FC932456

Overview
NameFC932456
Unique NameFC932456
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length750
Libraries
Library NameType
PostHarveNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_THET8 (Phosphoenolpyruvate carboxylase OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=ppc PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 7.846e-27
Identity = 65/165 (39.39%), Postives = 89/165 (53.94%), Query Frame = 1 
Query:    1 KFGLPQTAVRQLEIYTTAVLLATMRPPQPPREEKWRNLMEEISKISCQNYRSTVYENPEFLAYFNEATPQAELGYLNIGSRPTRRKSST-GIGHLRAIPWVFAWTQTRFVLPAWLGIGAGLKGVCDMGNTEDWKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRY 492
            ++  P  AVR LE       LA + P Q P E +WR  + + ++ S + YR+ + E   F  +F   TP  E+G L I SRP  R+     I  LRAIPWV AWTQ R +LP W G+ A  +   D+      +EMY+ WPFF ST++   M L KAD  +A+ Y
Sbjct:  605 RYSHPDLAVRHLEQMLYHFALAALGPGQEP-EARWREALAQAAEESTRRYRALLQEEG-FFDFFEAFTPIREIGELPIASRPVYRRGRVRDIRDLRAIPWVMAWTQVRVLLPGWYGLSALEELPLDL-----LREMYRGWPFFASTLEAAAMALAKADMGVARLY 762

HSP 2 Score: 31.187 bits (69), Expect = 7.846e-27
Identity = 13/28 (46.43%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  615 SLRRLIESRLPYLNPMNMLXVEILKRLR 698
            +L R I  R PY++P+N++ VE+L+R R
Sbjct:  802 TLERQIRLRNPYVDPINIVQVELLRRYR 829
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_THET2 (Phosphoenolpyruvate carboxylase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=ppc PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 7.846e-27
Identity = 65/165 (39.39%), Postives = 89/165 (53.94%), Query Frame = 1 
Query:    1 KFGLPQTAVRQLEIYTTAVLLATMRPPQPPREEKWRNLMEEISKISCQNYRSTVYENPEFLAYFNEATPQAELGYLNIGSRPTRRKSST-GIGHLRAIPWVFAWTQTRFVLPAWLGIGAGLKGVCDMGNTEDWKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRY 492
            ++  P  AVR LE       LA + P Q P E +WR  + + ++ S + YR+ + E   F  +F   TP  E+G L I SRP  R+     I  LRAIPWV AWTQ R +LP W G+ A  +   D+      +EMY+ WPFF ST++   M L KAD  +A+ Y
Sbjct:  605 RYSHPDLAVRHLEQMLYHFALAALGPGQEP-EARWREALAQAAEESTRRYRALLQEEG-FFDFFEAFTPIREIGELPIASRPVYRRGRVRDIRDLRAIPWVMAWTQVRVLLPGWYGLSALEELPLDL-----LREMYRGWPFFASTLEAAAMALAKADMGVARLY 762

HSP 2 Score: 31.187 bits (69), Expect = 7.846e-27
Identity = 13/28 (46.43%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  615 SLRRLIESRLPYLNPMNMLXVEILKRLR 698
            +L R I  R PY++P+N++ VE+L+R R
Sbjct:  802 TLERQIRLRNPYVDPINIVQVELLRRYR 829
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_CAUCR (Phosphoenolpyruvate carboxylase OS=Caulobacter crescentus GN=ppc PE=3 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 8.032e-26
Identity = 73/206 (35.44%), Postives = 113/206 (54.85%), Query Frame = 1 
Query:    1 KFGLPQTAVRQLEIYTTAVLLATMRPPQPPREEKWRNLMEEISKISCQNYRSTVYENPEFLAYFNEATPQAELGYLNIGSRPTRRKSSTGIGHLRAIPWVFAWTQTRFVLPAWLGIGAGLK--GVCDMGNTEDWKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRYDEVLVSESRQE-LGAELRRELLTTEKFVLVVSGHEKLSEN 609
            +FG   TA R L+    AVL+A+ RP  P R+ K    M  +++ S + YR+ VY++  F  +F   TP +E+  LNIGSRP  R +S  I  LRAIPWVF+W+Q RF+LP W G  +G++  G+    + E  +++   + FF S +  +E+ L ++   IA RY  +   ++  E + A +RRE        L + G   L +N
Sbjct:  652 RFGDQPTARRNLDGLAAAVLMASERPI-PKRDPKVEGAMTALAQSSFEGYRALVYDDAAFEDFFWSVTPISEIVGLNIGSRPASRTASRKIEDLRAIPWVFSWSQARFMLPGWYGFASGVERAGL----SVEQLRDLAGNFDFFASLLSNMELALAQSQMGIAARYVALSPDKANAERIFATIRREHEAATGLALAIRGGAALLDN 852
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_BORPA (Phosphoenolpyruvate carboxylase OS=Bordetella parapertussis GN=ppc PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 5.682e-24
Identity = 64/209 (30.62%), Postives = 105/209 (50.24%), Query Frame = 1 
Query:   34 LEIYTTAVLLATMRPP------QPPREEKWRNLMEEISKISCQNYRSTVYENPEFLAYFNEATPQAELGYLNIGSRPTRRKSSTGIGHLRAIPWVFAWTQTRFVLPAWLGIGAGLKGVCDMG----------NTEDWKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRYDEVLVSES-RQELGAELRRELLTTEKFVLVVSGHEKLSEN 609
            LE+   A L +++ P       +    ++    M  +S+++ + YR  VY+ P F  YF  ATP +E+  LNIGSRP  RK    I  LRAIPW F+W Q R +L  W G+G+ ++   + G               +EM  +WP F++ +  +EMVL K+D  IA  Y +++     R+ +   +  E   T   + +++  E L++N
Sbjct:  712 LELLVAATLESSLAPQAAATSAEDAHMQQHAPAMSFMSELAQRTYRGLVYDTPGFADYFFAATPISEIAGLNIGSRPASRKKGQHIEDLRAIPWGFSWAQCRLMLTGWYGMGSAIEAYLETGAQGAPRSRRARLAQLREMASDWPAFRTLLSNMEMVLAKSDLAIAAGYAQLVPRRGLRERVFGAITAEHGRTLAMLRLLTRRELLADN 920

HSP 2 Score: 26.5646 bits (57), Expect = 5.682e-24
Identity = 9/23 (39.13%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  630 IESRLPYLNPMNMLXVEILKRLR 698
            +  R  Y++P+N L +E++KR R
Sbjct:  927 LRERFAYIDPLNYLQIELIKRHR 949
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_BORBR (Phosphoenolpyruvate carboxylase OS=Bordetella bronchiseptica GN=ppc PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 5.682e-24
Identity = 64/209 (30.62%), Postives = 105/209 (50.24%), Query Frame = 1 
Query:   34 LEIYTTAVLLATMRPP------QPPREEKWRNLMEEISKISCQNYRSTVYENPEFLAYFNEATPQAELGYLNIGSRPTRRKSSTGIGHLRAIPWVFAWTQTRFVLPAWLGIGAGLKGVCDMG----------NTEDWKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRYDEVLVSES-RQELGAELRRELLTTEKFVLVVSGHEKLSEN 609
            LE+   A L +++ P       +    ++    M  +S+++ + YR  VY+ P F  YF  ATP +E+  LNIGSRP  RK    I  LRAIPW F+W Q R +L  W G+G+ ++   + G               +EM  +WP F++ +  +EMVL K+D  IA  Y +++     R+ +   +  E   T   + +++  E L++N
Sbjct:  712 LELLVAATLESSLAPQAAATSAEDAHMQQHAPAMSFMSELAQRTYRGLVYDTPGFADYFFAATPISEIAGLNIGSRPASRKKGQHIEDLRAIPWGFSWAQCRLMLTGWYGMGSAIEAYLETGAQGAPRSRRARLAQLREMASDWPAFRTLLSNMEMVLAKSDLAIAAGYAQLVPRRGLRERVFGAITAEHGRTLAMLRLLTRRELLADN 920

HSP 2 Score: 26.5646 bits (57), Expect = 5.682e-24
Identity = 9/23 (39.13%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  630 IESRLPYLNPMNMLXVEILKRLR 698
            +  R  Y++P+N L +E++KR R
Sbjct:  927 LRERFAYIDPLNYLQIELIKRHR 949
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_THES7 (Phosphoenolpyruvate carboxylase OS=Thermus sp. (strain 71) GN=ppc PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 5.694e-24
Identity = 73/193 (37.82%), Postives = 96/193 (49.74%), Query Frame = 1 
Query:    1 KFGLPQTAVRQLEIYTTAVLLATMRPPQPPREEKWRNLMEEISKISCQNYRSTVYENPEFLAYFNEATPQAELGYLNIGSRPTRRKSST-GIGHLRAIPWVFAWTQTRFVLPAWLGIGAGLKGVCDMGNTEDWKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRYDEVLVSESRQ----ELGAELRRELLTTE 564
            ++  P  AVR LE        A +     P+   WR  + E  + S   YR+ + +   F  +F   TP  E+G L I SRP  R      I  LRAIPWV AWTQ R +LP W G+ A L+G+         +EMY+EWPFF +T++   M L KAD  IA+RY + LV E  Q     L  E RR +   E
Sbjct:  604 RYAHPDLAVRHLEQLLYHFAQAALGDGVEPKAH-WREALGEAGERSMARYRALLSQEG-FFPFFEAFTPIREIGELPIASRPVYRHGRVRDIRDLRAIPWVMAWTQVRLLLPGWYGLSA-LEGL----PMPLLREMYREWPFFATTLESAAMALAKADLGIAERYLK-LVPEGLQGFYHHLAEEYRRTVALLE 788

HSP 2 Score: 29.261 bits (64), Expect = 5.694e-24
Identity = 13/28 (46.43%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  615 SLRRLIESRLPYLNPMNMLXVEILKRLR 698
            +L R I  R PY++P+N + VE+L R R
Sbjct:  801 TLERQIALRNPYVDPINFVQVELLARYR 828
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_BORPE (Phosphoenolpyruvate carboxylase OS=Bordetella pertussis GN=ppc PE=3 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 9.621e-24
Identity = 59/180 (32.78%), Postives = 92/180 (51.11%), Query Frame = 1 
Query:   34 LEIYTTAVLLATMRPP------QPPREEKWRNLMEEISKISCQNYRSTVYENPEFLAYFNEATPQAELGYLNIGSRPTRRKSSTGIGHLRAIPWVFAWTQTRFVLPAWLGIGAGLKGVCDMG----------NTEDWKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRYDEVLVSESRQE 525
            LE+   A L +++ P       +    ++    M  +S+++ + YR  VY+ P F  YF  ATP +E+  LNIGSRP  RK    I  LRAIPW F+W Q R +L  W G+G+ ++   + G               +EM  +WP F++ +  +EMVL K+D  IA  Y +++     +E
Sbjct:  710 LELLVAATLESSLAPQAAATSAEDAHMQQHAPAMSFMSELAQRTYRGLVYDTPGFADYFFAATPISEIAGLNIGSRPASRKKGQHIEDLRAIPWGFSWAQCRLMLTGWYGMGSAIEAYLETGAQGAPRSRRARLAQLREMASDWPAFRTLLSNMEMVLAKSDLAIAAGYAQLVPRRGLRE 889

HSP 2 Score: 26.5646 bits (57), Expect = 9.621e-24
Identity = 9/23 (39.13%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  630 IESRLPYLNPMNMLXVEILKRLR 698
            +  R  Y++P+N L +E++KR R
Sbjct:  925 LRERFAYIDPLNYLQIELIKRHR 947
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_RHOMR (Phosphoenolpyruvate carboxylase OS=Rhodothermus marinus GN=ppc PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.098e-21
Identity = 59/176 (33.52%), Postives = 92/176 (52.27%), Query Frame = 1 
Query:    1 KFGLPQTAVRQLEIYTTAVLLATMRPPQPPRE---EKWRN-LMEEISKISCQNYRSTVYENPEFLAYFNEATPQAELGYLNIGSRPTRRKSSTGIGH--LRAIPWVFAWTQTRFVLPAWLGIGAGLKGVCDMG--NTEDWKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRYDEVL 504
            ++ LP+ A R LE    A+L     P     +      RN LM+E++  S + YR  + + P+F +++   TP  ++  L I SRP  R S+  +    LRAIPWVFAWTQ R+++P W GIG  L  +      + E  +  Y+ WPFF++ +   +  + +A   IA  YD +L
Sbjct:  669 RYALPEIAHRHLEQIVNAMLRVVGLPAASGTDGTDPATRNRLMDELAARSMRAYRRLI-DAPDFWSWYTRITPIDQISRLPIASRPVSRSSAREVDFESLRAIPWVFAWTQVRYLIPGWFGIGQALDELLQTSPEHLETLRTWYRSWPFFRTVLQNAQREMVRARLEIAAYYDRLL 843

HSP 2 Score: 26.9498 bits (58), Expect = 1.098e-21
Identity = 8/27 (29.63%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  618 LRRLIESRLPYLNPMNMLXVEILKRLR 698
            +R+ ++ R PY + +N++ +E+++R+R
Sbjct:  881 IRKSVQLRNPYTDVLNLVQLELMRRIR 907
[Close]
BLAST of FC932456 vs. ExPASy Swiss-Prot
Match: CAPP_METEA (Phosphoenolpyruvate carboxylase OS=Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) GN=ppc PE=3 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 1.242e-18
Identity = 51/144 (35.42%), Postives = 77/144 (53.47%), Query Frame = 1 
Query:  187 YFNEATPQAELGYLNIGSRPTRRKSSTGIGHLRAIPWVFAWTQTRFVLPAWLGIGAGLKGVCDMGNTED---WKEMYKEWPFFQSTIDLIEMVLGKADTHIAKRYDEVLVSES-RQELGAELRRELLTTEKFVLVVSGHEKLSE 606
            YF  A+P  E+  LNIGSRP RR  +  +  LRAIPWVFAW+Q R V+  W G+G+GLK   D+         + ++++   F+  +D +E  L   D  IA+ Y  ++     R  +   +  E   T + VL VSG  +L++
Sbjct:  718 YFQAASPLDEISLLNIGSRPARRFGAKSLSDLRAIPWVFAWSQNRHVITGWYGVGSGLKSFIDVRGEAGEALLRRLFRDCRVFRLVLDEVEKTLLMVDLEIARDYAGLVEDAGIRARIFGMIEAEYALTREMVLRVSGDSELAQ 861
The following BLAST results are available for this feature:
BLAST of FC932456 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 229
Match NameE-valueIdentityDescription
CAPP_THET87.846e-2739.39Phosphoenolpyruvate carboxylase OS=Thermus thermop... [more]
CAPP_THET27.846e-2739.39Phosphoenolpyruvate carboxylase OS=Thermus thermop... [more]
CAPP_CAUCR8.032e-2635.44Phosphoenolpyruvate carboxylase OS=Caulobacter cre... [more]
CAPP_BORPA5.682e-2430.62Phosphoenolpyruvate carboxylase OS=Bordetella para... [more]
CAPP_BORBR5.682e-2430.62Phosphoenolpyruvate carboxylase OS=Bordetella bron... [more]
CAPP_THES75.694e-2437.82Phosphoenolpyruvate carboxylase OS=Thermus sp. (st... [more]
CAPP_BORPE9.621e-2432.78Phosphoenolpyruvate carboxylase OS=Bordetella pert... [more]
CAPP_RHOMR1.098e-2133.52Phosphoenolpyruvate carboxylase OS=Rhodothermus ma... [more]
CAPP_METEA1.242e-1835.42Phosphoenolpyruvate carboxylase OS=Methylobacteriu... [more]

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Properties
Property NameValue
Genbank descriptionC34208G08EF PostHarveN Citrus clementina cDNA clone C34208G08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC932456 ID=FC932456; Name=FC932456; organism=Citrus clementina; type=EST; length=750bp
AAGTTTGGGTTGCCACAGACAGCTGTTAGACAGCTAGAGATATACACAAC
TGCTGTGCTGCTTGCAACCATGCGACCTCCTCAACCTCCTCGAGAAGAAA
AATGGCGTAATCTCATGGAGGAGATCTCAAAAATTAGTTGCCAGAATTAC
CGGAGCACAGTCTATGAAAACCCAGAATTCCTTGCCTACTTCAACGAGGC
TACACCCCAGGCTGAACTAGGGTACCTTAATATAGGAAGCCGCCCCACAA
GAAGAAAGAGCTCAACAGGAATTGGCCATCTTCGAGCCATTCCATGGGTA
TTTGCATGGACCCAGACCAGGTTTGTCCTTCCAGCTTGGCTTGGAATCGG
AGCAGGGCTGAAGGGTGTTTGTGACATGGGAAATACTGAAGACTGGAAAG
AAATGTATAAAGAATGGCCCTTTTTCCAGTCTACCATAGATCTTATAGAG
ATGGTTTTAGGGAAGGCAGACACTCATATAGCCAAGCGCTATGATGAAGT
CCTTGTTTCAGAGAGTAGGCAAGAGCTTGGTGCTGAGCTAAGGAGGGAGC
TCTTAACAACTGAGAAGTTCGTACTTGTGGTTAGTGGGCATGAGAAGCTA
TCTGAGAATACCACAGTTTGAGGAGGCTGATTGAGAGCAGACTCCCATAT
CTCAATCCTATGAATATGTTGCANGTGGAGATACTGAAGAGATTGAGACA
GGATGATGATAACCATAACTCAGAGATGCATTGCTCATCACTATAAATGG
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