BQ623746

Overview
NameBQ623746
Unique NameBQ623746
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length587
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:1015..1600. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_ORYSJ (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. japonica GN=SAM1 PE=2 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 2.318e-57
Identity = 88/98 (89.80%), Postives = 95/98 (96.94%), Query Frame = 3 
Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            +G ARRCIVQVSYAIGVPEPLSVFVD+YGTG+IPDKEILKIVKE+FDFRPGM+ INLDLK+GGNGR+LKTAAYGHFGRDDPDFTWEVVKPLKWEKP A
Sbjct:  299 SGLARRCIVQVSYAIGVPEPLSVFVDTYGTGRIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKWEKPSA 396

HSP 2 Score: 51.6026 bits (122), Expect = 2.318e-57
Identity = 26/42 (61.90%), Postives = 28/42 (66.67%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAY+ RQAAKSIVA+GL
Sbjct:  260 DTYGGWGAHGGGAFSGKDPTKVDRSGAYVARQAAKSIVASGL 301
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_ORYSI (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. indica GN=SAM1 PE=2 SV=2)

HSP 1 Score: 191.815 bits (486), Expect = 2.318e-57
Identity = 88/98 (89.80%), Postives = 95/98 (96.94%), Query Frame = 3 
Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            +G ARRCIVQVSYAIGVPEPLSVFVD+YGTG+IPDKEILKIVKE+FDFRPGM+ INLDLK+GGNGR+LKTAAYGHFGRDDPDFTWEVVKPLKWEKP A
Sbjct:  299 SGLARRCIVQVSYAIGVPEPLSVFVDTYGTGRIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKWEKPSA 396

HSP 2 Score: 51.6026 bits (122), Expect = 2.318e-57
Identity = 26/42 (61.90%), Postives = 28/42 (66.67%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAY+ RQAAKSIVA+GL
Sbjct:  260 DTYGGWGAHGGGAFSGKDPTKVDRSGAYVARQAAKSIVASGL 301
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK_DENCR (S-adenosylmethionine synthetase OS=Dendrobium crumenatum GN=SAMS PE=2 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 3.022e-57
Identity = 89/97 (91.75%), Postives = 94/97 (96.91%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+TINLDLKRGGN RF+KTAAYGHFGRDDPDFTWEVVKPLKW+KP A
Sbjct:  300 GLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMITINLDLKRGGN-RFIKTAAYGHFGRDDPDFTWEVVKPLKWDKPAA 395

HSP 2 Score: 55.0694 bits (131), Expect = 3.022e-57
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKS+VANGL
Sbjct:  260 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVANGL 301
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK2_BETVU (S-adenosylmethionine synthetase 2 OS=Beta vulgaris GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 3.939e-57
Identity = 88/94 (93.62%), Postives = 91/94 (96.81%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 404
            G ARR IVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMM+INLDLKRGGNGRF KTAAYGHFGRDDPDFTWEVVKPLKWEK
Sbjct:  297 GLARRAIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMMSINLDLKRGGNGRFQKTAAYGHFGRDDPDFTWEVVKPLKWEK 390

HSP 2 Score: 55.4546 bits (132), Expect = 3.939e-57
Identity = 29/42 (69.05%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVANGL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_PETCR (S-adenosylmethionine synthetase 1 (Fragment) OS=Petroselinum crispum GN=SMS-1 PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 3.961e-57
Identity = 87/95 (91.58%), Postives = 94/95 (98.95%), Query Frame = 3 
Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 404
            +G ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGR+DPDFTWEVVKPLKWEK
Sbjct:  139 SGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 233

HSP 2 Score: 53.5286 bits (127), Expect = 3.961e-57
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVA+GL
Sbjct:  100 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGL 141
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK2_PETCR (S-adenosylmethionine synthetase 2 (Fragment) OS=Petroselinum crispum GN=SMS-2 PE=2 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 5.209e-57
Identity = 87/95 (91.58%), Postives = 94/95 (98.95%), Query Frame = 3 
Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 404
            +G ARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL+IVKE+FDFRPGM++INLDLKRGGNGRFLKTAAYGHFGR+DPDFTWEVVKPLKWEK
Sbjct:   50 SGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 144

HSP 2 Score: 53.5286 bits (127), Expect = 5.209e-57
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVA+GL
Sbjct:   11 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGL 52
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK_GOSHI (S-adenosylmethionine synthetase OS=Gossypium hirsutum GN=SAMS PE=2 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 1.934e-56
Identity = 86/97 (88.66%), Postives = 91/97 (93.81%), Query Frame = 3 
Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRC+VQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+ INLDLKRGGN RFLKTAAYGHFGRDD DFTWEVVKPLKW+K  A
Sbjct:  297 GLARRCVVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLKRGGNSRFLKTAAYGHFGRDDTDFTWEVVKPLKWDKVHA 393

HSP 2 Score: 55.4546 bits (132), Expect = 1.934e-56
Identity = 29/42 (69.05%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVANGL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK4_VITVI (S-adenosylmethionine synthetase 4 OS=Vitis vinifera GN=METK4 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 2.522e-56
Identity = 89/97 (91.75%), Postives = 92/97 (94.85%), Query Frame = 3 
Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 410
            +G ARRCIVQVSYAIGVPEPLSVFVD+Y TGKIPDKEILKIVKESFDFRPGM+ INLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLK EK Q
Sbjct:  296 SGLARRCIVQVSYAIGVPEPLSVFVDTYATGKIPDKEILKIVKESFDFRPGMIAINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKAEKAQ 392

HSP 2 Score: 53.5286 bits (127), Expect = 2.522e-56
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVA+GL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK2_POPTR (S-adenosylmethionine synthetase 2 OS=Populus trichocarpa GN=METK2 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 2.522e-56
Identity = 88/97 (90.72%), Postives = 92/97 (94.85%), Query Frame = 3 
Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQ 410
            +G ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL IVKE FDFRPGM+ I+LDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLK EKPQ
Sbjct:  296 SGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKEKFDFRPGMIAISLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKSEKPQ 392

HSP 2 Score: 53.5286 bits (127), Expect = 2.522e-56
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVA+GL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGL 298
[Close]
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK5_POPTR (S-adenosylmethionine synthetase 5 OS=Populus trichocarpa GN=METK5 PE=2 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 3.288e-56
Identity = 86/94 (91.49%), Postives = 92/94 (97.87%), Query Frame = 3 
Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWE 401
            +G ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL+IVKESFDFRPGM++INLDLKRGGN RFLKTAAYGHFGRDDPDFTWEVVKPLKW+
Sbjct:  297 SGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILQIVKESFDFRPGMISINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWEVVKPLKWD 390

HSP 2 Score: 53.5286 bits (127), Expect = 3.288e-56
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2 
Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVA+GL
Sbjct:  258 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGL 299
The following BLAST results are available for this feature:
BLAST of BQ623746 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_ORYSJ2.318e-5789.80S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK1_ORYSI2.318e-5789.80S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK_DENCR3.022e-5791.75S-adenosylmethionine synthetase OS=Dendrobium crum... [more]
METK2_BETVU3.939e-5793.62S-adenosylmethionine synthetase 2 OS=Beta vulgaris... [more]
METK1_PETCR3.961e-5791.58S-adenosylmethionine synthetase 1 (Fragment) OS=Pe... [more]
METK2_PETCR5.209e-5791.58S-adenosylmethionine synthetase 2 (Fragment) OS=Pe... [more]
METK_GOSHI1.934e-5688.66S-adenosylmethionine synthetase OS=Gossypium hirsu... [more]
METK4_VITVI2.522e-5691.75S-adenosylmethionine synthetase 4 OS=Vitis vinifer... [more]
METK2_POPTR2.522e-5690.72S-adenosylmethionine synthetase 2 OS=Populus trich... [more]
METK5_POPTR3.288e-5691.49S-adenosylmethionine synthetase 5 OS=Populus trich... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00837 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00837 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623746 ID=BQ623746; Name=BQ623746; organism=Citrus sinensis; type=EST; length=587bp
GGACACTTATGGTGGCTGGGGAGCCCATGGTGGCGGTGCTTTCTCTGGAA
AAGACCCTACTAAGGTGGATAGGAGTGGTGCATACATTGTTAGGCAGGCT
GCTAAGAGCATTGTTGCAAACGGGCTTTGCTCGCAGGTGCATTGTCCAAG
TCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCTGTTTTTGTGGACTCA
TATGGAACCGGCAAGATTCCTGACAAGGAGATCCTCAAAATTGTGAAGGA
AAGCTTTGATTTCAGGCCAGGAATGATGACCATCAATTTGGATCTCAAAA
GGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCCTATGGACACTTTGGC
AGGGATGACCCAGACTTCACTTGGGAAGTTGTGAAGCCCCTCAAGTGGGA
AAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAAAACTATCCCTGCTGT
TCAAGTTTGCTTCTATTGGTAGTGGATGAATAATTTGAATATTATCTTGC
TGCTGCTTTGTAATACTAAGCAAATCTCCTGGGTTCAAAACTCATACATG
TTCCCCATTCAATTCCCTTTCTTATTCTTCTTTCCCC
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