BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CAMJE (Serine hydroxymethyltransferase OS=Campylobacter jejuni GN=glyA PE=1 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 1.683e-12
Identity = 30/71 (42.25%), Postives = 46/71 (64.79%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            +NHLVL++  ++   G   +  L    I ANKNTVPG++ S  +  G+R+GTPALT+RGF E++   V+ +
Sbjct:  308 DNHLVLMSFLDREFSGKDADLALGNAGITANKNTVPGEIRSPFITSGLRLGTPALTARGFKEKEMEIVSNY 378

HSP 2 Score: 30.0314 bits (66), Expect = 1.683e-12
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K Y +QV +N    A  L++  ++LVS GT
Sbjct:  276 EWKVYAKQVRTNAQVLANVLMDRKFKLVSDGT 307
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CAMJ8 (Serine hydroxymethyltransferase OS=Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.683e-12
Identity = 30/71 (42.25%), Postives = 46/71 (64.79%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            +NHLVL++  ++   G   +  L    I ANKNTVPG++ S  +  G+R+GTPALT+RGF E++   V+ +
Sbjct:  308 DNHLVLMSFLDREFSGKDADLALGNAGITANKNTVPGEIRSPFITSGLRLGTPALTARGFKEKEMEIVSNY 378

HSP 2 Score: 30.0314 bits (66), Expect = 1.683e-12
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K Y +QV +N    A  L++  ++LVS GT
Sbjct:  276 EWKVYAKQVRTNAQVLANVLMDRKFKLVSDGT 307
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LEPBL (Serine hydroxymethyltransferase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain L550) GN=glyA PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.683e-12
Identity = 28/68 (41.18%), Postives = 40/68 (58.82%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVS-AMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHLVL+++  K + G +    L+ V +  NKN +P D +   V  GIR+GTPALT+RG    D   V
Sbjct:  309 DNHLVLLDVSVKGLTGVQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETV 376

HSP 2 Score: 40.0466 bits (92), Expect = 1.683e-12
Identity = 18/33 (54.55%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PEYK Y E VL+N    A+  L+ GY +VSGGT
Sbjct:  276 PEYKKYIEIVLANAKTLAEVFLKRGYRVVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LEPBJ (Serine hydroxymethyltransferase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) GN=glyA PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.683e-12
Identity = 28/68 (41.18%), Postives = 40/68 (58.82%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVS-AMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHLVL+++  K + G +    L+ V +  NKN +P D +   V  GIR+GTPALT+RG    D   V
Sbjct:  309 DNHLVLLDVSVKGLTGVQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETV 376

HSP 2 Score: 40.0466 bits (92), Expect = 1.683e-12
Identity = 18/33 (54.55%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PEYK Y E VL+N    A+  L+ GY +VSGGT
Sbjct:  276 PEYKKYIEIVLANAKTLAEVFLKRGYRVVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BART1 (Serine hydroxymethyltransferase OS=Bartonella tribocorum (strain CIP 105476 / IBS 506) GN=glyA PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.799e-12
Identity = 43/122 (35.25%), Postives = 66/122 (54.10%), Query Frame = 1 
Query:   40 AIARNLHRLCSRWAMNLFLVEPENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFF---------------DAAVKLAVKIKSE 357
            A A+ L +       N+     +NHL+LV+L++K + G R E  L   HI  NKN +P D  +  +  GIR+G+PA T+RGF+E++F +VA+                 + AV++AVK K E
Sbjct:  301 ANAKTLAKTLQSNGFNIVSGGTDNHLLLVDLRSKNLTGKRAELALGRAHITCNKNGIPFDPETPSITSGIRLGSPAATTRGFLEKEFVQVAHLIAEVLDGLRNAKSDEDNHAVEMAVKKKVE 422
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CAMJJ (Serine hydroxymethyltransferase OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 2.185e-12
Identity = 30/71 (42.25%), Postives = 46/71 (64.79%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            +NHLVL++  ++   G   +  L    I ANKNTVPG++ S  +  G+R+GTPALT+RGF E++   V+ +
Sbjct:  308 DNHLVLMSFLDREFSGKDADLALGNAGITANKNTVPGEIRSPFITSGLRLGTPALTARGFKEKEMEIVSNY 378

HSP 2 Score: 29.6462 bits (65), Expect = 2.185e-12
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K Y +QV +N    A  L++  ++LVS GT
Sbjct:  276 EWKIYAKQVRTNAQVLANVLMDRKFKLVSDGT 307
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_HALSA (Serine hydroxymethyltransferase OS=Halobacterium salinarium GN=glyA PE=3 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 2.185e-12
Identity = 41/98 (41.84%), Postives = 53/98 (54.08%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKR--IDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVA 390
            + HLVLV+L+     I G  VE  LE V I  N NTVP +  SA  P GIR+GTPALT+RGF  +    VA   D   ++   +  E+    + D VA
Sbjct:  311 DTHLVLVDLRESHPDISGGDVEGELEDVGIVLNANTVPDETRSAFDPSGIRIGTPALTTRGFDADAMETVA---DCIARVIDNLGDESVYADVADTVA 405

HSP 2 Score: 32.7278 bits (73), Expect = 2.185e-12
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P ++ Y  QV+ N +   + L E G+ LVSGGT
Sbjct:  278 PAFEEYAAQVVENAAVLGERLQEHGFSLVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_HALS3 (Serine hydroxymethyltransferase OS=Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) GN=glyA PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.185e-12
Identity = 41/98 (41.84%), Postives = 53/98 (54.08%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKR--IDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVA 390
            + HLVLV+L+     I G  VE  LE V I  N NTVP +  SA  P GIR+GTPALT+RGF  +    VA   D   ++   +  E+    + D VA
Sbjct:  311 DTHLVLVDLRESHPDISGGDVEGELEDVGIVLNANTVPDETRSAFDPSGIRIGTPALTTRGFDADAMETVA---DCIARVIDNLGDESVYADVADTVA 405

HSP 2 Score: 32.7278 bits (73), Expect = 2.185e-12
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P ++ Y  QV+ N +   + L E G+ LVSGGT
Sbjct:  278 PAFEEYAAQVVENAAVLGERLQEHGFSLVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LEPIN (Serine hydroxymethyltransferase OS=Leptospira interrogans GN=glyA PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.185e-12
Identity = 28/68 (41.18%), Postives = 41/68 (60.29%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVS-AMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHLVL+++  K + G++    L+ V +  NKN +P D +   V  GIR+GTPALT+RG    D   V
Sbjct:  309 DNHLVLLDVSVKGLTGAQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETV 376

HSP 2 Score: 38.5058 bits (88), Expect = 2.185e-12
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P+YK Y E VL+N    A+  ++ GY +VSGGT
Sbjct:  276 PDYKKYIETVLANAKTLAEVFVKRGYRVVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LEPIC (Serine hydroxymethyltransferase OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni GN=glyA PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.185e-12
Identity = 28/68 (41.18%), Postives = 41/68 (60.29%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVS-AMVPGGIRMGTPALTSRGFVEEDFAKV 306
            +NHLVL+++  K + G++    L+ V +  NKN +P D +   V  GIR+GTPALT+RG    D   V
Sbjct:  309 DNHLVLLDVSVKGLTGAQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETV 376

HSP 2 Score: 38.5058 bits (88), Expect = 2.185e-12
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P+YK Y E VL+N    A+  ++ GY +VSGGT
Sbjct:  276 PDYKKYIETVLANAKTLAEVFVKRGYRVVSGGT 308
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_CAMJE1.683e-1242.25Serine hydroxymethyltransferase OS=Campylobacter j... [more]
GLYA_CAMJ81.683e-1242.25Serine hydroxymethyltransferase OS=Campylobacter j... [more]
GLYA_LEPBL1.683e-1241.18Serine hydroxymethyltransferase OS=Leptospira borg... [more]
GLYA_LEPBJ1.683e-1241.18Serine hydroxymethyltransferase OS=Leptospira borg... [more]
GLYA_BART11.799e-1235.25Serine hydroxymethyltransferase OS=Bartonella trib... [more]
GLYA_CAMJJ2.185e-1242.25Serine hydroxymethyltransferase OS=Campylobacter j... [more]
GLYA_HALSA2.185e-1241.84Serine hydroxymethyltransferase OS=Halobacterium s... [more]
GLYA_HALS32.185e-1241.84Serine hydroxymethyltransferase OS=Halobacterium s... [more]
GLYA_LEPIN2.185e-1241.18Serine hydroxymethyltransferase OS=Leptospira inte... [more]
GLYA_LEPIC2.185e-1241.18Serine hydroxymethyltransferase OS=Leptospira inte... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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