BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYC1_DICDI (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum GN=shmt1 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.337e-20
Identity = 44/94 (46.81%), Postives = 63/94 (67.02%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFV 387
            +NHL+L +L+ K + G++ EK  +  +I  NKN V GD +A+ PGGIR+G+ ALTSRG  E DF K+A F D  V ++++I+    G KL DFV
Sbjct:  337 DNHLILWDLRPKELTGNKFEKAADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEIQGRV-GKKLVDFV 429

HSP 2 Score: 30.4166 bits (67), Expect = 2.337e-20
Identity = 13/32 (40.62%), Postives = 18/32 (56.25%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K Y  QV  N +     L+  GY+LV+ GT
Sbjct:  305 EFKEYALQVKKNAAAIGNALMNKGYKLVTNGT 336
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DESRM (Serine hydroxymethyltransferase OS=Desulfotomaculum reducens (strain MI-1) GN=glyA PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.045e-20
Identity = 42/94 (44.68%), Postives = 56/94 (59.57%), Query Frame = 1 
Query:   85 NLFLVEPENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQ 363
            NL     +NHL+LV+L+   I G + E +L+ VHI  NKN +P D     V  GIR+GTPA+T+RGF E+D  KVA     A+ L  K   +TQ
Sbjct:  304 NLVSGGTDNHLILVDLRGTGITGKQAETLLDEVHITCNKNAIPFDPEKPFVTSGIRLGTPAVTTRGFKEKDMDKVAEII--ALTLQEKDNPDTQ 395

HSP 2 Score: 45.8246 bits (107), Expect = 3.045e-20
Identity = 19/33 (57.58%), Postives = 26/33 (78.79%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+KAYQ+Q+++N    A+ LLE G+ LVSGGT
Sbjct:  278 PEFKAYQQQIINNAQALAKGLLERGFNLVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOK5 (Serine hydroxymethyltransferase OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) GN=glyA PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.046e-20
Identity = 40/77 (51.95%), Postives = 56/77 (72.73%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+LV+L NK I G  +E++L+ V+I  NKN +P D +   V  GIR+GTPA+T+RGF EE+  KVAYF + AV+
Sbjct:  311 DNHLLLVDLTNKNITGKDLEELLDKVNITVNKNAIPFDKLKPNVTSGIRVGTPAVTTRGFKEEEMKKVAYFINKAVE 387

HSP 2 Score: 37.7354 bits (86), Expect = 3.046e-20
Identity = 15/32 (46.88%), Postives = 22/32 (68.75%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            EYK Y +Q++ N   F + L++ G+ LVSGGT
Sbjct:  279 EYKEYIDQIIKNAKVFGEELVKYGFRLVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOK1 (Serine hydroxymethyltransferase OS=Clostridium kluyveri (strain NBRC 12016) GN=glyA PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.046e-20
Identity = 40/77 (51.95%), Postives = 56/77 (72.73%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+LV+L NK I G  +E++L+ V+I  NKN +P D +   V  GIR+GTPA+T+RGF EE+  KVAYF + AV+
Sbjct:  311 DNHLLLVDLTNKNITGKDLEELLDKVNITVNKNAIPFDKLKPNVTSGIRVGTPAVTTRGFKEEEMKKVAYFINKAVE 387

HSP 2 Score: 37.7354 bits (86), Expect = 3.046e-20
Identity = 15/32 (46.88%), Postives = 22/32 (68.75%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            EYK Y +Q++ N   F + L++ G+ LVSGGT
Sbjct:  279 EYKEYIDQIIKNAKVFGEELVKYGFRLVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ALKOO (Serine hydroxymethyltransferase OS=Alkaliphilus oremlandii (strain OhILAs) GN=glyA PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.474e-19
Identity = 36/76 (47.37%), Postives = 50/76 (65.79%), Query Frame = 1 
Query:   85 NLFLVEPENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            NL     +NHL+L++L+NK I G   EK+L+ V +  NKNT+P D  S  V  GIR+GTPA+T+RG  E+D   +A
Sbjct:  304 NLVSGGTDNHLLLLDLRNKNITGKDAEKLLDEVGVTVNKNTIPYDPESPFVTSGIRIGTPAVTTRGMKEDDMVTIA 379

HSP 2 Score: 45.0542 bits (105), Expect = 1.474e-19
Identity = 19/34 (55.88%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +PE+KAYQEQV+ N +K  + L   G+ LVSGGT
Sbjct:  277 SPEFKAYQEQVIKNAAKLGEELKSRGFNLVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CALS8 (Serine hydroxymethyltransferase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=glyA PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 4.216e-19
Identity = 38/69 (55.07%), Postives = 51/69 (73.91%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L+NK I G   EK L++++I  NKN +P D  S MV  GIR+GTPA+T+RGF EED  +VA
Sbjct:  314 DNHLMLVDLRNKGITGKDAEKRLDSLNITCNKNAIPFDTQSPMVTSGIRLGTPAVTTRGFKEEDMIEVA 382

HSP 2 Score: 38.5058 bits (88), Expect = 4.216e-19
Identity = 16/34 (47.06%), Postives = 23/34 (67.65%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E++ YQ Q+L N    ++ L+E G+ LVSGGT
Sbjct:  280 TEEFRNYQIQILKNAKALSERLIERGFRLVSGGT 313
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBM (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Loch Maree / Type A3) GN=glyA PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.483e-19
Identity = 37/77 (48.05%), Postives = 57/77 (74.03%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+L++L NK I G   EK+L++V I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+  K+AYF + +++
Sbjct:  311 DNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387

HSP 2 Score: 33.113 bits (74), Expect = 5.483e-19
Identity = 13/32 (40.62%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +YK Y +QV+ N     + L   G+ L+SGGT
Sbjct:  279 DYKEYMQQVVKNTKVLGEELKNYGFRLISGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBL (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=glyA PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.483e-19
Identity = 37/77 (48.05%), Postives = 57/77 (74.03%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+L++L NK I G   EK+L++V I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+  K+AYF + +++
Sbjct:  311 DNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387

HSP 2 Score: 33.113 bits (74), Expect = 5.483e-19
Identity = 13/32 (40.62%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +YK Y +QV+ N     + L   G+ L+SGGT
Sbjct:  279 DYKEYMQQVVKNTKVLGEELKNYGFRLISGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBK (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Okra / Type B1) GN=glyA PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.483e-19
Identity = 37/77 (48.05%), Postives = 57/77 (74.03%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+L++L NK I G   EK+L++V I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+  K+AYF + +++
Sbjct:  311 DNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387

HSP 2 Score: 33.113 bits (74), Expect = 5.483e-19
Identity = 13/32 (40.62%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +YK Y +QV+ N     + L   G+ L+SGGT
Sbjct:  279 DYKEYMQQVVKNTKVLGEELKNYGFRLISGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBJ (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Kyoto / Type A2) GN=glyA PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.483e-19
Identity = 37/77 (48.05%), Postives = 57/77 (74.03%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+L++L NK I G   EK+L++V I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+  K+AYF + +++
Sbjct:  311 DNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387

HSP 2 Score: 33.113 bits (74), Expect = 5.483e-19
Identity = 13/32 (40.62%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +YK Y +QV+ N     + L   G+ L+SGGT
Sbjct:  279 DYKEYMQQVVKNTKVLGEELKNYGFRLISGGT 310
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYC1_DICDI2.337e-2046.81Serine hydroxymethyltransferase 1 OS=Dictyostelium... [more]
GLYA_DESRM3.045e-2044.68Serine hydroxymethyltransferase OS=Desulfotomaculu... [more]
GLYA_CLOK53.046e-2051.95Serine hydroxymethyltransferase OS=Clostridium klu... [more]
GLYA_CLOK13.046e-2051.95Serine hydroxymethyltransferase OS=Clostridium klu... [more]
GLYA_ALKOO1.474e-1947.37Serine hydroxymethyltransferase OS=Alkaliphilus or... [more]
GLYA_CALS84.216e-1955.07Serine hydroxymethyltransferase OS=Caldicellulosir... [more]
GLYA_CLOBM5.483e-1948.05Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_CLOBL5.483e-1948.05Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_CLOBK5.483e-1948.05Serine hydroxymethyltransferase OS=Clostridium bot... [more]
GLYA_CLOBJ5.483e-1948.05Serine hydroxymethyltransferase OS=Clostridium bot... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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