BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ACIF2 (Serine hydroxymethyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=glyA PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 5.916e-13
Identity = 35/84 (41.67%), Postives = 47/84 (55.95%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSA-MVPGGIRMGTPALTSRGFVEEDFAK----VAYFFDAAVKLAV 342
            +NHL L+NL  K + G   E+ L   +I  NKN VP D+    V  GIR+GTPA T+RGF E +  +    +A   DA+   AV
Sbjct:  313 DNHLFLLNLGEK-VTGKEAEEALGQANITVNKNAVPFDIRPPAVTSGIRIGTPAATTRGFGEAEMHRLGNGIADVLDASSDAAV 395

HSP 2 Score: 38.1206 bits (87), Expect = 5.916e-13
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K+YQ+QV+ N    ++ L   GY  VSGGT
Sbjct:  280 PEFKSYQQQVIHNAQTLSKVLAGRGYGAVSGGT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_RHOP2 (Serine hydroxymethyltransferase OS=Rhodopseudomonas palustris (strain HaA2) GN=glyA PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 7.674e-13
Identity = 30/68 (44.12%), Postives = 42/68 (61.76%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHL+LV+L+ K + G+  EK L    I  NKN +P D     V  G+R+GTPA T+RGF   +F +V
Sbjct:  325 DNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIPFDPEKPFVTSGLRLGTPAATTRGFGVAEFQQV 392

HSP 2 Score: 37.3502 bits (85), Expect = 7.674e-13
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P++K Y + V+ N    A+TL   G++LVSGGT
Sbjct:  292 PDFKVYAKNVVENAKALAETLRAAGFDLVSGGT 324
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA2_SULDN (Serine hydroxymethyltransferase 2 OS=Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) GN=glyA2 PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 7.680e-13
Identity = 31/69 (44.93%), Postives = 41/69 (59.42%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHLVLV+  N+   G   +  LE   I  NKN VP D  S  +  GIR+G+PALT+RG  E +F  +A
Sbjct:  314 DNHLVLVSFLNRDFSGKDADAALERAGITVNKNGVPNDTRSPFITSGIRIGSPALTARGMKEAEFELIA 382

HSP 2 Score: 32.7278 bits (73), Expect = 7.680e-13
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P ++ Y +QV +N       L + GY+LVSGGT
Sbjct:  281 PSWREYAKQVKANAKVLEVVLTKRGYDLVSGGT 313
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DECAR (Serine hydroxymethyltransferase OS=Dechloromonas aromatica (strain RCB) GN=glyA PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 7.682e-13
Identity = 29/70 (41.43%), Postives = 43/70 (61.43%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAY 312
            E+H+ L++L+ K I G   E  L   HI  NKN +P D     V  GIR+G+PA+T+RGF E +  ++A+
Sbjct:  313 ESHVFLLDLRAKNITGKDAEAALGRAHITVNKNGIPNDPQKPFVTSGIRIGSPAMTTRGFTEIEAEQIAH 382

HSP 2 Score: 34.2686 bits (77), Expect = 7.682e-13
Identity = 18/35 (51.43%), Postives = 23/35 (65.71%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTL-LEMGYELVSGGT 104
            TPE+K YQEQV++N    A+ L  E G  +VSG T
Sbjct:  278 TPEFKNYQEQVINNARVMARVLGEERGLRIVSGRT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA1_ERWCT (Serine hydroxymethyltransferase 1 OS=Erwinia carotovora subsp. atroseptica GN=glyA1 PE=3 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 7.682e-13
Identity = 30/68 (44.12%), Postives = 41/68 (60.29%), Query Frame = 1 
Query:  109 NHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            NHL L++L +K + G   +  L   +I  NKN+VP D  S  V  GIR+GTPA T RGF E +  ++A
Sbjct:  316 NHLFLLDLVSKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRIGTPAATRRGFKEAEVRELA 383

HSP 2 Score: 35.8094 bits (81), Expect = 7.682e-13
Identity = 14/33 (42.42%), Postives = 19/33 (57.58%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ+QV  N     +  L  G+ +VSG T
Sbjct:  282 PEFKVYQQQVAKNAKAMVEVFLSRGFNVVSGAT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BACCN (Serine hydroxymethyltransferase OS=Bacillus cereus subsp. cytotoxis (strain NVH 391-98) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 7.683e-13
Identity = 31/69 (44.93%), Postives = 46/69 (66.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+L++++N  I G   E VL+ V I  NKNT+P +  S  V  G+R+GT A+TSRGF  E+  ++A
Sbjct:  309 DNHLILIDVRNLNITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEEMDEIA 377

HSP 2 Score: 30.8018 bits (68), Expect = 7.683e-13
Identity = 12/32 (37.50%), Postives = 20/32 (62.50%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            ++K Y + ++ N  + A+ L + G  LVSGGT
Sbjct:  277 DFKTYAQNIIHNAQRLAEGLQKEGLTLVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_OCEIH (Serine hydroxymethyltransferase OS=Oceanobacillus iheyensis GN=glyA PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 7.685e-13
Identity = 35/100 (35.00%), Postives = 57/100 (57.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVATTQS 402
            +NHL+L+++   ++ G   EK L+ + I  NKNT+P D  S  V  GIR+GT A+T+RGF EE+  ++A        +++ +K      KLK+     Q+
Sbjct:  309 DNHLLLLDVTPLQLTGKVAEKALDDIGITTNKNTIPFDQESPFVTSGIRIGTAAVTTRGFGEEEMKEIASI------ISLTLKHHEDEAKLKEAAQRVQA 402

HSP 2 Score: 31.5722 bits (70), Expect = 7.685e-13
Identity = 12/32 (37.50%), Postives = 21/32 (65.62%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            ++KAY +Q+++N     + L + G  +VSGGT
Sbjct:  277 DFKAYSKQIVANAKLLGEALNKEGIRIVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_RHOPB (Serine hydroxymethyltransferase OS=Rhodopseudomonas palustris (strain BisB18) GN=glyA PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 9.961e-13
Identity = 31/68 (45.59%), Postives = 42/68 (61.76%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHL+LV+L+ K + G+  EK L    I  NKN +P D     V  GIR+GTPA T+RGF   +F +V
Sbjct:  332 DNHLMLVDLRPKSLKGNVSEKALVRAGITCNKNGIPYDPEKPFVTSGIRLGTPAATTRGFGVAEFQQV 399

HSP 2 Score: 35.8094 bits (81), Expect = 9.961e-13
Identity = 14/33 (42.42%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P++K Y + V+ N    A+TL   G+++VSGGT
Sbjct:  299 PDFKVYAKNVVENAKALAETLRGHGFDIVSGGT 331
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA1_RHORT (Serine hydroxymethyltransferase 1 OS=Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255) GN=glyA1 PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 9.964e-13
Identity = 28/67 (41.79%), Postives = 43/67 (64.18%), Query Frame = 1 
Query:  112 HLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            HL +V+L+   + G+  E+ LE++ I  NKN +P D    MV  GIR+G+ A TSRGF  E++ ++A
Sbjct:  331 HLAVVDLRPLGVTGTIAEQALESIGITLNKNAIPNDPEKPMVTSGIRVGSAAGTSRGFGPEEYRRIA 397

HSP 2 Score: 37.7354 bits (86), Expect = 9.964e-13
Identity = 16/33 (48.48%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K Y  +V+ NC   AQTLL+ G  + SGGT
Sbjct:  296 PSFKIYARRVVENCRVLAQTLLDGGLAITSGGT 328
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SILPO (Serine hydroxymethyltransferase OS=Silicibacter pomeroyi GN=glyA1 PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 9.966e-13
Identity = 32/69 (46.38%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            + H+VLV+L+ K + G+  EK L   HI  NKN VP D     V  GIR+G+PA T+RGF E +F ++A
Sbjct:  321 DTHVVLVDLRPKGVKGNATEKALGRAHITCNKNGVPFDPEKPTVTSGIRLGSPAGTTRGFAETEFRQIA 389

HSP 2 Score: 30.4166 bits (67), Expect = 9.966e-13
Identity = 12/33 (36.36%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y +QV++N    +  L++ G + V+ GT
Sbjct:  288 PEFKTYIQQVIANAQALSDQLIKGGLDTVTHGT 320
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_ACIF25.916e-1341.67Serine hydroxymethyltransferase OS=Acidithiobacill... [more]
GLYA_RHOP27.674e-1344.12Serine hydroxymethyltransferase OS=Rhodopseudomona... [more]
GLYA2_SULDN7.680e-1344.93Serine hydroxymethyltransferase 2 OS=Sulfurimonas ... [more]
GLYA_DECAR7.682e-1341.43Serine hydroxymethyltransferase OS=Dechloromonas a... [more]
GLYA1_ERWCT7.682e-1344.12Serine hydroxymethyltransferase 1 OS=Erwinia carot... [more]
GLYA_BACCN7.683e-1344.93Serine hydroxymethyltransferase OS=Bacillus cereus... [more]
GLYA_OCEIH7.685e-1335.00Serine hydroxymethyltransferase OS=Oceanobacillus ... [more]
GLYA_RHOPB9.961e-1345.59Serine hydroxymethyltransferase OS=Rhodopseudomona... [more]
GLYA1_RHORT9.964e-1341.79Serine hydroxymethyltransferase 1 OS=Rhodospirillu... [more]
GLYA_SILPO9.966e-1346.38Serine hydroxymethyltransferase OS=Silicibacter po... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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