BQ623021

Overview
NameBQ623021
Unique NameBQ623021
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length526
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC21_CAEEL (Probable ubiquitin-conjugating enzyme E2 21 OS=Caenorhabditis elegans GN=ubc-21 PE=2 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 1.186e-14
Identity = 29/71 (40.85%), Postives = 46/71 (64.79%), Query Frame = 2 
Query:  110 NINSN-GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            NI+S  G+ICLDILK++W+ +LT+  VLLS+ ++L  P P DP    +A  + ++   + +TA  WT  +A
Sbjct:  112 NISSQTGTICLDILKDKWTASLTLRTVLLSLQAMLCSPEPSDPQDAVVAKQFINNYPMFTATAVYWTSYFA 182

HSP 2 Score: 40.0466 bits (92), Expect = 1.186e-14
Identity = 19/54 (35.19%), Postives = 26/54 (48.15%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVA---------------FRTKVFHPEHQQQ 123
            +PYAGG F + +  P  YPF+PPKV                F T+++HP    Q
Sbjct:   63 TPYAGGTFEIKVDIPEHYPFEPPKVTEIIFHIRAFEYIQAKFVTRIWHPNISSQ 116
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_RANCA (Ubiquitin-conjugating enzyme E2 S OS=Rana catesbeiana GN=ube2s PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.189e-14
Identity = 30/77 (38.96%), Postives = 43/77 (55.84%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAMG*RLLG 340
            N++SNG IC+++LK+ W   L I  VLL+I  LL  PNP+  L  E   +   +  +Y S AR  T+ +A    L G
Sbjct:   87 NVSSNGEICVNVLKKDWKAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRARLMTEIHAHSSSLRG 163

HSP 2 Score: 42.743 bits (99), Expect = 1.189e-14
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYAGG+F + +    D+P  PPK  F TK+FHP
Sbjct:   53 TPYAGGIFRMKLILGKDFPAAPPKGYFLTKIFHP 86
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC11_YEAST (Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharomyces cerevisiae GN=UBC11 PE=1 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.206e-14
Identity = 25/58 (43.10%), Postives = 38/58 (65.52%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY 283
            N++ +G+ICLDILKE+WS    +  +LLS+ SLL +PN   PL    A ++ +D  +Y
Sbjct:   85 NVDKSGNICLDILKEKWSAVYNVETILLSLQSLLGEPNNRSPLNAVAAELWDADMEEY 142

HSP 2 Score: 43.1282 bits (100), Expect = 1.206e-14
Identity = 16/35 (45.71%), Postives = 25/35 (71.43%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            D+PY+G  F V++ FP +YPF PP + F + ++HP
Sbjct:   50 DTPYSGLKFKVSLKFPQNYPFHPPMIKFLSPMWHP 84
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC16_SCHPO (Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe GN=ubc16 PE=2 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 2.035e-14
Identity = 28/70 (40.00%), Postives = 41/70 (58.57%), Query Frame = 2 
Query:  119 SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKS-DKAKYESTARSWTQKYAMG 325
            +NG +C+DILK  WSPA ++    L+I SLL++ +   PL  + A + ++ DK  Y S  R  T  YA G
Sbjct:   85 TNGEVCMDILKTHWSPAWSLQSACLAIISLLSNYDASSPLNVDAAKLLRTGDKTAYNSLVRCTTYLYAKG 154

HSP 2 Score: 43.1282 bits (100), Expect = 2.035e-14
Identity = 16/36 (44.44%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +++PY GG +++ IH    YP  PP V F+TK+ HP
Sbjct:   45 TETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHP 80
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC9_XENTR (SUMO-conjugating enzyme UBC9 OS=Xenopus tropicalis GN=ube2i PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.644e-14
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  +G++CL IL+E   W PA+TI ++LL I  LL +PN  DP   E   +Y  ++ +YE   R+  +K+A
Sbjct:   85 NVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFA 156

HSP 2 Score: 38.891 bits (89), Expect = 2.644e-14
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+ GG+F + + F  DYP  PPK  F   +FHP
Sbjct:   51 TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHP 84
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC9_XENLA (SUMO-conjugating enzyme UBC9 OS=Xenopus laevis GN=ube2i PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.644e-14
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  +G++CL IL+E   W PA+TI ++LL I  LL +PN  DP   E   +Y  ++ +YE   R+  +K+A
Sbjct:   85 NVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFA 156

HSP 2 Score: 38.891 bits (89), Expect = 2.644e-14
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+ GG+F + + F  DYP  PPK  F   +FHP
Sbjct:   51 TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHP 84
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC9_SPETR (SUMO-conjugating enzyme UBC9 OS=Spermophilus tridecemlineatus GN=UBE2I PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.644e-14
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  +G++CL IL+E   W PA+TI ++LL I  LL +PN  DP   E   +Y  ++ +YE   R+  +K+A
Sbjct:   85 NVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFA 156

HSP 2 Score: 38.891 bits (89), Expect = 2.644e-14
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+ GG+F + + F  DYP  PPK  F   +FHP
Sbjct:   51 TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHP 84
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC9_RAT (SUMO-conjugating enzyme UBC9 OS=Rattus norvegicus GN=Ube2i PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.644e-14
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  +G++CL IL+E   W PA+TI ++LL I  LL +PN  DP   E   +Y  ++ +YE   R+  +K+A
Sbjct:   85 NVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFA 156

HSP 2 Score: 38.891 bits (89), Expect = 2.644e-14
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+ GG+F + + F  DYP  PPK  F   +FHP
Sbjct:   51 TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHP 84
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC9_MOUSE (SUMO-conjugating enzyme UBC9 OS=Mus musculus GN=Ube2i PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.644e-14
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  +G++CL IL+E   W PA+TI ++LL I  LL +PN  DP   E   +Y  ++ +YE   R+  +K+A
Sbjct:   85 NVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFA 156

HSP 2 Score: 38.891 bits (89), Expect = 2.644e-14
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+ GG+F + + F  DYP  PPK  F   +FHP
Sbjct:   51 TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHP 84
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC9_HUMAN (SUMO-conjugating enzyme UBC9 OS=Homo sapiens GN=UBE2I PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.644e-14
Identity = 28/72 (38.89%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  +G++CL IL+E   W PA+TI ++LL I  LL +PN  DP   E   +Y  ++ +YE   R+  +K+A
Sbjct:   85 NVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFA 156

HSP 2 Score: 38.891 bits (89), Expect = 2.644e-14
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+ GG+F + + F  DYP  PPK  F   +FHP
Sbjct:   51 TPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHP 84
The following BLAST results are available for this feature:
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201
Match NameE-valueIdentityDescription
UBC21_CAEEL1.186e-1440.85Probable ubiquitin-conjugating enzyme E2 21 OS=Cae... [more]
UBE2S_RANCA1.189e-1438.96Ubiquitin-conjugating enzyme E2 S OS=Rana catesbei... [more]
UBC11_YEAST1.206e-1443.10Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharo... [more]
UBC16_SCHPO2.035e-1440.00Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccha... [more]
UBC9_XENTR2.644e-1438.89SUMO-conjugating enzyme UBC9 OS=Xenopus tropicalis... [more]
UBC9_XENLA2.644e-1438.89SUMO-conjugating enzyme UBC9 OS=Xenopus laevis GN=... [more]
UBC9_SPETR2.644e-1438.89SUMO-conjugating enzyme UBC9 OS=Spermophilus tride... [more]
UBC9_RAT2.644e-1438.89SUMO-conjugating enzyme UBC9 OS=Rattus norvegicus ... [more]
UBC9_MOUSE2.644e-1438.89SUMO-conjugating enzyme UBC9 OS=Mus musculus GN=Ub... [more]
UBC9_HUMAN2.644e-1438.89SUMO-conjugating enzyme UBC9 OS=Homo sapiens GN=UB... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA
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