BQ623771 | Citrus Genome Database

Overview


Name	BQ623771
Unique Name	BQ623771
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	405

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig809	contig	Csv1_Contig809:454..857.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBH (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) GN=glyA PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.483e-19
Identity = 37/77 (48.05%), Postives = 57/77 (74.03%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+L++L NK I G   EK+L++V I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+  K+AYF + +++
Sbjct:  311 DNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387

HSP 2 Score: 33.113 bits (74), Expect = 5.483e-19
Identity = 13/32 (40.62%), Postives = 19/32 (59.38%), Query Frame = 3

Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +YK Y +QV+ N     + L   G+ L+SGGT
Sbjct:  279 DYKEYMQQVVKNTKVLGEELKNYGFRLISGGT 310

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOB6 (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain 657 / Type Ba4) GN=glyA PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.483e-19
Identity = 37/77 (48.05%), Postives = 57/77 (74.03%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+L++L NK I G   EK+L++V I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+  K+AYF + +++
Sbjct:  311 DNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387

HSP 2 Score: 33.113 bits (74), Expect = 5.483e-19
Identity = 13/32 (40.62%), Postives = 19/32 (59.38%), Query Frame = 3

Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +YK Y +QV+ N     + L   G+ L+SGGT
Sbjct:  279 DYKEYMQQVVKNTKVLGEELKNYGFRLISGGT 310

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOB1 (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain ATCC 19397 / Type A) GN=glyA PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.483e-19
Identity = 37/77 (48.05%), Postives = 57/77 (74.03%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+L++L NK I G   EK+L++V I  NKNT+P + +S  +  GIR+GTPA+T+RGF EE+  K+AYF + +++
Sbjct:  311 DNHLLLIDLTNKNITGKDAEKLLDSVGITVNKNTIPFETLSPFITSGIRIGTPAVTTRGFKEEEMKKIAYFMNYSIE 387

HSP 2 Score: 33.113 bits (74), Expect = 5.483e-19
Identity = 13/32 (40.62%), Postives = 19/32 (59.38%), Query Frame = 3

Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +YK Y +QV+ N     + L   G+ L+SGGT
Sbjct:  279 DYKEYMQQVVKNTKVLGEELKNYGFRLISGGT 310

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_NATTJ (Serine hydroxymethyltransferase OS=Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) GN=glyA PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 5.484e-19
Identity = 35/76 (46.05%), Postives = 51/76 (67.11%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSA-MVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAV 330
            +NHL+LV+L+ K + G + E+VL+ VHI  NKN VP D     V  G+R+GTPA+T+RGF E++  +VA   D  +
Sbjct:  309 DNHLMLVDLQKKGVTGKKAERVLDDVHITVNKNAVPNDPEGPFVTSGLRLGTPAVTTRGFAEDEIKEVAQLLDKVI 384

HSP 2 Score: 37.7354 bits (86), Expect = 5.484e-19
Identity = 16/32 (50.00%), Postives = 23/32 (71.88%), Query Frame = 3

Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K YQ+QV+ N S  A  L  +GY+LV+GG+
Sbjct:  277 EFKNYQKQVIKNASVLADELNNLGYDLVAGGS 308

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DESDG (Serine hydroxymethyltransferase OS=Desulfovibrio desulfuricans (strain G20) GN=glyA PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 7.132e-19
Identity = 36/69 (52.17%), Postives = 45/69 (65.22%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L NK I G   +  L+   I ANKNTVP +  S  V  GIR+GTPALT+RG  E +  KVA
Sbjct:  309 DNHLMLVDLTNKDITGKDAQHALDLAGITANKNTVPFETRSPFVTSGIRLGTPALTTRGMKEAEMVKVA 377

HSP 2 Score: 46.2098 bits (108), Expect = 7.132e-19
Identity = 20/33 (60.61%), Postives = 27/33 (81.82%), Query Frame = 3

Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+ AYQ+QV++N +K A TL + G+ELVSGGT
Sbjct:  276 PEFGAYQKQVVANAAKLAATLTDAGFELVSGGT 308

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_PHYAS (Serine hydroxymethyltransferase OS=Phytoplasma australiense GN=glyA PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.206e-18
Identity = 38/79 (48.10%), Postives = 51/79 (64.56%), Query Frame = 1

Query:  106 ENHLVLVNLK--NKRIDGSRVEKVLEAVHIAANKNTVPGDVSA-MVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL L+NLK  N    G ++  +LE V+I  NKNT+P D    M   GIR+GTPA+T++GF E DF K+A   D A+K
Sbjct:  312 DNHLFLINLKKTNPLFTGEKIANILEKVNIIVNKNTIPFDQEKPMFTSGIRLGTPAMTTKGFQEADFIKLADLIDQAIK 390

HSP 2 Score: 39.2762 bits (90), Expect = 1.206e-18
Identity = 17/32 (53.12%), Postives = 22/32 (68.75%), Query Frame = 3

Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K YQ+QV+ N   FAQT  + GY +VS GT
Sbjct:  280 EFKNYQQQVIKNAQAFAQTFQKKGYHVVSQGT 311

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOB8 (Serine hydroxymethyltransferase OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) GN=glyA PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.206e-18
Identity = 36/77 (46.75%), Postives = 52/77 (67.53%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NH+ LV+L NK I G   E +L++V I  NKNTVP +  S  V  GIR+GT A+T+RGFV++D A++A     A++
Sbjct:  311 DNHVFLVDLNNKDITGKEAEALLDSVGITVNKNTVPNETRSPFVTSGIRIGTAAITTRGFVKDDMAEIAAVISEAIE 387

HSP 2 Score: 40.0466 bits (92), Expect = 1.206e-18
Identity = 15/33 (45.45%), Postives = 23/33 (69.70%), Query Frame = 3

Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K Y E V+ NC + A+ L+  G+++VSGGT
Sbjct:  278 PSFKTYGENVVENCKELAEQLIARGFKIVSGGT 310

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ALHEH (Serine hydroxymethyltransferase OS=Alkalilimnicola ehrlichei (strain MLHE-1) GN=glyA PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.038e-18
Identity = 32/69 (46.38%), Postives = 45/69 (65.22%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL L++L +K + G   +  L   HI  NKNTVP D  S  V  G+R+GTPA+T+RGF EE+  ++A
Sbjct:  315 DNHLFLIDLVDKGLTGKAADAALGKAHITVNKNTVPNDPQSPFVTSGLRIGTPAITTRGFKEEECRELA 383

HSP 2 Score: 46.9802 bits (110), Expect = 2.038e-18
Identity = 20/33 (60.61%), Postives = 27/33 (81.82%), Query Frame = 3

Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+KAYQ+QV++N    AQ L+E GY++VSGGT
Sbjct:  282 PEFKAYQQQVVANARAMAQGLIERGYKVVSGGT 314

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ANATD (Serine hydroxymethyltransferase OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=glyA PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 2.039e-18
Identity = 37/69 (53.62%), Postives = 49/69 (71.01%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L+NK I G   EK+L+  +I  NKN VP D  S M+  GIR+GTPA+T+RGF E D  +VA
Sbjct:  312 DNHLMLVDLRNKGITGKDAEKILDEHNITCNKNAVPFDTQSPMITSGIRLGTPAVTTRGFKEGDMLEVA 380

HSP 2 Score: 38.891 bits (89), Expect = 2.039e-18
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 3

Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+K YQ Q+L N    +  L+E G+ LVSGGT
Sbjct:  278 TEEFKNYQVQILKNAKALSTRLIERGFRLVSGGT 311

[Close]

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_PELTS (Serine hydroxymethyltransferase OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) GN=glyA PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.039e-18
Identity = 33/77 (42.86%), Postives = 52/77 (67.53%), Query Frame = 1

Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVS-AMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+LV+L++K+I G   +++ +AV +  NKN VP D     +  GIR+GTPA+TSRG  E+D  ++A   D A++
Sbjct:  314 DNHLILVDLRSKKITGREAQELFDAVGVTVNKNAVPFDPQPPNIASGIRIGTPAVTSRGLNEDDMVQIAEIMDYAIE 390

HSP 2 Score: 41.5874 bits (96), Expect = 2.039e-18
Identity = 18/33 (54.55%), Postives = 23/33 (69.70%), Query Frame = 3

Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ +++SN    A  LLE G+ELVSGGT
Sbjct:  281 PGFKEYQRKIVSNARALADALLERGFELVSGGT 313

The following BLAST results are available for this feature:

BLAST of BQ623771 vs. ExPASy Swiss-Prot

BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
GLYA_CLOBH	5.483e-19	48.05	Serine hydroxymethyltransferase OS=Clostridium bot...	[more]
GLYA_CLOB6	5.483e-19	48.05	Serine hydroxymethyltransferase OS=Clostridium bot...	[more]
GLYA_CLOB1	5.483e-19	48.05	Serine hydroxymethyltransferase OS=Clostridium bot...	[more]
GLYA_NATTJ	5.484e-19	46.05	Serine hydroxymethyltransferase OS=Natranaerobius ...	[more]
GLYA_DESDG	7.132e-19	52.17	Serine hydroxymethyltransferase OS=Desulfovibrio d...	[more]
GLYA_PHYAS	1.206e-18	48.10	Serine hydroxymethyltransferase OS=Phytoplasma aus...	[more]
GLYA_CLOB8	1.206e-18	46.75	Serine hydroxymethyltransferase OS=Clostridium bei...	[more]
GLYA_ALHEH	2.038e-18	46.38	Serine hydroxymethyltransferase OS=Alkalilimnicola...	[more]
GLYA_ANATD	2.039e-18	53.62	Serine hydroxymethyltransferase OS=Anaerocellum th...	[more]
GLYA_PELTS	2.039e-18	42.86	Serine hydroxymethyltransferase OS=Pelotomaculum t...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT