BQ623627

Overview
NameBQ623627
Unique NameBQ623627
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length715
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BORPA (Malate dehydrogenase OS=Bordetella parapertussis GN=mdh PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.560e-22
Identity = 46/91 (50.55%), Postives = 61/91 (67.03%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLG+  G WV+MG+ SDGSY +P G+IY FPV   NGE+ +++ L ID FSR++LD T +EL EE+
Sbjct:  232 AIIEARGLSSAASAANAAIDHVRDWVLGS-NGKWVTMGIPSDGSYGIPEGIIYGFPVVTENGEYKMIKDLEIDAFSRERLDFTLKELLEER 321

HSP 2 Score: 30.0314 bits (66), Expect = 1.560e-22
Identity = 13/28 (46.43%), Postives = 18/28 (64.29%), Query Frame = 2 
Query:   29 GEKPVRELVKDDAWLNGEFITTVQQRGA 112
            G + + +L+ DDAW    FI TV +RGA
Sbjct:  204 GGQGLTQLINDDAWNRDTFIPTVGKRGA 231
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BORBR (Malate dehydrogenase OS=Bordetella bronchiseptica GN=mdh PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.560e-22
Identity = 46/91 (50.55%), Postives = 61/91 (67.03%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLG+  G WV+MG+ SDGSY +P G+IY FPV   NGE+ +++ L ID FSR++LD T +EL EE+
Sbjct:  232 AIIEARGLSSAASAANAAIDHVRDWVLGS-NGKWVTMGIPSDGSYGIPEGIIYGFPVVTENGEYKMIKDLEIDAFSRERLDFTLKELLEER 321

HSP 2 Score: 30.0314 bits (66), Expect = 1.560e-22
Identity = 13/28 (46.43%), Postives = 18/28 (64.29%), Query Frame = 2 
Query:   29 GEKPVRELVKDDAWLNGEFITTVQQRGA 112
            G + + +L+ DDAW    FI TV +RGA
Sbjct:  204 GGQGLTQLINDDAWNRDTFIPTVGKRGA 231
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_AQUAR (Malate dehydrogenase OS=Aquaspirillum arcticum GN=mdh PE=1 SV=3)

HSP 1 Score: 97.0561 bits (240), Expect = 2.029e-22
Identity = 48/91 (52.75%), Postives = 61/91 (67.03%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII AR             DHI DWVLGT  G W +MG+ SDGSY +P G+I+ FPVT  NGE+ IVQGLSID FS++++++T  EL EE+
Sbjct:  232 AIIDARGVSSAASAANAAIDHIHDWVLGTA-GKWTTMGIPSDGSYGIPEGVIFGFPVTTENGEYKIVQGLSIDAFSQERINVTLNELLEEQ 321

HSP 2 Score: 29.6462 bits (65), Expect = 2.029e-22
Identity = 11/24 (45.83%), Postives = 17/24 (70.83%), Query Frame = 2 
Query:   41 VRELVKDDAWLNGEFITTVQQRGA 112
            V++++ DDAW    F+ TV +RGA
Sbjct:  208 VKDMINDDAWNRDTFLPTVGKRGA 231
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BURCM (Malate dehydrogenase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=mdh PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.641e-22
Identity = 48/91 (52.75%), Postives = 59/91 (64.84%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLGT  G WV+MG+ SDGSY +P  +IY  PV C NGE+  V+GL ID FSR+K+D T  EL EE+
Sbjct:  230 AIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVVCENGEYKRVEGLEIDAFSREKMDGTLAELLEER 319

HSP 2 Score: 29.261 bits (64), Expect = 2.641e-22
Identity = 15/31 (48.39%), Postives = 19/31 (61.29%), Query Frame = 2 
Query:   20 TAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            TA GE  ++ L+ DD W    FI TV +RGA
Sbjct:  200 TAEGESLLK-LINDDVWNRDTFIPTVGKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_NITEC (Malate dehydrogenase OS=Nitrosomonas eutropha (strain C91) GN=mdh PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 2.641e-22
Identity = 42/89 (47.19%), Postives = 56/89 (62.92%), Query Frame = 3 
Query:  117 IIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEE 383
            +I+AR             DH+R+W  GT +G W +MG+ SDGSY +P G+IY FPV C+NG   IVQGL I+ FSR +LD    EL++E
Sbjct:  230 VIEARGLSSAASAANAIIDHLRNWFFGTADGDWTTMGILSDGSYEIPEGVIYGFPVICKNGMREIVQGLEINPFSRTRLDAAYNELTQE 318

HSP 2 Score: 32.7278 bits (73), Expect = 2.641e-22
Identity = 14/28 (50.00%), Postives = 18/28 (64.29%), Query Frame = 2 
Query:   29 GEKPVRELVKDDAWLNGEFITTVQQRGA 112
            G+  V  L+KD  W+   FI TVQ+RGA
Sbjct:  201 GDTKVINLIKDPIWIENYFIPTVQKRGA 228
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_NOCSJ (Malate dehydrogenase OS=Nocardioides sp. (strain BAA-499 / JS614) GN=mdh PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 3.436e-22
Identity = 47/91 (51.65%), Postives = 60/91 (65.93%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             D  RDW+ G+ +  WVSM V SDGSY VP GLI SFPVT ++G+W IVQGL ID+FSR K+D +  EL++E+
Sbjct:  229 AIIEARGSSSAASAASATIDAARDWLFGSADADWVSMAVVSDGSYGVPEGLISSFPVTTKDGDWEIVQGLEIDDFSRAKIDASTAELADER 319

HSP 2 Score: 27.7202 bits (60), Expect = 3.436e-22
Identity = 12/28 (42.86%), Postives = 16/28 (57.14%), Query Frame = 2 
Query:   29 GEKPVRELVKDDAWLNGEFITTVQQRGA 112
            G +   E+V D  W+   FI TV +RGA
Sbjct:  201 GGRNAAEVVGDQDWIESTFIPTVAKRGA 228
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BURS3 (Malate dehydrogenase OS=Burkholderia sp. (strain 383) GN=mdh PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.436e-22
Identity = 47/91 (51.65%), Postives = 59/91 (64.84%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLGT  G WV+MG+ SDGSY +P  +IY  PV C NGE+  ++GL ID FSR+K+D T  EL EE+
Sbjct:  230 AIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVVCENGEYKRIEGLEIDAFSREKMDGTLAELLEER 319

HSP 2 Score: 29.261 bits (64), Expect = 3.436e-22
Identity = 15/31 (48.39%), Postives = 19/31 (61.29%), Query Frame = 2 
Query:   20 TAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            TA GE  ++ L+ DD W    FI TV +RGA
Sbjct:  200 TAEGESLLK-LINDDVWNRDTFIPTVGKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BURPS (Malate dehydrogenase OS=Burkholderia pseudomallei GN=mdh PE=1 SV=3)

HSP 1 Score: 96.6709 bits (239), Expect = 3.436e-22
Identity = 48/91 (52.75%), Postives = 59/91 (64.84%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLGT  G WV+MG+ SDGSY +P  +IY  PV C NGE+  V+GL ID FSR+K+D T  EL EE+
Sbjct:  230 AIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVICENGEYKRVEGLEIDAFSREKMDGTLAELLEER 319

HSP 2 Score: 29.261 bits (64), Expect = 3.436e-22
Identity = 15/31 (48.39%), Postives = 19/31 (61.29%), Query Frame = 2 
Query:   20 TAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            TA GE  ++ L+ DD W    FI TV +RGA
Sbjct:  200 TAEGESLLK-LINDDVWNRDTFIPTVGKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BURP6 (Malate dehydrogenase OS=Burkholderia pseudomallei (strain 668) GN=mdh PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.436e-22
Identity = 48/91 (52.75%), Postives = 59/91 (64.84%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLGT  G WV+MG+ SDGSY +P  +IY  PV C NGE+  V+GL ID FSR+K+D T  EL EE+
Sbjct:  230 AIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVICENGEYKRVEGLEIDAFSREKMDGTLAELLEER 319

HSP 2 Score: 29.261 bits (64), Expect = 3.436e-22
Identity = 15/31 (48.39%), Postives = 19/31 (61.29%), Query Frame = 2 
Query:   20 TAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            TA GE  ++ L+ DD W    FI TV +RGA
Sbjct:  200 TAEGESLLK-LINDDVWNRDTFIPTVGKRGA 229
[Close]
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Match: MDH_BURP1 (Malate dehydrogenase OS=Burkholderia pseudomallei (strain 1710b) GN=mdh PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.436e-22
Identity = 48/91 (52.75%), Postives = 59/91 (64.84%), Query Frame = 3 
Query:  114 AIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEK 386
            AII+AR             DH+RDWVLGT  G WV+MG+ SDGSY +P  +IY  PV C NGE+  V+GL ID FSR+K+D T  EL EE+
Sbjct:  230 AIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVICENGEYKRVEGLEIDAFSREKMDGTLAELLEER 319

HSP 2 Score: 29.261 bits (64), Expect = 3.436e-22
Identity = 15/31 (48.39%), Postives = 19/31 (61.29%), Query Frame = 2 
Query:   20 TAAGEKPVRELVKDDAWLNGEFITTVQQRGA 112
            TA GE  ++ L+ DD W    FI TV +RGA
Sbjct:  200 TAEGESLLK-LINDDVWNRDTFIPTVGKRGA 229
The following BLAST results are available for this feature:
BLAST of BQ623627 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 166
Match NameE-valueIdentityDescription
MDH_BORPA1.560e-2250.55Malate dehydrogenase OS=Bordetella parapertussis G... [more]
MDH_BORBR1.560e-2250.55Malate dehydrogenase OS=Bordetella bronchiseptica ... [more]
MDH_AQUAR2.029e-2252.75Malate dehydrogenase OS=Aquaspirillum arcticum GN=... [more]
MDH_BURCM2.641e-2252.75Malate dehydrogenase OS=Burkholderia ambifaria (st... [more]
MDH_NITEC2.641e-2247.19Malate dehydrogenase OS=Nitrosomonas eutropha (str... [more]
MDH_NOCSJ3.436e-2251.65Malate dehydrogenase OS=Nocardioides sp. (strain B... [more]
MDH_BURS33.436e-2251.65Malate dehydrogenase OS=Burkholderia sp. (strain 3... [more]
MDH_BURPS3.436e-2252.75Malate dehydrogenase OS=Burkholderia pseudomallei ... [more]
MDH_BURP63.436e-2252.75Malate dehydrogenase OS=Burkholderia pseudomallei ... [more]
MDH_BURP13.436e-2252.75Malate dehydrogenase OS=Burkholderia pseudomallei ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00718 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00718 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623627 ID=BQ623627; Name=BQ623627; organism=Citrus sinensis; type=EST; length=715bp
CAACCATGCAACTGTCAATACTGCAGCTGGGGAAAAGCCTGTCCGTGAGC
TTGTCAAGGATGATGCATGGTTGAATGGAGAATTCATAACCACAGTCCAA
CAACGTGGTGCTGGCAATCATCAAAGCCAGAAAGCTCTCGAGTGCTCTGT
CTGCTGCTAGCTCTGCTTGTGACCATATCCGTGATTGGGTTCTTGGAACT
CCTGAGGGCACCTGGGTCTCCATGGGGGTATACTCTGATGGTTCATACAA
TGTACCAGCTGGGCTCATTTACTCATTCCCAGTAACCTGCCGCAACGGTG
AATGGACAATTGTTCAAGGACTCAGCATTGATGAATTCTCAAGGAAGAAG
TTGGACTTGACAGCAGAGGAGCTCTCCGAGGAGAAGGCCCTCGCATACTC
ATGTCTCTCTTAAATGCTTTCTTCCCTTCAATAGTGTGTTACCAAGGCCC
TTAGCTTTGTCTGAGTAGTTGGTATAGTTACAGTTTCGAATAAAACCATT
GTTGACACCTGGAGGAACCATTGATGATGGAAAAGCCTTTCCATCTTATG
TTGCTATTTCTGCATTTGAATATCGGGATTTGGGAGTTTTGGTACTTTTC
CTGGATAAATGGATATAAAGGCCCATTGGGATTTATGTATCAAACTATCA
ATAACAATTTTTTTTTTTGTTTTAACAAAAAAAGAAAAAAAATAAAAAAT
TTAAATAAACCTCAG
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