BQ623021

Overview
NameBQ623021
Unique NameBQ623021
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length526
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC4_WHEAT (Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum GN=UBC4 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 4.672e-13
Identity = 24/67 (35.82%), Postives = 39/67 (58.21%), Query Frame = 2 
Query:  122 NGSICLDILKEQWSPALTISKVL-LSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            +GS+CLD++ + WSP   +  +  + +  LL  PNP DPL  E A +   DK  YE+  + + ++YA
Sbjct:   81 SGSVCLDVINQTWSPMFDLVNIFEVFLPQLLLYPNPSDPLNGEAASLMMRDKNAYENKVKEYCERYA 147

HSP 2 Score: 40.0466 bits (92), Expect = 4.672e-13
Identity = 15/35 (42.86%), Postives = 21/35 (60.00%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DS Y GGV+ V +     YP+K P + F  K++HP
Sbjct:   41 DSIYQGGVWKVRVELTEAYPYKSPSIGFTNKIYHP 75
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_RAT (Ubiquitin-conjugating enzyme E2 S OS=Rattus norvegicus GN=Ube2s PE=2 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 6.018e-13
Identity = 25/67 (37.31%), Postives = 37/67 (55.22%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 310
            N+  NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   87 NVGPNGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153

HSP 2 Score: 42.3578 bits (98), Expect = 6.018e-13
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYAGG+F + +    D+P  PPK  F TK+FHP
Sbjct:   53 TPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHP 86
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_MOUSE (Ubiquitin-conjugating enzyme E2 S OS=Mus musculus GN=Ube2s PE=2 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 6.018e-13
Identity = 25/67 (37.31%), Postives = 37/67 (55.22%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 310
            N+  NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   87 NVGPNGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153

HSP 2 Score: 42.3578 bits (98), Expect = 6.018e-13
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYAGG+F + +    D+P  PPK  F TK+FHP
Sbjct:   53 TPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHP 86
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2SA_XENLA (Ubiquitin-conjugating enzyme E2 S-A OS=Xenopus laevis GN=ube2s-A PE=2 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 6.018e-13
Identity = 25/67 (37.31%), Postives = 37/67 (55.22%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQ 310
            N+  NG IC+++LK  W+  L I  VLL+I  LL  PNP+  L  E   +   +  +Y + AR  T+
Sbjct:   87 NVGPNGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153

HSP 2 Score: 42.3578 bits (98), Expect = 6.018e-13
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYAGG+F + +    D+P  PPK  F TK+FHP
Sbjct:   53 TPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHP 86
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROPS (Ubiquitin-conjugating enzyme E2 S OS=Drosophila pseudoobscura pseudoobscura GN=GA22810 PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 6.032e-13
Identity = 27/70 (38.57%), Postives = 36/70 (51.43%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAGNGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 41.9726 bits (97), Expect = 6.032e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA G+F V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2S_DROPE (Ubiquitin-conjugating enzyme E2 S OS=Drosophila persimilis GN=GL16001 PE=3 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 6.032e-13
Identity = 27/70 (38.57%), Postives = 36/70 (51.43%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            N+  NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   90 NVAGNGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 41.9726 bits (97), Expect = 6.032e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PYA G+F V +    D+P  PPK  F TK+FHP
Sbjct:   56 TPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHP 89
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2H_MOUSE (Ubiquitin-conjugating enzyme E2 H OS=Mus musculus GN=Ube2h PE=2 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 6.070e-13
Identity = 22/68 (32.35%), Postives = 38/68 (55.88%), Query Frame = 2 
Query:  119 SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   82 ASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 45.8246 bits (107), Expect = 6.070e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PY GGV+ V +  P  YPFK P + F  K+FHP
Sbjct:   44 TPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHP 77
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2H_HUMAN (Ubiquitin-conjugating enzyme E2 H OS=Homo sapiens GN=UBE2H PE=1 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 6.070e-13
Identity = 22/68 (32.35%), Postives = 38/68 (55.88%), Query Frame = 2 
Query:  119 SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   82 ASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 45.8246 bits (107), Expect = 6.070e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PY GGV+ V +  P  YPFK P + F  K+FHP
Sbjct:   44 TPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHP 77
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2H_BOVIN (Ubiquitin-conjugating enzyme E2 H OS=Bos taurus GN=UBE2H PE=2 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 6.070e-13
Identity = 22/68 (32.35%), Postives = 38/68 (55.88%), Query Frame = 2 
Query:  119 SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   82 ASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 45.8246 bits (107), Expect = 6.070e-13
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PY GGV+ V +  P  YPFK P + F  K+FHP
Sbjct:   44 TPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHP 77
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC_ASFWA (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (isolate Warthog/Namibia/Wart80/1980) GN=UBC PE=2 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 7.817e-13
Identity = 26/60 (43.33%), Postives = 35/60 (58.33%), Query Frame = 2 
Query:  110 NINSNGSICLDIL-----KEQ---WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 265
            NI  +G +C+ IL     +EQ   WSPA  I  +LLS+ SLL +PNPD P   + A  Y+
Sbjct:   77 NIYPDGKLCISILHGDNAEEQGMTWSPAQKIDTILLSVISLLNEPNPDSPANVDAAKSYR 136

HSP 2 Score: 45.4394 bits (106), Expect = 7.817e-13
Identity = 15/35 (42.86%), Postives = 26/35 (74.29%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            D+ Y GG+F   + FPP+YP+ PP++ F ++++HP
Sbjct:   42 DTLYEGGLFKAKVAFPPEYPYAPPRLTFTSEMWHP 76
The following BLAST results are available for this feature:
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201
Match NameE-valueIdentityDescription
UBC4_WHEAT4.672e-1335.82Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum... [more]
UBE2S_RAT6.018e-1337.31Ubiquitin-conjugating enzyme E2 S OS=Rattus norveg... [more]
UBE2S_MOUSE6.018e-1337.31Ubiquitin-conjugating enzyme E2 S OS=Mus musculus ... [more]
UB2SA_XENLA6.018e-1337.31Ubiquitin-conjugating enzyme E2 S-A OS=Xenopus lae... [more]
UBE2S_DROPS6.032e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila ps... [more]
UBE2S_DROPE6.032e-1338.57Ubiquitin-conjugating enzyme E2 S OS=Drosophila pe... [more]
UBE2H_MOUSE6.070e-1332.35Ubiquitin-conjugating enzyme E2 H OS=Mus musculus ... [more]
UBE2H_HUMAN6.070e-1332.35Ubiquitin-conjugating enzyme E2 H OS=Homo sapiens ... [more]
UBE2H_BOVIN6.070e-1332.35Ubiquitin-conjugating enzyme E2 H OS=Bos taurus GN... [more]
UBC_ASFWA7.817e-1343.33Ubiquitin-conjugating enzyme E2 OS=African swine f... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA
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