BQ623329

Overview
NameBQ623329
Unique NameBQ623329
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length588
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A2_YEAST (Eukaryotic translation initiation factor 5A-2 OS=Saccharomyces cerevisiae GN=HYP2 PE=1 SV=3)

HSP 1 Score: 97.4413 bits (241), Expect = 2.974e-23
Identity = 50/98 (51.02%), Postives = 70/98 (71.43%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKD 339
            V  V IDIF GKKLED+ PS+HN +VP V R +YQL+DI +DGF+SL+  +G+TKDD++ P  E L   ++  F+ GKDL+V++  AMGEE   + K+
Sbjct:   57 VHLVAIDIFTGKKLEDLSPSTHNMEVPVVKRNEYQLLDI-DDGFLSLMNMDGDTKDDVKAPEGE-LGDSLQTAFDEGKDLMVTIISAMGEEAAISFKE 152

HSP 2 Score: 31.5722 bits (70), Expect = 2.974e-23
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            ++STSKTGKHG AK HL
Sbjct:   43 DMSTSKTGKHGHAKVHL 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_YEAST (Eukaryotic translation initiation factor 5A-1 OS=Saccharomyces cerevisiae GN=HYP1 PE=1 SV=3)

HSP 1 Score: 96.6709 bits (239), Expect = 5.036e-23
Identity = 49/98 (50.00%), Postives = 71/98 (72.45%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKD 339
            V  V +DIF GKKLED+ PS+HN +VP V R++YQL+DI +DG++SL+T +G TKDD++ P  E L   ++  F+ GKDL+V++  AMGEE   + K+
Sbjct:   57 VHLVTLDIFTGKKLEDLSPSTHNLEVPFVKRSEYQLLDI-DDGYLSLMTMDGETKDDVKAPEGE-LGDSMQAAFDEGKDLMVTIISAMGEEAAISFKE 152

HSP 2 Score: 31.5722 bits (70), Expect = 5.036e-23
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            ++STSKTGKHG AK HL
Sbjct:   43 DMSTSKTGKHGHAKVHL 59
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_RABIT (Eukaryotic translation initiation factor 5A-1 OS=Oryctolagus cuniculus GN=EIF5A PE=1 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 6.559e-23
Identity = 48/97 (49.48%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R D+QLI I +DG++SLL ++G  ++DLRLP + +L  +I+  ++SG++++++V  AM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGI-QDGYLSLLQDSGEVREDLRLP-EGDLGKEIEQKYDSGEEILITVLSAMTEEAAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 6.559e-23
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_RAT (Eukaryotic translation initiation factor 5A-1 OS=Rattus norvegicus GN=Eif5a PE=1 SV=3)

HSP 1 Score: 93.2041 bits (230), Expect = 2.447e-22
Identity = 47/97 (48.45%), Postives = 71/97 (73.20%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R D+QLI I +DG++SLL ++G  ++DLRLP + +L  +I+  ++ G++++++V  AM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGI-QDGYLSLLQDSGEVREDLRLP-EGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 2.447e-22
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_MOUSE (Eukaryotic translation initiation factor 5A-1 OS=Mus musculus GN=Eif5a PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 2.447e-22
Identity = 47/97 (48.45%), Postives = 71/97 (73.20%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R D+QLI I +DG++SLL ++G  ++DLRLP + +L  +I+  ++ G++++++V  AM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGI-QDGYLSLLQDSGEVREDLRLP-EGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 2.447e-22
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_HUMAN (Eukaryotic translation initiation factor 5A-1 OS=Homo sapiens GN=EIF5A PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 2.447e-22
Identity = 47/97 (48.45%), Postives = 71/97 (73.20%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R D+QLI I +DG++SLL ++G  ++DLRLP + +L  +I+  ++ G++++++V  AM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGI-QDGYLSLLQDSGEVREDLRLP-EGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 2.447e-22
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_BOVIN (Eukaryotic translation initiation factor 5A-1 OS=Bos taurus GN=EIF5A PE=2 SV=3)

HSP 1 Score: 93.2041 bits (230), Expect = 2.447e-22
Identity = 47/97 (48.45%), Postives = 71/97 (73.20%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R D+QLI I +DG++SLL ++G  ++DLRLP + +L  +I+  ++ G++++++V  AM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGI-QDGYLSLLQDSGEVREDLRLP-EGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150

HSP 2 Score: 32.7278 bits (73), Expect = 2.447e-22
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A_NEUCR (Eukaryotic translation initiation factor 5A OS=Neurospora crassa GN=tif-51 PE=2 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 2.004e-21
Identity = 52/102 (50.98%), Postives = 64/102 (62.75%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALKDIGPK 351
            V  V IDIF GKKLED+ PS+HN DVP+V RTDYQ   I ED F+ L+  NG  K D+++P  E L  +I+   E GKD  V VQ AMGEE    +K+   K
Sbjct:   63 VHLVAIDIFTGKKLEDLCPSTHNMDVPNVKRTDYQFSYIDED-FLVLIDSNGEEKRDVKMPEGE-LAKRIEKLEEEGKDFFVGVQTAMGEEAAIDVKEASNK 162

HSP 2 Score: 31.5722 bits (70), Expect = 2.004e-21
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            ++STSKTGKHG AK HL
Sbjct:   49 DMSTSKTGKHGHAKVHL 65
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5AL_HUMAN (Eukaryotic translation initiation factor 5A-1-like OS=Homo sapiens GN=EIF5AL1 PE=1 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 2.010e-21
Identity = 47/97 (48.45%), Postives = 70/97 (72.16%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLVVSVQCAMGEEQINALK 336
            V  VGIDIF GKK EDI PS+HN DVP++ R D+QLI I +DG++SLL ++G   +DLRLP + +L  +I+  ++ G++++++V  AM EE   A+K
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGI-QDGYLSLLQDSGEVPEDLRLP-EGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150

HSP 2 Score: 30.8018 bits (68), Expect = 2.010e-21
Identity = 13/17 (76.47%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+S SKTGKHG AK HL
Sbjct:   42 EMSASKTGKHGHAKVHL 58
[Close]
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Match: IF5A1_CHICK (Eukaryotic translation initiation factor 5A-1 OS=Gallus gallus GN=EIF5A1 PE=1 SV=3)

HSP 1 Score: 84.3445 bits (207), Expect = 1.043e-19
Identity = 42/81 (51.85%), Postives = 60/81 (74.07%), Query Frame = 1 
Query:   46 VTFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDLV 288
            V  VGIDIF GKK EDI PS+HN DVP++ R D+QLI I +DGF+SLL ++G  ++DLRLP  E L  +I+  ++ G++++
Sbjct:   56 VHLVGIDIFTGKKYEDICPSTHNMDVPNIKRCDFQLIGI-QDGFLSLLQDSGEVREDLRLPEGE-LGREIEQKYDCGEEII 134

HSP 2 Score: 32.7278 bits (73), Expect = 1.043e-19
Identity = 14/17 (82.35%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:    3 EVSTSKTGKHGDAKCHL 53
            E+STSKTGKHG AK HL
Sbjct:   42 EMSTSKTGKHGHAKVHL 58
The following BLAST results are available for this feature:
BLAST of BQ623329 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 43
Match NameE-valueIdentityDescription
IF5A2_YEAST2.974e-2351.02Eukaryotic translation initiation factor 5A-2 OS=S... [more]
IF5A1_YEAST5.036e-2350.00Eukaryotic translation initiation factor 5A-1 OS=S... [more]
IF5A1_RABIT6.559e-2349.48Eukaryotic translation initiation factor 5A-1 OS=O... [more]
IF5A1_RAT2.447e-2248.45Eukaryotic translation initiation factor 5A-1 OS=R... [more]
IF5A1_MOUSE2.447e-2248.45Eukaryotic translation initiation factor 5A-1 OS=M... [more]
IF5A1_HUMAN2.447e-2248.45Eukaryotic translation initiation factor 5A-1 OS=H... [more]
IF5A1_BOVIN2.447e-2248.45Eukaryotic translation initiation factor 5A-1 OS=B... [more]
IF5A_NEUCR2.004e-2150.98Eukaryotic translation initiation factor 5A OS=Neu... [more]
IF5AL_HUMAN2.010e-2148.45Eukaryotic translation initiation factor 5A-1-like... [more]
IF5A1_CHICK1.043e-1951.85Eukaryotic translation initiation factor 5A-1 OS=G... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00420 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00420 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623329 ID=BQ623329; Name=BQ623329; organism=Citrus sinensis; type=EST; length=588bp
GGGAGGTTTCAACTTCCAAGACTGGAAAGCACGGTGATGCTAAGTGTCAC
CTTTGTAGGAATTGACATCTTCAATGGCAAGAAGCTTGAGGATATTGTTC
CCTCTTCCCACAACTGTGATGTTCCTCATGTCACTCGCACTGACTACCAG
CTGATTGATATCTCTGAGGATGGATTTGTTAGCCTGCTGACTGAGAATGG
CAATACCAAGGATGACCTGAGACTTCCAACTGATGAAAATCTGCTTTCAC
AGATCAAGGATGGATTTGAGAGTGGGAAAGATCTGGTTGTCTCAGTTCAG
TGTGCTATGGGAGAGGAGCAGATCAATGCCCTGAAGGACATTGGCCCCAA
GTAAAAGACTTTTTGCTTGATCGAGAATGCAGATTGCTGAAAGCTGTGGG
AAGGTGGATAGTAGTGTAGCTTACTGGTGTCCGGTTTCAAGATGAATGTC
ATCATTTCATATAAAATGACTCTTACCATATTATTAATTGTTTTCTATCG
ACAATATTTTTATTCTGTTTGATTGCTTGAAAGTTGAAACATGGTTGTTC
TCGGGGTGCAGAAGTTGTATCCGCCAACGTGACTGTAT
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