BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_RALEH (Serine hydroxymethyltransferase OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=glyA PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.982e-16
Identity = 30/64 (46.88%), Postives = 43/64 (67.19%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEED 294
            E+H++LV+L+ K I G   E++L   HI  NKN +P D     V  GIR+G+PA+T+RGF EE+
Sbjct:  313 ESHVMLVDLRAKNITGKEAERILGEAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEEE 376

HSP 2 Score: 40.0466 bits (92), Expect = 2.982e-16
Identity = 17/34 (50.00%), Postives = 24/34 (70.59%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+K YQ+QV+ N +  A+TL+  G  +VSG T
Sbjct:  279 TPEFKEYQQQVVKNAAVLAETLIARGLRIVSGRT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_GEOLS (Serine hydroxymethyltransferase OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 2.982e-16
Identity = 34/76 (44.74%), Postives = 43/76 (56.58%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAV 330
            +NHL+L+N     I G   E+ L+   I  NKNTVP +  S  V  GIR+GTPA TS G  E +  +VA F   AV
Sbjct:  309 DNHLMLINFTGTEITGKAAEEALDKAGITVNKNTVPFETRSPFVTSGIRVGTPACTSHGLKETEMEQVAGFIADAV 384

HSP 2 Score: 42.743 bits (99), Expect = 2.982e-16
Identity = 16/33 (48.48%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ+Q++ N +K A+ L+  G++L SGGT
Sbjct:  276 PEFKTYQQQIVKNAAKLAECLMAKGFKLTSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBA (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Alaska E43 / Type E3) GN=glyA PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.983e-16
Identity = 34/69 (49.28%), Postives = 47/69 (68.12%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L NK I G   EK+L+ V I  NKNTVP +  S  V  G+R+GT A+T+RGF  +D  ++A
Sbjct:  311 DNHLILVDLNNKDITGKDAEKLLDEVGITLNKNTVPNETRSPFVTSGVRIGTAAITTRGFERKDMEEIA 379

HSP 2 Score: 35.8094 bits (81), Expect = 2.983e-16
Identity = 13/33 (39.39%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K Y  +V+ NC +  + L++ G++LVS GT
Sbjct:  278 PSFKEYMARVVENCKELGEQLVKRGFKLVSNGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_RICAH (Serine hydroxymethyltransferase OS=Rickettsia akari (strain Hartford) GN=glyA PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 3.875e-16
Identity = 31/70 (44.29%), Postives = 42/70 (60.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAY 312
            +NH+VLV+L+   I G      L+   I  NKN +P D  S  +  GIR+GTPA T+RGF E+DF  V +
Sbjct:  314 DNHIVLVDLRKNGITGKLAANSLDNAGITCNKNAIPFDETSPFITSGIRLGTPACTTRGFKEQDFVSVGH 383

HSP 2 Score: 41.5874 bits (96), Expect = 3.875e-16
Identity = 16/33 (48.48%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PEYK+Y +Q++SN    A +L E GY++++GGT
Sbjct:  281 PEYKSYIKQIISNAKALASSLQERGYDILTGGT 313
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BUCBP (Serine hydroxymethyltransferase OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=glyA PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 3.876e-16
Identity = 31/68 (45.59%), Postives = 47/68 (69.12%), Query Frame = 1 
Query:  109 NHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            NHL+L++L NK I G R + +L + +I  NKN++P D +S  +  GIR+GTPA+T RGF E +  +V+
Sbjct:  316 NHLLLLDLSNKNITGKRADILLHSANIIVNKNSIPNDLLSPFITSGIRIGTPAITRRGFTELESRQVS 383

HSP 2 Score: 38.891 bits (89), Expect = 3.876e-16
Identity = 16/33 (48.48%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ QV+ N  + A+T +  GY++VSG T
Sbjct:  282 PEFKDYQYQVIKNAQEMAKTFISRGYKVVSGKT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_RHOFD (Serine hydroxymethyltransferase OS=Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) GN=glyA PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 3.876e-16
Identity = 30/64 (46.88%), Postives = 45/64 (70.31%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEED 294
            E+H++LV+L++K I G   E VL + H+  NKN +P D    MV  G+R+GTPA+T+RGF +E+
Sbjct:  312 ESHVMLVDLRSKSITGKEAEAVLGSAHMTINKNAIPNDPEKPMVTSGVRIGTPAMTTRGFKDEE 375

HSP 2 Score: 38.891 bits (89), Expect = 3.876e-16
Identity = 17/33 (51.52%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ+QVL+N    A+TL+  G  +VSG T
Sbjct:  279 PEFKVYQQQVLTNARVVAETLVSRGLRIVSGRT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_VEREI (Serine hydroxymethyltransferase OS=Verminephrobacter eiseniae (strain EF01-2) GN=glyA PE=3 SV=2)

HSP 1 Score: 65.4698 bits (158), Expect = 3.876e-16
Identity = 32/69 (46.38%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ++H++LV+L+ K I G   E VL A H+  NKN +P D    MV  G+R+GTPA+T+RGF EE     A
Sbjct:  312 QSHMMLVDLRAKGITGKEAEAVLGAAHMTINKNAIPNDPEKPMVTSGVRIGTPAMTTRGFKEEQARSTA 380

HSP 2 Score: 39.2762 bits (90), Expect = 3.876e-16
Identity = 18/33 (54.55%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P ++ YQEQVL N    AQTL   G  +VSGGT
Sbjct:  279 PGFRLYQEQVLKNAKVLAQTLAARGLRIVSGGT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBB (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Eklund 17B / Type B) GN=glyA PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.877e-16
Identity = 34/69 (49.28%), Postives = 46/69 (66.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L NK I G   EK+L+ V I  NKNTVP +  S  V  G+R+GT A+T+RGF   D  ++A
Sbjct:  311 DNHLILVDLNNKDITGKDAEKLLDEVGITLNKNTVPNETRSPFVTSGVRIGTAAITTRGFERRDMEEIA 379

HSP 2 Score: 35.8094 bits (81), Expect = 3.877e-16
Identity = 13/33 (39.39%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K Y  +V+ NC +  + L++ G++LVS GT
Sbjct:  278 PSFKEYMARVVENCKELGEQLVKRGFKLVSNGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CHLTE (Serine hydroxymethyltransferase OS=Chlorobium tepidum GN=glyA PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 5.030e-16
Identity = 33/79 (41.77%), Postives = 47/79 (59.49%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVP-GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLA 339
            +NHL+L++L+NK ++G   E +L    I  NKN VP  D S  V  GIR+GTPA+T+RG    +  K+  F D  +  A
Sbjct:  330 KNHLMLLDLRNKNVNGKVAENLLHEAGITVNKNMVPFDDKSPFVTSGIRIGTPAMTTRGMKVAEAEKIVEFIDRVISAA 408

HSP 2 Score: 38.5058 bits (88), Expect = 5.030e-16
Identity = 16/33 (48.48%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+KAY +Q+  N +  A   L  GY +VSGGT
Sbjct:  297 PEFKAYAQQIKDNAAAMAAKFLAAGYHIVSGGT 329
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ROSS1 (Serine hydroxymethyltransferase OS=Roseiflexus sp. (strain RS-1) GN=glyA PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.032e-16
Identity = 31/76 (40.79%), Postives = 48/76 (63.16%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPG-DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAV 330
            +NHL+L++L+NK + G   ++ L+   I  NKN VP  D S ++  GIR+GTPALT+RG  E +  ++A   D  +
Sbjct:  329 DNHLMLIDLRNKAVSGKAAQEALDRAAITTNKNAVPNDDKSPLITSGIRLGTPALTTRGMKEPEMEQIAALIDDVI 404

HSP 2 Score: 35.8094 bits (81), Expect = 5.032e-16
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE++ Y  QV+ N    A  L+  GY ++SGGT
Sbjct:  296 PEFETYARQVIRNAQTLANALIARGYHVLSGGT 328
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_RALEH2.982e-1646.88Serine hydroxymethyltransferase OS=Ralstonia eutro... [more]
GLYA_GEOLS2.982e-1644.74Serine hydroxymethyltransferase OS=Geobacter lovle... [more]
GLYA_CLOBA2.983e-1649.28Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_RICAH3.875e-1644.29Serine hydroxymethyltransferase OS=Rickettsia akar... [more]
GLYA_BUCBP3.876e-1645.59Serine hydroxymethyltransferase OS=Buchnera aphidi... [more]
GLYA_RHOFD3.876e-1646.88Serine hydroxymethyltransferase OS=Rhodoferax ferr... [more]
GLYA_VEREI3.876e-1646.38Serine hydroxymethyltransferase OS=Verminephrobact... [more]
GLYA_CLOBB3.877e-1649.28Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_CHLTE5.030e-1641.77Serine hydroxymethyltransferase OS=Chlorobium tepi... [more]
GLYA_ROSS15.032e-1640.79Serine hydroxymethyltransferase OS=Roseiflexus sp.... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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