BQ623644

Overview
NameBQ623644
Unique NameBQ623644
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length421
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: C71BE_ARATH (Cytochrome P450 71B14 OS=Arabidopsis thaliana GN=CYP71B14 PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 3.801e-25
Identity = 48/107 (44.86%), Postives = 67/107 (62.62%), Query Frame = 3 
Query:  111 IGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERF--REMAGKSDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGGDEL--VDMSEGTGLTMPR 419
            IGG+ +P+ T + VN WA+Q +P +W++P  F PERF   E+  K   + LLPFG+GRR+CPG GL M LV L L +L+  F+W    G  +  VD+ E  GL  P+
Sbjct:  376 IGGYDIPKKTWIYVNIWAVQRNPNVWKDPEVFIPERFMHSEIDYKGVDFELLPFGSGRRMCPGMGLGMALVHLTLINLLYRFDWKLPEGMNIEDVDLEESYGLVCPK 482

HSP 2 Score: 38.891 bits (89), Expect = 3.801e-25
Identity = 18/32 (56.25%), Postives = 23/32 (71.88%), Query Frame = 2 
Query:    5 DLTALPYLHGVIKETLRMYPAAPLLVPHESSE 100
            D+  L YL  VIKETLR+ P  PLL+P E+S+
Sbjct:  341 DIERLEYLKMVIKETLRINPLVPLLIPREASK 372
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: TCMO_PEA (Trans-cinnamate 4-monooxygenase OS=Pisum sativum GN=CYP73A9 PE=2 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 6.435e-25
Identity = 43/104 (41.35%), Postives = 65/104 (62.50%), Query Frame = 3 
Query:  105 APIGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFRE----MAGKSDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGGDELVDMSEGTG 404
            A +GGF +P  + +LVN+W + N+P +W++P +F+PERF E    +    + +  LPFG GRR CPG  LA+ ++G+ +G LVQ FE +   G   +D SE  G
Sbjct:  383 AKLGGFDIPAESKILVNAWWLANNPALWKKPEEFRPERFLEEEAHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKIDTSEKGG 486

HSP 2 Score: 41.9726 bits (97), Expect = 6.435e-25
Identity = 20/28 (71.43%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:    5 DLTALPYLHGVIKETLRMYPAAPLLVPH 88
            DL  LPYL  VIKETLR+  A PLLVPH
Sbjct:  350 DLEKLPYLQAVIKETLRLRMAIPLLVPH 377
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: C98A3_ARATH (Cytochrome P450 98A3 OS=Arabidopsis thaliana GN=CYP98A3 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.846e-24
Identity = 46/103 (44.66%), Postives = 62/103 (60.19%), Query Frame = 3 
Query:  111 IGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFRE--MAGKSDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGG--DELVDMSEGTGL 407
            IGG+ +P+G+ + VN WA+  DP +W+ P +F+PERF E  +  K   + LLPFG GRRVCPG  L + LV   +  L+  F W    G   E +DMSE  GL
Sbjct:  378 IGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFLEEDVDMKGHDFRLLPFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENPGL 480

HSP 2 Score: 40.817 bits (94), Expect = 1.846e-24
Identity = 15/34 (44.12%), Postives = 25/34 (73.53%), Query Frame = 2 
Query:    2 SDLTALPYLHGVIKETLRMYPAAPLLVPHESSEE 103
            +D + LPYL  V+KE+ R++P  PL++PH S+ +
Sbjct:  342 ADFSRLPYLQCVVKESFRLHPPTPLMLPHRSNAD 375
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: TCMO_POPKI (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 4.068e-24
Identity = 45/104 (43.27%), Postives = 63/104 (60.58%), Query Frame = 3 
Query:  105 APIGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFREMAGK----SDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGGDELVDMSEGTG 404
            A +GGF +P  + +LVN+W + N+P  W+ P +F+PERF E   K     + +  LPFG GRR CPG  LA+ ++G+ LG LVQ FE +   G   +D SE  G
Sbjct:  383 AKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPERFLEEGAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTSEKGG 486

HSP 2 Score: 39.6614 bits (91), Expect = 4.068e-24
Identity = 18/28 (64.29%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:    5 DLTALPYLHGVIKETLRMYPAAPLLVPH 88
            D   LPYL+ V+KETLR+  A PLLVPH
Sbjct:  350 DTYKLPYLNAVVKETLRLRMAIPLLVPH 377
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: TCMO_POPTM (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.294e-24
Identity = 45/104 (43.27%), Postives = 63/104 (60.58%), Query Frame = 3 
Query:  105 APIGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFREMAGK----SDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGGDELVDMSEGTG 404
            A +GGF +P  + +LVN+W + N+P  W+ P +F+PERF E   K     + +  LPFG GRR CPG  LA+ ++G+ LG LVQ FE +   G   +D SE  G
Sbjct:  383 AKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPERFLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTSEKGG 486

HSP 2 Score: 39.6614 bits (91), Expect = 5.294e-24
Identity = 18/28 (64.29%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:    5 DLTALPYLHGVIKETLRMYPAAPLLVPH 88
            D   LPYL+ V+KETLR+  A PLLVPH
Sbjct:  350 DTYKLPYLNAVVKETLRLRMAIPLLVPH 377
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: TCMO_ZINEL (Trans-cinnamate 4-monooxygenase OS=Zinnia elegans GN=CYP73A12 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 5.294e-24
Identity = 43/104 (41.35%), Postives = 65/104 (62.50%), Query Frame = 3 
Query:  105 APIGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFREMAGK----SDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGGDELVDMSEGTG 404
            A +GG+ +P  + +LVN+W + N+P  W++P +F+PERF E   K     + +  LPFG GRR CPG  LA+ ++G+ +G LVQ FE +   G + VD +E  G
Sbjct:  383 AKLGGYDIPAESKILVNAWWLANNPDQWKKPEEFRPERFLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQDKVDTTEKGG 486

HSP 2 Score: 41.5874 bits (96), Expect = 5.294e-24
Identity = 20/28 (71.43%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:    5 DLTALPYLHGVIKETLRMYPAAPLLVPH 88
            DL  LPYL  VIKETLR+  A PLLVPH
Sbjct:  350 DLHKLPYLQAVIKETLRLRMAIPLLVPH 377
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: C98A2_SOYBN (Cytochrome P450 98A2 OS=Glycine max GN=CYP98A2 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 8.963e-24
Identity = 45/103 (43.69%), Postives = 63/103 (61.17%), Query Frame = 3 
Query:  111 IGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFRE--MAGKSDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGG--DELVDMSEGTGL 407
            +GG+ +P+G+ + VN WA+  DP +W++P +F+PERF E  +  K   + LLPFG+GRRVCPG  L + L    LG L+  F W    G   E +DM E  GL
Sbjct:  379 VGGYDIPKGSNVHVNVWAVARDPAVWKDPLEFRPERFLEEDVDMKGHDFRLLPFGSGRRVCPGAQLGINLAASMLGHLLHHFCWTPPEGMKPEEIDMGENPGL 481

HSP 2 Score: 37.7354 bits (86), Expect = 8.963e-24
Identity = 14/32 (43.75%), Postives = 23/32 (71.88%), Query Frame = 2 
Query:    2 SDLTALPYLHGVIKETLRMYPAAPLLVPHESS 97
            +D + LPYL  V KE +R++P  PL++PH ++
Sbjct:  343 ADFSNLPYLQCVTKEAMRLHPPTPLMLPHRAN 374
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: TCMO_SOYBN (Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 8.964e-24
Identity = 43/104 (41.35%), Postives = 64/104 (61.54%), Query Frame = 3 
Query:  105 APIGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFRE----MAGKSDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGGDELVDMSEGTG 404
            A +GG+ +P  + +LVN+W + N+P  W++P +F+PERF E    +    + +  LPFG GRR CPG  LA+ ++G+ LG LVQ FE +   G   +D SE  G
Sbjct:  384 AKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPERFFEEESLVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQIDTSEKGG 487

HSP 2 Score: 40.817 bits (94), Expect = 8.964e-24
Identity = 18/28 (64.29%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:    5 DLTALPYLHGVIKETLRMYPAAPLLVPH 88
            D+  LPYL  V+KETLR+  A PLLVPH
Sbjct:  351 DIQKLPYLQAVVKETLRLRMAIPLLVPH 378
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: TCMO_PHAAU (Trans-cinnamate 4-monooxygenase OS=Phaseolus aureus GN=CYP73A2 PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 8.964e-24
Identity = 43/104 (41.35%), Postives = 64/104 (61.54%), Query Frame = 3 
Query:  105 APIGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFRE----MAGKSDKYMLLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGGDELVDMSEGTG 404
            A +GG+ +P  + +LVN+W + N+P  W++P +F+PERF E    +    + +  LPFG GRR CPG  LA+ ++G+ LG LVQ FE +   G   +D SE  G
Sbjct:  383 AKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPERFFEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQIDTSEKGG 486

HSP 2 Score: 40.817 bits (94), Expect = 8.964e-24
Identity = 18/28 (64.29%), Postives = 21/28 (75.00%), Query Frame = 2 
Query:    5 DLTALPYLHGVIKETLRMYPAAPLLVPH 88
            D+  LPYL  V+KETLR+  A PLLVPH
Sbjct:  350 DIQKLPYLQAVVKETLRLRMAIPLLVPH 377
[Close]
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Match: C71C2_MAIZE (Cytochrome P450 71C2 OS=Zea mays GN=CYP71C2 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.165e-23
Identity = 43/109 (39.45%), Postives = 61/109 (55.96%), Query Frame = 3 
Query:  111 IGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFREMAGKSDKYM------LLPFGTGRRVCPGEGLAMRLVGLALGSLVQCFEWVRTGGDELVDMSEGTGLTMPR 419
            I G+ VP GT +LV++WA+  D   WE+P +F PERF +  G  D +M       +PFG+GRR+CPG       + + L +L+  F+W   G    V M E  GLT+ R
Sbjct:  416 IDGYFVPAGTRVLVHAWALGRDRTTWEKPEEFMPERFVQEPGAVDVHMKGKDLRFIPFGSGRRICPGMNFGFATMEVMLANLMYHFDWEVPGSGAGVSMEESFGLTLRR 524

HSP 2 Score: 43.5134 bits (101), Expect = 1.165e-23
Identity = 18/33 (54.55%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:    5 DLTALPYLHGVIKETLRMYPAAPLLVPHESSEE 103
            DL ++PYL   IKETLR++P AP L+PH S+ +
Sbjct:  381 DLGSMPYLKATIKETLRLHPPAPFLLPHYSTAD 413
The following BLAST results are available for this feature:
BLAST of BQ623644 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 262
Match NameE-valueIdentityDescription
C71BE_ARATH3.801e-2544.86Cytochrome P450 71B14 OS=Arabidopsis thaliana GN=C... [more]
TCMO_PEA6.435e-2541.35Trans-cinnamate 4-monooxygenase OS=Pisum sativum G... [more]
C98A3_ARATH1.846e-2444.66Cytochrome P450 98A3 OS=Arabidopsis thaliana GN=CY... [more]
TCMO_POPKI4.068e-2443.27Trans-cinnamate 4-monooxygenase OS=Populus kitakam... [more]
TCMO_POPTM5.294e-2443.27Trans-cinnamate 4-monooxygenase OS=Populus tremulo... [more]
TCMO_ZINEL5.294e-2441.35Trans-cinnamate 4-monooxygenase OS=Zinnia elegans ... [more]
C98A2_SOYBN8.963e-2443.69Cytochrome P450 98A2 OS=Glycine max GN=CYP98A2 PE=... [more]
TCMO_SOYBN8.964e-2441.35Trans-cinnamate 4-monooxygenase OS=Glycine max GN=... [more]
TCMO_PHAAU8.964e-2441.35Trans-cinnamate 4-monooxygenase OS=Phaseolus aureu... [more]
C71C2_MAIZE1.165e-2339.45Cytochrome P450 71C2 OS=Zea mays GN=CYP71C2 PE=2 S... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00735 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00735 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623644 ID=BQ623644; Name=BQ623644; organism=Citrus sinensis; type=EST; length=421bp
GTCTGATCTTACCGCACTTCCATATCTCCACGGCGTCATCAAAGAGACAC
TCAGGATGTACCCTGCAGCTCCACTACTAGTTCCTCATGAATCGTCCGAG
GAGTGCACCTATAGGAGGTTTCAAAGTTCCACGTGGCACAATGTTGTTGG
TGAACTCGTGGGCCATACAAAATGATCCTAGAATATGGGAAGAGCCCTCA
AAATTCAAGCCAGAGAGGTTCCGGGAAATGGCAGGGAAAAGTGATAAGTA
TATGCTTTTGCCATTTGGGACAGGGAGGAGAGTGTGTCCTGGAGAAGGCT
TGGCGATGAGGTTGGTTGGGTTGGCATTAGGTTCGTTGGTTCAGTGCTTT
GAGTGGGTGAGAACAGGTGGTGATGAGTTGGTGGACATGAGCGAAGGCAC
TGGGCTTACCATGCCTAGATC
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