BQ623646 | Citrus Genome Database

Overview


Name	BQ623646
Unique Name	BQ623646
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	591

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2108	contig	Csv1_Contig2108:161..750.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

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BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_TOLAT (Flavoprotein wrbA OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=wrbA PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 4.006e-25
Identity = 46/104 (44.23%), Postives = 55/104 (52.88%), Query Frame = 1

Query:   91 KSDVPIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            +   P+ TP EL + D  +FG PTRFG M AQ + FLD TGGLW    L GK A +F               T  T L HHGM+ VPIGY   A MF++  V G
Sbjct:   56 EQSAPVATPAELVDYDAIIFGVPTRFGNMTAQMRNFLDQTGGLWAKGALFGKIASVFASTGVGGGQEMTITST-WTTLAHHGMVIVPIGYG-TAEMFDISHVGG 157

HSP 2 Score: 52.7582 bits (125), Expect = 4.006e-25
Identity = 27/43 (62.79%), Postives = 31/43 (72.09%), Query Frame = 2

Query:  401 GGSPYGAGTFAG-DGSRQPTELELEQAFHQGKHIAGIAKKLKG 526
            GG+PYGA T AG DGSRQP   EL  A  QGKH+A +A K+KG
Sbjct:  157 GGTPYGATTLAGGDGSRQPDARELAIARFQGKHVATVAAKMKG 199

HSP 3 Score: 20.4014 bits (41), Expect = 4.006e-25
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 3

Query:    9 VEGVEAKLWQVPETL 53
            V GVE  + +VPET+
Sbjct:   29 VSGVEVTIKRVPETM 43

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_AZOSB (Flavoprotein wrbA OS=Azoarcus sp. (strain BH72) GN=wrbA PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 5.042e-25
Identity = 44/117 (37.61%), Postives = 60/117 (51.28%), Query Frame = 1

Query:   52 LPEEVLGKMSAPPKSDVPIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            +PEEV  K         P+ T  EL + D  +FG PTRFG MA+Q + FLD TGGLW + +L GK   +F               +  T L+HHGM+ V + YT  A    M+++ G
Sbjct:   42 VPEEVARKSGMKLDQPAPVATVGELPDYDAIIFGTPTRFGNMASQMRNFLDQTGGLWMSGKLVGKVGSVFASTATQHGGQETTITSFHTTLLHHGMVIVGLPYT-EARQMNMDEITG 157

HSP 2 Score: 52.7582 bits (125), Expect = 5.042e-25
Identity = 28/43 (65.12%), Postives = 31/43 (72.09%), Query Frame = 2

Query:  401 GGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHIAGIAKKLKG 526
            GGSPYGA T A GDG+RQP+E EL  A  QG H+A IA KL G
Sbjct:  157 GGSPYGATTLAKGDGTRQPSENELAMARFQGGHVAKIAAKLAG 199

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_MARAV (Flavoprotein wrbA OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=wrbA PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 8.540e-25
Identity = 46/124 (37.10%), Postives = 66/124 (53.23%), Query Frame = 1

Query:   43 LRHLPEEVLGK--MSAPPKSD--VPIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            ++ +PE +  +  ++A  K+D   P+  P ELA+ D  +FG PTRFG MA Q + FLD TGGLW   +L GK  G  +              +  T L HHGM+ VP+GY      F++ +V G
Sbjct:   36 VKRVPETMADEAFLNAGGKADQGAPVADPKELADYDAIIFGTPTRFGNMAGQMRTFLDQTGGLWAEGKLHGK-VGSVFTSTGTGGGQEQTITSFWTTLAHHGMVLVPLGYGI-PEFFDISEVNG 157

HSP 2 Score: 53.9138 bits (128), Expect = 8.540e-25
Identity = 28/43 (65.12%), Postives = 32/43 (74.42%), Query Frame = 2

Query:  401 GGSPYGAGTFAG-DGSRQPTELELEQAFHQGKHIAGIAKKLKG 526
            GG+PYGA T AG DGSRQP+E EL  A  QGKH+A +A KL G
Sbjct:  157 GGTPYGASTIAGGDGSRQPSEKELAIARFQGKHVAELAIKLHG 199

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_HALHL (Flavoprotein wrbA OS=Halorhodospira halophila (strain DSM 244 / SL1) GN=wrbA PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.883e-24
Identity = 44/121 (36.36%), Postives = 59/121 (48.76%), Query Frame = 1

Query:   52 LPEEVLGKMSAPPKSDVPIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQ----LVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            +PE+V  +  A    + P+ +  ELA+ D  +FG PTRFG MA Q + FLD TG LW   QL GK   +F               T IT     L HHGM+ V + Y     + ++  VKG
Sbjct:   42 MPEDVARQAGAKLDQEAPVASLEELADYDAILFGTPTRFGNMAGQMRNFLDQTGSLWFNGQLIGKVGSVFTSTGTGGGNE-----TTITSFWHTLAHHGMVIVGLSYA-APELADLSVVKG 156

HSP 2 Score: 60.4622 bits (145), Expect = 1.883e-24
Identity = 30/44 (68.18%), Postives = 35/44 (79.55%), Query Frame = 2

Query:  398 KGGSPYGAGTFAG-DGSRQPTELELEQAFHQGKHIAGIAKKLKG 526
            KGGSPYGAGT AG DGSRQP E EL  A +QG+H+A IA++L G
Sbjct:  155 KGGSPYGAGTIAGGDGSRQPNEQELALARYQGRHVAEIAQRLHG 198

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_RHOCS (Flavoprotein wrbA OS=Rhodospirillum centenum (strain ATCC 51521 / SW) GN=wrbA PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.450e-24
Identity = 44/117 (37.61%), Postives = 58/117 (49.57%), Query Frame = 1

Query:   52 LPEEVLGKMSAPPKSDVPIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            +PEEV            P+ T  ELAE D  + G PTRFG M +Q   FLD TGGLW    L GK    F               + +T L+HHGM+ V + Y++  G+  +E+VKG
Sbjct:   43 VPEEVARNAGMKLDQPAPVATVAELAEYDAIIVGSPTRFGRMTSQMANFLDQTGGLWAKGALNGKVGAAFTSTASQHGGQETTLFSILTNLLHHGMVVVGLPYSY-EGLSGVEQVKG 158

HSP 2 Score: 52.373 bits (124), Expect = 2.450e-24
Identity = 27/44 (61.36%), Postives = 33/44 (75.00%), Query Frame = 2

Query:  398 KGGSPYGAGTFA-GDGSRQPTELELEQAFHQGKHIAGIAKKLKG 526
            KGG+PYGA T A GDGSR+PTE+EL  A +QG  +A  A KL+G
Sbjct:  157 KGGTPYGASTIADGDGSRRPTEVELGGARYQGALVARTAAKLRG 200

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: PST2_YEAST (Protoplast secreted protein 2 OS=Saccharomyces cerevisiae GN=PST2 PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.189e-24
Identity = 51/118 (43.22%), Postives = 65/118 (55.08%), Query Frame = 1

Query:   52 LPEEVLGKMSAPPKSDVPIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGY-TFGAGMFEMEKVKG 402
            L  EV+  +   PK D PI T + L E D F+FG PTRFG   AQ+KAF D TGGLW    L GK AG F              + +++ L HHG+IFVP+GY    A +  M++V G
Sbjct:   42 LSPEVVKALGGAPKPDYPIATQDTLTEYDAFLFGIPTRFGNFPAQWKAFWDRTGGLWAKGALHGKVAGCF-VSTGTGGGNEATIMNSLSTLAHHGIIFVPLGYKNVFAELTNMDEVHG 158

HSP 2 Score: 40.4318 bits (93), Expect = 3.189e-24
Identity = 21/32 (65.62%), Postives = 24/32 (75.00%), Query Frame = 2

Query:  401 GGSPYGAGTFAG-DGSRQPTELELEQAFHQGK 493
            GGSP+GAGT AG DGSR P+ LEL+    QGK
Sbjct:  158 GGSPWGAGTIAGSDGSRSPSALELQVHEIQGK 189

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_SYNFM (Flavoprotein wrbA OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) GN=wrbA PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 5.408e-24
Identity = 47/128 (36.72%), Postives = 65/128 (50.78%), Query Frame = 1

Query:   43 LRHLPE----EVLGKMSAPPK----SDVPIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            LR +PE    E+L  M A       + +P+ T +EL  AD  +FG PTRFG MA Q + FLDATG LW +  L GK   +F              L+    L+H GM+ V + Y+F  G   M+++ G
Sbjct:   35 LRRVPETLSAEILASMGATEAQKQFAHIPVCTVDELGAADAVIFGTPTRFGNMAGQMRQFLDATGRLWVSGALVGKAGSVFTSSNTQHGGQESTILSFHINLLHQGMVIVGLPYSF-QGQSTMDEITG 161

HSP 2 Score: 48.521 bits (114), Expect = 5.408e-24
Identity = 24/41 (58.54%), Postives = 31/41 (75.61%), Query Frame = 2

Query:  401 GGSPYGAGTFAGD-GSRQPTELELEQAFHQGKHIAGIAKKL 520
            GGSPYGA T AG  G+R P++ EL  A +QG+H+A IA+KL
Sbjct:  161 GGSPYGASTIAGPTGARTPSDNELAGARYQGRHVAEIARKL 201

HSP 3 Score: 24.2534 bits (51), Expect = 5.408e-24
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 3

Query:    6 SVEGVEAKLWQVPETLT 56
            SV+G EA L +VPETL+
Sbjct:   27 SVDGAEAVLRRVPETLS 43

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_GEOUR (Flavoprotein wrbA OS=Geobacter uraniireducens (strain Rf4) GN=wrbA PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 7.025e-24
Identity = 47/121 (38.84%), Postives = 60/121 (49.59%), Query Frame = 1

Query:   52 LPEEVLGKMSA----PPKSDVPIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            LP +VL KM A       + +P+ T  ELA+AD  +FG PTRFG M  Q + FLDATGGLW    L GK   +F              L+    L+H GM+ V + Y F   M   E + G
Sbjct:   42 LPADVLEKMGAVETQKKMAQIPVCTIGELADADAIIFGTPTRFGNMCGQMRQFLDATGGLWMKGSLVGKLGSVFTSSATQHGGQESTILSFHITLLHQGMVVVGLPYAFQGQMRNDEIIGG 162

HSP 2 Score: 48.9062 bits (115), Expect = 7.025e-24
Identity = 24/41 (58.54%), Postives = 29/41 (70.73%), Query Frame = 2

Query:  401 GGSPYGAGTFAG-DGSRQPTELELEQAFHQGKHIAGIAKKL 520
            GGSPYGA T AG  G R P+E +L  A +QGKH+A IA +L
Sbjct:  161 GGSPYGASTIAGTQGERSPSENDLAAARYQGKHVASIAARL 201

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_BURVG (Flavoprotein wrbA OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=wrbA PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 7.027e-24
Identity = 39/100 (39.00%), Postives = 54/100 (54.00%), Query Frame = 1

Query:  103 PIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            P+ T +ELA  D  +FG PTRFG MA Q + FLD TGGLW    L GK   +F               +  T L+HHGM+ V + Y   +G+ +M+++ G
Sbjct:   60 PVATVDELANYDAIIFGTPTRFGNMAGQMRTFLDQTGGLWMKGALVGKIGSVFASTGTQHGGQETTITSFHTTLLHHGMVIVGVPYAC-SGLVDMKEITG 158

HSP 2 Score: 51.6026 bits (122), Expect = 7.027e-24
Identity = 26/41 (63.41%), Postives = 32/41 (78.05%), Query Frame = 2

Query:  401 GGSPYGAGTFAG-DGSRQPTELELEQAFHQGKHIAGIAKKL 520
            GG+PYGA T AG DGSRQP+  EL+ A +QGKH+A +A KL
Sbjct:  158 GGTPYGATTLAGADGSRQPSANELDIARYQGKHVAQLAAKL 198

HSP 3 Score: 22.3274 bits (46), Expect = 7.027e-24
Identity = 10/16 (62.50%), Postives = 12/16 (75.00%), Query Frame = 3

Query:    6 SVEGVEAKLWQVPETL 53
            SV GVE  L +VPET+
Sbjct:   28 SVPGVEVTLKRVPETI 43

[Close]

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Match: WRBA_BURCJ (Flavoprotein wrbA OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=wrbA PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 7.027e-24
Identity = 39/100 (39.00%), Postives = 53/100 (53.00%), Query Frame = 1

Query:  103 PIITPNELAEADGFVFGFPTRFGMMAAQFKAFLDATGGLWRTQQLAGKPAGMFYXXXXXXXXXXXXALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKG 402
            P+ T +ELA+ D  +FG PTRFG MA Q + FLD TGGLW    L GK   +F               +  T L+HHGM+ V + Y   +G+  M ++ G
Sbjct:   60 PVATVDELADYDAIIFGTPTRFGNMAGQMRTFLDQTGGLWMKGALVGKIGSVFASTGTQHGGQETTITSFHTTLLHHGMVIVGVPYAC-SGLVNMNEITG 158

HSP 2 Score: 51.9878 bits (123), Expect = 7.027e-24
Identity = 26/41 (63.41%), Postives = 32/41 (78.05%), Query Frame = 2

Query:  401 GGSPYGAGTFAG-DGSRQPTELELEQAFHQGKHIAGIAKKL 520
            GG+PYGA T AG DGSRQP+  EL+ A +QGKH+A +A KL
Sbjct:  158 GGTPYGATTLAGADGSRQPSANELDIARYQGKHVAELANKL 198

HSP 3 Score: 22.3274 bits (46), Expect = 7.027e-24
Identity = 10/16 (62.50%), Postives = 12/16 (75.00%), Query Frame = 3

Query:    6 SVEGVEAKLWQVPETL 53
            SV GVE  L +VPET+
Sbjct:   28 SVPGVEVTLKRVPETI 43

The following BLAST results are available for this feature:

BLAST of BQ623646 vs. ExPASy Swiss-Prot

BLAST of BQ623646 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 146


Match Name	E-value	Identity	Description
WRBA_TOLAT	4.006e-25	44.23	Flavoprotein wrbA OS=Tolumonas auensis (strain DSM...	[more]
WRBA_AZOSB	5.042e-25	37.61	Flavoprotein wrbA OS=Azoarcus sp. (strain BH72) GN...	[more]
WRBA_MARAV	8.540e-25	37.10	Flavoprotein wrbA OS=Marinobacter aquaeolei (strai...	[more]
WRBA_HALHL	1.883e-24	36.36	Flavoprotein wrbA OS=Halorhodospira halophila (str...	[more]
WRBA_RHOCS	2.450e-24	37.61	Flavoprotein wrbA OS=Rhodospirillum centenum (stra...	[more]
PST2_YEAST	3.189e-24	43.22	Protoplast secreted protein 2 OS=Saccharomyces cer...	[more]
WRBA_SYNFM	5.408e-24	36.72	Flavoprotein wrbA OS=Syntrophobacter fumaroxidans ...	[more]
WRBA_GEOUR	7.025e-24	38.84	Flavoprotein wrbA OS=Geobacter uraniireducens (str...	[more]
WRBA_BURVG	7.027e-24	39.00	Flavoprotein wrbA OS=Burkholderia vietnamiensis (s...	[more]
WRBA_BURCJ	7.027e-24	39.00	Flavoprotein wrbA OS=Burkholderia cepacia (strain ...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_00737 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00737 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623646 ID=BQ623646; Name=BQ623646; organism=Citrus sinensis; type=EST; length=591bp
CTTCATCTGTTGAGGGTGTTGAAGCCAAATTATGGCAGGTCCCTGAGACA
CTTACCTGAGGAGGTACTTGGTAAGATGAGTGCACCACCAAAGAGTGATG
TACCTATTATTACCCCCAACGAACTTGCTGAGGCTGATGGGTTTGTTTTT
GGCTTCCCAACAAGATTTGGAATGATGGCTGCTCAATTTAAAGCATTTTT
AGATGCTACTGGTGGTCTATGGAGGACACAACAGCTCGCTGGCAAACCTG
CTGGGATGTTCTACAGCACTGGGTCACAAGGTGGTGGACAAGAGACTACA
GCGTTGACGGCAATTACTCAGCTTGTTCACCATGGGATGATATTTGTTCC
TATTGGATACACATTTGGTGCTGGCATGTTTGAGATGGAGAAAGTGAAAG
GGTGGAAGTCCCTACGGTGCAGGAACTTTTGCTGGAGATGGCTCGAGACA
GCCAACTGAGCTTGAGTTGGAGCAAGCTTTCCACCAGGGAAAACACATTG
CGGGCATTGCAAAGAAGCTGAAGGGAAGTGCTTAAATATTTCAATTTACA
ATACACCATTAGGTGGACTTATTCCATTCTTTTCGTCTCTA