BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ANASK (Serine hydroxymethyltransferase OS=Anaeromyxobacter sp. (strain K) GN=glyA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 4.554e-13
Identity = 30/69 (43.48%), Postives = 41/69 (59.42%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L+ K++ G   E+ L    I  NKN +P D    M   GIR+GTPALT+RG    +   VA
Sbjct:  312 DNHLMLVDLRPKKLTGKIAEEALGRAGITVNKNMIPWDPEKPMTTSGIRVGTPALTTRGMGSREMTLVA 380

HSP 2 Score: 36.965 bits (84), Expect = 4.554e-13
Identity = 16/33 (48.48%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+KAYQ +++ N    A+ L   G  LVSGGT
Sbjct:  279 PEFKAYQRRIVENAQVLAEGLKSAGLRLVSGGT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ANAD2 (Serine hydroxymethyltransferase OS=Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) GN=glyA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 4.554e-13
Identity = 30/69 (43.48%), Postives = 41/69 (59.42%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L+ K++ G   E+ L    I  NKN +P D    M   GIR+GTPALT+RG    +   VA
Sbjct:  312 DNHLMLVDLRPKKLTGKIAEEALGKAGITVNKNMIPWDPEKPMTTSGIRVGTPALTTRGMGSREMTLVA 380

HSP 2 Score: 36.965 bits (84), Expect = 4.554e-13
Identity = 16/33 (48.48%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+KAYQ +++ N    A+ L   G  LVSGGT
Sbjct:  279 PEFKAYQRRIVENAQVLAEGLKSAGLRLVSGGT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DEIGD (Serine hydroxymethyltransferase OS=Deinococcus geothermalis (strain DSM 11300) GN=glyA PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 4.556e-13
Identity = 30/77 (38.96%), Postives = 52/77 (67.53%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGG-IRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL +++L+ + ++G++  + L+A  I  +K+T+P D   ++ GG IR+GTPA+T+RG  E D  +VA   D A+K
Sbjct:  318 DNHLFVLDLRPQGLNGTKATRRLDANDITISKSTLPYDTEKILHGGGIRIGTPAITTRGMKEADMERVADLIDRALK 394

HSP 2 Score: 33.4982 bits (75), Expect = 4.556e-13
Identity = 14/33 (42.42%), Postives = 18/33 (54.55%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y  Q++ N    A      GY +VSGGT
Sbjct:  285 PEFKDYAAQIIRNAQALAGAFQNRGYRVVSGGT 317
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA2_VIBCH (Serine hydroxymethyltransferase 2 OS=Vibrio cholerae GN=glyA2 PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 5.908e-13
Identity = 32/65 (49.23%), Postives = 44/65 (67.69%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDF 297
            + HL+LV+L+ K + G++VE+ LE   I  NKN +P D    M+  GIR+GTPA TSRGF  E+F
Sbjct:  328 DTHLMLVDLRPKGLKGNQVEQALERAGITCNKNGIPFDEEKPMITSGIRLGTPAGTSRGFGREEF 392

HSP 2 Score: 31.187 bits (69), Expect = 5.908e-13
Identity = 12/33 (36.36%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE++ Y + V+ N    A+ L   G ++V+GGT
Sbjct:  295 PEFRTYIDSVIDNAKVLAEVLQTRGCDIVTGGT 327
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CYTH3 (Serine hydroxymethyltransferase OS=Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) GN=glyA PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 5.909e-13
Identity = 30/76 (39.47%), Postives = 44/76 (57.89%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVP-GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAV 330
            +NHL+L++L++K + G   E  L    I  NKN VP  D S  V  G+R+GT A+TSRG V +D  ++    D  +
Sbjct:  329 DNHLMLIDLRSKNLTGKEAENALIRADITINKNMVPFDDKSPFVTSGMRVGTAAITSRGMVGDDMIRIVEMIDTVL 404

HSP 2 Score: 32.7278 bits (73), Expect = 5.909e-13
Identity = 12/32 (37.50%), Postives = 20/32 (62.50%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +Y  Y +Q+  N    A+  L+ GY+++SGGT
Sbjct:  297 DYLQYAKQIQKNAQIMAEAFLKKGYDIISGGT 328
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SYNPX (Serine hydroxymethyltransferase OS=Synechococcus sp. (strain WH8102) GN=glyA PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.910e-13
Identity = 32/69 (46.38%), Postives = 46/69 (66.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NH+VL++L++  + G   + ++  VHI ANKNTVP D  S  V  G+R+GT ALT+RGF    F +VA
Sbjct:  319 DNHVVLLDLRSVGMTGKVADLLVSDVHITANKNTVPFDPESPFVTSGLRLGTAALTTRGFDAGAFREVA 387

HSP 2 Score: 34.2686 bits (77), Expect = 5.910e-13
Identity = 13/33 (39.39%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K Y + V++N +  A+ L+  G ++VSGGT
Sbjct:  286 PAFKTYSQHVVANAAALAERLIARGIDVVSGGT 318
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_RHOCS (Serine hydroxymethyltransferase OS=Rhodospirillum centenum (strain ATCC 51521 / SW) GN=glyA PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.910e-13
Identity = 30/68 (44.12%), Postives = 44/68 (64.71%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKV 306
            ++H+VLV+L+ KR+ G   E  LE   +  NKN VP D    +V  G+R+G+PA T+RGF   +FA+V
Sbjct:  320 DSHIVLVDLRPKRLTGKAAEATLEHAGMTCNKNGVPFDPEKPLVTSGVRLGSPAATTRGFGTAEFAQV 387

HSP 2 Score: 34.2686 bits (77), Expect = 5.910e-13
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+KAY + V+ N    A  L+  G ++VSGGT
Sbjct:  287 PEFKAYAQAVVDNARVLADRLVAGGLDIVSGGT 319
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_PARD8 (Serine hydroxymethyltransferase OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) GN=glyA PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 5.911e-13
Identity = 32/78 (41.03%), Postives = 43/78 (55.13%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRID--GSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAV 330
            +NH +L++L+ K  D  G   EK L A  I  NKN VP D  SA    GIR+GTPA+T+RG  E    ++    D  +
Sbjct:  321 DNHSMLIDLRTKFPDLTGKVAEKALVAADITVNKNMVPFDSRSAFQTSGIRVGTPAITTRGAKEPLMGEIVELIDTVL 398

HSP 2 Score: 40.817 bits (94), Expect = 5.911e-13
Identity = 16/33 (48.48%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PEYK YQ QV +N +  A+   + GY+++SGGT
Sbjct:  288 PEYKTYQAQVKANAAAMAKAFTDKGYKIISGGT 320
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_FLAJ1 (Serine hydroxymethyltransferase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) GN=glyA PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 5.912e-13
Identity = 31/73 (42.47%), Postives = 43/73 (58.90%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVP-GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFD 321
            +NH++L++L+NK I G   E  L    I  NKN VP  D S  +  GIR+GT A+T+RG VE+D   +    D
Sbjct:  321 DNHMMLIDLRNKNISGKEAENALVKAEITVNKNMVPFDDKSPFITSGIRVGTAAITTRGLVEKDMETIVALID 393

HSP 2 Score: 28.8758 bits (63), Expect = 5.912e-13
Identity = 11/32 (34.38%), Postives = 18/32 (56.25%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+  Y  Q+  N +  A   ++ GY ++SGGT
Sbjct:  289 EFFTYAMQLQKNANAMADAFVKRGYNIISGGT 320
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ACIF5 (Serine hydroxymethyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=glyA PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 5.916e-13
Identity = 35/84 (41.67%), Postives = 47/84 (55.95%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSA-MVPGGIRMGTPALTSRGFVEEDFAK----VAYFFDAAVKLAV 342
            +NHL L+NL  K + G   E+ L   +I  NKN VP D+    V  GIR+GTPA T+RGF E +  +    +A   DA+   AV
Sbjct:  313 DNHLFLLNLGEK-VTGKEAEEALGQANITVNKNAVPFDIRPPAVTSGIRIGTPAATTRGFGEAEMHRLGNGIADVLDASSDAAV 395

HSP 2 Score: 38.1206 bits (87), Expect = 5.916e-13
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K+YQ+QV+ N    ++ L   GY  VSGGT
Sbjct:  280 PEFKSYQQQVIHNAQTLSKVLAGRGYGAVSGGT 312
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_ANASK4.554e-1343.48Serine hydroxymethyltransferase OS=Anaeromyxobacte... [more]
GLYA_ANAD24.554e-1343.48Serine hydroxymethyltransferase OS=Anaeromyxobacte... [more]
GLYA_DEIGD4.556e-1338.96Serine hydroxymethyltransferase OS=Deinococcus geo... [more]
GLYA2_VIBCH5.908e-1349.23Serine hydroxymethyltransferase 2 OS=Vibrio choler... [more]
GLYA_CYTH35.909e-1339.47Serine hydroxymethyltransferase OS=Cytophaga hutch... [more]
GLYA_SYNPX5.910e-1346.38Serine hydroxymethyltransferase OS=Synechococcus s... [more]
GLYA_RHOCS5.910e-1344.12Serine hydroxymethyltransferase OS=Rhodospirillum ... [more]
GLYA_PARD85.911e-1341.03Serine hydroxymethyltransferase OS=Parabacteroides... [more]
GLYA_FLAJ15.912e-1342.47Serine hydroxymethyltransferase OS=Flavobacterium ... [more]
GLYA_ACIF55.916e-1341.67Serine hydroxymethyltransferase OS=Acidithiobacill... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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