BQ623627 | Citrus Genome Database

Overview


Name	BQ623627
Unique Name	BQ623627
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	715

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BDEBA (Malate dehydrogenase OS=Bdellovibrio bacteriovorus GN=mdh PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.121e-23
Identity = 51/91 (56.04%), Postives = 66/91 (72.53%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DHIRDW LGT  G WV+MG+ SDGSY++P G++Y FPVTC+NGE+ IV+GL ID FSR+K++ T +EL+EEK
Sbjct:  239 AIIEARGLSSAASAASAAVDHIRDWWLGT-NGEWVTMGIPSDGSYDIPEGIMYGFPVTCKNGEYEIVKGLEIDAFSREKMNNTLKELNEEK 328

HSP 2 Score: 25.0238 bits (53), Expect = 1.121e-23
Identity = 18/36 (50.00%), Postives = 20/36 (55.56%), Query Frame = 2

Query:   20 TAAGEKPVRELVK-----DDAWLNGEFITTVQQRGA 112
            TA G K V EL+K      DAW    FI TV +RGA
Sbjct:  204 TADGAK-VPELLKLGTAEGDAWNKDTFIPTVGKRGA 238

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_AZOSB (Malate dehydrogenase OS=Azoarcus sp. (strain BH72) GN=mdh PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.459e-23
Identity = 51/91 (56.04%), Postives = 63/91 (69.23%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DHIRDWVLG+  G WV+MG+ SDGSY +P G+IY FPVT  NGE+ IVQGL IDEFSR+++ +T  EL EE+
Sbjct:  233 AIIEARGLSSAASAANAAIDHIRDWVLGS-NGEWVTMGIPSDGSYGIPEGVIYGFPVTTENGEYKIVQGLEIDEFSRERMTVTLNELLEER 322

HSP 2 Score: 26.9498 bits (58), Expect = 1.459e-23
Identity = 11/24 (45.83%), Postives = 16/24 (66.67%), Query Frame = 2

Query:   41 VRELVKDDAWLNGEFITTVQQRGA 112
            V++L+ D AW    F+ TV +RGA
Sbjct:  209 VKDLINDAAWNKDVFLPTVGKRGA 232

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_LARHH (Malate dehydrogenase OS=Laribacter hongkongensis (strain HLHK9) GN=mdh PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.898e-23
Identity = 50/91 (54.95%), Postives = 62/91 (68.13%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DHIRDWVLGT  G WV+MG+ SDGSY +P G+ Y FPVTC++G++ IVQGL IDEFSR  ++ T  EL EE+
Sbjct:  232 AIIEARGLSSAASAANAAIDHIRDWVLGT-NGKWVTMGIPSDGSYGIPEGVTYGFPVTCKDGKYEIVQGLEIDEFSRACMNKTLAELEEER 321

HSP 2 Score: 28.4906 bits (62), Expect = 1.898e-23
Identity = 16/35 (45.71%), Postives = 22/35 (62.86%), Query Frame = 2

Query:    8 ATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            ATVN   GE  V+ ++ D+AW    F+ TV +RGA
Sbjct:  201 ATVN---GES-VKSMINDEAWNRDVFLPTVGKRGA 231

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BORA1 (Malate dehydrogenase OS=Bordetella avium (strain 197N) GN=mdh PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.898e-23
Identity = 47/91 (51.65%), Postives = 63/91 (69.23%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLG+  G WV+MG+ SDGSY +P G+IY FPVT  NGE+T+V+ L +D FSR+++D T +EL EE+
Sbjct:  232 AIIEARGLSSAASAANAAIDHVRDWVLGS-NGKWVTMGIPSDGSYGIPEGIIYGFPVTTANGEYTMVKDLEVDAFSRERMDFTLKELLEER 321

HSP 2 Score: 30.0314 bits (66), Expect = 1.898e-23
Identity = 12/26 (46.15%), Postives = 18/26 (69.23%), Query Frame = 2

Query:   35 KPVRELVKDDAWLNGEFITTVQQRGA 112
            +P+ +L+ D+AW    FI TV +RGA
Sbjct:  206 QPLAKLINDEAWNRDTFIPTVGKRGA 231

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_NITEU (Malate dehydrogenase OS=Nitrosomonas europaea GN=mdh PE=3 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.898e-23
Identity = 44/98 (44.90%), Postives = 60/98 (61.22%), Query Frame = 3

Query:   90 PQSNNVVLAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEE 383
            P   N    +I+AR             DH+RDW+ GT +  W++MG+ SDGSY +P G+IY FPV C+NG   IVQGL I  FSR +LD+  +EL++E
Sbjct:  221 PTVQNRGAVVIEARGLSSAASAANAIIDHMRDWIFGTRDDDWITMGILSDGSYKIPKGVIYGFPVVCKNGGRKIVQGLEISPFSRTRLDIAYDELTQE 318

HSP 2 Score: 32.3426 bits (72), Expect = 1.898e-23
Identity = 15/37 (40.54%), Postives = 22/37 (59.46%), Query Frame = 2

Query:    2 NHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            +HA ++     + V +L+KD  W+   FI TVQ RGA
Sbjct:  196 SHAEID----HQKVIDLIKDQTWVENSFIPTVQNRGA 228

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_CHRVO (Malate dehydrogenase OS=Chromobacterium violaceum GN=mdh PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 2.470e-23
Identity = 51/92 (55.43%), Postives = 62/92 (67.39%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEKA 389
            AII+AR             DHIRDWVLGT  G WV+MGV SDGSY +P G++Y  PV C NGE+  V+GL ID FSR+++DLT  EL EE+A
Sbjct:  229 AIIEARGLSSAASAANAAIDHIRDWVLGT-NGKWVTMGVPSDGSYGIPEGVMYGVPVVCENGEYKRVEGLEIDAFSRERMDLTLAELEEERA 319

HSP 2 Score: 26.5646 bits (57), Expect = 2.470e-23
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 2

Query:   41 VRELVKDDAWLNGEFITTVQQRGA 112
            V+ ++ DD W    F+ TV +RGA
Sbjct:  205 VKAMINDDVWNRDVFLPTVGKRGA 228

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_SACEN (Malate dehydrogenase OS=Saccharopolyspora erythraea (strain NRRL 23338) GN=mdh PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.470e-23
Identity = 51/91 (56.04%), Postives = 59/91 (64.84%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DHI  WV GTPEG W S  V SDGSY VP GLI SFPV  R+G + IVQGL IDEFSR+++D +  ELSEE+
Sbjct:  229 AIIEARGASSAASAANAAIDHIHTWVNGTPEGDWTSAAVVSDGSYGVPEGLISSFPVVARDGRYEIVQGLEIDEFSRQRIDASVNELSEER 319

HSP 2 Score: 27.335 bits (59), Expect = 2.470e-23
Identity = 16/36 (44.44%), Postives = 18/36 (50.00%), Query Frame = 2

Query:    5 HATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            HA VN     + V     D AWL   FI TV +RGA
Sbjct:  198 HAEVNGKVAAEQV-----DQAWLADTFIPTVAKRGA 228

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH1_BURTA (Malate dehydrogenase 1 OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=mdh1 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.470e-23
Identity = 49/91 (53.85%), Postives = 60/91 (65.93%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLGT  G WV+MG+ SDGSY +P  +IY  PVTC NGE+  V+GL ID FSR+K+D T  EL EE+
Sbjct:  230 AIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLAELLEER 319

HSP 2 Score: 30.8018 bits (68), Expect = 2.470e-23
Identity = 16/31 (51.61%), Postives = 20/31 (64.52%), Query Frame = 2

Query:   20 TAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            TA GE  ++ L+ DDAW    FI TV +RGA
Sbjct:  200 TAEGESLLK-LINDDAWNRDTFIPTVGKRGA 229

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BURM1 (Malate dehydrogenase OS=Burkholderia multivorans (strain ATCC 17616 / 249) GN=mdh PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 3.214e-23
Identity = 49/91 (53.85%), Postives = 60/91 (65.93%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLGT  G WV+MG+ SDGSY +P  +IY  PVTC NGE+  V+GL ID FSR+K+D T  EL EE+
Sbjct:  230 AIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDATLAELLEER 319

HSP 2 Score: 29.261 bits (64), Expect = 3.214e-23
Identity = 15/31 (48.39%), Postives = 19/31 (61.29%), Query Frame = 2

Query:   20 TAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            TA GE  ++ L+ DD W    FI TV +RGA
Sbjct:  200 TAEGESLLK-LINDDEWNRNTFIPTVGKRGA 229

[Close]

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_ACIBY (Malate dehydrogenase OS=Acinetobacter baumannii (strain AYE) GN=mdh PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 4.183e-23
Identity = 50/92 (54.35%), Postives = 63/92 (68.48%), Query Frame = 3

Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEKA 389
            AII+AR             DH+RDW LGT  G WV+MGV SDGSY +P G+++ FPVT  NGE+ IVQGL IDEFSR++++ T  EL EE+A
Sbjct:  231 AIIEARGLSSAASAANAAIDHMRDWALGT-NGKWVTMGVPSDGSYGIPEGVMFGFPVTTENGEYKIVQGLEIDEFSRERINFTLNELEEERA 321

HSP 2 Score: 26.1794 bits (56), Expect = 4.183e-23
Identity = 13/31 (41.94%), Postives = 19/31 (61.29%), Query Frame = 2

Query:   20 TAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            TA GE  +++ + D AW    F+ TV +RGA
Sbjct:  201 TANGES-LKDKINDPAWNKDVFLPTVGKRGA 230

The following BLAST results are available for this feature:

BLAST of BQ623627 vs. ExPASy Swiss-Prot

BLAST of BQ623627 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 166


Match Name	E-value	Identity	Description
MDH_BDEBA	1.121e-23	56.04	Malate dehydrogenase OS=Bdellovibrio bacteriovorus...	[more]
MDH_AZOSB	1.459e-23	56.04	Malate dehydrogenase OS=Azoarcus sp. (strain BH72)...	[more]
MDH_LARHH	1.898e-23	54.95	Malate dehydrogenase OS=Laribacter hongkongensis (...	[more]
MDH_BORA1	1.898e-23	51.65	Malate dehydrogenase OS=Bordetella avium (strain 1...	[more]
MDH_NITEU	1.898e-23	44.90	Malate dehydrogenase OS=Nitrosomonas europaea GN=m...	[more]
MDH_CHRVO	2.470e-23	55.43	Malate dehydrogenase OS=Chromobacterium violaceum ...	[more]
MDH_SACEN	2.470e-23	56.04	Malate dehydrogenase OS=Saccharopolyspora erythrae...	[more]
MDH1_BURTA	2.470e-23	53.85	Malate dehydrogenase 1 OS=Burkholderia thailandens...	[more]
MDH_BURM1	3.214e-23	53.85	Malate dehydrogenase OS=Burkholderia multivorans (...	[more]
MDH_ACIBY	4.183e-23	54.35	Malate dehydrogenase OS=Acinetobacter baumannii (s...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_00718 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00718 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623627 ID=BQ623627; Name=BQ623627; organism=Citrus sinensis; type=EST; length=715bp
CAACCATGCAACTGTCAATACTGCAGCTGGGGAAAAGCCTGTCCGTGAGC
TTGTCAAGGATGATGCATGGTTGAATGGAGAATTCATAACCACAGTCCAA
CAACGTGGTGCTGGCAATCATCAAAGCCAGAAAGCTCTCGAGTGCTCTGT
CTGCTGCTAGCTCTGCTTGTGACCATATCCGTGATTGGGTTCTTGGAACT
CCTGAGGGCACCTGGGTCTCCATGGGGGTATACTCTGATGGTTCATACAA
TGTACCAGCTGGGCTCATTTACTCATTCCCAGTAACCTGCCGCAACGGTG
AATGGACAATTGTTCAAGGACTCAGCATTGATGAATTCTCAAGGAAGAAG
TTGGACTTGACAGCAGAGGAGCTCTCCGAGGAGAAGGCCCTCGCATACTC
ATGTCTCTCTTAAATGCTTTCTTCCCTTCAATAGTGTGTTACCAAGGCCC
TTAGCTTTGTCTGAGTAGTTGGTATAGTTACAGTTTCGAATAAAACCATT
GTTGACACCTGGAGGAACCATTGATGATGGAAAAGCCTTTCCATCTTATG
TTGCTATTTCTGCATTTGAATATCGGGATTTGGGAGTTTTGGTACTTTTC
CTGGATAAATGGATATAAAGGCCCATTGGGATTTATGTATCAAACTATCA
ATAACAATTTTTTTTTTTGTTTTAACAAAAAAAGAAAAAAAATAAAAAAT
TTAAATAAACCTCAG