BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_KOSOT (Serine hydroxymethyltransferase OS=Kosmotoga olearia (strain TBF 19.5.1) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.078e-13
Identity = 35/85 (41.18%), Postives = 49/85 (57.65%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSE 357
            + HL LV+L  K + G   EK LE+  I  NKNT+P +  S  V  GIR+GTPA+T+RG  E +   +A   +  VK+   I+ E
Sbjct:  311 DTHLFLVDLNPKNVTGKAAEKALESADITVNKNTIPKETRSPFVTSGIRIGTPAVTTRGMGESEMKVIA---ELIVKVIDNIQDE 392

HSP 2 Score: 32.7278 bits (73), Expect = 2.078e-13
Identity = 13/32 (40.62%), Postives = 21/32 (65.62%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K YQ ++++N    A+ L  +G  +VSGGT
Sbjct:  279 EFKEYQMRIITNAKALAKELENLGLRIVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BAUCH (Serine hydroxymethyltransferase OS=Baumannia cicadellinicola subsp. Homalodisca coagulata GN=glyA PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.078e-13
Identity = 31/69 (44.93%), Postives = 43/69 (62.32%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL L++L +K I G   +  L   +I  NKN +P D  S+ V  GIR+GTPA+T RGF E D  ++A
Sbjct:  315 DNHLFLLDLVDKNITGQEADAALSYTNITVNKNIIPNDPQSSFVTSGIRIGTPAITRRGFNETDAYQLA 383

HSP 2 Score: 34.6538 bits (78), Expect = 2.078e-13
Identity = 14/33 (42.42%), Postives = 18/33 (54.55%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ QV+ N        L   + +VSGGT
Sbjct:  282 PEFKIYQHQVVKNAKTMVNVFLNRNFNVVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_NITOC (Serine hydroxymethyltransferase OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=glyA PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 2.078e-13
Identity = 30/69 (43.48%), Postives = 43/69 (62.32%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ++HL LV+L  K + G   +  L   +I  NKNTVP D  S  V  GIR+G+PA+T+RGF E +  ++A
Sbjct:  315 DSHLFLVDLVAKGLTGKAADAALGRANITVNKNTVPNDPQSPFVTSGIRIGSPAMTTRGFKEAEICELA 383

HSP 2 Score: 37.3502 bits (85), Expect = 2.078e-13
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ QV+ N    A+ +   GY++VSGGT
Sbjct:  282 PAFKDYQRQVIRNAQAMAEAIQSRGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA2_VIBVU (Serine hydroxymethyltransferase 2 OS=Vibrio vulnificus GN=glyA2 PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.697e-13
Identity = 30/71 (42.25%), Postives = 46/71 (64.79%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            + HL+LV+L+ K + G++ E+ LE   I  NKN +P D    M+  G+R+GTPA TSRGF  E+F  + ++
Sbjct:  324 DTHLMLVDLRPKGLKGNKAEEALERAGITCNKNGIPFDTEKPMITSGVRLGTPAGTSRGFGAEEFKLIGHW 394

HSP 2 Score: 32.3426 bits (72), Expect = 2.697e-13
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V+ N    A+ L   G ++V+GGT
Sbjct:  291 PEFKTYIDSVIDNAKVLAEVLQTRGCDIVTGGT 323
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BEII9 (Serine hydroxymethyltransferase OS=Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) GN=glyA PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.697e-13
Identity = 33/69 (47.83%), Postives = 43/69 (62.32%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L+ K++ G   E  L    I  NKN VP D  S  V  GIR+G+PA TSRGF  ++F  VA
Sbjct:  326 DNHLMLVDLRPKQLTGKAAEAALGRASITCNKNGVPFDTASPFVTSGIRLGSPAATSRGFGTKEFQDVA 394

HSP 2 Score: 33.4982 bits (75), Expect = 2.697e-13
Identity = 13/33 (39.39%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P+++ Y +QV+ N    A  L+E G+ + SGGT
Sbjct:  293 PDFRLYAQQVVVNAGTLASRLVEKGFAISSGGT 325
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_AGRRK (Serine hydroxymethyltransferase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) GN=glyA PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.697e-13
Identity = 29/68 (42.65%), Postives = 42/68 (61.76%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHL+LV+L+ K   G R E  L   ++  NKN +P D     V  G+R+GTPA T+RGF E +F ++
Sbjct:  320 DNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFKEAEFREI 387

HSP 2 Score: 34.2686 bits (77), Expect = 2.697e-13
Identity = 13/33 (39.39%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE++ Y  Q++ N    ++TL+  G ++VSGGT
Sbjct:  287 PEFQDYAAQIVKNAKALSETLISGGVDVVSGGT 319
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA1_HAHCH (Serine hydroxymethyltransferase 1 OS=Hahella chejuensis (strain KCTC 2396) GN=glyA1 PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 2.698e-13
Identity = 29/69 (42.03%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL L++L +K + G   +  L   +I  NKNTVP D  S  V  G+R+GTP +T+RGF E++  ++A
Sbjct:  315 DNHLFLIDLIDKGVTGKDADAALGRAYITVNKNTVPNDPQSPFVTSGLRIGTPGVTTRGFKEKEVVELA 383

HSP 2 Score: 35.039 bits (79), Expect = 2.698e-13
Identity = 14/33 (42.42%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P ++ YQ+QV++N    A  +   GY++VSGGT
Sbjct:  282 PAFRDYQQQVVNNARAMADAVKARGYKVVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_PASMU (Serine hydroxymethyltransferase OS=Pasteurella multocida GN=glyA PE=3 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.699e-13
Identity = 30/69 (43.48%), Postives = 42/69 (60.87%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ENHL LV+L +  + G   +  L + +I  NKN VP D     V  GIR+GTP++T RGF E + A++A
Sbjct:  315 ENHLFLVDLVSHGLTGKAADAALGSANITVNKNAVPNDPQKPFVTSGIRVGTPSITRRGFKEAESAELA 383

HSP 2 Score: 37.3502 bits (85), Expect = 2.699e-13
Identity = 15/33 (45.45%), Postives = 19/33 (57.58%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PEYK YQ+QV+ N         + GY +VS GT
Sbjct:  282 PEYKVYQQQVVKNAKAMVDVFKQRGYNVVSNGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DESOH (Serine hydroxymethyltransferase OS=Desulfococcus oleovorans (strain DSM 6200 / Hxd3) GN=glyA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.699e-13
Identity = 28/69 (40.58%), Postives = 41/69 (59.42%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NH++L +L+N  + G   E  LE   +  NKN VP D  +  V  G+R+GTP +T+RG  E + A VA
Sbjct:  312 DNHMMLADLRNISVTGKAAETALERAGLTLNKNAVPFDTENPTVTSGVRIGTPVMTTRGMKEPEMAVVA 380

HSP 2 Score: 37.7354 bits (86), Expect = 2.699e-13
Identity = 18/34 (52.94%), Postives = 22/34 (64.71%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T  + AYQ+QV+ N    A  L+E G ELVSGGT
Sbjct:  278 TDGFVAYQQQVVKNARALAAHLMEQGIELVSGGT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BACC4 (Serine hydroxymethyltransferase OS=Bacillus cereus (strain B4264) GN=glyA PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.700e-13
Identity = 31/69 (44.93%), Postives = 46/69 (66.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+L++++N  I G   E VL+ V I  NKNT+P +  S  V  G+R+GT A+TSRGF  E+  ++A
Sbjct:  309 DNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFSLEEMDEIA 377

HSP 2 Score: 31.9574 bits (71), Expect = 2.700e-13
Identity = 12/32 (37.50%), Postives = 22/32 (68.75%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            ++K Y + +++N ++ A+ L + G  LVSGGT
Sbjct:  277 DFKTYAQNIINNANRLAEGLQKEGLTLVSGGT 308
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_KOSOT2.078e-1341.18Serine hydroxymethyltransferase OS=Kosmotoga olear... [more]
GLYA_BAUCH2.078e-1344.93Serine hydroxymethyltransferase OS=Baumannia cicad... [more]
GLYA_NITOC2.078e-1343.48Serine hydroxymethyltransferase OS=Nitrosococcus o... [more]
GLYA2_VIBVU2.697e-1342.25Serine hydroxymethyltransferase 2 OS=Vibrio vulnif... [more]
GLYA_BEII92.697e-1347.83Serine hydroxymethyltransferase OS=Beijerinckia in... [more]
GLYA_AGRRK2.697e-1342.65Serine hydroxymethyltransferase OS=Agrobacterium r... [more]
GLYA1_HAHCH2.698e-1342.03Serine hydroxymethyltransferase 1 OS=Hahella cheju... [more]
GLYA_PASMU2.699e-1343.48Serine hydroxymethyltransferase OS=Pasteurella mul... [more]
GLYA_DESOH2.699e-1340.58Serine hydroxymethyltransferase OS=Desulfococcus o... [more]
GLYA_BACC42.700e-1344.93Serine hydroxymethyltransferase OS=Bacillus cereus... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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