BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA1_SULDN (Serine hydroxymethyltransferase 1 OS=Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) GN=glyA1 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.700e-13
Identity = 43/106 (40.57%), Postives = 55/106 (51.89%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFA----KVAYFFDAA--VKLAVKIKSETQGTKLKDFVATTQS 402
            +NHLVL++  N+   G   +  L    I  NKNTVPG+  S  V  GIR+G+PALTSRG  E +F     K+A   D     KL   +K E +    K FV   QS
Sbjct:  309 DNHLVLLSFLNRDFSGKDADIALGNAGITVNKNTVPGETRSPFVTSGIRIGSPALTSRGMKEREFEFIANKIADVLDDINNTKLQESVKKELKELAQK-FVIYNQS 413

HSP 2 Score: 33.8834 bits (76), Expect = 2.700e-13
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K Y  QV +N     + L++ GY+LVSGGT
Sbjct:  277 EWKDYAVQVKANAKILGEVLVKRGYDLVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_MYCPE (Serine hydroxymethyltransferase OS=Mycoplasma penetrans GN=glyA PE=3 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 2.701e-13
Identity = 31/78 (39.74%), Postives = 50/78 (64.10%), Query Frame = 1 
Query:  106 ENHLVLVNL-KNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+ V+L  +K+I G  VE+ LE   I  NKN +P D+ SA  P GIR+G+ A+T+RGF  ++F ++  +    ++
Sbjct:  305 DNHLLSVDLYSSKQITGDLVEQWLEQAKIVVNKNLIPYDINSAKSPSGIRIGSAAMTTRGFTTKEFKQIGLWIHEIIE 382

HSP 2 Score: 35.4242 bits (80), Expect = 2.701e-13
Identity = 13/33 (39.39%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V+ N +  +QTL E+GY++++ G+
Sbjct:  272 PEFKTYIKNVIDNIAILSQTLKELGYKIIADGS 304
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SYNFM (Serine hydroxymethyltransferase OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) GN=glyA PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 2.701e-13
Identity = 31/79 (39.24%), Postives = 44/79 (55.70%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLA 339
            +NHL+LV+L  + + G   E+ L+   I  NKN++P D     V  GIR+GTPAL +RG +      VA F    +K A
Sbjct:  309 DNHLMLVDLTPQGVTGRVAEETLDKAGITVNKNSIPFDQQKPQVTSGIRIGTPALATRGILPHHMKAVAGFMHRGLKSA 387

HSP 2 Score: 38.891 bits (89), Expect = 2.701e-13
Identity = 16/33 (48.48%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ++V+++ +  AQ L  +GY LVSGGT
Sbjct:  276 PSFKEYQQRVVADAAALAQELKALGYRLVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA2_VIBPA (Serine hydroxymethyltransferase 2 OS=Vibrio parahaemolyticus GN=glyA2 PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 3.503e-13
Identity = 31/65 (47.69%), Postives = 43/65 (66.15%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDF 297
            + HL+LV+L+ K + G++ E+ LE   I  NKN +P D    M+  GIR+GTPA TSRGF  E+F
Sbjct:  324 DTHLMLVDLRPKGLKGNKAEEALERAGITCNKNGIPFDTEKPMITSGIRLGTPAGTSRGFGAEEF 388

HSP 2 Score: 32.7278 bits (73), Expect = 3.503e-13
Identity = 13/33 (39.39%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V++N    A+ L   G ++V+GGT
Sbjct:  291 PEFKTYIDSVINNAKVLAEVLQTRGCDIVTGGT 323
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_METS4 (Serine hydroxymethyltransferase OS=Methylobacterium sp. (strain 4-46) GN=glyA PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 3.503e-13
Identity = 32/68 (47.06%), Postives = 40/68 (58.82%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHL+LV+++ K + G   E  L    I  NKN VP D     V  GIR+GTPA TSRGF   +F KV
Sbjct:  325 DNHLMLVDMRPKNLTGKAAEAALSRAGITCNKNGVPFDPQKPTVTSGIRLGTPAATSRGFGVAEFKKV 392

HSP 2 Score: 35.4242 bits (80), Expect = 3.503e-13
Identity = 12/34 (35.29%), Postives = 23/34 (67.65%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +P++K Y +QV+ N    A T++  G+++ +GGT
Sbjct:  291 SPDFKIYAKQVVENAKALADTIISGGFDITTGGT 324
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA1_RHOPA (Serine hydroxymethyltransferase 1 OS=Rhodopseudomonas palustris GN=glyA1 PE=3 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.503e-13
Identity = 30/70 (42.86%), Postives = 43/70 (61.43%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAY 312
            +NHL+LV+L+ K + G+  EK L    I  NKN +P D     V  G+R+GTPA T+RGF   +F +V +
Sbjct:  324 DNHLMLVDLRPKGLKGNVSEKALVRAGITCNKNGIPFDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGH 393

HSP 2 Score: 37.7354 bits (86), Expect = 3.503e-13
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P++K Y + V+ N    A+TL   G++LVSGGT
Sbjct:  291 PDFKVYTKNVVENAKALAETLRSAGFDLVSGGT 323
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_PROM3 (Serine hydroxymethyltransferase OS=Prochlorococcus marinus (strain MIT 9303) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.505e-13
Identity = 34/69 (49.28%), Postives = 48/69 (69.57%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHLVL++L++  + G   + ++  V+I ANKNTVP D  S  V  G+R+GT ALT+RGF EE F +VA
Sbjct:  319 DNHLVLLDLRSIGMTGKVADLLVSDVNITANKNTVPFDPESPFVTSGLRLGTAALTTRGFDEEAFREVA 387

HSP 2 Score: 31.9574 bits (71), Expect = 3.505e-13
Identity = 15/33 (45.45%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+ AY  QV++N    A  + E G  +VSGGT
Sbjct:  286 PEFHAYSCQVVANAQVLAGRIQERGIAVVSGGT 318
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_PSEHT (Serine hydroxymethyltransferase OS=Pseudoalteromonas haloplanktis (strain TAC 125) GN=glyA PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.506e-13
Identity = 32/69 (46.38%), Postives = 43/69 (62.32%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ENHL L++L NK I G   +  L   HI  NKN+VP D  S  V  G+R+G+PA+T RGF E +  ++A
Sbjct:  316 ENHLFLLDLINKDITGKDADAALGNAHITVNKNSVPNDPRSPFVTSGLRIGSPAITRRGFKEAESKELA 384

HSP 2 Score: 33.4982 bits (75), Expect = 3.506e-13
Identity = 14/33 (42.42%), Postives = 19/33 (57.58%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ QV+ N       + E GY++VS  T
Sbjct:  283 PEFKVYQTQVVKNAQAMVAVMQERGYKIVSDKT 315
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_UNCTG (Serine hydroxymethyltransferase OS=Uncultured termite group 1 bacterium phylotype Rs-D17 GN=glyA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 3.506e-13
Identity = 29/85 (34.12%), Postives = 49/85 (57.65%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSA-MVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSE 357
            ++H+ LV+L+   + G   +  LE   I  NKN +P D+    +  GIR+G+PA+T+RG  E +  K+A   +A +K+   I +E
Sbjct:  310 DSHMFLVDLRPLNVKGKNAQDTLEKAGITLNKNGIPYDLEKPTMTSGIRIGSPAVTTRGMKEPEMVKIA---EAIIKVLKNIDNE 391

HSP 2 Score: 40.0466 bits (92), Expect = 3.506e-13
Identity = 18/33 (54.55%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ+QVL+N  + A+TL E   ++VSGGT
Sbjct:  277 PEFKEYQKQVLANAKQLAETLEEGKLKIVSGGT 309
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BACCZ (Serine hydroxymethyltransferase OS=Bacillus cereus (strain ZK / E33L) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.507e-13
Identity = 31/69 (44.93%), Postives = 46/69 (66.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+L++++N  I G   E VL+ V I  NKNT+P +  S  V  G+R+GT A+TSRGF  E+  ++A
Sbjct:  310 DNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEEMDEIA 378

HSP 2 Score: 31.9574 bits (71), Expect = 3.507e-13
Identity = 12/32 (37.50%), Postives = 22/32 (68.75%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            ++K Y + +++N ++ A+ L + G  LVSGGT
Sbjct:  278 DFKTYAQNIINNANRLAEGLQKEGLTLVSGGT 309
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA1_SULDN2.700e-1340.57Serine hydroxymethyltransferase 1 OS=Sulfurimonas ... [more]
GLYA_MYCPE2.701e-1339.74Serine hydroxymethyltransferase OS=Mycoplasma pene... [more]
GLYA_SYNFM2.701e-1339.24Serine hydroxymethyltransferase OS=Syntrophobacter... [more]
GLYA2_VIBPA3.503e-1347.69Serine hydroxymethyltransferase 2 OS=Vibrio paraha... [more]
GLYA_METS43.503e-1347.06Serine hydroxymethyltransferase OS=Methylobacteriu... [more]
GLYA1_RHOPA3.503e-1342.86Serine hydroxymethyltransferase 1 OS=Rhodopseudomo... [more]
GLYA_PROM33.505e-1349.28Serine hydroxymethyltransferase OS=Prochlorococcus... [more]
GLYA_PSEHT3.506e-1346.38Serine hydroxymethyltransferase OS=Pseudoalteromon... [more]
GLYA_UNCTG3.506e-1334.12Serine hydroxymethyltransferase OS=Uncultured term... [more]
GLYA_BACCZ3.507e-1344.93Serine hydroxymethyltransferase OS=Bacillus cereus... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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