BQ623021

Overview
NameBQ623021
Unique NameBQ623021
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length526
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2225 contig Csv1_Contig2225:326..849. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_ASHGO (Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossypii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.391e-16
Identity = 29/70 (41.43%), Postives = 43/70 (61.43%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY-----ESTARSW 304
            N+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K  K++Y     E+  +SW
Sbjct:   80 NVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSW 149

HSP 2 Score: 42.3578 bits (98), Expect = 1.391e-16
Identity = 16/36 (44.44%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+PY  G F + + F  +YP KPP V F +++FHP
Sbjct:   44 ADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_TRIHA (Ubiquitin-conjugating enzyme E2 2 OS=Trichoderma harzianum GN=UBC2 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.078e-16
Identity = 30/70 (42.86%), Postives = 44/70 (62.86%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY-----ESTARSW 304
            N+ +NG +CLDIL+ +WSP   ++ VL SI SLL DPN   P   E +++YK ++ +Y     E+  RSW
Sbjct:   80 NVYANGELCLDILQNRWSPTYDVAAVLTSIQSLLNDPNTGSPANVEASNLYKDNRREYIKRVRETVERSW 149

HSP 2 Score: 40.4318 bits (93), Expect = 3.078e-16
Identity = 15/36 (41.67%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+P+  G F + + F   YP KPP+V F +++FHP
Sbjct:   44 ADTPFEDGTFRLVMQFEEQYPNKPPQVKFISQMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_KLULA (Ubiquitin-conjugating enzyme E2 2 OS=Kluyveromyces lactis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 3.980e-16
Identity = 28/70 (40.00%), Postives = 43/70 (61.43%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY-----ESTARSW 304
            N+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A +++  K++Y     E+  +SW
Sbjct:   80 NVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFQDHKSQYVKRVKETVEKSW 149

HSP 2 Score: 42.3578 bits (98), Expect = 3.980e-16
Identity = 16/36 (44.44%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+PY  G F + + F  +YP KPP V F +++FHP
Sbjct:   44 ADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_USTMA (Ubiquitin-conjugating enzyme E2 2 OS=Ustilago maydis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 8.694e-16
Identity = 26/58 (44.83%), Postives = 39/58 (67.24%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY 283
            N+ +NG +CLDIL+ +WSP   ++ +L SI SLL DPNP+ P   E A +Y+ +  +Y
Sbjct:   80 NVYANGELCLDILQNRWSPTYDVAAILTSIQSLLHDPNPNSPANAEAASLYRENMKEY 137

HSP 2 Score: 41.2022 bits (95), Expect = 8.694e-16
Identity = 16/36 (44.44%), Postives = 23/36 (63.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+P+  G F + + F   YP KPP V F +K+FHP
Sbjct:   44 ADTPFEDGTFKLVLTFDESYPNKPPTVKFLSKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_DEBHA (Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces hansenii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 8.735e-16
Identity = 29/72 (40.28%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY-----ESTARSWTQ 310
            N+ ++G +CLDIL+ +WSP   +S +L SI SLL DPN   P   E A++YK  +++Y     E+   SW +
Sbjct:   80 NVYASGELCLDILQNRWSPTYDVSSILTSIQSLLNDPNISSPANVEAANLYKDHRSQYIKRVRETVENSWNE 151

HSP 2 Score: 40.0466 bits (92), Expect = 8.735e-16
Identity = 15/36 (41.67%), Postives = 23/36 (63.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+P+  G F + + F   YP KPP V F +++FHP
Sbjct:   44 ADTPFEDGTFRLILQFDEQYPNKPPSVKFISEMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC21_ARATH (Probable ubiquitin carrier protein E2 21 OS=Arabidopsis thaliana GN=UBC21 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.140e-15
Identity = 28/67 (41.79%), Postives = 40/67 (59.70%), Query Frame = 2 
Query:  125 GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKS-DKAKYESTARSWTQKYAM 322
            G ICLDILK  WSPA T+  V  +I +L+  P PD PL  +  ++ +S D   + S A+ +T+  AM
Sbjct:   87 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAM 153

HSP 2 Score: 48.9062 bits (115), Expect = 1.140e-15
Identity = 19/36 (52.78%), Postives = 25/36 (69.44%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            S++PY GGVF +    P  YP +PP+V F TK+FHP
Sbjct:   45 SETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHP 80
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_ASPFU (Ubiquitin-conjugating enzyme E2 2 OS=Aspergillus fumigatus GN=ubc2 PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.143e-15
Identity = 27/70 (38.57%), Postives = 42/70 (60.00%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY-----ESTARSW 304
            N+   G +CLDIL+ +WSP   ++ +L SI SLL DPN   P   E +++YK ++ +Y     E+  +SW
Sbjct:   80 NVYGTGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNTSSPANVEASNLYKDNRKEYIKRVRETVEKSW 149

HSP 2 Score: 42.3578 bits (98), Expect = 1.143e-15
Identity = 16/36 (44.44%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+P+  G F + +HF   YP KPP V F +++FHP
Sbjct:   44 ADTPFEDGTFRLVMHFEEQYPNKPPGVKFISQMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC1_CAEEL (Ubiquitin-conjugating enzyme E2 1 OS=Caenorhabditis elegans GN=ubc-1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.906e-15
Identity = 26/59 (44.07%), Postives = 41/59 (69.49%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N+ ++GSICLDIL+ +WSP   ++ +L SI SLL +PNP+ P     A +Y+ ++ +YE
Sbjct:   80 NVYADGSICLDILQNRWSPTYDVAAILTSIQSLLDEPNPNSPANSLAAQLYQENRREYE 138

HSP 2 Score: 40.817 bits (94), Expect = 1.906e-15
Identity = 15/35 (42.86%), Postives = 24/35 (68.57%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            ++P+  G F +++ F  +YP KPP V F +K+FHP
Sbjct:   45 ETPFEDGTFKLSLEFTEEYPNKPPTVKFISKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_CANAL (Ubiquitin-conjugating enzyme E2 2 OS=Candida albicans GN=UBC2 PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.912e-15
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY-----ESTARSW 304
            N+ ++G +CLDIL+ +WSP   +S +L S+ SLL DPN   P   E A++YK  ++ Y     E+   SW
Sbjct:   80 NVYASGELCLDILQNRWSPTYDVSSILTSVQSLLNDPNISSPANVEAANLYKDHRSLYVKRVRETVENSW 149

HSP 2 Score: 41.2022 bits (95), Expect = 1.912e-15
Identity = 16/36 (44.44%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            SD+P+  G F + + F   YP KPP+V F +++FHP
Sbjct:   44 SDTPFEDGTFRLLLSFDEQYPNKPPQVKFISEMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_NECHA (Ubiquitin-conjugating enzyme E2 2 OS=Nectria haematococca GN=UBC2 PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.930e-15
Identity = 27/68 (39.71%), Postives = 41/68 (60.29%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQK 313
            N+ + G +CLDIL+ +WSP   ++ VL SI SLL DPN   P   E +++YK ++ +Y    R   +K
Sbjct:   80 NVYATGELCLDILQNRWSPTYDVAAVLTSIQSLLNDPNTGSPANVEASNLYKDNRKEYTKRVRETVEK 147

HSP 2 Score: 40.4318 bits (93), Expect = 1.930e-15
Identity = 15/36 (41.67%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+P+  G F + + F   YP KPP+V F +++FHP
Sbjct:   44 ADTPFEDGTFRLVMQFEEQYPNKPPQVKFISEMFHP 79
The following BLAST results are available for this feature:
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201
Match NameE-valueIdentityDescription
UBC2_ASHGO1.391e-1641.43Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossyp... [more]
UBC2_TRIHA3.078e-1642.86Ubiquitin-conjugating enzyme E2 2 OS=Trichoderma h... [more]
UBC2_KLULA3.980e-1640.00Ubiquitin-conjugating enzyme E2 2 OS=Kluyveromyces... [more]
UBC2_USTMA8.694e-1644.83Ubiquitin-conjugating enzyme E2 2 OS=Ustilago mayd... [more]
UBC2_DEBHA8.735e-1640.28Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces ... [more]
UBC21_ARATH1.140e-1541.79Probable ubiquitin carrier protein E2 21 OS=Arabid... [more]
UBC2_ASPFU1.143e-1538.57Ubiquitin-conjugating enzyme E2 2 OS=Aspergillus f... [more]
UBC1_CAEEL1.906e-1544.07Ubiquitin-conjugating enzyme E2 1 OS=Caenorhabditi... [more]
UBC2_CANAL1.912e-1540.00Ubiquitin-conjugating enzyme E2 2 OS=Candida albic... [more]
UBC2_NECHA1.930e-1539.71Ubiquitin-conjugating enzyme E2 2 OS=Nectria haema... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA
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