BQ623627

Overview
NameBQ623627
Unique NameBQ623627
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length715
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_MYCLE (Malate dehydrogenase OS=Mycobacterium leprae GN=mdh PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.750e-25
Identity = 51/91 (56.04%), Postives = 62/91 (68.13%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII AR             D  RDW+LGTPEG WVSM V SDGSY VP GLI SFPVT  +G+WTIV+GL ID+FSR ++D +  EL++E+
Sbjct:  230 AIIDARGASSAASAASATIDAARDWLLGTPEGNWVSMAVVSDGSYGVPEGLISSFPVTTTDGDWTIVRGLGIDDFSRGRIDKSTAELADER 320

HSP 2 Score: 30.4166 bits (67), Expect = 4.750e-25
Identity = 14/28 (50.00%), Postives = 17/28 (60.71%), Query Frame = 2 
Query:   29 GEKPVRELVKDDAWLNGEFITTVQQRGA 112
            G K   E+V D AW+   FI TV +RGA
Sbjct:  202 GGKNAAEVVGDQAWIEDYFIPTVAKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_AKKM8 (Malate dehydrogenase OS=Akkermansia muciniphila (strain ATCC BAA-835) GN=mdh PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.047e-24
Identity = 46/92 (50.00%), Postives = 55/92 (59.78%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPV-TCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AIIKAR             D +R     TPEG W S+ V SDGSY +  GLI SFPV T ++G W IVQGL +D FSR+K+D T  EL EE+
Sbjct:  229 AIIKARGASSAASAASAAVDTVRSLATQTPEGDWYSVAVCSDGSYGIEKGLICSFPVRTTKDGGWEIVQGLPVDAFSREKIDATVNELKEER 320

HSP 2 Score: 48.1358 bits (113), Expect = 1.047e-24
Identity = 21/32 (65.62%), Postives = 24/32 (75.00%), Query Frame = 2 
Query:   17 NTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            N   G KPV E++KD  WL G+FITTVQQRGA
Sbjct:  197 NARIGGKPVTEVIKDTEWLKGDFITTVQQRGA 228
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_THEFY (Malate dehydrogenase OS=Thermobifida fusca (strain YX) GN=mdh PE=3 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 1.773e-24
Identity = 49/91 (53.85%), Postives = 60/91 (65.93%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+ DWV GTPEG W S+ + SDGSY VP GL+ SFPV  RNG W IVQGL I+EFSR+++D +  EL EE+
Sbjct:  230 AIIEARGASSAASAANAAIDHVYDWVNGTPEGDWTSVALPSDGSYGVPEGLVSSFPVVSRNGSWEIVQGLEINEFSRERIDASVRELEEER 320

HSP 2 Score: 29.6462 bits (65), Expect = 1.773e-24
Identity = 18/36 (50.00%), Postives = 20/36 (55.56%), Query Frame = 2 
Query:    5 HATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            HA VN   G   V E V D+ WL   FI TV +RGA
Sbjct:  198 HAEVN---GRSAV-EAVNDEEWLRDTFIPTVAKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_MYCTU (Malate dehydrogenase OS=Mycobacterium tuberculosis GN=mdh PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.308e-24
Identity = 50/92 (54.35%), Postives = 59/92 (64.13%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEKA 389
            AII AR             D  RDW+LGTP   WVSM V SDGSY VP GLI SFPVT + G WTIV GL IDEFSR ++D +  EL++E++
Sbjct:  230 AIIDARGASSAASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLEIDEFSRGRIDKSTAELADERS 321

HSP 2 Score: 32.3426 bits (72), Expect = 2.308e-24
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:   35 KPVRELVKDDAWLNGEFITTVQQRGA 112
            K   E+V D AW+  EFI TV +RGA
Sbjct:  204 KNAAEVVNDQAWIEDEFIPTVAKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_MYCTA (Malate dehydrogenase OS=Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) GN=mdh PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.308e-24
Identity = 50/92 (54.35%), Postives = 59/92 (64.13%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEKA 389
            AII AR             D  RDW+LGTP   WVSM V SDGSY VP GLI SFPVT + G WTIV GL IDEFSR ++D +  EL++E++
Sbjct:  230 AIIDARGASSAASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLEIDEFSRGRIDKSTAELADERS 321

HSP 2 Score: 32.3426 bits (72), Expect = 2.308e-24
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:   35 KPVRELVKDDAWLNGEFITTVQQRGA 112
            K   E+V D AW+  EFI TV +RGA
Sbjct:  204 KNAAEVVNDQAWIEDEFIPTVAKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_MYCBT (Malate dehydrogenase OS=Mycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019) GN=mdh PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.308e-24
Identity = 50/92 (54.35%), Postives = 59/92 (64.13%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEKA 389
            AII AR             D  RDW+LGTP   WVSM V SDGSY VP GLI SFPVT + G WTIV GL IDEFSR ++D +  EL++E++
Sbjct:  230 AIIDARGASSAASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLEIDEFSRGRIDKSTAELADERS 321

HSP 2 Score: 32.3426 bits (72), Expect = 2.308e-24
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:   35 KPVRELVKDDAWLNGEFITTVQQRGA 112
            K   E+V D AW+  EFI TV +RGA
Sbjct:  204 KNAAEVVNDQAWIEDEFIPTVAKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_MYCBP (Malate dehydrogenase OS=Mycobacterium bovis (strain BCG / Pasteur 1173P2) GN=mdh PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.308e-24
Identity = 50/92 (54.35%), Postives = 59/92 (64.13%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEKA 389
            AII AR             D  RDW+LGTP   WVSM V SDGSY VP GLI SFPVT + G WTIV GL IDEFSR ++D +  EL++E++
Sbjct:  230 AIIDARGASSAASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLEIDEFSRGRIDKSTAELADERS 321

HSP 2 Score: 32.3426 bits (72), Expect = 2.308e-24
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:   35 KPVRELVKDDAWLNGEFITTVQQRGA 112
            K   E+V D AW+  EFI TV +RGA
Sbjct:  204 KNAAEVVNDQAWIEDEFIPTVAKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_MYCBO (Malate dehydrogenase OS=Mycobacterium bovis GN=mdh PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.308e-24
Identity = 50/92 (54.35%), Postives = 59/92 (64.13%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEKA 389
            AII AR             D  RDW+LGTP   WVSM V SDGSY VP GLI SFPVT + G WTIV GL IDEFSR ++D +  EL++E++
Sbjct:  230 AIIDARGASSAASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLEIDEFSRGRIDKSTAELADERS 321

HSP 2 Score: 32.3426 bits (72), Expect = 2.308e-24
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:   35 KPVRELVKDDAWLNGEFITTVQQRGA 112
            K   E+V D AW+  EFI TV +RGA
Sbjct:  204 KNAAEVVNDQAWIEDEFIPTVAKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_PSYCK (Malate dehydrogenase OS=Psychrobacter cryohalolentis (strain K5) GN=mdh PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.004e-24
Identity = 48/92 (52.17%), Postives = 62/92 (67.39%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSI-DEFSRKKLDLTAEELSEEK 386
            AIIKAR              H+R WV+GT E  WVSMGVYS+G Y +  GLIYSFPVTC NG+W+IV G+ +  +FS++K+  T +ELSEE+
Sbjct:  230 AIIKARGASSAASAANAAIAHVRTWVMGTDENDWVSMGVYSNGEYGIAKGLIYSFPVTCANGDWSIVDGVDVSSDFSKEKMAATEQELSEER 321

HSP 2 Score: 30.8018 bits (68), Expect = 3.004e-24
Identity = 18/34 (52.94%), Postives = 21/34 (61.76%), Query Frame = 2 
Query:   11 TVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            T +T  G KP  +LV D AW  G +I  VQQRGA
Sbjct:  198 TASTVNG-KPALDLV-DRAWYEGTYIPEVQQRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_STRCO (Malate dehydrogenase OS=Streptomyces coelicolor GN=mdh PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 8.614e-24
Identity = 48/91 (52.75%), Postives = 61/91 (67.03%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+  WV GT EG W SMG+ SDGSY VP G+I SFPVT ++G + IVQGL I+EFSR ++D + +ELSEE+
Sbjct:  230 AIIEARGASSAASAANAAIDHVYTWVNGTAEGDWTSMGIPSDGSYGVPEGIISSFPVTTKDGSYEIVQGLDINEFSRARIDASVKELSEER 320

HSP 2 Score: 32.3426 bits (72), Expect = 8.614e-24
Identity = 20/36 (55.56%), Postives = 22/36 (61.11%), Query Frame = 2 
Query:    5 HATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            HATV   AG K   E V D+ WL  EFI TV +RGA
Sbjct:  198 HATV---AG-KNAAETVNDEKWLADEFIPTVAKRGA 229
The following BLAST results are available for this feature:
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 166
Match NameE-valueIdentityDescription
MDH_MYCLE4.750e-2556.04Malate dehydrogenase OS=Mycobacterium leprae GN=md... [more]
MDH_AKKM81.047e-2450.00Malate dehydrogenase OS=Akkermansia muciniphila (s... [more]
MDH_THEFY1.773e-2453.85Malate dehydrogenase OS=Thermobifida fusca (strain... [more]
MDH_MYCTU2.308e-2454.35Malate dehydrogenase OS=Mycobacterium tuberculosis... [more]
MDH_MYCTA2.308e-2454.35Malate dehydrogenase OS=Mycobacterium tuberculosis... [more]
MDH_MYCBT2.308e-2454.35Malate dehydrogenase OS=Mycobacterium bovis (strai... [more]
MDH_MYCBP2.308e-2454.35Malate dehydrogenase OS=Mycobacterium bovis (strai... [more]
MDH_MYCBO2.308e-2454.35Malate dehydrogenase OS=Mycobacterium bovis GN=mdh... [more]
MDH_PSYCK3.004e-2452.17Malate dehydrogenase OS=Psychrobacter cryohalolent... [more]
MDH_STRCO8.614e-2452.75Malate dehydrogenase OS=Streptomyces coelicolor GN... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00718 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00718 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623627 ID=BQ623627; Name=BQ623627; organism=Citrus sinensis; type=EST; length=715bp
CAACCATGCAACTGTCAATACTGCAGCTGGGGAAAAGCCTGTCCGTGAGC
TTGTCAAGGATGATGCATGGTTGAATGGAGAATTCATAACCACAGTCCAA
CAACGTGGTGCTGGCAATCATCAAAGCCAGAAAGCTCTCGAGTGCTCTGT
CTGCTGCTAGCTCTGCTTGTGACCATATCCGTGATTGGGTTCTTGGAACT
CCTGAGGGCACCTGGGTCTCCATGGGGGTATACTCTGATGGTTCATACAA
TGTACCAGCTGGGCTCATTTACTCATTCCCAGTAACCTGCCGCAACGGTG
AATGGACAATTGTTCAAGGACTCAGCATTGATGAATTCTCAAGGAAGAAG
TTGGACTTGACAGCAGAGGAGCTCTCCGAGGAGAAGGCCCTCGCATACTC
ATGTCTCTCTTAAATGCTTTCTTCCCTTCAATAGTGTGTTACCAAGGCCC
TTAGCTTTGTCTGAGTAGTTGGTATAGTTACAGTTTCGAATAAAACCATT
GTTGACACCTGGAGGAACCATTGATGATGGAAAAGCCTTTCCATCTTATG
TTGCTATTTCTGCATTTGAATATCGGGATTTGGGAGTTTTGGTACTTTTC
CTGGATAAATGGATATAAAGGCCCATTGGGATTTATGTATCAAACTATCA
ATAACAATTTTTTTTTTTGTTTTAACAAAAAAAGAAAAAAAATAAAAAAT
TTAAATAAACCTCAG
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