BQ623442 | Citrus Genome Database

Overview


Name	BQ623442
Unique Name	BQ623442
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	449

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig12425	contig	Csv1_Contig12425:337..783.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS_CHLMO (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Chlamydomonas moewusii GN=RBCS PE=2 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 8.750e-22
Identity = 37/63 (58.73%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  105 SPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 293
            S GYYD RYWTMWKLPM+GCTD +QVL+EV   Q  +P+ ++R++ FDN +QVQC+ FL  +P
Sbjct:   92 SAGYYDNRYWTMWKLPMFGCTDPSQVLREVSACQVAFPNVYIRLVAFDNVKQVQCMGFLVQRP 154

HSP 2 Score: 30.0314 bits (66), Expect = 8.750e-22
Identity = 8/17 (47.06%), Postives = 14/17 (82.35%), Query Frame = 2

Query:   14 KEISYLLRSGWIPCLEF 64
            +++ Y++ +GW PCLEF
Sbjct:   56 RQVDYIINNGWTPCLEF 72

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS1_CHLRE (Ribulose bisphosphate carboxylase small chain 1, chloroplastic OS=Chlamydomonas reinhardtii GN=RBCS-1 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.508e-20
Identity = 33/60 (55.00%), Postives = 45/60 (75.00%), Query Frame = 3

Query:  114 YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 293
            YYD RYWTMWKLPM+GC D  QVL+E+    K +P ++VR++ FDN++QVQ + FL  +P
Sbjct:  112 YYDNRYWTMWKLPMFGCRDPMQVLREIVACTKAFPDAYVRLVAFDNQKQVQIMGFLVQRP 171

HSP 2 Score: 33.8834 bits (76), Expect = 4.508e-20
Identity = 13/29 (44.83%), Postives = 21/29 (72.41%), Query Frame = 2

Query:    2 EALLKEISYLLRSGWIPCLEF-ELEKGWV 85
            E +  ++ Y++ +GWIPCLEF E +K +V
Sbjct:   69 EQIAAQVDYIVANGWIPCLEFAEADKAYV 97

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS2_CHLRE (Ribulose bisphosphate carboxylase small chain 2, chloroplastic OS=Chlamydomonas reinhardtii GN=RBCS-2 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 5.864e-20
Identity = 33/60 (55.00%), Postives = 45/60 (75.00%), Query Frame = 3

Query:  114 YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 293
            YYD RYWTMWKLPM+GC D  QVL+E+    K +P ++VR++ FDN++QVQ + FL  +P
Sbjct:  112 YYDNRYWTMWKLPMFGCRDPMQVLREIVACTKAFPDAYVRLVAFDNQKQVQIMGFLVQRP 171

HSP 2 Score: 33.4982 bits (75), Expect = 5.864e-20
Identity = 13/29 (44.83%), Postives = 21/29 (72.41%), Query Frame = 2

Query:    2 EALLKEISYLLRSGWIPCLEF-ELEKGWV 85
            E +  ++ Y++ +GWIPCLEF E +K +V
Sbjct:   69 EQIAAQVDYIVANGWIPCLEFAESDKAYV 97

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS_EUGGR (Ribulose bisphosphate carboxylase small chains, chloroplastic OS=Euglena gracilis GN=RBCS1 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.068e-19
Identity = 44/90 (48.89%), Postives = 59/90 (65.56%), Query Frame = 3

Query:   30 FSAPAGFPAWNSSWRKDGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPPG 299
            F+AP      NS    D       ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  FD+ +QVQ ISF+  +P G
Sbjct:  178 FAAPE-----NSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISFVVQRPSG 262

HSP 2 Score: 95.5153 bits (236), Expect = 1.068e-19
Identity = 44/90 (48.89%), Postives = 59/90 (65.56%), Query Frame = 3

Query:   30 FSAPAGFPAWNSSWRKDGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPPG 299
            F+AP      NS    D       ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  FD+ +QVQ ISF+  +P G
Sbjct:  322 FAAPE-----NSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISFVVQRPSG 406

HSP 3 Score: 95.5153 bits (236), Expect = 1.068e-19
Identity = 44/90 (48.89%), Postives = 59/90 (65.56%), Query Frame = 3

Query:   30 FSAPAGFPAWNSSWRKDGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPPG 299
            F+AP      NS    D       ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  FD+ +QVQ ISF+  +P G
Sbjct:  609 FAAPE-----NSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISFVVQRPSG 693

HSP 4 Score: 95.5153 bits (236), Expect = 1.068e-19
Identity = 44/90 (48.89%), Postives = 59/90 (65.56%), Query Frame = 3

Query:   30 FSAPAGFPAWNSSWRKDGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPPG 299
            F+AP      NS    D       ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  FD+ +QVQ ISF+  +P G
Sbjct:  752 FAAPE-----NSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISFVVQRPSG 836

HSP 5 Score: 95.5153 bits (236), Expect = 1.068e-19
Identity = 44/90 (48.89%), Postives = 59/90 (65.56%), Query Frame = 3

Query:   30 FSAPAGFPAWNSSWRKDGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPPG 299
            F+AP      NS    D       ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  FD+ +QVQ ISF+  +P G
Sbjct: 1040 FAAPE-----NSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISFVVQRPSG 1124

HSP 6 Score: 93.5893 bits (231), Expect = 4.059e-19
Identity = 43/90 (47.78%), Postives = 58/90 (64.44%), Query Frame = 3

Query:   30 FSAPAGFPAWNSSWRKDGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPPG 299
            F+AP      NS    D       ++ GYYD  YWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  FD+ +QVQ ISF+  +P G
Sbjct:  465 FAAPE-----NSFIANDNTVRFSGTAAGYYDNHYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISFVVQRPSG 549

HSP 7 Score: 93.5893 bits (231), Expect = 4.059e-19
Identity = 43/88 (48.86%), Postives = 58/88 (65.91%), Query Frame = 3

Query:   30 FSAPAGFPAWNSSWRKDGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 293
            F+AP      NS    D       ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR+  FD+ +QVQ ISF+  +P
Sbjct: 1184 FAAPE-----NSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVRLAAFDSVKQVQVISFVVQRP 1266

HSP 8 Score: 91.2781 bits (225), Expect = 2.015e-18
Identity = 44/90 (48.89%), Postives = 59/90 (65.56%), Query Frame = 3

Query:   30 FSAPAGFPAWNSSWRKDGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPPG 299
            F+AP      NS    D       ++ GYYD RYWTMWKLPM+GCTDA+QVL+E+ E ++ YP  +VR + FD+ +QVQ ISF+  +P G
Sbjct:  897 FAAPE-----NSFIANDNTVRFSGTAAGYYDNRYWTMWKLPMFGCTDASQVLREISECRRAYPQCYVR-LAFDSVKQVQVISFVVQRPSG 980

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS3_ACEAT (Ribulose bisphosphate carboxylase small chain 3, chloroplastic OS=Acetabularia acetabulum GN=RBCS-3 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.375e-18
Identity = 34/61 (55.74%), Postives = 44/61 (72.13%), Query Frame = 3

Query:  114 YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 296
            Y D RYWTMWKLPM+GCTDA+QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:  109 YQDNRYWTMWKLPMFGCTDASQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 169

HSP 2 Score: 30.0314 bits (66), Expect = 1.375e-18
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = 2

Query:    2 EALLKEISYLLRSGWIPCLEF 64
            E + K++ Y+L + W PCLEF
Sbjct:   66 EQISKQVDYILINSWTPCLEF 86

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS3_ACECL (Ribulose bisphosphate carboxylase small chain 3, chloroplastic OS=Acetabularia cliftonii GN=RBCS-3 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.788e-18
Identity = 34/64 (53.12%), Postives = 44/64 (68.75%), Query Frame = 3

Query:  105 SPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 296
            S  Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:  107 SSTYQDNRYWTMWKLPMFGCTDGSQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 170

HSP 2 Score: 30.8018 bits (68), Expect = 1.788e-18
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = 2

Query:    2 EALLKEISYLLRSGWIPCLEF 64
            E + K++ Y+L + W PCLEF
Sbjct:   67 EQISKQVDYILANSWTPCLEF 87

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS1_ACECL (Ribulose bisphosphate carboxylase small chain 1 (Fragment) OS=Acetabularia cliftonii GN=RBCS-1 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.833e-18
Identity = 35/73 (47.95%), Postives = 46/73 (63.01%), Query Frame = 3

Query:   78 DGCTCEHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 296
            D C      +  Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:   41 DNCIRMGPVASTYQDNRYWTMWKLPMFGCTDGSQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 113

HSP 2 Score: 30.8018 bits (68), Expect = 1.833e-18
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = 2

Query:    2 EALLKEISYLLRSGWIPCLEF 64
            E + K++ Y+L + W PCLEF
Sbjct:   10 EQISKQVDYILANSWTPCLEF 30

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS5_ACECL (Ribulose bisphosphate carboxylase small chain 5, chloroplastic OS=Acetabularia cliftonii GN=RBCS-5 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.325e-18
Identity = 33/61 (54.10%), Postives = 43/61 (70.49%), Query Frame = 3

Query:  114 YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 296
            Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:  111 YQDNRYWTMWKLPMFGCTDGSQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 171

HSP 2 Score: 30.8018 bits (68), Expect = 2.325e-18
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = 2

Query:    2 EALLKEISYLLRSGWIPCLEF 64
            E + K++ Y+L + W PCLEF
Sbjct:   68 EQISKQVDYILANSWTPCLEF 88

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS4_ACECL (Ribulose bisphosphate carboxylase small chain 4, chloroplastic OS=Acetabularia cliftonii GN=RBCS-4 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.325e-18
Identity = 33/61 (54.10%), Postives = 43/61 (70.49%), Query Frame = 3

Query:  114 YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 296
            Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:  109 YQDNRYWTMWKLPMFGCTDGSQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 169

HSP 2 Score: 30.8018 bits (68), Expect = 2.325e-18
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = 2

Query:    2 EALLKEISYLLRSGWIPCLEF 64
            E + K++ Y+L + W PCLEF
Sbjct:   66 EQISKQVDYILANSWTPCLEF 86

[Close]

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Match: RBS1_ACEAT (Ribulose bisphosphate carboxylase small chain 1, chloroplastic OS=Acetabularia acetabulum GN=RBCS-1 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.325e-18
Identity = 33/61 (54.10%), Postives = 43/61 (70.49%), Query Frame = 3

Query:  114 YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 296
            Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:  109 YQDNRYWTMWKLPMFGCTDGSQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 169

HSP 2 Score: 30.8018 bits (68), Expect = 2.325e-18
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = 2

Query:    2 EALLKEISYLLRSGWIPCLEF 64
            E + K++ Y+L + W PCLEF
Sbjct:   66 EQISKQVDYILTNSWTPCLEF 86

The following BLAST results are available for this feature:

BLAST of BQ623442 vs. ExPASy Swiss-Prot

BLAST of BQ623442 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 112


Match Name	E-value	Identity	Description
RBS_CHLMO	8.750e-22	58.73	Ribulose bisphosphate carboxylase small chain, chl...	[more]
RBS1_CHLRE	4.508e-20	55.00	Ribulose bisphosphate carboxylase small chain 1, c...	[more]
RBS2_CHLRE	5.864e-20	55.00	Ribulose bisphosphate carboxylase small chain 2, c...	[more]
RBS_EUGGR	1.068e-19	48.89	Ribulose bisphosphate carboxylase small chains, ch...	[more]
RBS3_ACEAT	1.375e-18	55.74	Ribulose bisphosphate carboxylase small chain 3, c...	[more]
RBS3_ACECL	1.788e-18	53.13	Ribulose bisphosphate carboxylase small chain 3, c...	[more]
RBS1_ACECL	1.833e-18	47.95	Ribulose bisphosphate carboxylase small chain 1 (F...	[more]
RBS5_ACECL	2.325e-18	54.10	Ribulose bisphosphate carboxylase small chain 5, c...	[more]
RBS4_ACECL	2.325e-18	54.10	Ribulose bisphosphate carboxylase small chain 4, c...	[more]
RBS1_ACEAT	2.325e-18	54.10	Ribulose bisphosphate carboxylase small chain 1, c...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_00533 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00533 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623442 ID=BQ623442; Name=BQ623442; organism=Citrus sinensis; type=EST; length=449bp
TGAAGCTTTGCTCAAGGAAATCAGTTACCTTCTCCGCTCCGGCTGGATTC
CCTGCTTGGAATTCGAGTTGGAGAAAGGATGGGTGTACCTGTGAGCACCA
CAGCTCACCAGGATACTACGATGGACGCTACTGGACAATGTGGAAGCTGC
CCATGTATGGGTGCACAGATGCAACCCAGGTTTTGAAGGAGGTTGGGGAG
GTCCAGAAGGAATACCCACATTCATTCGTCCGAATTATCGGATTTGACAA
CAAGCGTCAAGTGCAGTGCATCAGTTTCCTTGCTGCCAAGCCACCAGGCG
TCTAGGTTCCCAATTAAATTTGTGTAACCACCCATGCAATATTGTTGATT
ATTATTATGTGTAACCCCCATAATGATCATTCGATCAATGGGGGGTTCGG
TTTGCGTTTAATTTGTACTTAGGCTGCTGTTGCTGCTGCTCAAACTTTT