BQ623021 | Citrus Genome Database

Overview


Name	BQ623021
Unique Name	BQ623021
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	526

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2225	contig	Csv1_Contig2225:326..849.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2E3_MOUSE (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 100.908 bits (250), Expect = 2.009e-30
Identity = 48/70 (68.57%), Postives = 57/70 (81.43%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:  137 NINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

HSP 2 Score: 51.6026 bits (122), Expect = 2.009e-30
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 1

Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFH 105
            S Y GGVF + I F  DYPFKPPKV FRT+++H
Sbjct:  103 SVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYH 135

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2E3_HUMAN (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.009e-30
Identity = 48/70 (68.57%), Postives = 57/70 (81.43%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:  137 NINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

HSP 2 Score: 51.6026 bits (122), Expect = 2.009e-30
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 1

Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFH 105
            S Y GGVF + I F  DYPFKPPKV FRT+++H
Sbjct:  103 SVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYH 135

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2E3_BOVIN (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.009e-30
Identity = 48/70 (68.57%), Postives = 57/70 (81.43%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y +++A+++  AR WT++YA
Sbjct:  137 NINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYA 206

HSP 2 Score: 51.6026 bits (122), Expect = 2.009e-30
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 1

Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFH 105
            S Y GGVF + I F  DYPFKPPKV FRT+++H
Sbjct:  103 SVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYH 135

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC31_ARATH (Probable ubiquitin carrier protein E2 31 OS=Arabidopsis thaliana GN=UBC31 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.937e-27
Identity = 38/70 (54.29%), Postives = 55/70 (78.57%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYA 319
            NIN  GSIC++ILK++W+PAL + KVLLSI  LL  PNPDDPLVPEI  ++K+++ +++  AR +T ++A
Sbjct:   83 NINDEGSICMNILKDKWTPALMVEKVLLSILLLLEKPNPDDPLVPEIGQLFKNNRFQFDQRAREFTARHA 152

HSP 2 Score: 54.299 bits (129), Expect = 1.937e-27
Identity = 20/34 (58.82%), Postives = 27/34 (79.41%), Query Frame = 1

Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +PY GG+F ++I FP DYPFKPPK  F+T ++HP
Sbjct:   49 TPYEGGMFNLSIKFPTDYPFKPPKFTFKTPIYHP 82

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2N_DROME (Ubiquitin-conjugating enzyme E2 N OS=Drosophila melanogaster GN=ben PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 4.925e-25
Identity = 39/71 (54.93%), Postives = 54/71 (76.06%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NI+  G ICLD+LK++WSPAL I  +LLSI +LL+ PNPDDPL  ++A ++K ++A+    AR WTQKYA+
Sbjct:   79 NIDRLGRICLDVLKDKWSPALQIRTILLSIQALLSAPNPDDPLANDVAELWKVNEAEAIRNAREWTQKYAV 149

HSP 2 Score: 48.9062 bits (115), Expect = 4.925e-25
Identity = 19/36 (52.78%), Postives = 25/36 (69.44%), Query Frame = 1

Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSP+ GGVF + +  P DYP   PKV F TK++HP
Sbjct:   43 NDSPFEGGVFKLELFLPEDYPMSAPKVRFITKIYHP 78

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2N_MACFA (Ubiquitin-conjugating enzyme E2 N OS=Macaca fascicularis GN=UBE2N PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 6.405e-25
Identity = 40/71 (56.34%), Postives = 56/71 (78.87%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            N++ +G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++A+   TAR+WT+ YAM
Sbjct:   79 NVDKSGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 45.8246 bits (107), Expect = 6.405e-25
Identity = 17/35 (48.57%), Postives = 23/35 (65.71%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSP+ GG F + +  P +YP   PKV F TK++HP
Sbjct:   44 DSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHP 78

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 8.336e-25
Identity = 41/71 (57.75%), Postives = 55/71 (77.46%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            N++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +KS++A+   TAR+WT+ YAM
Sbjct:   79 NVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIETARAWTRLYAM 149

HSP 2 Score: 45.8246 bits (107), Expect = 8.336e-25
Identity = 17/35 (48.57%), Postives = 23/35 (65.71%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSP+ GG F + +  P +YP   PKV F TK++HP
Sbjct:   44 DSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHP 78

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2N_PONAB (Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii GN=UBE2N PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.838e-24
Identity = 40/71 (56.34%), Postives = 55/71 (77.46%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            N++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++A+   TAR+WT+ YAM
Sbjct:   79 NVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 45.8246 bits (107), Expect = 1.838e-24
Identity = 17/35 (48.57%), Postives = 23/35 (65.71%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSP+ GG F + +  P +YP   PKV F TK++HP
Sbjct:   44 DSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHP 78

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2N_MOUSE (Ubiquitin-conjugating enzyme E2 N OS=Mus musculus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.838e-24
Identity = 40/71 (56.34%), Postives = 55/71 (77.46%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            N++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++A+   TAR+WT+ YAM
Sbjct:   79 NVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 45.8246 bits (107), Expect = 1.838e-24
Identity = 17/35 (48.57%), Postives = 23/35 (65.71%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSP+ GG F + +  P +YP   PKV F TK++HP
Sbjct:   44 DSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHP 78

[Close]

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2N_HUMAN (Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens GN=UBE2N PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.838e-24
Identity = 40/71 (56.34%), Postives = 55/71 (77.46%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            N++  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +K+++A+   TAR+WT+ YAM
Sbjct:   79 NVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAM 149

HSP 2 Score: 45.8246 bits (107), Expect = 1.838e-24
Identity = 17/35 (48.57%), Postives = 23/35 (65.71%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSP+ GG F + +  P +YP   PKV F TK++HP
Sbjct:   44 DSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHP 78

The following BLAST results are available for this feature:

BLAST of BQ623021 vs. ExPASy Swiss-Prot

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201


Match Name	E-value	Identity	Description
UB2E3_MOUSE	2.009e-30	68.57	Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus...	[more]
UB2E3_HUMAN	2.009e-30	68.57	Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens...	[more]
UB2E3_BOVIN	2.009e-30	68.57	Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus G...	[more]
UBC31_ARATH	1.937e-27	54.29	Probable ubiquitin carrier protein E2 31 OS=Arabid...	[more]
UBE2N_DROME	4.925e-25	54.93	Ubiquitin-conjugating enzyme E2 N OS=Drosophila me...	[more]
UBE2N_MACFA	6.405e-25	56.34	Ubiquitin-conjugating enzyme E2 N OS=Macaca fascic...	[more]
UBE2N_RAT	8.336e-25	57.75	Ubiquitin-conjugating enzyme E2 N OS=Rattus norveg...	[more]
UBE2N_PONAB	1.838e-24	56.34	Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii ...	[more]
UBE2N_MOUSE	1.838e-24	56.34	Ubiquitin-conjugating enzyme E2 N OS=Mus musculus ...	[more]
UBE2N_HUMAN	1.838e-24	56.34	Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens ...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA