BQ623329

Overview
NameBQ623329
Unique NameBQ623329
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length588
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A_SPOFR (Eukaryotic translation initiation factor 5A OS=Spodoptera frugiperda GN=eIF-5A PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 1.019e-27
Identity = 53/97 (54.64%), Postives = 76/97 (78.35%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIFNGKK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ +NG+ ++DL++P D +L +Q++  F+SGK+L+ +V  + GEE + A+K
Sbjct:   58 VHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLRSDFDSGKELLCTVLKSCGEECVIAVK 153

HSP 2 Score: 32.7278 bits (73), Expect = 1.019e-27
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   44 EMSTSKTGKHGHAKVHL 60
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A_SPOEX (Eukaryotic translation initiation factor 5A OS=Spodoptera exigua GN=eIF-5A PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 1.019e-27
Identity = 53/97 (54.64%), Postives = 76/97 (78.35%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIFNGKK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ +NG+ ++DL++P D +L +Q++  F+SGK+L+ +V  + GEE + A+K
Sbjct:   58 VHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLRSDFDSGKELLCTVLKSCGEECVIAVK 153

HSP 2 Score: 32.7278 bits (73), Expect = 1.019e-27
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   44 EMSTSKTGKHGHAKVHL 60
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A2_SCHPO (Eukaryotic translation initiation factor 5A-2 OS=Schizosaccharomyces pombe GN=tif51b PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.529e-25
Identity = 53/98 (54.08%), Postives = 75/98 (76.53%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKD 339
            V  V +DIFNG+K ED+ PS+HN DVP V R +YQL++I +DG+++L+T +G TKDD+RLP  E L ++I++GFE GKDL+++V  AMGEE   A +D
Sbjct:   58 VHIVALDIFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGE-LGNEIEEGFEEGKDLIITVVSAMGEEIALACRD 153

HSP 2 Score: 30.8018 bits (68), Expect = 1.529e-25
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            ++STSKTGKHG AK H+
Sbjct:   44 DMSTSKTGKHGHAKVHI 60
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A2_PONAB (Eukaryotic translation initiation factor 5A-2 OS=Pongo abelii GN=EIF5A2 PE=2 SV=3)

HSP 1 Score: 103.219 bits (256), Expect = 2.593e-25
Identity = 54/97 (55.67%), Postives = 70/97 (72.16%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R DYQLI I +DG++SLLTE G  ++DL+LP  E L  +I+  + +G+D+ VSV CAM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICI-QDGYLSLLTETGEVREDLKLPEGE-LGKEIEGKYNAGEDVQVSVMCAMSEEYAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 2.593e-25
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A2_MOUSE (Eukaryotic translation initiation factor 5A-2 OS=Mus musculus GN=Eif5a2 PE=2 SV=3)

HSP 1 Score: 103.219 bits (256), Expect = 2.593e-25
Identity = 54/97 (55.67%), Postives = 70/97 (72.16%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R DYQLI I +DG++SLLTE G  ++DL+LP  E L  +I+  + +G+D+ VSV CAM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICI-QDGYLSLLTETGEVREDLKLPEGE-LGKEIEGKYNAGEDVQVSVMCAMSEEYAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 2.593e-25
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A2_HUMAN (Eukaryotic translation initiation factor 5A-2 OS=Homo sapiens GN=EIF5A2 PE=1 SV=3)

HSP 1 Score: 103.219 bits (256), Expect = 2.593e-25
Identity = 54/97 (55.67%), Postives = 70/97 (72.16%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R DYQLI I +DG++SLLTE G  ++DL+LP  E L  +I+  + +G+D+ VSV CAM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICI-QDGYLSLLTETGEVREDLKLPEGE-LGKEIEGKYNAGEDVQVSVMCAMSEEYAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 2.593e-25
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_SCHPO (Eukaryotic translation initiation factor 5A-1 OS=Schizosaccharomyces pombe GN=tif51a PE=1 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 4.390e-25
Identity = 51/98 (52.04%), Postives = 75/98 (76.53%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKD 339
            V  V +DIFNG+K ED+ PS+HN DVP V R +YQL++I +DG+++L+T +G TKDD+RLP  E L ++I++GF+ G+DL+++V  AMGEE   A +D
Sbjct:   58 VHIVALDIFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGE-LGNEIEEGFDEGRDLIITVVSAMGEETALACRD 153

HSP 2 Score: 30.8018 bits (68), Expect = 4.390e-25
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            ++STSKTGKHG AK H+
Sbjct:   44 DMSTSKTGKHGHAKVHI 60
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A_DROME (Eukaryotic translation initiation factor 5A OS=Drosophila melanogaster GN=eIF-5A PE=1 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 3.611e-24
Identity = 52/97 (53.61%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF+ KK EDI PS+HN DVP+V R D QLI IS+D F++L+TE+G+ ++DL++P  E L  Q++  F+SGKDL+ +V  A GEE + A+K
Sbjct:   57 VHMVGIDIFSNKKYEDICPSTHNMDVPNVKREDLQLIAISDDSFLTLMTESGDLREDLKVPEGE-LGEQLRLDFDSGKDLLCTVLKACGEECVIAIK 152

HSP 2 Score: 31.9574 bits (71), Expect = 3.611e-24
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK H+
Sbjct:   43 EMSTSKTGKHGHAKVHM 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A_CANAL (Eukaryotic translation initiation factor 5A OS=Candida albicans GN=ANB1 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 4.704e-24
Identity = 49/98 (50.00%), Postives = 75/98 (76.53%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKD 339
            V  V IDIF GKKLED+ PS+HN +VP+V+R ++QL+DI +DG++SL+T +G+TKDD+++P  E L  +++  F+ GKDL+V++  AMGEE   + K+
Sbjct:   56 VHLVAIDIFTGKKLEDLSPSTHNMEVPNVSRQEFQLLDI-DDGYLSLMTADGDTKDDVKVPEGE-LGDKLQSEFDEGKDLIVTIISAMGEEAAISYKE 151

HSP 2 Score: 31.5722 bits (70), Expect = 4.704e-24
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            ++STSKTGKHG AK HL
Sbjct:   42 DMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A2_CHICK (Eukaryotic translation initiation factor 5A-2 OS=Gallus gallus GN=EIF5A2 PE=1 SV=3)

HSP 1 Score: 98.5969 bits (244), Expect = 6.128e-24
Identity = 52/97 (53.61%), Postives = 71/97 (73.20%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIFNGKK EDI PS+HN DVP++ R DYQLI I +DG++SLLTE+G  ++DL+LP + +L  +I+  F + +D+ +SV  AM EE   A+K
Sbjct:   56 VHLVGIDIFNGKKYEDICPSTHNMDVPNIKRNDYQLIGI-QDGYLSLLTESGEVREDLKLP-EGDLGKEIEGKFNANEDVQISVISAMNEECAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 6.128e-24
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
The following BLAST results are available for this feature:
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 43
Match NameE-valueIdentityDescription
IF5A_SPOFR1.019e-2754.64Eukaryotic translation initiation factor 5A OS=Spo... [more]
IF5A_SPOEX1.019e-2754.64Eukaryotic translation initiation factor 5A OS=Spo... [more]
IF5A2_SCHPO1.529e-2554.08Eukaryotic translation initiation factor 5A-2 OS=S... [more]
IF5A2_PONAB2.593e-2555.67Eukaryotic translation initiation factor 5A-2 OS=P... [more]
IF5A2_MOUSE2.593e-2555.67Eukaryotic translation initiation factor 5A-2 OS=M... [more]
IF5A2_HUMAN2.593e-2555.67Eukaryotic translation initiation factor 5A-2 OS=H... [more]
IF5A1_SCHPO4.390e-2552.04Eukaryotic translation initiation factor 5A-1 OS=S... [more]
IF5A_DROME3.611e-2453.61Eukaryotic translation initiation factor 5A OS=Dro... [more]
IF5A_CANAL4.704e-2450.00Eukaryotic translation initiation factor 5A OS=Can... [more]
IF5A2_CHICK6.128e-2453.61Eukaryotic translation initiation factor 5A-2 OS=G... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00420 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00420 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623329 ID=BQ623329; Name=BQ623329; organism=Citrus sinensis; type=EST; length=588bp
GGGAGGTTTCAACTTCCAAGACTGGAAAGCACGGTGATGCTAAGTGTCAC
CTTTGTAGGAATTGACATCTTCAATGGCAAGAAGCTTGAGGATATTGTTC
CCTCTTCCCACAACTGTGATGTTCCTCATGTCACTCGCACTGACTACCAG
CTGATTGATATCTCTGAGGATGGATTTGTTAGCCTGCTGACTGAGAATGG
CAATACCAAGGATGACCTGAGACTTCCAACTGATGAAAATCTGCTTTCAC
AGATCAAGGATGGATTTGAGAGTGGGAAAGATCTGGTTGTCTCAGTTCAG
TGTGCTATGGGAGAGGAGCAGATCAATGCCCTGAAGGACATTGGCCCCAA
GTAAAAGACTTTTTGCTTGATCGAGAATGCAGATTGCTGAAAGCTGTGGG
AAGGTGGATAGTAGTGTAGCTTACTGGTGTCCGGTTTCAAGATGAATGTC
ATCATTTCATATAAAATGACTCTTACCATATTATTAATTGTTTTCTATCG
ACAATATTTTTATTCTGTTTGATTGCTTGAAAGTTGAAACATGGTTGTTC
TCGGGGTGCAGAAGTTGTATCCGCCAACGTGACTGTAT
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