BQ623329

Overview
NameBQ623329
Unique NameBQ623329
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length588
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2850 contig Csv1_Contig2850:241..827. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_SOLLC (Eukaryotic translation initiation factor 5A-1 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 3.649e-48
Identity = 85/100 (85.00%), Postives = 90/100 (90.00%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            FV IDIF GKKLEDIVPSSHNCDVPHV RTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD+ LL+Q+KDGF  GKDLV+SV  AMGEEQI  +KDIGPK
Sbjct:   60 FVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDTLLAQVKDGFAEGKDLVLSVMSAMGEEQICGIKDIGPK 159

HSP 2 Score: 36.1946 bits (82), Expect = 3.649e-48
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   44 EVSTSKTGKHGHAKCH 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_TOBAC (Eukaryotic translation initiation factor 5A-1 OS=Nicotiana tabacum GN=EIF-5A1 PE=3 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 4.755e-48
Identity = 85/100 (85.00%), Postives = 90/100 (90.00%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            FV IDIF GKKLEDIVPSSHNCDVPHV RTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD+NLL+ IKDGF  GKDLV+SV  AMGEEQI  +KD+GPK
Sbjct:   60 FVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDNLLALIKDGFAEGKDLVLSVMSAMGEEQICGIKDVGPK 159

HSP 2 Score: 36.1946 bits (82), Expect = 4.755e-48
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   44 EVSTSKTGKHGHAKCH 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_NICPL (Eukaryotic translation initiation factor 5A-1 OS=Nicotiana plumbaginifolia GN=EIF-5A1 PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 4.755e-48
Identity = 85/100 (85.00%), Postives = 90/100 (90.00%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            FV IDIF GKKLEDIVPSSHNCDVPHV RTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD+NLL+ IKDGF  GKDLV+SV  AMGEEQI  +KD+GPK
Sbjct:   60 FVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDNLLALIKDGFAEGKDLVLSVMSAMGEEQICGIKDVGPK 159

HSP 2 Score: 36.1946 bits (82), Expect = 4.755e-48
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   44 EVSTSKTGKHGHAKCH 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_SOLTU (Eukaryotic translation initiation factor 5A-1/2 OS=Solanum tuberosum GN=EIF5A1 PE=2 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 6.198e-48
Identity = 86/100 (86.00%), Postives = 91/100 (91.00%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            FV IDIFNGKKLEDIVPSSHNCDVPHV RTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD+ LL+Q+K GFE GKDLV+SV  AMGEEQI A+KDIG K
Sbjct:   60 FVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDALLNQVKGGFEEGKDLVLSVMSAMGEEQICAVKDIGTK 159

HSP 2 Score: 36.1946 bits (82), Expect = 6.198e-48
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   44 EVSTSKTGKHGHAKCH 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A4_SOLLC (Eukaryotic translation initiation factor 5A-4 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 1.789e-47
Identity = 85/100 (85.00%), Postives = 90/100 (90.00%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            FV IDIFN KKLEDIVPSSHNCDVPHV RTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD+ LL+Q+K GFE GKDLV+SV  AMGEEQI A+KDIG K
Sbjct:   60 FVAIDIFNAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDTLLNQVKGGFEEGKDLVLSVMSAMGEEQICAVKDIGTK 159

HSP 2 Score: 36.1946 bits (82), Expect = 1.789e-47
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   44 EVSTSKTGKHGHAKCH 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_ARATH (Eukaryotic translation initiation factor 5A-1 OS=Arabidopsis thaliana GN=At1g13950 PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 5.162e-47
Identity = 83/100 (83.00%), Postives = 89/100 (89.00%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            FV IDIF  KKLEDIVPSSHNCDVPHV RTDYQLIDISEDG+VSLLT+NG+TKDDL+LP D+ LL QIK GF+ GKDLVVSV  AMGEEQINALKDIGPK
Sbjct:   59 FVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIKSGFDDGKDLVVSVMSAMGEEQINALKDIGPK 158

HSP 2 Score: 36.1946 bits (82), Expect = 5.162e-47
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   43 EVSTSKTGKHGHAKCH 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A3_ARATH (Eukaryotic translation initiation factor 5A-3 OS=Arabidopsis thaliana GN=At1g69410 PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 1.240e-45
Identity = 80/100 (80.00%), Postives = 90/100 (90.00%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            FV IDIF  KKLEDIVPSSHNCDVPHV R DYQLIDISEDGFVSLLT+NG+TKDDL+LPTDE LL+Q+K+GFE GKD+VVSV  AMGEEQ+ ALK++GPK
Sbjct:   59 FVAIDIFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLKNGFEEGKDIVVSVMSAMGEEQMCALKEVGPK 158

HSP 2 Score: 36.1946 bits (82), Expect = 1.240e-45
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   43 EVSTSKTGKHGHAKCH 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_MEDSA (Eukaryotic translation initiation factor 5A-1 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 2.105e-45
Identity = 84/101 (83.17%), Postives = 90/101 (89.11%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDI-VPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            FV IDIF  KKLE++ VPSSHNCDVPHV RTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD++LL+QIKDGF  GKDLVVSV  AMGEEQI ALKDIG K
Sbjct:   60 FVAIDIFTSKKLEEVYVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDSLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGGK 160

HSP 2 Score: 36.1946 bits (82), Expect = 2.105e-45
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   44 EVSTSKTGKHGHAKCH 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A_DIACA (Eukaryotic translation initiation factor 5A OS=Dianthus caryophyllus PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 2.976e-44
Identity = 75/97 (77.32%), Postives = 89/97 (91.75%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDI 342
            FV IDIFNGKKLEDIVPSSHNCDVPHV R DYQL+DI+EDGFVSLLT++G+TKDDL+LP DE L+ Q+K+GFE+GKDL++SV CAMGEEQI A+KD+
Sbjct:   60 FVAIDIFNGKKLEDIVPSSHNCDVPHVNRVDYQLLDITEDGFVSLLTDSGDTKDDLKLPADEALVKQMKEGFEAGKDLILSVMCAMGEEQICAVKDV 156

HSP 2 Score: 36.1946 bits (82), Expect = 2.976e-44
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   44 EVSTSKTGKHGHAKCH 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A2_ARATH (Eukaryotic translation initiation factor 5A-2 OS=Arabidopsis thaliana GN=At1g26630 PE=1 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 8.375e-41
Identity = 71/98 (72.45%), Postives = 85/98 (86.73%), Query Frame = 1 
Query:   52 FVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIG 345
            FV IDIF  KKLEDIVPSSHNCDVPHV R DYQLIDI+EDGFVSLLT++G TKDDL+LPTD+ L +Q++ GF+ GKD+VVSV  +MGEEQI A+K++G
Sbjct:   59 FVAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMRLGFDEGKDIVVSVMSSMGEEQICAVKEVG 156

HSP 2 Score: 36.1946 bits (82), Expect = 8.375e-41
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCH 50
            EVSTSKTGKHG AKCH
Sbjct:   43 EVSTSKTGKHGHAKCH 58
The following BLAST results are available for this feature:
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 43
Match NameE-valueIdentityDescription
IF5A1_SOLLC3.649e-4885.00Eukaryotic translation initiation factor 5A-1 OS=S... [more]
IF5A1_TOBAC4.755e-4885.00Eukaryotic translation initiation factor 5A-1 OS=N... [more]
IF5A1_NICPL4.755e-4885.00Eukaryotic translation initiation factor 5A-1 OS=N... [more]
IF5A1_SOLTU6.198e-4886.00Eukaryotic translation initiation factor 5A-1/2 OS... [more]
IF5A4_SOLLC1.789e-4785.00Eukaryotic translation initiation factor 5A-4 OS=S... [more]
IF5A1_ARATH5.162e-4783.00Eukaryotic translation initiation factor 5A-1 OS=A... [more]
IF5A3_ARATH1.240e-4580.00Eukaryotic translation initiation factor 5A-3 OS=A... [more]
IF5A1_MEDSA2.105e-4583.17Eukaryotic translation initiation factor 5A-1 OS=M... [more]
IF5A_DIACA2.976e-4477.32Eukaryotic translation initiation factor 5A OS=Dia... [more]
IF5A2_ARATH8.375e-4172.45Eukaryotic translation initiation factor 5A-2 OS=A... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00420 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00420 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623329 ID=BQ623329; Name=BQ623329; organism=Citrus sinensis; type=EST; length=588bp
GGGAGGTTTCAACTTCCAAGACTGGAAAGCACGGTGATGCTAAGTGTCAC
CTTTGTAGGAATTGACATCTTCAATGGCAAGAAGCTTGAGGATATTGTTC
CCTCTTCCCACAACTGTGATGTTCCTCATGTCACTCGCACTGACTACCAG
CTGATTGATATCTCTGAGGATGGATTTGTTAGCCTGCTGACTGAGAATGG
CAATACCAAGGATGACCTGAGACTTCCAACTGATGAAAATCTGCTTTCAC
AGATCAAGGATGGATTTGAGAGTGGGAAAGATCTGGTTGTCTCAGTTCAG
TGTGCTATGGGAGAGGAGCAGATCAATGCCCTGAAGGACATTGGCCCCAA
GTAAAAGACTTTTTGCTTGATCGAGAATGCAGATTGCTGAAAGCTGTGGG
AAGGTGGATAGTAGTGTAGCTTACTGGTGTCCGGTTTCAAGATGAATGTC
ATCATTTCATATAAAATGACTCTTACCATATTATTAATTGTTTTCTATCG
ACAATATTTTTATTCTGTTTGATTGCTTGAAAGTTGAAACATGGTTGTTC
TCGGGGTGCAGAAGTTGTATCCGCCAACGTGACTGTAT
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