BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig809 contig Csv1_Contig809:454..857. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_PEDPA (Serine hydroxymethyltransferase OS=Pediococcus pentosaceus (strain ATCC 25745 / 183-1w) GN=glyA PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.186e-12
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLA 339
            +NHL+ V+L   +++G  V+ +L+ V I  NK  +P + +S     GIR+GTPA+T+RGF E+D  +VA     A+K A
Sbjct:  309 DNHLMTVDLTGTQLNGKEVQNLLDGVFITTNKEAIPEEKLSPFKTSGIRIGTPAITTRGFKEDDCREVANLIIRAIKNA 387

HSP 2 Score: 28.1054 bits (61), Expect = 2.186e-12
Identity = 14/34 (41.18%), Postives = 20/34 (58.82%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTL-LEMGYELVSGGT 104
            PE+K YQ+QVL N    A+    +    +V+GGT
Sbjct:  275 PEFKTYQKQVLKNAKAMAEVFENDTILHVVTGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LISW6 (Serine hydroxymethyltransferase OS=Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.838e-12
Identity = 39/97 (40.21%), Postives = 54/97 (55.67%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVAT 393
            +NHL+L++LK   + G  VEKVL+ V I  NKNT+P +  S  V  GIR+G  A+T+RGF E    KV       +     I++E     +K  VAT
Sbjct:  309 DNHLLLIDLKPLSLTGKAVEKVLDEVGITVNKNTIPFETESPFVTSGIRVGVAAVTTRGFDEVAIEKVGVLISEVLH---NIENEEVLADVKARVAT 402

HSP 2 Score: 29.6462 bits (65), Expect = 2.838e-12
Identity = 12/33 (36.36%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+  Y EQ++ N  K A+TL      +++GG+
Sbjct:  276 PEFTTYCEQIIRNSKKLAETLQAHDVTVLTGGS 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_STRGG (Serine hydroxymethyltransferase OS=Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) GN=glyA PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.068e-12
Identity = 36/75 (48.00%), Postives = 48/75 (64.00%), Query Frame = 1 
Query:  112 HLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVSA-MVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            HLVLV+L+N  +DG + E  L  + I  N+N +P D    MV  G+R+GTPAL +RGF  EDF +VA    AA+K
Sbjct:  319 HLVLVDLRNSELDGQQAEDRLHELGITVNRNAIPNDPRPPMVTSGLRIGTPALATRGFGAEDFTEVAEIIAAALK 393
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LACSS (Serine hydroxymethyltransferase OS=Lactobacillus sakei subsp. sakei (strain 23K) GN=glyA PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.068e-12
Identity = 32/69 (46.38%), Postives = 51/69 (73.91%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+L++L N  ++G  ++ +L++VHI  NKNT+P + +S     GIR+GTPA+TSRGF E+D  ++A
Sbjct:  308 DNHLMLLDLTNSGLNGKELQNLLDSVHITVNKNTIPFEKLSPFKTSGIRIGTPAITSRGFKEKDCEQIA 376
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_OCHA4 (Serine hydroxymethyltransferase OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) GN=glyA PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.679e-12
Identity = 33/88 (37.50%), Postives = 49/88 (55.68%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQG 366
            +NHL+LV+L+ K   G R E  L   +I  NKN +P D     V  G+R+GTPA T+RGF   +F ++     A V   +K+ +  +G
Sbjct:  327 DNHLMLVDLRPKNATGKRAEAALGRANITCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFGVTEFKEIGSLI-AEVLDGLKVANSDEG 413

HSP 2 Score: 33.113 bits (74), Expect = 3.679e-12
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V+ N    A+ L   G ++VSGGT
Sbjct:  294 PEFKLYAKNVVDNARALAEELKSNGLDIVSGGT 326
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BRUSI (Serine hydroxymethyltransferase OS=Brucella suis (strain ATCC 23445 / NCTC 10510) GN=glyA PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.679e-12
Identity = 33/88 (37.50%), Postives = 49/88 (55.68%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQG 366
            +NHL+LV+L+ K   G R E  L   +I  NKN +P D     V  G+R+GTPA T+RGF   +F ++     A V   +K+ +  +G
Sbjct:  326 DNHLMLVDLRPKNATGKRAEAALGRANITCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFGVAEFKEIGSLI-AEVLDGLKVANSDEG 412

HSP 2 Score: 33.113 bits (74), Expect = 3.679e-12
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V+ N    A+ L   G ++VSGGT
Sbjct:  293 PEFKLYAKNVVDNARALAEELKSHGLDIVSGGT 325
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BRUO2 (Serine hydroxymethyltransferase OS=Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) GN=glyA PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.679e-12
Identity = 33/88 (37.50%), Postives = 49/88 (55.68%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQG 366
            +NHL+LV+L+ K   G R E  L   +I  NKN +P D     V  G+R+GTPA T+RGF   +F ++     A V   +K+ +  +G
Sbjct:  326 DNHLMLVDLRPKNATGKRAEAALGRANITCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFGVAEFKEIGSLI-AEVLDGLKVANSDEG 412

HSP 2 Score: 33.113 bits (74), Expect = 3.679e-12
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V+ N    A+ L   G ++VSGGT
Sbjct:  293 PEFKLYAKNVVDNARALAEELKSHGLDIVSGGT 325
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BRUME (Serine hydroxymethyltransferase OS=Brucella melitensis GN=glyA PE=3 SV=2)

HSP 1 Score: 58.151 bits (139), Expect = 3.679e-12
Identity = 33/88 (37.50%), Postives = 49/88 (55.68%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQG 366
            +NHL+LV+L+ K   G R E  L   +I  NKN +P D     V  G+R+GTPA T+RGF   +F ++     A V   +K+ +  +G
Sbjct:  326 DNHLMLVDLRPKNATGKRAEAALGRANITCNKNGIPFDPEKPFVASGVRLGTPAGTTRGFGVAEFKEIGSLI-AEVLDGLKVANSDEG 412

HSP 2 Score: 33.113 bits (74), Expect = 3.679e-12
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V+ N    A+ L   G ++VSGGT
Sbjct:  293 PEFKLYAKNVVDNARALAEELKSHGLDIVSGGT 325
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BRUMB (Serine hydroxymethyltransferase OS=Brucella melitensis biotype 2 (strain ATCC 23457) GN=glyA PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.679e-12
Identity = 33/88 (37.50%), Postives = 49/88 (55.68%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQG 366
            +NHL+LV+L+ K   G R E  L   +I  NKN +P D     V  G+R+GTPA T+RGF   +F ++     A V   +K+ +  +G
Sbjct:  326 DNHLMLVDLRPKNATGKRAEAALGRANITCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFGVAEFKEIGSLI-AEVLDGLKVANSDEG 412

HSP 2 Score: 33.113 bits (74), Expect = 3.679e-12
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V+ N    A+ L   G ++VSGGT
Sbjct:  293 PEFKLYAKNVVDNARALAEELKSHGLDIVSGGT 325
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_BRUAB (Serine hydroxymethyltransferase OS=Brucella abortus GN=glyA PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.679e-12
Identity = 33/88 (37.50%), Postives = 49/88 (55.68%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQG 366
            +NHL+LV+L+ K   G R E  L   +I  NKN +P D     V  G+R+GTPA T+RGF   +F ++     A V   +K+ +  +G
Sbjct:  326 DNHLMLVDLRPKNATGKRAEAALGRANITCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFGVAEFKEIGSLI-AEVLDGLKVANSDEG 412

HSP 2 Score: 33.113 bits (74), Expect = 3.679e-12
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y + V+ N    A+ L   G ++VSGGT
Sbjct:  293 PEFKLYAKNVVDNARALAEELKSHGLDIVSGGT 325
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_PEDPA2.186e-1240.51Serine hydroxymethyltransferase OS=Pediococcus pen... [more]
GLYA_LISW62.838e-1240.21Serine hydroxymethyltransferase OS=Listeria welshi... [more]
GLYA_STRGG3.068e-1248.00Serine hydroxymethyltransferase OS=Streptomyces gr... [more]
GLYA_LACSS3.068e-1246.38Serine hydroxymethyltransferase OS=Lactobacillus s... [more]
GLYA_OCHA43.679e-1237.50Serine hydroxymethyltransferase OS=Ochrobactrum an... [more]
GLYA_BRUSI3.679e-1237.50Serine hydroxymethyltransferase OS=Brucella suis (... [more]
GLYA_BRUO23.679e-1237.50Serine hydroxymethyltransferase OS=Brucella ovis (... [more]
GLYA_BRUME3.679e-1237.50Serine hydroxymethyltransferase OS=Brucella melite... [more]
GLYA_BRUMB3.679e-1237.50Serine hydroxymethyltransferase OS=Brucella melite... [more]
GLYA_BRUAB3.679e-1237.50Serine hydroxymethyltransferase OS=Brucella abortu... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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