BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig809 contig Csv1_Contig809:454..857. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYC_RABIT (Serine hydroxymethyltransferase, cytosolic OS=Oryctolagus cuniculus GN=SHMT1 PE=1 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 1.986e-30
Identity = 53/94 (56.38%), Postives = 70/94 (74.47%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSET-QGTKLKDF 384
            +NHL+LV+L++K  DG R EKVLEA  IA NKNT PGD SA+ P G+R+GTPALTSRG +E+DF KVA+F    ++L V+I+ +T     LK+F
Sbjct:  355 DNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTVQIQDDTGPRATLKEF 448

HSP 2 Score: 45.4394 bits (106), Expect = 1.986e-30
Identity = 17/34 (50.00%), Postives = 27/34 (79.41%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+K YQ QV++NC   +  L+E+GY++V+GG+
Sbjct:  321 TPEFKEYQRQVVANCRALSAALVELGYKIVTGGS 354
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYC_SHEEP (Serine hydroxymethyltransferase, cytosolic OS=Ovis aries GN=SHMT1 PE=1 SV=3)

HSP 1 Score: 106.301 bits (264), Expect = 4.385e-30
Identity = 54/96 (56.25%), Postives = 72/96 (75.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTK--LKDFV 387
            +NHL+LV+L++K  DG R EKVLEA  IA NKNT PGD SA+ P G+R+GTPALTSRG +EEDF KVA+F    ++L ++I+ +  G K  LK+F+
Sbjct:  355 DNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEEDFRKVAHFIHRGIELTLQIQ-DAVGVKATLKEFM 449

HSP 2 Score: 45.4394 bits (106), Expect = 4.385e-30
Identity = 17/34 (50.00%), Postives = 27/34 (79.41%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE++AYQ QV++NC   A+ L+ +GY +V+GG+
Sbjct:  321 TPEFRAYQRQVVANCRALAEALMGLGYRVVTGGS 354
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYC_BOVIN (Serine hydroxymethyltransferase, cytosolic OS=Bos taurus GN=SHMT1 PE=2 SV=3)

HSP 1 Score: 106.301 bits (264), Expect = 4.385e-30
Identity = 54/96 (56.25%), Postives = 72/96 (75.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTK--LKDFV 387
            +NHL+LV+L++K  DG R EKVLEA  IA NKNT PGD SA+ P G+R+GTPALTSRG +EEDF KVA+F    ++L ++I+ +  G K  LK+F+
Sbjct:  355 DNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEEDFQKVAHFIHRGIELTLQIQ-DAVGVKATLKEFM 449

HSP 2 Score: 45.4394 bits (106), Expect = 4.385e-30
Identity = 17/34 (50.00%), Postives = 27/34 (79.41%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE++AYQ QV++NC   A+ L+ +GY +V+GG+
Sbjct:  321 TPEFRAYQRQVVANCRALAEALMGLGYRVVTGGS 354
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_KLULA (Serine hydroxymethyltransferase, mitochondrial OS=Kluyveromyces lactis GN=SHM1 PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.136e-29
Identity = 49/84 (58.33%), Postives = 69/84 (82.14%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSE 357
            + HL+L+NL N  IDG+R+E +LE ++IAANKNT+PGD SA+ P G+R+GTPA+T+RGF  ++FA+VA + D AVKLA+ IKS+
Sbjct:  370 DTHLILINLSNLGIDGARLETLLENINIAANKNTIPGDKSALFPSGLRVGTPAMTTRGFGPQEFAQVAAYIDRAVKLAIGIKSQ 453

HSP 2 Score: 41.5874 bits (96), Expect = 2.136e-29
Identity = 18/34 (52.94%), Postives = 24/34 (70.59%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+K YQ  V+ N   F + L++ G+ELVSGGT
Sbjct:  336 TPEFKEYQAAVVENARIFGEELVKKGFELVSGGT 369
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYC_PONAB (Serine hydroxymethyltransferase, cytosolic OS=Pongo abelii GN=SHMT1 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 2.137e-29
Identity = 50/85 (58.82%), Postives = 67/85 (78.82%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSET 360
            +NHL+LV+L++K  DG R EKVLEA  IA NKNT PGD SA+ P G+R+GTPALTSRG +E+DF KVA+F    ++L ++I+S+T
Sbjct:  355 DNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDT 439

HSP 2 Score: 42.3578 bits (98), Expect = 2.137e-29
Identity = 16/34 (47.06%), Postives = 26/34 (76.47%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+K YQ QV++NC   ++ L E+GY++V+GG+
Sbjct:  321 TLEFKVYQHQVVANCRALSEALTELGYKIVTGGS 354
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYC_HUMAN (Serine hydroxymethyltransferase, cytosolic OS=Homo sapiens GN=SHMT1 PE=1 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 2.137e-29
Identity = 50/85 (58.82%), Postives = 67/85 (78.82%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSET 360
            +NHL+LV+L++K  DG R EKVLEA  IA NKNT PGD SA+ P G+R+GTPALTSRG +E+DF KVA+F    ++L ++I+S+T
Sbjct:  355 DNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDT 439

HSP 2 Score: 42.3578 bits (98), Expect = 2.137e-29
Identity = 16/34 (47.06%), Postives = 26/34 (76.47%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+K YQ QV++NC   ++ L E+GY++V+GG+
Sbjct:  321 TLEFKVYQHQVVANCRALSEALTELGYKIVTGGS 354
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYC_MOUSE (Serine hydroxymethyltransferase, cytosolic OS=Mus musculus GN=Shmt1 PE=1 SV=2)

HSP 1 Score: 106.301 bits (264), Expect = 4.716e-29
Identity = 54/95 (56.84%), Postives = 72/95 (75.79%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSE--TQGTKLKDF 384
            +NHL+L++L++K  DG R EKVLEA  IA NKNT PGD SA+ P G+R+GTPALTSRG +EEDF KVA+F    ++L ++I+S   T+ T LK+F
Sbjct:  349 DNHLILMDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEEDFQKVAHFIHRGIELTLQIQSHMATKAT-LKEF 442

HSP 2 Score: 41.9726 bits (97), Expect = 4.716e-29
Identity = 17/34 (50.00%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+K YQ QVL+NC   +  L E+GY++V+GG+
Sbjct:  315 TTEFKIYQLQVLANCRALSDALTELGYKIVTGGS 348
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYC_CANAL (Serine hydroxymethyltransferase, cytosolic OS=Candida albicans GN=SHM2 PE=1 SV=3)

HSP 1 Score: 108.227 bits (269), Expect = 4.717e-29
Identity = 51/95 (53.68%), Postives = 73/95 (76.84%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSE--TQGTKLKDF 384
            ++H+VLV+LK+K+IDG+RVE V E ++IA NKN++PGD SA+VPGG+R+G PA+T+RG  EEDF K+  + D AV  A +++S+      KLKDF
Sbjct:  347 DSHMVLVSLKDKQIDGARVETVCEKINIALNKNSIPGDKSALVPGGVRIGAPAMTTRGLGEEDFKKIVSYIDFAVNYAKEVQSQLPKDANKLKDF 441

HSP 2 Score: 40.0466 bits (92), Expect = 4.717e-29
Identity = 18/34 (52.94%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+K YQEQVL N         + GY+LVS GT
Sbjct:  313 TPEFKEYQEQVLKNAKALESEFTKKGYKLVSDGT 346
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_HUMAN (Serine hydroxymethyltransferase, mitochondrial OS=Homo sapiens GN=SHMT2 PE=1 SV=3)

HSP 1 Score: 107.457 bits (267), Expect = 6.135e-29
Identity = 53/93 (56.99%), Postives = 70/93 (75.27%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDF 384
            +NHLVLV+L+ K +DG+R E+VLE V I ANKNT PGD SA+ PGG+R+G PALTSR F E+DF +V  F D  V + +++KS+T   KL+DF
Sbjct:  378 DNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKT--AKLQDF 468

HSP 2 Score: 40.4318 bits (93), Expect = 6.135e-29
Identity = 19/34 (55.88%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TP ++ Y  QVL N    A  LLE GY LVSGGT
Sbjct:  344 TPMFREYSLQVLKNARAMADALLERGYSLVSGGT 377
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYM_RABIT (Serine hydroxymethyltransferase, mitochondrial OS=Oryctolagus cuniculus GN=SHMT2 PE=1 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 2.295e-28
Identity = 52/93 (55.91%), Postives = 69/93 (74.19%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDF 384
            +NHLVLV+L+ K +DG+R E+VLE V I ANKNT PGD SA+ PGG+R+G PALTSR F E+DF +V  F D  V + +++K +T   KL+DF
Sbjct:  378 DNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKRKT--AKLQDF 468

HSP 2 Score: 40.4318 bits (93), Expect = 2.295e-28
Identity = 19/34 (55.88%), Postives = 21/34 (61.76%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TP ++ Y  QVL N    A  LLE GY LVSGGT
Sbjct:  344 TPMFREYSLQVLKNARAMADALLERGYSLVSGGT 377
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYC_RABIT1.986e-3056.38Serine hydroxymethyltransferase, cytosolic OS=Oryc... [more]
GLYC_SHEEP4.385e-3056.25Serine hydroxymethyltransferase, cytosolic OS=Ovis... [more]
GLYC_BOVIN4.385e-3056.25Serine hydroxymethyltransferase, cytosolic OS=Bos ... [more]
GLYM_KLULA2.136e-2958.33Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYC_PONAB2.137e-2958.82Serine hydroxymethyltransferase, cytosolic OS=Pong... [more]
GLYC_HUMAN2.137e-2958.82Serine hydroxymethyltransferase, cytosolic OS=Homo... [more]
GLYC_MOUSE4.716e-2956.84Serine hydroxymethyltransferase, cytosolic OS=Mus ... [more]
GLYC_CANAL4.717e-2953.68Serine hydroxymethyltransferase, cytosolic OS=Cand... [more]
GLYM_HUMAN6.135e-2956.99Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_RABIT2.295e-2855.91Serine hydroxymethyltransferase, mitochondrial OS=... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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