BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LEPCP (Serine hydroxymethyltransferase OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.106e-15
Identity = 31/63 (49.21%), Postives = 42/63 (66.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEE 291
            E+H++LV+L+ K I G   E +L   HI  NKN +P D    MV  GIR+G+PA+T+RGF EE
Sbjct:  313 ESHVMLVDLRPKGITGKEAEALLGRAHITCNKNGIPNDPQKPMVTSGIRLGSPAMTTRGFKEE 375

HSP 2 Score: 40.4318 bits (93), Expect = 1.106e-15
Identity = 18/33 (54.55%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+KAYQEQV+ N +  A+TL   G  +VSG T
Sbjct:  280 PEFKAYQEQVVKNATAMAETLTARGLRIVSGRT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ALTMD (Serine hydroxymethyltransferase OS=Alteromonas macleodii (strain DSM 17117 / Deep ecotype) GN=glyA PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.437e-15
Identity = 32/69 (46.38%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL L++L +K I G   +  L A +I  NKN+VP D  S  V  G+R+G+PA+T RGF EE   +VA
Sbjct:  316 DNHLFLLDLIDKDITGKDADAALGAANITVNKNSVPNDPRSPFVTSGLRIGSPAITRRGFKEEQAKQVA 384

HSP 2 Score: 42.3578 bits (98), Expect = 1.437e-15
Identity = 17/33 (51.52%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+KAYQ+QV++N       + E GY +VSGGT
Sbjct:  283 PEFKAYQQQVVANAKAMVSVMQERGYNIVSGGT 315
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_GEOMG (Serine hydroxymethyltransferase OS=Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) GN=glyA PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.437e-15
Identity = 33/76 (43.42%), Postives = 43/76 (56.58%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAV 330
            +NHL+LVNL    + G   E+ L+   I  NKNTVP +  S  V  G R+GTPA TS G  E +  +VA F   A+
Sbjct:  309 DNHLMLVNLTGTELTGKVAEEALDKAGITVNKNTVPFETRSPFVTSGFRIGTPAATSHGLKEAEMVEVAAFIAEAL 384

HSP 2 Score: 41.5874 bits (96), Expect = 1.437e-15
Identity = 17/33 (51.52%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQEQ++ N    A  L++ G+ LVSGGT
Sbjct:  276 PEFKLYQEQIVKNARTLADELMKRGFRLVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CAMHC (Serine hydroxymethyltransferase OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=glyA PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.437e-15
Identity = 32/69 (46.38%), Postives = 41/69 (59.42%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+   K   G      LE   I  NKNTVPG+  S  V  GIR+G+ ALTSRG  E++F  +A
Sbjct:  309 DNHLILVSFLKKDYSGKDASNALENAGITVNKNTVPGETRSPFVTSGIRVGSAALTSRGMKEKEFEWIA 377

HSP 2 Score: 42.3578 bits (98), Expect = 1.437e-15
Identity = 17/34 (50.00%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +PE+K Y +QV +NC     TL++ G++LVSGGT
Sbjct:  275 SPEWKTYAKQVKANCKILGDTLMKRGFDLVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SYNWW (Serine hydroxymethyltransferase OS=Syntrophomonas wolfei subsp. wolfei (strain Goettingen) GN=glyA PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.437e-15
Identity = 29/69 (42.03%), Postives = 46/69 (66.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+++ K ++G   E +LE+++I  NKN +P D     V  GIR+GTPA+TSR    +D  ++A
Sbjct:  313 DNHLMLVDVRPKGLNGRDAEAILESINITVNKNAIPFDPEKPTVTSGIRVGTPAVTSRALKGDDMRELA 381

HSP 2 Score: 42.743 bits (99), Expect = 1.437e-15
Identity = 17/34 (50.00%), Postives = 26/34 (76.47%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+KAYQ+ +++N +  A+ L+E G  +VSGGT
Sbjct:  279 TPEFKAYQQDIVNNAAILAKALMEQGLRVVSGGT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOCE (Serine hydroxymethyltransferase OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) GN=glyA PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.438e-15
Identity = 33/69 (47.83%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+L+NL N  I G   +  L+ V I  NKN +P D  S  +  GIR+GTPA+TSRG  EED  ++A
Sbjct:  312 DNHLMLINLTNMNITGKEAQHKLDEVCITCNKNGIPFDTQSPFITSGIRLGTPAVTSRGMNEEDMKEIA 380

HSP 2 Score: 36.1946 bits (82), Expect = 1.438e-15
Identity = 14/34 (41.18%), Postives = 22/34 (64.71%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T ++K YQ+ ++ N    A  L+E G++LVS GT
Sbjct:  278 TDDFKIYQQNIVKNAKALASALMEKGFKLVSDGT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_PROVI (Serine hydroxymethyltransferase OS=Prosthecochloris vibrioformis (strain DSM 265) (strain DSM 265) GN=glyA PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.865e-15
Identity = 37/93 (39.78%), Postives = 52/93 (55.91%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVP-GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKD 381
            +NHL+L++L+NK I G  VE  L    I  NKN VP  D S  V  GIR+GT A+T+RG  E+D   +A   D  +  A   +S +    +K+
Sbjct:  330 KNHLMLLDLRNKDITGKVVENTLHEAGITVNKNMVPFDDKSPFVTSGIRIGTAAMTTRGMNEDDSRLIAELIDRVILSAASPESSSVCRSVKE 422

HSP 2 Score: 37.3502 bits (85), Expect = 1.865e-15
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE++ Y  QV SN +  ++  L +GY +VSGGT
Sbjct:  297 PEFRQYAMQVRSNAAAMSERFLSLGYNIVSGGT 329
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CYAP8 (Serine hydroxymethyltransferase OS=Cyanothece sp. (strain PCC 8801) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.867e-15
Identity = 29/68 (42.65%), Postives = 48/68 (70.59%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHL+LV+L++  + G   ++++  ++I ANKNTVP D  S  V  G+R+G+PALT+RG  E +F ++
Sbjct:  315 DNHLMLVDLRSIGMTGKEADRLVSEINITANKNTVPFDPESPFVTSGLRLGSPALTTRGMGESEFKEI 382

HSP 2 Score: 40.0466 bits (92), Expect = 1.867e-15
Identity = 17/33 (51.52%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P++KAY  QV++N    A  L+E G++LVSGGT
Sbjct:  282 PQFKAYSGQVIANSRALAAQLMERGFKLVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_THEM4 (Serine hydroxymethyltransferase OS=Thermosipho melanesiensis (strain BI429 / DSM 12029) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.867e-15
Identity = 33/85 (38.82%), Postives = 48/85 (56.47%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSE 357
            + HL+LV+L    + G   E  L   H+  NKNT+P +  S  V  GIR+GTPALT+RG  E +  ++A   +  VK+   +K E
Sbjct:  311 DTHLMLVDLNPLNVTGKAAEIALGKCHVTVNKNTIPNETRSPFVASGIRLGTPALTTRGMKESEMEEIA---ELIVKVLENVKDE 392

HSP 2 Score: 40.0466 bits (92), Expect = 1.867e-15
Identity = 18/34 (52.94%), Postives = 23/34 (67.65%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+KAYQ QV+ N  K A+ L + G  +VSGGT
Sbjct:  277 TDEFKAYQNQVVKNAKKLAEELEKRGLRIVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SHISS (Serine hydroxymethyltransferase OS=Shigella sonnei (strain Ss046) GN=glyA PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.868e-15
Identity = 31/69 (44.93%), Postives = 43/69 (62.32%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL LV+L +K + G   +  L   +I  NKN+VP D  S  V  GIR+GTPA+T RGF E +  ++A
Sbjct:  315 DNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELA 383

HSP 2 Score: 41.9726 bits (97), Expect = 1.868e-15
Identity = 17/33 (51.52%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ+QV  N     +  LE GY++VSGGT
Sbjct:  282 PEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGT 314
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_LEPCP1.106e-1549.21Serine hydroxymethyltransferase OS=Leptothrix chol... [more]
GLYA_ALTMD1.437e-1546.38Serine hydroxymethyltransferase OS=Alteromonas mac... [more]
GLYA_GEOMG1.437e-1543.42Serine hydroxymethyltransferase OS=Geobacter metal... [more]
GLYA_CAMHC1.437e-1546.38Serine hydroxymethyltransferase OS=Campylobacter h... [more]
GLYA_SYNWW1.437e-1542.03Serine hydroxymethyltransferase OS=Syntrophomonas ... [more]
GLYA_CLOCE1.438e-1547.83Serine hydroxymethyltransferase OS=Clostridium cel... [more]
GLYA_PROVI1.865e-1539.78Serine hydroxymethyltransferase OS=Prosthecochlori... [more]
GLYA_CYAP81.867e-1542.65Serine hydroxymethyltransferase OS=Cyanothece sp. ... [more]
GLYA_THEM41.867e-1538.82Serine hydroxymethyltransferase OS=Thermosipho mel... [more]
GLYA_SHISS1.868e-1544.93Serine hydroxymethyltransferase OS=Shigella sonnei... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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