BQ623021

Overview
NameBQ623021
Unique NameBQ623021
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length526
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROER (Ubiquitin-conjugating enzyme E2 S OS=Drosophila erecta GN=GG18165 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.118e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 43.1282 bits (100), Expect = 2.118e-13
Identity = 18/34 (52.94%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA GVF V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAAGVFRVKLTLNKDFPQTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROGR (Ubiquitin-conjugating enzyme E2 S OS=Drosophila grimshawi GN=GH12079 PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.120e-13
Identity = 28/70 (40.00%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ SNG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVASNGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 41.9726 bits (97), Expect = 2.120e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA G+F V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC8_YEAST (Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharomyces cerevisiae GN=UBC8 PE=1 SV=2)

HSP 1 Score: 51.2174 bits (121), Expect = 2.747e-13
Identity = 26/68 (38.24%), Postives = 37/68 (54.41%), Query Frame = 2 
Query:  119 SNGSICLDILKEQWSPALTISKVL-LSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            ++GSICLD++   WSP   +  ++   I  LL +PN  DPL  E A +   DK  YE   + +  KYA
Sbjct:   80 ASGSICLDVINSTWSPLYDLINIVEWMIPGLLKEPNGSDPLNNEAATLQLRDKKLYEEKIKEYIDKYA 147

HSP 2 Score: 43.5134 bits (101), Expect = 2.747e-13
Identity = 15/35 (42.86%), Postives = 23/35 (65.71%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            D+PY  GV+ + +  P +YP+K P + F  K+FHP
Sbjct:   41 DTPYENGVWRLHVELPDNYPYKSPSIGFVNKIFHP 75
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROAN (Ubiquitin-conjugating enzyme E2 S OS=Drosophila ananassae GN=GF21161 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.752e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 42.743 bits (99), Expect = 2.752e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA G+F V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAAGIFRVKLTLNKDFPQTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_HUMAN (Ubiquitin-conjugating enzyme E2 S OS=Homo sapiens GN=UBE2S PE=1 SV=2)

HSP 1 Score: 51.9878 bits (123), Expect = 3.567e-13
Identity = 25/67 (37.31%), Postives = 38/67 (56.72%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 310
            N+ +NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   87 NVGANGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153

HSP 2 Score: 42.3578 bits (98), Expect = 3.567e-13
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYAGG+F + +    D+P  PPK  F TK+FHP
Sbjct:   53 TPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHP 86
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_BOVIN (Ubiquitin-conjugating enzyme E2 S OS=Bos taurus GN=UBE2S PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 3.567e-13
Identity = 25/67 (37.31%), Postives = 38/67 (56.72%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 310
            N+ +NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   87 NVGANGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153

HSP 2 Score: 42.3578 bits (98), Expect = 3.567e-13
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYAGG+F + +    D+P  PPK  F TK+FHP
Sbjct:   53 TPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHP 86
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2C_SPISO (Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima GN=UBE2C PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 3.603e-13
Identity = 25/59 (42.37%), Postives = 41/59 (69.49%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N++ +G+ICLDILKE W+ +  +  +LLS+ SLL +PN   PL  + A M+ S++ +Y+
Sbjct:  106 NVDQSGNICLDILKENWTASYDVRTILLSLQSLLGEPNNASPLNAQAADMW-SNQTEYK 163

HSP 2 Score: 41.2022 bits (95), Expect = 3.603e-13
Identity = 17/39 (43.59%), Postives = 23/39 (58.97%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPEHQQ 120
            D+ Y    + +T+ FP DYP+KPP V F T  +HP   Q
Sbjct:   71 DTVYESLKYKLTLEFPSDYPYKPPVVKFTTPCWHPNVDQ 109
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC9_YEAST (SUMO-conjugating enzyme UBC9 OS=Saccharomyces cerevisiae GN=UBC9 PE=1 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 3.629e-13
Identity = 23/72 (31.94%), Postives = 40/72 (55.56%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  +G+ICL IL E   W PA+T+ +++L +  LL  PNP+ P        +  +KA+Y+       ++Y+
Sbjct:   85 NVYPSGTICLSILNEDQDWRPAITLKQIVLGVQDLLDSPNPNSPAQEPAWRSFSRNKAEYDKKVLLQAKQYS 156

HSP 2 Score: 43.5134 bits (101), Expect = 3.629e-13
Identity = 16/32 (50.00%), Postives = 23/32 (71.88%), Query Frame = 1 
Query:   13 YAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +AGGV+ +T+ +P +YP KPPKV F    +HP
Sbjct:   53 WAGGVYPITVEYPNEYPSKPPKVKFPAGFYHP 84
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROWI (Ubiquitin-conjugating enzyme E2 S OS=Drosophila willistoni GN=GK16201 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 4.646e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 41.9726 bits (97), Expect = 4.646e-13
Identity = 18/34 (52.94%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA GVF V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAIGVFRVKLTLSKDFPQTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROMO (Ubiquitin-conjugating enzyme E2 S OS=Drosophila mojavensis GN=GI11085 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 4.646e-13
Identity = 27/70 (38.57%), Postives = 37/70 (52.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 41.9726 bits (97), Expect = 4.646e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA G+F V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHP 89
The following BLAST results are available for this feature:
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201
Match NameE-valueIdentityDescription
UBE2S_DROER2.118e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila er... [more]
UBE2S_DROGR2.120e-1340.00Ubiquitin-conjugating enzyme E2 S OS=Drosophila gr... [more]
UBC8_YEAST2.747e-1338.24Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharo... [more]
UBE2S_DROAN2.752e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila an... [more]
UBE2S_HUMAN3.567e-1337.31Ubiquitin-conjugating enzyme E2 S OS=Homo sapiens ... [more]
UBE2S_BOVIN3.567e-1337.31Ubiquitin-conjugating enzyme E2 S OS=Bos taurus GN... [more]
UBE2C_SPISO3.603e-1342.37Ubiquitin-conjugating enzyme E2 C OS=Spisula solid... [more]
UBC9_YEAST3.629e-1331.94SUMO-conjugating enzyme UBC9 OS=Saccharomyces cere... [more]
UBE2S_DROWI4.646e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila wi... [more]
UBE2S_DROMO4.646e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila mo... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA
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