BQ623325

Overview
NameBQ623325
Unique NameBQ623325
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length512
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP1_SHEEP (Aquaporin-1 OS=Ovis aries GN=AQP1 PE=2 SV=3)

HSP 1 Score: 105.916 bits (263), Expect = 1.108e-22
Identity = 61/119 (51.26%), Postives = 74/119 (62.18%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G N LA G ++G GLG EIIGT  LV  V + TD +R  RD  +    PL IGF+V + HL  I  TG GINPARS G++VI      + D WIFWVGPFIGAA+A   + FIL   +S
Sbjct:  127 GLNALAPGVNSGQGLGIEIIGTLQLVLCVLATTD-RRRRRD--LGDSGPLAIGFSVALGHLLAIDYTGCGINPARSFGSSVITHN---FQDHWIFWVGPFIGAALAVLIYDFILAPRSS 239          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP1_PONAB (Aquaporin-1 OS=Pongo abelii GN=AQP1 PE=2 SV=3)

HSP 1 Score: 105.916 bits (263), Expect = 1.108e-22
Identity = 61/119 (51.26%), Postives = 75/119 (63.03%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G N+LADG ++G GLG EIIGT  LV  V + TD +R  RD  +   APL IG +V + HL  I  TG GINPARS G+AVI      + + WIFWVGPFIG A+A   + FIL   +S
Sbjct:  125 GRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRR--RD--LGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHN---FSNHWIFWVGPFIGGALAVLIYDFILAPRSS 236          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP1_PIG (Aquaporin-1 OS=Sus scrofa GN=AQP1 PE=2 SV=3)

HSP 1 Score: 105.916 bits (263), Expect = 1.108e-22
Identity = 59/119 (49.58%), Postives = 70/119 (58.82%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G N LA G  +G GLG EIIGT  LV  V + TD +R          APL IGF+V + HL  I  TG GINPARS G+AVI      + D W+FWVGPFIG A+A   + FIL   +S
Sbjct:  127 GLNSLAPGVDSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGS----APLAIGFSVALGHLLAIDYTGCGINPARSFGSAVITHN---FQDHWVFWVGPFIGGALAVLIYDFILAPRSS 238          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP1_HUMAN (Aquaporin-1 OS=Homo sapiens GN=AQP1 PE=1 SV=3)

HSP 1 Score: 105.916 bits (263), Expect = 1.108e-22
Identity = 61/119 (51.26%), Postives = 75/119 (63.03%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G N+LADG ++G GLG EIIGT  LV  V + TD +R  RD  +   APL IG +V + HL  I  TG GINPARS G+AVI      + + WIFWVGPFIG A+A   + FIL   +S
Sbjct:  125 GRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRR--RD--LGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHN---FSNHWIFWVGPFIGGALAVLIYDFILAPRSS 236          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP1_BOVIN (Aquaporin-1 OS=Bos taurus GN=AQP1 PE=1 SV=3)

HSP 1 Score: 105.145 bits (261), Expect = 1.889e-22
Identity = 59/119 (49.58%), Postives = 71/119 (59.66%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G N LA G ++G GLG EIIGT  LV  V + TD +R           PL IGF+V + HL  I  TG GINPARS G++VI      + D WIFWVGPFIGAA+A   + FIL   +S
Sbjct:  127 GLNALAPGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGS----GPLAIGFSVALGHLLAIDYTGCGINPARSFGSSVITHN---FQDHWIFWVGPFIGAALAVLIYDFILAPRSS 238          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP1_CANFA (Aquaporin-1 OS=Canis familiaris GN=AQP1 PE=2 SV=3)

HSP 1 Score: 103.99 bits (258), Expect = 4.209e-22
Identity = 61/119 (51.26%), Postives = 73/119 (61.34%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G NELA G ++G GLG EIIGT  LV  V + TD +R  RD  +    PL IG +V + HL  I  TG GINPARS G++VI    K   D WIFWVGPFIG A+A   + FIL   +S
Sbjct:  127 GRNELAPGVNSGQGLGIEIIGTLQLVLCVLATTDRRR--RD--LGGSGPLAIGLSVALGHLLAIDYTGCGINPARSFGSSVITHNFK---DHWIFWVGPFIGGALAVLIYDFILAPRSS 238          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP1_RAT (Aquaporin-1 OS=Rattus norvegicus GN=Aqp1 PE=2 SV=4)

HSP 1 Score: 103.219 bits (256), Expect = 7.179e-22
Identity = 57/119 (47.90%), Postives = 73/119 (61.34%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G N+LA G ++G GLG EIIGT  LV  V + TD +R          APL IG +V + HL  I  TG GINPARS G+AV+    + + + WIFWVGPFIG+A+A   + FIL   +S
Sbjct:  125 GRNDLARGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGS----APLAIGLSVALGHLLAIDYTGCGINPARSFGSAVL---TRNFSNHWIFWVGPFIGSALAVLIYDFILAPRSS 236          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP1_MOUSE (Aquaporin-1 OS=Mus musculus GN=Aqp1 PE=1 SV=3)

HSP 1 Score: 103.219 bits (256), Expect = 7.179e-22
Identity = 57/119 (47.90%), Postives = 72/119 (60.50%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G N+LA G ++G GLG EIIGT  LV  V + TD +R          APL IG +V + HL  I  TG GINPARS G+AV+    + + + WIFWVGPFIG A+A   + FIL   +S
Sbjct:  125 GRNDLAHGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGS----APLAIGLSVALGHLLAIDYTGCGINPARSFGSAVL---TRNFSNHWIFWVGPFIGGALAVLIYDFILAPRSS 236          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQPA_RANES (Aquaporin FA-CHIP OS=Rana esculenta GN=AQPA PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.497e-19
Identity = 56/119 (47.06%), Postives = 68/119 (57.14%), Query Frame = 2
Query:   71 GANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFILRASAS 427
            G N L+ G S G GLG EI+ TF LV  V + TD +R+     VP    L IG +V + HL  I  TG G+NPARS G+AV+    K +   WIFWVGP IG A AA  + FIL    S
Sbjct:  129 GLNGLSPGVSAGQGLGVEILVTFQLVLCVVAVTDRRRHDVSGSVP----LAIGLSVALGHLIAIDYTGCGMNPARSFGSAVL---TKNFTYHWIFWVGPMIGGAAAAIIYDFILAPRTS 240          
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Match: AQP4_RAT (Aquaporin-4 OS=Rattus norvegicus GN=Aqp4 PE=1 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 9.081e-17
Identity = 47/115 (40.87%), Postives = 66/115 (57.39%), Query Frame = 2
Query:   65 GGGANELADGYSTGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYSKEKAWDDQWIFWVGPFIGAAIAAFYHQFI 409
            G G   +    + G GL  E+I TF LV+T+F++ D KR    + V     L IGF+V + HL  I  TG  +NPARS G AVI      W++ WI+WVGP IGA +A   ++++
Sbjct:  144 GLGVTTVHGNLTAGHGLLVELIITFQLVFTIFASCDSKR----TDVTGSVALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGN---WENHWIYWVGPIIGAVLAGALYEYV 251          
The following BLAST results are available for this feature:
BLAST of BQ623325 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 132
Match NameE-valueIdentityDescription
AQP1_SHEEP1.108e-2251.26Aquaporin-1 OS=Ovis aries GN=AQP1 PE=2 SV=3[more]
AQP1_PONAB1.108e-2251.26Aquaporin-1 OS=Pongo abelii GN=AQP1 PE=2 SV=3[more]
AQP1_PIG1.108e-2249.58Aquaporin-1 OS=Sus scrofa GN=AQP1 PE=2 SV=3[more]
AQP1_HUMAN1.108e-2251.26Aquaporin-1 OS=Homo sapiens GN=AQP1 PE=1 SV=3[more]
AQP1_BOVIN1.889e-2249.58Aquaporin-1 OS=Bos taurus GN=AQP1 PE=1 SV=3[more]
AQP1_CANFA4.209e-2251.26Aquaporin-1 OS=Canis familiaris GN=AQP1 PE=2 SV=3[more]
AQP1_RAT7.179e-2247.90Aquaporin-1 OS=Rattus norvegicus GN=Aqp1 PE=2 SV=4[more]
AQP1_MOUSE7.179e-2247.90Aquaporin-1 OS=Mus musculus GN=Aqp1 PE=1 SV=3[more]
AQPA_RANES1.497e-1947.06Aquaporin FA-CHIP OS=Rana esculenta GN=AQPA PE=2 S... [more]
AQP4_RAT9.081e-1740.87Aquaporin-4 OS=Rattus norvegicus GN=Aqp4 PE=1 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00416 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00416 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623325 ID=BQ623325; Name=BQ623325; organism=Citrus sinensis; type=EST; length=512bp
TTGGGAGCCATATGTGGCTGCGGGCTCGTTAAGGCTTTCCAGAAGTCTTA
CTACATCGAGATACGGCGGTGGAGCCAATGAGCTCGCTGATGGGTACAGC
ACTGGCACTGGATTGGGTGCTGAGATCATCGGTACCTTTGTTCTTGTCTA
CACTGTTTTCTCCGCCACTGATCCCAAGAGAAATGCTAGGGACTCCCACG
TTCCCGTCTTGGCGCCATTGCCAATTGGATTTGCTGTGTTCATGGTTCAC
TTGGCTACGATCCCTGTCACTGGAACTGGCATCAACCCCGCTAGAAGTTT
GGGAGCTGCAGTGATTTACAGCAAGGAGAAGGCTTGGGATGATCAATGGA
TCTTCTGGGTTGGCCCCTTCATTGGTGCTGCAATTGCTGCATTTTACCAC
CAATTCATCCTCAGAGCATCTGCTTCTAAAGCTCTTGGATCCTTCAAAAG
CCTCCTCCAACATTTAAATTAACTTTTAAAATAATTACTGGAGGATTAAT
CAATGGGTGTCA
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