BQ623525 | Citrus Genome Database

Overview


Name	BQ623525
Unique Name	BQ623525
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	695

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2449	contig	Csv1_Contig2449:480..1174.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

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BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA2_SOYBN (Glutamine synthetase cytosolic isozyme 2 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 5.104e-92
Identity = 122/157 (77.71%), Postives = 132/157 (84.08%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            R+ AAKIF H DV AEEPWYG+EQEYTLLQKDV+WPL                   A+KA+GRDIVDSHYKAC+YAGINISGINGEVMPGQWEFQVGP++GISA D+LWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAH NYSTKSMRNDGG+
Sbjct:  107 RNNAAKIFGHPDVAAEEPWYGLEQEYTLLQKDVQWPLGWPLGGFPGPQGPYYCGTGANKAFGRDIVDSHYKACIYAGINISGINGEVMPGQWEFQVGPSIGISAADELWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHTNYSTKSMRNDGGY 263

HSP 2 Score: 100.523 bits (249), Expect = 5.104e-92
Identity = 50/61 (81.97%), Postives = 53/61 (86.89%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEK 653
            +E IKKAI KL  RH EHIAAYGEGNERRLTG+HETAD+NTF  GVANRGASIRVG DTEK
Sbjct:  263 YEVIKKAIAKLEKRHKEHIAAYGEGNERRLTGRHETADMNTFVWGVANRGASIRVGRDTEK 323

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLN12_ORYSJ (Glutamine synthetase cytosolic isozyme 1-2 OS=Oryza sativa subsp. japonica GN=GLN1-2 PE=1 SV=1)

HSP 1 Score: 249.98 bits (637), Expect = 6.658e-92
Identity = 117/157 (74.52%), Postives = 129/157 (82.17%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RH+AAKIFSH DVVAE PWYGIEQEYTLLQKDV WPL                   A+KA+GRDIVD+HYKAC+YAGINISGINGEVMPGQWEFQVGP+VGI+A DQ+WVARYILER+TE+AGVVLS DPKPI GDWNGAGAH N+STKSMR  GG+
Sbjct:  107 RHSAAKIFSHPDVVAEVPWYGIEQEYTLLQKDVNWPLGWPVGGFPGPQGPYYCAAGAEKAFGRDIVDAHYKACIYAGINISGINGEVMPGQWEFQVGPSVGIAAADQVWVARYILERVTEVAGVVLSLDPKPIPGDWNGAGAHTNFSTKSMREPGGY 263

HSP 2 Score: 108.997 bits (271), Expect = 6.658e-92
Identity = 53/62 (85.48%), Postives = 56/62 (90.32%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEKE 656
            +E IKKAI+KL LRH EHIAAYGEGNERRLTG+HETADINTF  GVANRGASIRVG DTEKE
Sbjct:  263 YEVIKKAIDKLALRHKEHIAAYGEGNERRLTGRHETADINTFKWGVANRGASIRVGRDTEKE 324

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLN14_ARATH (Glutamine synthetase cytosolic isozyme 1-4 OS=Arabidopsis thaliana GN=GLN1-4 PE=1 SV=1)

HSP 1 Score: 248.825 bits (634), Expect = 1.478e-91
Identity = 118/157 (75.16%), Postives = 126/157 (80.25%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RHAAAKIF    VVAEE WYGIEQEYTLLQKD+KWP+                   ADKA+GRDIVDSHYKACLYAGIN+SG NGEVMPGQWEFQVGP VGI+A DQ+WVARYILERITE+AGVVLS DPKPI GDWNGAGAH NYSTKSMR DGG+
Sbjct:  107 RHAAAKIFEDPSVVAEETWYGIEQEYTLLQKDIKWPVGWPVGGFPGPQGPYYCGVGADKAFGRDIVDSHYKACLYAGINVSGTNGEVMPGQWEFQVGPTVGIAAADQVWVARYILERITELAGVVLSLDPKPIPGDWNGAGAHTNYSTKSMREDGGY 263

HSP 2 Score: 108.997 bits (271), Expect = 1.478e-91
Identity = 54/61 (88.52%), Postives = 56/61 (91.80%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEK 653
            +E IKKAIEKLGLRH EHIAAYGEGNERRLTGKHETADINTF  GVANRGASIRVG DTE+
Sbjct:  263 YEVIKKAIEKLGLRHKEHIAAYGEGNERRLTGKHETADINTFLWGVANRGASIRVGRDTEQ 323

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLN12_ARATH (Glutamine synthetase cytosolic isozyme 1-2 OS=Arabidopsis thaliana GN=GLN1-2 PE=1 SV=2)

HSP 1 Score: 247.284 bits (630), Expect = 5.585e-91
Identity = 116/157 (73.89%), Postives = 130/157 (82.80%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RHAAA+IF++ DV+AE PWYGIEQEYTLLQKDV WPL                   ADK++GRDIVD+HYKA LYAGINISGINGEVMPGQWEFQVGP+VGISA D++W+ARYILERITEIAGVV+SFDPKPI GDWNGAGAH NYSTKSMR +GG+
Sbjct:  107 RHAAAEIFANPDVIAEVPWYGIEQEYTLLQKDVNWPLGWPIGGFPGPQGPYYCSIGADKSFGRDIVDAHYKASLYAGINISGINGEVMPGQWEFQVGPSVGISAADEIWIARYILERITEIAGVVVSFDPKPIPGDWNGAGAHTNYSTKSMREEGGY 263

HSP 2 Score: 108.612 bits (270), Expect = 5.585e-91
Identity = 54/62 (87.10%), Postives = 56/62 (90.32%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEKE 656
            +E IKKAIEKLGLRH EHI+AYGEGNERRLTG HETADINTF  GVANRGASIRVG DTEKE
Sbjct:  263 YEIIKKAIEKLGLRHKEHISAYGEGNERRLTGHHETADINTFLWGVANRGASIRVGRDTEKE 324

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA2_MAIZE (Glutamine synthetase root isozyme 2 OS=Zea mays GN=GLN2 PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 1.356e-89
Identity = 121/157 (77.07%), Postives = 129/157 (82.17%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RH AAKIFSH DV AEEPW+GIEQEYTLLQKD KWPL                   ADK++GRDIVD  YKACLYAGI+ISGINGEVMPGQWEFQV PAVG+SAGDQLWVARYILERITEIAGVV+SFDPKPI GDWNGAGAH NYSTKSMR+DGG+
Sbjct:  107 RHGAAKIFSHPDVKAEEPWFGIEQEYTLLQKDTKWPLGWPLAYPGPQGPYYCAAG-ADKSYGRDIVDCAYKACLYAGIDISGINGEVMPGQWEFQVAPAVGVSAGDQLWVARYILERITEIAGVVVSFDPKPIPGDWNGAGAHTNYSTKSMRSDGGY 262

HSP 2 Score: 102.064 bits (253), Expect = 1.356e-89
Identity = 50/62 (80.65%), Postives = 54/62 (87.10%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEKE 656
            +E IKKAI KLGLRH EHIAAYG+GNER LTG+HETADINTF  GV NRGAS+RVG DTEKE
Sbjct:  262 YEVIKKAIGKLGLRHREHIAAYGDGNERPLTGRHETADINTFVWGVPNRGASVRVGRDTEKE 323

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLN13_ORYSJ (Glutamine synthetase cytosolic isozyme 1-3 OS=Oryza sativa subsp. japonica GN=GLN1-3 PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 5.122e-89
Identity = 114/157 (72.61%), Postives = 128/157 (81.53%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            R+ AA+IFSH DV AEEPWYGIEQEYTLLQK + WPL                   ADK++GRDIVD+HYKACL+AGINISGIN EVMPGQWEFQ+GP VG+SAGD +WVARYILERITEIAGVV+SFDPKPI GDWNGAGAH NYSTKSMR++GG+
Sbjct:  109 RYNAARIFSHPDVKAEEPWYGIEQEYTLLQKHINWPLGWPLGGYPGPQGPYYCAAGADKSYGRDIVDAHYKACLFAGINISGINAEVMPGQWEFQIGPVVGVSAGDHVWVARYILERITEIAGVVVSFDPKPIPGDWNGAGAHTNYSTKSMRSNGGY 265

HSP 2 Score: 103.99 bits (258), Expect = 5.122e-89
Identity = 49/62 (79.03%), Postives = 56/62 (90.32%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEKE 656
            +E IKKAI+KLG+RH EHIAAYG+GNERRLTG+HETADIN F  GVANRGAS+RVG DTEK+
Sbjct:  265 YEVIKKAIKKLGMRHREHIAAYGDGNERRLTGRHETADINNFVWGVANRGASVRVGRDTEKD 326

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA_LACSA (Glutamine synthetase OS=Lactuca sativa PE=2 SV=2)

HSP 1 Score: 237.654 bits (605), Expect = 6.683e-89
Identity = 113/157 (71.97%), Postives = 125/157 (79.62%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            R AAAKIFS+ +V  E  WYGIEQEYTLLQKD  WPL                   ADKA+GRDIVD+HYKACLYAG+NISGINGEVMPGQWEFQVGP+VGI+A DQ+WVARYILERITEI GVV+SFDPKPI GDWNGAGAH NYSTK+MR +GG+
Sbjct:  107 RAAAAKIFSNPEVEKEVTWYGIEQEYTLLQKDTNWPLGWPLGGFPGPQGPYYCGIGADKAFGRDIVDAHYKACLYAGVNISGINGEVMPGQWEFQVGPSVGIAAADQIWVARYILERITEIYGVVVSFDPKPIPGDWNGAGAHTNYSTKTMREEGGY 263

HSP 2 Score: 111.309 bits (277), Expect = 6.683e-89
Identity = 55/62 (88.71%), Postives = 57/62 (91.94%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEKE 656
            +E IKKAIEKLGLRH EHIAAYGEGNERRLTG+HETADINTF  GVANRGASIRVG DTEKE
Sbjct:  263 YEVIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHETADINTFLWGVANRGASIRVGRDTEKE 324

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA3_LUPAN (Glutamine synthetase nodule isozyme (Fragment) OS=Lupinus angustifolius PE=2 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 8.726e-89
Identity = 122/157 (77.71%), Postives = 131/157 (83.44%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RHAAAKIFSH DV AEEPW+GIEQEYTLLQKD+ WP+                   A+KA+GRDIVDSHYKACLYAGINISGIN EVMPGQWEFQVGP++GISAGD+LWVARYILERITEIAGVVLS DPKPI GDWNGAGAH NYSTKSMRNDGG+
Sbjct:  107 RHAAAKIFSHPDVAAEEPWFGIEQEYTLLQKDIHWPIGMALGGFPGPQGPYYCGTGAEKAFGRDIVDSHYKACLYAGINISGINAEVMPGQWEFQVGPSIGISAGDELWVARYILERITEIAGVVLSLDPKPIPGDWNGAGAHTNYSTKSMRNDGGY 263

HSP 2 Score: 89.3521 bits (220), Expect = 8.726e-89
Identity = 43/53 (81.13%), Postives = 49/53 (92.45%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASI 629
            +E IK+AIEKL  RH+EHIAAYGEGNERRLTG+HETADI+TF+ GVANRGASI
Sbjct:  263 YEVIKQAIEKLEKRHNEHIAAYGEGNERRLTGRHETADISTFSWGVANRGASI 315

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA3_HORVU (Glutamine synthetase OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 1.244e-87
Identity = 117/157 (74.52%), Postives = 128/157 (81.53%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            R+ AAKI S+ DV  EEPWYGIEQEYTLLQKD+ WPL                   ADK++GRDIVDSHYKACL+ G+NISGINGEVMPGQWEFQVGP VGISAGDQ+WVARYILERITEIAGVV++FDPKPI GDWNGAGAH NYST+SMRNDGGF
Sbjct:  107 RYNAAKILSNPDVAKEEPWYGIEQEYTLLQKDINWPLGWPVGGFPGPQGPYYCGIGADKSFGRDIVDSHYKACLFGGVNISGINGEVMPGQWEFQVGPTVGISAGDQVWVARYILERITEIAGVVVTFDPKPIPGDWNGAGAHTNYSTESMRNDGGF 263

HSP 2 Score: 95.5153 bits (236), Expect = 1.244e-87
Identity = 46/61 (75.41%), Postives = 53/61 (86.89%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEK 653
            F+ I  A+EKL L+H EHIAAYGEGNERRLTGKHETADINT + GVANRGAS+RVG +TE+
Sbjct:  263 FKVIVDAVEKLKLKHKEHIAAYGEGNERRLTGKHETADINTSSWGVANRGASVRVGRETEQ 323

[Close]

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Match: GLNA2_MEDSA (Glutamine synthetase leaf isozyme, chloroplastic OS=Medicago sativa GN=GS2 PE=2 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 8.002e-84
Identity = 111/157 (70.70%), Postives = 122/157 (77.71%), Query Frame = 2

Query:    2 RHAAAKIFSHSDVVAEEPWYGIEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDIVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAGDQLWVARYILERITEIAGVVLSFDPKPIQGDWNGAGAHANYSTKSMRNDGGF 472
            RH AA+IFS+  V AE PWYGIEQEYTLLQ DVKWPL                   ADK++GRDI D+HYKACLYAGINISG NGEVMPGQWE+QVGP+VGI AGD +W +RYILERITE AGVVL+ DPKPI+GDWNGAG H NYSTKSMR DGGF
Sbjct:  163 RHKAAEIFSNPKVEAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQGPYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWASRYILERITEQAGVVLTLDPKPIEGDWNGAGCHTNYSTKSMREDGGF 319

HSP 2 Score: 97.4413 bits (241), Expect = 8.002e-84
Identity = 49/61 (80.33%), Postives = 50/61 (81.97%), Query Frame = 3

Query:  471 FEGIKKAIEKLGLRHSEHIAAYGEGNERRLTGKHETADINTFNGGVANRGASIRVGPDTEK 653
            FE IKKAI  L LRH  HI AYGEGNERRLTGKHETA INTF+ GVANRG SIRVG DTEK
Sbjct:  319 FEVIKKAILNLSLRHKVHIEAYGEGNERRLTGKHETASINTFSWGVANRGCSIRVGRDTEK 379

The following BLAST results are available for this feature:

BLAST of BQ623525 vs. ExPASy Swiss-Prot

BLAST of BQ623525 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 88


Match Name	E-value	Identity	Description
GLNA2_SOYBN	5.104e-92	77.71	Glutamine synthetase cytosolic isozyme 2 OS=Glycin...	[more]
GLN12_ORYSJ	6.658e-92	74.52	Glutamine synthetase cytosolic isozyme 1-2 OS=Oryz...	[more]
GLN14_ARATH	1.478e-91	75.16	Glutamine synthetase cytosolic isozyme 1-4 OS=Arab...	[more]
GLN12_ARATH	5.585e-91	73.89	Glutamine synthetase cytosolic isozyme 1-2 OS=Arab...	[more]
GLNA2_MAIZE	1.356e-89	77.07	Glutamine synthetase root isozyme 2 OS=Zea mays GN...	[more]
GLN13_ORYSJ	5.122e-89	72.61	Glutamine synthetase cytosolic isozyme 1-3 OS=Oryz...	[more]
GLNA_LACSA	6.683e-89	71.97	Glutamine synthetase OS=Lactuca sativa PE=2 SV=2	[more]
GLNA3_LUPAN	8.726e-89	77.71	Glutamine synthetase nodule isozyme (Fragment) OS=...	[more]
GLNA3_HORVU	1.244e-87	74.52	Glutamine synthetase OS=Hordeum vulgare PE=2 SV=1	[more]
GLNA2_MEDSA	8.002e-84	70.70	Glutamine synthetase leaf isozyme, chloroplastic O...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_00616 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00616 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623525 ID=BQ623525; Name=BQ623525; organism=Citrus sinensis; type=EST; length=695bp
AAGACATGCAGCTGCCAAGATTTTCAGCCATTCTGACGTTGTGGCTGAAG
AACCATGGTATGGTATTGAGCAGGAATACACCTTACTGCAGAAGGATGTT
AAATGGCCTCTTGGCTGGCCCATCGGTGGTTATCCTGGGCCTCAGGGACC
ATACTACTGTGGTGTTGGTGCTGACAAAGCTTGGGGGCGCGACATTGTAG
ACTCCCATTACAAGGCCTGTCTATATGCAGGAATCAACATCAGTGGCATC
AATGGAGAAGTCATGCCGGGGCAGTGGGAGTTCCAAGTTGGTCCCGCTGT
TGGAATTTCTGCTGGAGATCAGTTGTGGGTTGCCCGCTATATTTTAGAGA
GGATTACAGAGATTGCTGGAGTTGTGCTTTCTTTCGATCCCAAGCCAATT
CAGGGTGACTGGAATGGAGCTGGTGCTCACGCAAATTACAGCACCAAGTC
GATGAGGAACGATGGAGGCTTTTGAGGGTATTAAGAAAGCAATTGAGAAA
CTTGGACTGAGGCACAGTGAGCACATTGCCGCATATGGTGAAGGCAATGA
ACGCCGACTCACTGGCAAACATGAAACAGCTGACATCAACACTTTCAATG
GGGGTGTTGCTAACCGTGGGGCATCAATCCGTGTTGGACCAGACACAGAG
AAAGAAGGAAGGGGTACTTTGAGGACAGGAGACCTGCTTTTAACA