BQ623186 | Citrus Genome Database

Overview


Name	BQ623186
Unique Name	BQ623186
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	610

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig12	contig	Csv1_Contig12:1..610.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: RAB8A_RAT (Ras-related protein Rab-8A OS=Rattus norvegicus GN=Rab8a PE=2 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 4.381e-12
Identity = 41/152 (26.97%), Postives = 80/152 (52.63%), Query Frame = 3

Query:  102 CVI-SYTSNTFPTDYVPTVFDNFSANVV-VDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPEL-RHYAPGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVF 548
            CV+  ++ + F + ++ T+  +F    + +DG  + L +WDTAGQE +  +    YRGA   +L + + ++ S++N+ + WI  +  H +  V  +++G K D+ D +Q          ++  +GE+L    G   ++E S+K   NV+  F
Sbjct:   23 CVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNI-RNWIRNIEEHASADVEKMILGNKCDVNDKRQ----------VSKERGEKLALDYGIK-FMETSAKANINVENAF 162

HSP 2 Score: 23.483 bits (49), Expect = 4.381e-12
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 2

Query:   59 KCVTVGDGAVGKTCM 103
            K + +GD  VGKTC+
Sbjct:   10 KLLLIGDSGVGKTCV 24

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: RAB8A_MOUSE (Ras-related protein Rab-8A OS=Mus musculus GN=Rab8a PE=1 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 4.381e-12
Identity = 41/152 (26.97%), Postives = 80/152 (52.63%), Query Frame = 3

Query:  102 CVI-SYTSNTFPTDYVPTVFDNFSANVV-VDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPEL-RHYAPGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVF 548
            CV+  ++ + F + ++ T+  +F    + +DG  + L +WDTAGQE +  +    YRGA   +L + + ++ S++N+ + WI  +  H +  V  +++G K D+ D +Q          ++  +GE+L    G   ++E S+K   NV+  F
Sbjct:   23 CVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNI-RNWIRNIEEHASADVEKMILGNKCDVNDKRQ----------VSKERGEKLALDYGIK-FMETSAKANINVENAF 162

HSP 2 Score: 23.483 bits (49), Expect = 4.381e-12
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 2

Query:   59 KCVTVGDGAVGKTCM 103
            K + +GD  VGKTC+
Sbjct:   10 KLLLIGDSGVGKTCV 24

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: RAB8A_MACFA (Ras-related protein Rab-8A OS=Macaca fascicularis GN=RAB8A PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.381e-12
Identity = 41/152 (26.97%), Postives = 80/152 (52.63%), Query Frame = 3

Query:  102 CVI-SYTSNTFPTDYVPTVFDNFSANVV-VDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPEL-RHYAPGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVF 548
            CV+  ++ + F + ++ T+  +F    + +DG  + L +WDTAGQE +  +    YRGA   +L + + ++ S++N+ + WI  +  H +  V  +++G K D+ D +Q          ++  +GE+L    G   ++E S+K   NV+  F
Sbjct:   23 CVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNI-RNWIRNIEEHASADVEKMILGNKCDVNDKRQ----------VSKERGEKLALDYGIK-FMETSAKANINVENAF 162

HSP 2 Score: 23.483 bits (49), Expect = 4.381e-12
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 2

Query:   59 KCVTVGDGAVGKTCM 103
            K + +GD  VGKTC+
Sbjct:   10 KLLLIGDSGVGKTCV 24

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: RAB8A_HUMAN (Ras-related protein Rab-8A OS=Homo sapiens GN=RAB8A PE=1 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.381e-12
Identity = 41/152 (26.97%), Postives = 80/152 (52.63%), Query Frame = 3

Query:  102 CVI-SYTSNTFPTDYVPTVFDNFSANVV-VDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPEL-RHYAPGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVF 548
            CV+  ++ + F + ++ T+  +F    + +DG  + L +WDTAGQE +  +    YRGA   +L + + ++ S++N+ + WI  +  H +  V  +++G K D+ D +Q          ++  +GE+L    G   ++E S+K   NV+  F
Sbjct:   23 CVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNI-RNWIRNIEEHASADVEKMILGNKCDVNDKRQ----------VSKERGEKLALDYGIK-FMETSAKANINVENAF 162

HSP 2 Score: 23.483 bits (49), Expect = 4.381e-12
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 2

Query:   59 KCVTVGDGAVGKTCM 103
            K + +GD  VGKTC+
Sbjct:   10 KLLLIGDSGVGKTCV 24

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: RAB8A_CANFA (Ras-related protein Rab-8A OS=Canis familiaris GN=RAB8A PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.381e-12
Identity = 41/152 (26.97%), Postives = 80/152 (52.63%), Query Frame = 3

Query:  102 CVI-SYTSNTFPTDYVPTVFDNFSANVV-VDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPEL-RHYAPGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVF 548
            CV+  ++ + F + ++ T+  +F    + +DG  + L +WDTAGQE +  +    YRGA   +L + + ++ S++N+ + WI  +  H +  V  +++G K D+ D +Q          ++  +GE+L    G   ++E S+K   NV+  F
Sbjct:   23 CVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNI-RNWIRNIEEHASADVEKMILGNKCDVNDKRQ----------VSKERGEKLALDYGIK-FMETSAKANINVENAF 162

HSP 2 Score: 23.483 bits (49), Expect = 4.381e-12
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 2

Query:   59 KCVTVGDGAVGKTCM 103
            K + +GD  VGKTC+
Sbjct:   10 KLLLIGDSGVGKTCV 24

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: RAB8A_BOVIN (Ras-related protein Rab-8A OS=Bos taurus GN=RAB8A PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.381e-12
Identity = 41/152 (26.97%), Postives = 80/152 (52.63%), Query Frame = 3

Query:  102 CVI-SYTSNTFPTDYVPTVFDNFSANVV-VDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPEL-RHYAPGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVF 548
            CV+  ++ + F + ++ T+  +F    + +DG  + L +WDTAGQE +  +    YRGA   +L + + ++ S++N+ + WI  +  H +  V  +++G K D+ D +Q          ++  +GE+L    G   ++E S+K   NV+  F
Sbjct:   23 CVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNI-RNWIRNIEEHASADVEKMILGNKCDVNDKRQ----------VSKERGEKLALDYGIK-FMETSAKANINVENAF 162

HSP 2 Score: 23.483 bits (49), Expect = 4.381e-12
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 2

Query:   59 KCVTVGDGAVGKTCM 103
            K + +GD  VGKTC+
Sbjct:   10 KLLLIGDSGVGKTCV 24

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: RAB13_MOUSE (Ras-related protein Rab-13 OS=Mus musculus GN=Rab13 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 4.386e-12
Identity = 43/160 (26.88%), Postives = 77/160 (48.12%), Query Frame = 3

Query:  105 VISYTSNTFPTDYVPTVFDNFSANVV-VDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPELRHYA-PGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAP--VYIECSSKTQQNVKAVFDAAIKVVL 572
            +I +  + F + Y+ T+  +F    V ++G  + L +WDTAGQE +  +    YRGA   +L + +  + S+EN+ + W+  ++  A  GV  +L+G K D+   +Q              Q E+  KL       + E S+K+  NV   F +  + +L
Sbjct:   25 IIRFAEDNFNSTYISTIGIDFKIRTVDIEGKRIKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFENI-QNWMKSIKENASAGVERLLLGNKCDMEAKRQ-------------VQREQAEKLAREHRIRFFETSAKSSVNVDEAFSSLARDIL 170

HSP 2 Score: 23.8682 bits (50), Expect = 4.386e-12
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 2

Query:   59 KCVTVGDGAVGKTCM 103
            K + +GD  VGKTC+
Sbjct:   10 KLLLIGDSGVGKTCL 24

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: YPT7_SCHPO (GTP-binding protein ypt7 OS=Schizosaccharomyces pombe GN=ypt7 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.473e-12
Identity = 52/160 (32.50%), Postives = 79/160 (49.38%), Query Frame = 3

Query:  114 YTSNTFPTDYVPTVFDNF-SANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPE-LRHYAPG----VPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVFDAAIKVVLQ 575
            Y +  F  DY  T+  +F +  V+VD   V L LWDTAGQE +  L    YRGAD  +L + + +  S+E +   W  E L   +P      P IL+G K+D+ + K+ ++    A+    A+GE          Y E S+K   NV+  F+   K+ L+
Sbjct:   28 YVNRKFSKDYKATIGADFLTKEVLVDDKVVTLQLWDTAGQERFQSLGVAFYRGADCCVLVYDVNNSKSFETL-DSWRDEFLIQASPSNPETFPFILLGNKVDVEEQKRMVSKSK-ALAFCQARGE--------IPYFETSAKEAINVQEAFETVAKLALE 177

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: YPT53_YEAST (GTP-binding protein YPT53 OS=Saccharomyces cerevisiae GN=YPT53 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.473e-12
Identity = 48/166 (28.92%), Postives = 78/166 (46.99%), Query Frame = 3

Query:  105 VISYTSNTFPTDYVPTVFDNF-SANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPELRH-YAPGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVFDAAIKVVLQPPKNTKK 596
            V+ + S+ F     PT+   F +  +  DG  +   +WDTAGQE +  L P+ YR A   L+ F + ++ S+   A+ W+ EL       + I LVG K+DL ++     +     P      E    L     Y E S+KT +N+  +F    + V  P +NT++
Sbjct:   29 VLRFVSDDFKESKEPTIGAAFLTKRITRDGKVIKFEIWDTAGQERFAPLAPMYYRNAQAALVVFDVTNEGSFYK-AQNWVEELHEKVGHDIVIALVGNKMDLLNNDDENENRAMKAPAVQNLCERENLL-----YFEASAKTGENIYQIFQTLGEKVPCPEQNTRQ 188

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Match: VPS21_YEAST (Vacuolar protein sorting-associated protein 21 OS=Saccharomyces cerevisiae GN=VPS21 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.473e-12
Identity = 49/158 (31.01%), Postives = 79/158 (50.00%), Query Frame = 3

Query:   81 APSARLACVISYTSNTFPTDYVPTVFDNF-SANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVAKKWIPELRHYA-PGVPIILVGTKLDLRDDKQFLADHPGAVPITTAQGEELRKLIGAPVYIECSSKTQQNVKAVF 548
            A   + + V+ + SN F  +  PT+   F +  V ++  TV   +WDTAGQE +  L P+ YR A   L+ + +    S+   A+ W+ EL   A   + I LVG K+D+  +        G   +   +GE+L +  G  ++ E S+KT +NV  VF
Sbjct:   16 AAVGKSSIVLRFVSNDFAENKEPTIGAAFLTQRVTINEHTVKFEIWDTAGQERFASLAPMYYRNAQAALVVYDVTKPQSFIK-ARHWVKELHEQASKDIIIALVGNKIDMLQE-------GGERKVAREEGEKLAEEKGL-LFFETSAKTGENVNDVF 164

The following BLAST results are available for this feature:

BLAST of BQ623186 vs. ExPASy Swiss-Prot

BLAST of BQ623186 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
RAB8A_RAT	4.381e-12	26.97	Ras-related protein Rab-8A OS=Rattus norvegicus GN...	[more]
RAB8A_MOUSE	4.381e-12	26.97	Ras-related protein Rab-8A OS=Mus musculus GN=Rab8...	[more]
RAB8A_MACFA	4.381e-12	26.97	Ras-related protein Rab-8A OS=Macaca fascicularis ...	[more]
RAB8A_HUMAN	4.381e-12	26.97	Ras-related protein Rab-8A OS=Homo sapiens GN=RAB8...	[more]
RAB8A_CANFA	4.381e-12	26.97	Ras-related protein Rab-8A OS=Canis familiaris GN=...	[more]
RAB8A_BOVIN	4.381e-12	26.97	Ras-related protein Rab-8A OS=Bos taurus GN=RAB8A ...	[more]
RAB13_MOUSE	4.386e-12	26.88	Ras-related protein Rab-13 OS=Mus musculus GN=Rab1...	[more]
YPT7_SCHPO	4.473e-12	32.50	GTP-binding protein ypt7 OS=Schizosaccharomyces po...	[more]
YPT53_YEAST	4.473e-12	28.92	GTP-binding protein YPT53 OS=Saccharomyces cerevis...	[more]
VPS21_YEAST	4.473e-12	31.01	Vacuolar protein sorting-associated protein 21 OS=...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_00277 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00277 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623186 ID=BQ623186; Name=BQ623186; organism=Citrus sinensis; type=EST; length=610bp
GTAATAAAGATACTGTCTTAAGTGCACGTGGCGCGAGATGAGTGCATCGA
GGTTCATAAAGTGCGTGACGGTCGGTGACGGCGCCGTCGGCAAGACTTGC
ATGCGTCATTTCTTACACCAGCAACACTTTCCCGACGGATTATGTGCCAA
CAGTATTTGACAATTTCAGTGCAAATGTGGTAGTGGATGGAAGCACTGTT
AATCTTGGGTTGTGGGACACTGCAGGCCAGGAAGATTACAATAGACTAAG
ACCATTGAGCTACCGCGGGGCAGATGTCTTCTTACTCGCATTCTCTCTCA
TTAGCAAGGCCAGCTATGAAAATGTTGCTAAGAAATGGATTCCTGAGTTG
AGGCATTATGCACCTGGTGTTCCAATAATTCTTGTCGGGACGAAGCTCGA
TCTTCGGGATGATAAGCAGTTCCTTGCAGATCACCCTGGTGCAGTGCCAA
TTACCACGGCCCAAGGGGAGGAATTGAGAAAGTTGATAGGTGCTCCTGTT
TACATTGAGTGTAGTTCCAAAACACAACAGAATGTGAAAGCTGTCTTTGA
TGCGGCCATCAAGGTGGTTCTCCAGCCGCCAAAGAACACGAAGAAAAAGA
AGAGAAGGCC