BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ANAVT (Serine hydroxymethyltransferase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=glyA PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.599e-13
Identity = 30/68 (44.12%), Postives = 49/68 (72.06%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHL+LV+L++  + G R ++++  V+I ANKNTVP D  S  V  G+R+G+PA+T+RG  E +F ++
Sbjct:  315 DNHLMLVDLRSVNMTGKRADQLVSEVNITANKNTVPFDPQSPFVTSGLRLGSPAMTTRGMGEAEFTEI 382

HSP 2 Score: 31.187 bits (69), Expect = 1.599e-13
Identity = 14/33 (42.42%), Postives = 18/33 (54.55%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE++ Y  QV+ N    A  L   G +LVS GT
Sbjct:  282 PEFQGYSAQVIENARALANQLQNRGLKLVSDGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ACIC1 (Serine hydroxymethyltransferase OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) GN=glyA PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.599e-13
Identity = 32/81 (39.51%), Postives = 45/81 (55.56%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVK 345
            +NHL+LV+L +K + G  V + L+   I  N NTVP D      P GIR+GTPA+TSRG    +  ++A + D       K
Sbjct:  316 DNHLILVDLTSKGVAGKPVARALDRAGIELNYNTVPFDPRKPFDPSGIRLGTPAVTSRGMGPAEMRQIAAWIDEVTTAVAK 396

HSP 2 Score: 36.1946 bits (82), Expect = 1.599e-13
Identity = 14/33 (42.42%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE++ Y   +++N +  A+ LL  G++LVSGGT
Sbjct:  283 PEFRDYARNIVANAAVLAEELLARGFDLVSGGT 315
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA1_PSESM (Serine hydroxymethyltransferase 1 OS=Pseudomonas syringae pv. tomato GN=glyA1 PE=3 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 1.600e-13
Identity = 28/60 (46.67%), Postives = 38/60 (63.33%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGF 282
            +NHL LV+L  + + G   +  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RGF
Sbjct:  315 DNHLFLVSLIRQGLTGKEADAALGRAHITVNKNSVPNDPQSPFVTSGLRIGTPAVTTRGF 374

HSP 2 Score: 38.891 bits (89), Expect = 1.600e-13
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ+QV+ N    AQ  +  G+++VSGGT
Sbjct:  282 PGFKTYQQQVIDNAQAMAQVFITRGFDVVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA2_PSEF5 (Serine hydroxymethyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA2 PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.600e-13
Identity = 28/60 (46.67%), Postives = 37/60 (61.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGF 282
            +NHL LV+L  + + G   +  L   HI  NKN VP D  S  V  G+R+GTPA+T+RGF
Sbjct:  315 DNHLFLVSLIRQGLTGKDADAALGRAHITVNKNAVPNDPQSPFVTSGLRIGTPAVTTRGF 374

HSP 2 Score: 40.0466 bits (92), Expect = 1.600e-13
Identity = 16/33 (48.48%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +KAYQ+QV+ N    A   ++ GY++VSGGT
Sbjct:  282 PGFKAYQQQVIDNAQAMAGVFIKRGYDVVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_HALMA (Serine hydroxymethyltransferase OS=Haloarcula marismortui GN=glyA PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.600e-13
Identity = 37/71 (52.11%), Postives = 46/71 (64.79%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRID--GSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHLVL++L+    D  G  VE+ LE   I  N NTVPG+  SA  P GIR GTPALT+RGF E+   +VA
Sbjct:  311 DNHLVLIDLRPSHPDTTGKEVEEALEEAGIVLNANTVPGETRSAFNPSGIRAGTPALTTRGFDEDACREVA 381

HSP 2 Score: 30.8018 bits (68), Expect = 1.600e-13
Identity = 13/33 (39.39%), Postives = 18/33 (54.55%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE++ Y +Q + N     +   E G  LVSGGT
Sbjct:  278 PEFEQYAQQTVDNAIALGEQFKEHGLSLVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BACWK (Serine hydroxymethyltransferase OS=Bacillus weihenstephanensis (strain KBAB4) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.600e-13
Identity = 31/69 (44.93%), Postives = 46/69 (66.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+L++++N  I G   E VL+ V I  NKNT+P +  S  V  G+R+GT A+TSRGF  E+  ++A
Sbjct:  309 DNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEEMDEIA 377

HSP 2 Score: 33.113 bits (74), Expect = 1.600e-13
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K Y + +++N ++ A+ L + G  LVSGGT
Sbjct:  277 EFKTYAQHIINNANRLAEGLQKEGLTLVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA1_RHILO (Serine hydroxymethyltransferase 1 OS=Rhizobium loti GN=glyA1 PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.076e-13
Identity = 34/88 (38.64%), Postives = 50/88 (56.82%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQG 366
            +NHL+LV+L+ K   G R E  L   +I  NKN +P D     V  G+R+GTPA T+RGF + +F ++     A V   +KI +  +G
Sbjct:  325 DNHLMLVDLRPKNATGKRAEAALGRANITCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFGQAEFREIGKLI-AEVLDGLKIANSDEG 411

HSP 2 Score: 35.4242 bits (80), Expect = 2.076e-13
Identity = 15/33 (45.45%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K Y E V +N    A +L E G ++VSGGT
Sbjct:  292 PSFKVYAESVAANAKALASSLKETGLDIVSGGT 324
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA2_VIBVY (Serine hydroxymethyltransferase 2 OS=Vibrio vulnificus (strain YJ016) GN=glyA2 PE=3 SV=2)

HSP 1 Score: 62.7734 bits (151), Expect = 2.076e-13
Identity = 30/71 (42.25%), Postives = 46/71 (64.79%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            + HL+LV+L+ K + G++ E+ LE   I  NKN +P D    M+  G+R+GTPA TSRGF  E+F  + ++
Sbjct:  324 DTHLMLVDLRPKGLKGNKAEEALERAGITCNKNGIPFDTEKPMITSGVRLGTPAGTSRGFGAEEFKLIGHW 394

HSP 2 Score: 32.7278 bits (73), Expect = 2.076e-13
Identity = 13/33 (39.39%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V++N    A+ L   G ++V+GGT
Sbjct:  291 PEFKTYIDSVINNAKVLAEVLQTRGCDIVTGGT 323
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SYNP6 (Serine hydroxymethyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=glyA PE=3 SV=2)

HSP 1 Score: 62.7734 bits (151), Expect = 2.077e-13
Identity = 34/69 (49.28%), Postives = 48/69 (69.57%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L++  + G   + ++  V+I ANKNTVP D  S  V  GIR+GT A+T+RGF E +FA VA
Sbjct:  315 DNHLLLVDLRSIGMTGKVADLLVSDVNITANKNTVPFDPESPFVTSGIRLGTAAMTTRGFKEAEFAIVA 383

HSP 2 Score: 32.7278 bits (73), Expect = 2.077e-13
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y  QV++N    A+ L   G ++VS GT
Sbjct:  282 PEFKTYSAQVIANAQALARQLQARGLKIVSDGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SYNE7 (Serine hydroxymethyltransferase OS=Synechococcus elongatus (strain PCC 7942) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.077e-13
Identity = 34/69 (49.28%), Postives = 48/69 (69.57%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L++  + G   + ++  V+I ANKNTVP D  S  V  GIR+GT A+T+RGF E +FA VA
Sbjct:  315 DNHLLLVDLRSIGMTGKVADLLVSDVNITANKNTVPFDPESPFVTSGIRLGTAAMTTRGFKEAEFAIVA 383

HSP 2 Score: 32.7278 bits (73), Expect = 2.077e-13
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y  QV++N    A+ L   G ++VS GT
Sbjct:  282 PEFKTYSAQVIANAQALARQLQARGLKIVSDGT 314
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_ANAVT1.599e-1344.12Serine hydroxymethyltransferase OS=Anabaena variab... [more]
GLYA_ACIC11.599e-1339.51Serine hydroxymethyltransferase OS=Acidothermus ce... [more]
GLYA1_PSESM1.600e-1346.67Serine hydroxymethyltransferase 1 OS=Pseudomonas s... [more]
GLYA2_PSEF51.600e-1346.67Serine hydroxymethyltransferase 2 OS=Pseudomonas f... [more]
GLYA_HALMA1.600e-1352.11Serine hydroxymethyltransferase OS=Haloarcula mari... [more]
GLYA_BACWK1.600e-1344.93Serine hydroxymethyltransferase OS=Bacillus weihen... [more]
GLYA1_RHILO2.076e-1338.64Serine hydroxymethyltransferase 1 OS=Rhizobium lot... [more]
GLYA2_VIBVY2.076e-1342.25Serine hydroxymethyltransferase 2 OS=Vibrio vulnif... [more]
GLYA_SYNP62.077e-1349.28Serine hydroxymethyltransferase OS=Synechococcus s... [more]
GLYA_SYNE72.077e-1349.28Serine hydroxymethyltransferase OS=Synechococcus e... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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