BQ623746 | Citrus Genome Database

Overview


Name	BQ623746
Unique Name	BQ623746
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	587

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig1234	contig	Csv1_Contig1234:1015..1600.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK2_ATRNU (S-adenosylmethionine synthetase 2 OS=Atriplex nummularia GN=SAMS2 PE=1 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 4.228e-53
Identity = 84/97 (86.60%), Postives = 91/97 (93.81%), Query Frame = 3

Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+ INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEKPQA
Sbjct:  301 GLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396

HSP 2 Score: 50.8322 bits (120), Expect = 4.228e-53
Identity = 27/42 (64.29%), Postives = 27/42 (64.29%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYI RQAAKSIVA GL
Sbjct:  261 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGL 302

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_ATRNU (S-adenosylmethionine synthetase 1 OS=Atriplex nummularia GN=SAMS1 PE=1 SV=2)

HSP 1 Score: 178.333 bits (451), Expect = 4.228e-53
Identity = 84/97 (86.60%), Postives = 91/97 (93.81%), Query Frame = 3

Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            G ARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGM+ INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEKPQA
Sbjct:  301 GLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396

HSP 2 Score: 50.8322 bits (120), Expect = 4.228e-53
Identity = 27/42 (64.29%), Postives = 27/42 (64.29%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYI RQAAKSIVA GL
Sbjct:  261 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGL 302

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK3_PICSI (S-adenosylmethionine synthetase 3 OS=Picea sitchensis GN=METK3 PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 5.511e-53
Identity = 81/94 (86.17%), Postives = 89/94 (94.68%), Query Frame = 3

Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 404
            G ARRC+VQVSYAIGVPEPLSVFVDSYGTG IPDK IL+++K++FDFRPGM+TINLDLKRGGNGRF KTAAYGHFGRDDPDFTWE+VKPLK EK
Sbjct:  297 GLARRCLVQVSYAIGVPEPLSVFVDSYGTGTIPDKVILELIKKNFDFRPGMITINLDLKRGGNGRFQKTAAYGHFGRDDPDFTWEIVKPLKQEK 390

HSP 2 Score: 52.373 bits (124), Expect = 5.511e-53
Identity = 28/42 (66.67%), Postives = 28/42 (66.67%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVA GL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVAAGL 298

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_HORVU (S-adenosylmethionine synthetase 1 OS=Hordeum vulgare GN=SAM1 PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 9.365e-53
Identity = 83/98 (84.69%), Postives = 93/98 (94.90%), Query Frame = 3

Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            +G ARRCIVQ+SYAIGVPEPLSVFVDSYGTGKIPD+EILK+VKE+FDFRPGM+TINLDLK+GGN RF+KTAAYGHFGRDD DFTWEVVKPLK++K  A
Sbjct:  298 SGLARRCIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKLVKENFDFRPGMITINLDLKKGGN-RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394

HSP 2 Score: 51.6026 bits (122), Expect = 9.365e-53
Identity = 26/42 (61.90%), Postives = 28/42 (66.67%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYI RQAAKSI+A+GL
Sbjct:  259 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIIASGL 300

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_PEA (S-adenosylmethionine synthetase 1 (Fragment) OS=Pisum sativum GN=SAMS1 PE=2 SV=2)

HSP 1 Score: 176.022 bits (445), Expect = 1.222e-52
Identity = 82/95 (86.32%), Postives = 90/95 (94.74%), Query Frame = 3

Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEK 404
            +G ARR IVQ+  AIGVPEPLSVFVD+YGTGKIPD+EILKIVKE+FDFRPGM++INLDL RGGNGRFLKTAAYGHFGR+DPDFTWEVVKPLKWEK
Sbjct:  271 SGLARRAIVQLLRAIGVPEPLSVFVDTYGTGKIPDREILKIVKETFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 365

HSP 2 Score: 51.6026 bits (122), Expect = 1.222e-52
Identity = 27/42 (64.29%), Postives = 28/42 (66.67%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDR GAYIVRQAAKSIVA+GL
Sbjct:  232 DTYGGWGAHGGGAFSGKDPTKVDRRGAYIVRQAAKSIVASGL 273

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_DAUCA (S-adenosylmethionine synthetase 1 OS=Daucus carota GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 1.590e-52
Identity = 91/146 (62.33%), Postives = 97/146 (66.44%), Query Frame = 3

Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAA------------------------------------------------YGHFGRDDPDFTWEVVKPLKWEKPQA 413
            +G ARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL+IVKE+FDFRPGM++INLDLKRGGNGRFLKTAA                                                YGHFGRDDPDFTWEVVKPLKWEKPQA
Sbjct:  296 SGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGTGKIPDREILKIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 441

HSP 2 Score: 53.5286 bits (127), Expect = 1.590e-52
Identity = 28/42 (66.67%), Postives = 29/42 (69.05%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVA+GL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGL 298

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK1_TRIMO (S-adenosylmethionine synthetase 1 OS=Triticum monococcum GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 2.074e-52
Identity = 83/98 (84.69%), Postives = 93/98 (94.90%), Query Frame = 3

Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            +G ARRCIVQ+SYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKE+FDFRPGM++INLDLK+GGN RF+KTAAYGHFGR+D DFTWEVVKPLK++K  A
Sbjct:  298 SGLARRCIVQISYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKKGGN-RFIKTAAYGHFGREDADFTWEVVKPLKFDKASA 394

HSP 2 Score: 51.6026 bits (122), Expect = 2.074e-52
Identity = 26/42 (61.90%), Postives = 28/42 (66.67%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYI RQAAKSI+A+GL
Sbjct:  259 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIIASGL 300

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK3_HORVU (S-adenosylmethionine synthetase 3 OS=Hordeum vulgare GN=SAM3 PE=2 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 2.704e-52
Identity = 82/98 (83.67%), Postives = 93/98 (94.90%), Query Frame = 3

Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            +G ARRCIVQ+SYAIGVPEPLSVFVDSYGTGKIPD+EILK+VKE+FDFRPGM++INLDLK+GGN RF+KTAAYGHFGRDD DFTWEVVKPLK++K  A
Sbjct:  298 SGLARRCIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGN-RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394

HSP 2 Score: 51.6026 bits (122), Expect = 2.704e-52
Identity = 26/42 (61.90%), Postives = 28/42 (66.67%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYI RQAAKSI+A+GL
Sbjct:  259 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIIASGL 300

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK_CAMSI (S-adenosylmethionine synthetase OS=Camellia sinensis GN=SAM PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 3.525e-52
Identity = 80/85 (94.12%), Postives = 82/85 (96.47%), Query Frame = 3

Query:  123 GFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 377
            G ARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKESFDFRPGM+ INLDLKRGGN RFLKTAAYGHFGRDDPDFTWE
Sbjct:  297 GLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMIAINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWE 381

HSP 2 Score: 55.4546 bits (132), Expect = 3.525e-52
Identity = 29/42 (69.05%), Postives = 29/42 (69.05%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYIVRQAAKSIVANGL
Sbjct:  257 DTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGL 298

[Close]

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Match: METK2_DIACA (S-adenosylmethionine synthetase 2 OS=Dianthus caryophyllus GN=SAM2 PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.018e-51
Identity = 81/98 (82.65%), Postives = 90/98 (91.84%), Query Frame = 3

Query:  120 TGFARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 413
            +G ARRCIVQ+SYAIGVPEPLSVFVD+YGTGKI D+EILKIVKE+FDFRPGM+ I LDLK+GGN R+LKTAAYGHFGR+DPDFTWE  K LKWEKPQA
Sbjct:  300 SGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIHDREILKIVKENFDFRPGMIAIALDLKKGGN-RYLKTAAYGHFGREDPDFTWEAAKTLKWEKPQA 396

HSP 2 Score: 51.9878 bits (123), Expect = 1.018e-51
Identity = 27/42 (64.29%), Postives = 28/42 (66.67%), Query Frame = 2

Query:    2 DTYXXXXXXXXXXXXXKDPTKVDRSGAYIVRQAAKSIVANGL 127
            DTY             KDPTKVDRSGAYI RQAAKSIVA+GL
Sbjct:  261 DTYGGWGAHGGGAFSRKDPTKVDRSGAYIARQAAKSIVASGL 302

The following BLAST results are available for this feature:

BLAST of BQ623746 vs. ExPASy Swiss-Prot

BLAST of BQ623746 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK2_ATRNU	4.228e-53	86.60	S-adenosylmethionine synthetase 2 OS=Atriplex numm...	[more]
METK1_ATRNU	4.228e-53	86.60	S-adenosylmethionine synthetase 1 OS=Atriplex numm...	[more]
METK3_PICSI	5.511e-53	86.17	S-adenosylmethionine synthetase 3 OS=Picea sitchen...	[more]
METK1_HORVU	9.365e-53	84.69	S-adenosylmethionine synthetase 1 OS=Hordeum vulga...	[more]
METK1_PEA	1.222e-52	86.32	S-adenosylmethionine synthetase 1 (Fragment) OS=Pi...	[more]
METK1_DAUCA	1.590e-52	62.33	S-adenosylmethionine synthetase 1 OS=Daucus carota...	[more]
METK1_TRIMO	2.074e-52	84.69	S-adenosylmethionine synthetase 1 OS=Triticum mono...	[more]
METK3_HORVU	2.704e-52	83.67	S-adenosylmethionine synthetase 3 OS=Hordeum vulga...	[more]
METK_CAMSI	3.525e-52	94.12	S-adenosylmethionine synthetase OS=Camellia sinens...	[more]
METK2_DIACA	1.018e-51	82.65	S-adenosylmethionine synthetase 2 OS=Dianthus cary...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_00837 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00837 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623746 ID=BQ623746; Name=BQ623746; organism=Citrus sinensis; type=EST; length=587bp
GGACACTTATGGTGGCTGGGGAGCCCATGGTGGCGGTGCTTTCTCTGGAA
AAGACCCTACTAAGGTGGATAGGAGTGGTGCATACATTGTTAGGCAGGCT
GCTAAGAGCATTGTTGCAAACGGGCTTTGCTCGCAGGTGCATTGTCCAAG
TCTCCTATGCCATTGGTGTGCCTGAGCCATTGTCTGTTTTTGTGGACTCA
TATGGAACCGGCAAGATTCCTGACAAGGAGATCCTCAAAATTGTGAAGGA
AAGCTTTGATTTCAGGCCAGGAATGATGACCATCAATTTGGATCTCAAAA
GGGGCGGTAATGGCAGGTTCTTGAAGACTGCTGCCTATGGACACTTTGGC
AGGGATGACCCAGACTTCACTTGGGAAGTTGTGAAGCCCCTCAAGTGGGA
AAAGCCTCAAGCTTAAATTTGAGTTGAAAAGTGAAAACTATCCCTGCTGT
TCAAGTTTGCTTCTATTGGTAGTGGATGAATAATTTGAATATTATCTTGC
TGCTGCTTTGTAATACTAAGCAAATCTCCTGGGTTCAAAACTCATACATG
TTCCCCATTCAATTCCCTTTCTTATTCTTCTTTCCCC