      G N+++DM KG+QYL+E  + ++  F  A  +G LA E + G+   + D  LH D+IHRG  QVIP
Sbjct:  483 KHNRFYFQVEPLPENVVSAILDGKIPEGSKFKDKKAVMANLEEAGLTHDEARGLVCI----YGTNVMLDMTKGIQYLDETMELLIESFNEAMDKGPLANEKVYGLKVSLMDAKLHEDSIHRGPAQVIP 606          

HSP 2 Score: 76.6406 bits (187), Expect = 2.755e-33
Identity = 38/97 (39.18%), Postives = 67/97 (69.07%), Query Frame = 2
Query:   17 PVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            PVV +A++ K  +DLPKL++ L+ ++K+DP +   + +E+GEH+I+G GELHLE+ +  +++D+    +++ S+P+V +RE V +K       KSPN
Sbjct:  389 PVVTLAIEAKHTADLPKLIDVLRTISKADPSIQVDINQETGEHLISGMGELHLEVTIYRIKNDYK--VDVVTSEPLVVYRETV-DKKGGPFEGKSPN 482          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_SULTO (Elongation factor 2 OS=Sulfolobus tokodaii GN=fusA PE=3 SV=3)

HSP 1 Score: 93.5893 bits (231), Expect = 3.588e-33
Identity = 50/126 (39.68%), Postives = 71/126 (56.35%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIP 681
            +HN+LY+   PL E   E I +G I    D K  ++IL E+  WD D AKKI          N+ V+   GVQ+L E+ D+++ GF+ A KEG LA E +RG+   + D ++H D  HRG  Q+ P
Sbjct:  490 KHNKLYISVEPLNEQTIELIANGTIKEDMDSKEMARILKEQADWDYDEAKKIVAIDENI---NVFVNATSGVQHLREVMDTILQGFRLAMKEGPLAHEPIRGLKVVLHDAIIHEDPAHRGPAQLYP 612          

HSP 2 Score: 69.3218 bits (168), Expect = 3.588e-33
Identity = 36/97 (37.11%), Postives = 66/97 (68.04%), Query Frame = 2
Query:   17 PVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            PVV ++V+ K   DL K+++ L++L+  DP ++  + EE+GE++++G G LHLE+ L+ L++++  G +++ S P+V +RE +  KS +    KSPN
Sbjct:  396 PVVTISVEPKNPKDLNKMIDALRKLSIEDPNLLVKINEETGEYLLSGMGFLHLEVSLQLLKENY--GVDVVTSPPIVVYRESIRTKS-QVFEGKSPN 489          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_METS5 (Elongation factor 2 OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348) GN=fusA PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 3.588e-33
Identity = 47/126 (37.30%), Postives = 70/126 (55.56%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIP 681
            +HN+ Y+   PL +   E I +G I    D K  +KIL ++  WD D AKKI          N+++D   GVQ+L EI D+++ G++ A +EG LA E +RG+   + D  +H D  HRG  Q+ P
Sbjct:  489 KHNKFYISVEPLNDKTIELISNGTIKEDMDSKEMAKILRDQADWDYDEAKKIIAIDENV---NVLIDATSGVQHLREIMDTMLQGYRLAMREGPLAHEPIRGVKVILHDATIHEDPAHRGPAQIYP 611          

HSP 2 Score: 72.0182 bits (175), Expect = 3.588e-33
Identity = 39/97 (40.21%), Postives = 66/97 (68.04%), Query Frame = 2
Query:   17 PVVRVAVQCKVASDLPKLVEGLKRLAKSDP-MVVCSMEESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            PVV +AV+ K   DL K+++ L++L+  DP +VV   EE+GE++++G G LH+E+ L+ L+D++  G +++ + P+V +RE V  KS +    KSPN
Sbjct:  395 PVVTIAVEPKNPKDLTKMIDALRKLSIEDPNLVVKINEETGEYLLSGMGFLHVEVSLQLLKDNY--GVDVVTTPPIVVYRESVRNKS-QVFEGKSPN 488          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_THEGJ (Elongation factor 2 OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=fusA PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 3.588e-33
Identity = 50/130 (38.46%), Postives = 72/130 (55.38%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRI--GPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPLL 687
            +HNR Y+   P+ + + EAI +G I  G   +PK  +K L+E  G D +LAK I     +    NM  D  KG+QYLNE+ D +V GF  A  EG LA+E +  +   + D  +H D +HRG  Q+ P +
Sbjct:  483 KHNRFYITVEPMPDEIYEAIREGIIPEGRPKNPKEVAKKLAE-LGMDYELAKGI----VDVYNGNMFFDNTKGIQYLNEVMDLLVDGFHMAMDEGPLAKEPVMKVIVRLHDAKIHEDNVHRGPAQIYPAI 607          

HSP 2 Score: 78.9518 bits (193), Expect = 3.588e-33
Identity = 44/101 (43.56%), Postives = 67/101 (66.34%), Query Frame = 2
Query:    5 FSVSPVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            ++  PVV VA++ K   DLPKL+E L++LAK DP +   + EE+G+H+++G GELHLE+ L  L+ ++    ++  S P+V +RE V +KS   V  KSPN
Sbjct:  385 YTSEPVVTVAIEAKNVKDLPKLIEALRQLAKEDPTLHVKIDEETGQHLLSGMGELHLEVKLYRLKTEWKLDVDV--SPPIVVYRESVTKKS-PIVEGKSPN 482          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_THESM (Elongation factor 2 OS=Thermococcus sibiricus (strain MM 739 / DSM 12597) GN=fusA PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 3.588e-33
Identity = 48/130 (36.92%), Postives = 72/130 (55.38%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRI--GPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPLL 687
            +HNR Y+   P+ + + +AI +G I  G   D KV +K L+E  G D ++A+ I     +    NM +D  KG+QYLNE+ D +V GF  A  EG LA E +  +   + D  +H D +HRG  Q+ P +
Sbjct:  483 KHNRFYIVVEPMPDEIYQAIREGEIPEGRPKDTKVVAKKLAE-LGMDYEIARGI----VDIYNGNMFLDNTKGLQYLNEVMDLLVDGFHQAMDEGPLAREPVMKVIVRLVDAKIHEDNVHRGPAQIYPAI 607          

HSP 2 Score: 80.4925 bits (197), Expect = 3.588e-33
Identity = 42/101 (41.58%), Postives = 69/101 (68.32%), Query Frame = 2
Query:    5 FSVSPVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            ++  PVV VA++ K   DLP+L+E L++LAK DP +   + EE+G+H+++G GELHLE+ L  L++ +  G ++  S+P+V +RE + ++S   V  KSPN
Sbjct:  385 YTSEPVVTVAIEAKNVKDLPRLIEALRQLAKEDPTLHVKIDEETGQHLLSGMGELHLEVKLVHLKEQW--GVDVDVSEPIVVYRESITKQS-PIVEGKSPN 482          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_PYRHO (Elongation factor 2 OS=Pyrococcus horikoshii GN=fusA PE=3 SV=2)

HSP 1 Score: 81.6481 bits (200), Expect = 6.086e-33
Identity = 45/97 (46.39%), Postives = 66/97 (68.04%), Query Frame = 2
Query:   17 PVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            PVV VA++ K   DLP+L+E L++LAK DP +   + EE+G+H+++G GELHLE+ L  L+ D+  G +I  S+P+V +RE +  KS   V  KSPN
Sbjct:  389 PVVTVAIEAKNVKDLPRLIEALRQLAKEDPTLHVKIDEETGQHLLSGMGELHLEVKLYKLKKDW--GIDIEVSEPIVVYRESI-TKSSPMVEGKSPN 482          

HSP 2 Score: 80.4925 bits (197), Expect = 6.086e-33
Identity = 47/130 (36.15%), Postives = 71/130 (54.62%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRI--GPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPLL 687
            +HNR Y+   P+ + +  AI +G I  G   +PK  +K L+E  G D ++A+ I     +    NM +D  KGVQYLNE+ D ++ GF  A  EG LA E +  +   + D  +H D +HRG  Q+ P +
Sbjct:  483 RHNRFYIVVEPMPDEIYNAIKEGIIPEGRVKNPKEVAKKLAE-LGMDYEIARGI----VDIYNGNMFIDNTKGVQYLNEVMDLLIDGFHQAMDEGPLAREPVMKVIVRLLDAQVHEDNVHRGPAQIYPAI 607          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_METMT (Elongation factor 2 OS=Methanococcoides methylutens GN=fusA PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.344e-32
Identity = 50/127 (39.37%), Postives = 71/127 (55.91%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRIGPR-DDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIP 681
            +HNR Y+   PLE  + E I +G I  R  + + R K+++   G DKD AK+I     +    N   DM KG+Q+LNE  + V+ GF    K G L++E   G+  ++ D  LH DA+HRG  QVIP
Sbjct:  484 RHNRFYVVVEPLEPEVRELIREGEISMRMPELERREKLIAA--GLDKDEAKRI----ADIFESNAYFDMTKGIQHLNETMELVLEGFVEVMKAGPLSKEPCMGVKVKLMDAKLHEDAVHRGPAQVIP 604          

HSP 2 Score: 77.7962 bits (190), Expect = 1.344e-32
Identity = 45/97 (46.39%), Postives = 63/97 (64.95%), Query Frame = 2
Query:   17 PVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            PVV VAV+ K   DLPKLVE L+++AK DP +  ++ EE+GEH++AG GELHLE+    ++ D   G EI  + P+V +RE  +  +   V  KSPN
Sbjct:  390 PVVTVAVEAKHMKDLPKLVEVLRQVAKEDPTLKITLDEETGEHLMAGMGELHLEVIAHRIERD--KGVEISTTPPIVVYRE-TITGTAGPVEGKSPN 483          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_PYRAB (Elongation factor 2 OS=Pyrococcus abyssi GN=fusA PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.751e-32
Identity = 47/130 (36.15%), Postives = 72/130 (55.38%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRI--GPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPLL 687
            +HNR Y+   P+ + +  AI +G I  G   +PK  +K L+E  G D ++A+ I     +    NM +D  KGVQYLNE+ D ++ GF  A  EG LA+E +  +   + D  +H D +HRG  Q+ P +
Sbjct:  483 RHNRFYIVVEPMPDEIYNAIKEGIIPEGRIKNPKEVAKKLAE-LGMDYEIARGI----VDVYNGNMFLDNTKGVQYLNEVMDLLIDGFHQAMDEGPLAKEPVMKVIVRLIDAQVHEDNVHRGPAQIYPAI 607          

HSP 2 Score: 80.1073 bits (196), Expect = 1.751e-32
Identity = 44/97 (45.36%), Postives = 66/97 (68.04%), Query Frame = 2
Query:   17 PVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            PVV VA++ K   DLP+L+E L++LAK DP +   + EE+G+H+++G GELHLE+ L  L+ D+  G +I  S+P+V +RE + + S   V  KSPN
Sbjct:  389 PVVTVAIEAKNVKDLPRLIEALRQLAKEDPTLHVKIDEETGQHLLSGMGELHLEVKLYKLKKDW--GIDIDVSEPIVVYRESITKPS-PMVEGKSPN 482          
BLAST of FC931933 vs. ExPASy Swiss-Prot
Match: EF2_ARCFU (Elongation factor 2 OS=Archaeoglobus fulgidus GN=fusA PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.280e-32
Identity = 45/97 (46.39%), Postives = 66/97 (68.04%), Query Frame = 2
Query:   17 PVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCSM-EESGEHIIAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFREIVLEKSCRTVMSKSPN 304
            PVV +A++ K   DLPKL+E L++LAK DP +  ++ EE+GEH+I+G GELHLE+ ++ ++ D+    ++I S P+V FRE V   S   V  KSPN
Sbjct:  388 PVVTMAIEAKNPRDLPKLIEVLRKLAKEDPTLHITLNEETGEHLISGMGELHLEVKVEKIRRDYK--LDVITSPPIVVFRETVTGTS-PVVEGKSPN 481          

HSP 2 Score: 79.337 bits (194), Expect = 2.280e-32
Identity = 44/126 (34.92%), Postives = 67/126 (53.17%), Query Frame = 1
Query:  304 QHNRLYMEARPLEEGLAEAIDDGRIGPRDDPKVRSKILSEEFGWDKDLAKKIWCFGPETIGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIP 681
            +HNR Y+   PL E + +   +G +  + D K R ++L E     ++ A        E    N+  D+ KG+QYLNE  + ++ GF+ A + G +A E   GI  ++ D  LH DA+HRG  QVIP
Sbjct:  482 KHNRFYIVVEPLPEKVIQMFKEGVVDMKMDKKERRRLLQEAGLTSEEAAG-----AEEYYEGNLFCDVTKGIQYLNETMELILEGFREAMRAGPIAREPCMGIKVKLVDCKLHEDAVHRGPAQVIP 602          
The following BLAST results are available for this feature:
BLAST of FC931933 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
EF2_THEON1.247e-3338.46Elongation factor 2 OS=Thermococcus onnurineus (st... [more]
EF2_PICTO2.755e-3337.50Elongation factor 2 OS=Picrophilus torridus GN=fus... [more]
EF2_SULTO3.588e-3339.68Elongation factor 2 OS=Sulfolobus tokodaii GN=fusA... [more]
EF2_METS53.588e-3337.30Elongation factor 2 OS=Metallosphaera sedula (stra... [more]
EF2_THEGJ3.588e-3338.46Elongation factor 2 OS=Thermococcus gammatolerans ... [more]
EF2_THESM3.588e-3336.92Elongation factor 2 OS=Thermococcus sibiricus (str... [more]
EF2_PYRHO6.086e-3346.39Elongation factor 2 OS=Pyrococcus horikoshii GN=fu... [more]
EF2_METMT1.344e-3239.37Elongation factor 2 OS=Methanococcoides methyluten... [more]
EF2_PYRAB1.751e-3236.15Elongation factor 2 OS=Pyrococcus abyssi GN=fusA P... [more]
EF2_ARCFU2.280e-3246.39Elongation factor 2 OS=Archaeoglobus fulgidus GN=f... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC34202G04EF PostHarveN Citrus clementina cDNA clone C34202G04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931933 ID=FC931933; Name=FC931933; organism=Citrus clementina; type=EST; length=712bp
GAAGTTTTCCGTCTCACCTGTGGTTCGTGTGGCTGTACAGTGCAAAGTTG
CATCTGATCTCCCTAAGCTTGTTGAAGGTCTTAAACGGTTGGCCAAGTCA
GATCCCATGGTTGTCTGTTCCATGGAGGAATCTGGAGAGCATATCATTGC
CGGTGCAGGAGAACTCCATCTTGAGATCTGTTTGAAGGATTTGCAGGATG
ATTTCATGGGTGGTGCTGAGATTATCAAATCTGATCCTGTTGTGTCCTTC
CGTGAGATTGTCCTTGAGAAGTCCTGCCGTACTGTTATGAGCAAGTCTCC
AAACAACACAATCGTCTCTATATGGAAGCCCGGCCATTGGAGGAAGGTCT
TGCTGAGGCAATTGATGATGGTCGTATTGGTCCAAGGGATGATCCTAAGG
TTCGTTCTAAGATTCTGTCTGAGGAATTTGGTTGGGACAAGGATCTTGCC
AAGAAAATCTGGTGTTTTGGTCCTGAGACCATTGGTCCTAACATGGTTGT
GGATATGTGCAAGGGAGTTCAGTACTTGAATGAAATTAAGGATTCAGTTG
TTGCTGGGTTCCAGTGGGCATCAAAGGAAGGTGCATTGGCTGAAGAAAAC
ATGAGGGGCATTTGCTTTGAAGTTTGTGATGTGGTTCTTCATGCCGATGC
TATCCACAGAGGTGGTGGCCAGGTTATTCCACTGCTAGAANGGTCATTTA
TACATCCCAGCT
back to top