BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig809 contig Csv1_Contig809:454..857. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_STRMK (Serine hydroxymethyltransferase OS=Stenotrophomonas maltophilia (strain K279a) GN=glyA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 3.446e-18
Identity = 32/69 (46.38%), Postives = 45/69 (65.22%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV++  K + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+VE+D   +A
Sbjct:  315 QNHLMLVDMIGKDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAVTTRGYVEQDCVDLA 383

HSP 2 Score: 45.4394 bits (106), Expect = 3.446e-18
Identity = 19/33 (57.58%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +KAYQ+QV+ N    A TL+E GY++VSGGT
Sbjct:  282 PGFKAYQQQVVKNAQAMANTLIERGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LAWIP (Serine hydroxymethyltransferase OS=Lawsonia intracellularis (strain PHE/MN1-00) GN=glyA PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.447e-18
Identity = 44/94 (46.81%), Postives = 58/94 (61.70%), Query Frame = 1 
Query:   85 NLFLVEPENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQ 363
            NL     +NHL+L++L NK+I G   EK L+   I  NKNTVP + +S  V  GIR+GTPALT+RG  E+D  KVA +   A+     I +ETQ
Sbjct:  302 NLVSGGTDNHLLLIDLTNKKITGKDAEKALDQAGITVNKNTVPFETLSPFVTSGIRIGTPALTTRGLCEQDMIKVANWIVTALN---NINNETQ 392

HSP 2 Score: 35.039 bits (79), Expect = 3.447e-18
Identity = 15/31 (48.39%), Postives = 21/31 (67.74%), Query Frame = 3 
Query:   12 YKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +  YQ+QV+ N    A+ LL+ G+ LVSGGT
Sbjct:  278 FVTYQKQVILNAKTLAKQLLDAGFNLVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOD6 (Serine hydroxymethyltransferase OS=Clostridium difficile (strain 630) GN=glyA PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 4.482e-18
Identity = 40/86 (46.51%), Postives = 56/86 (65.12%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVS-AMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSET 360
            +NHL+L++L NK I G   EK L+  +I ANKNT+P D + A+V  GIR+GTPA+T+RG  EED A +A   D  +    + K+ T
Sbjct:  310 DNHLILLDLTNKNITGKAAEKRLDDAYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESKART 395

HSP 2 Score: 34.2686 bits (77), Expect = 4.482e-18
Identity = 14/32 (43.75%), Postives = 21/32 (65.62%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            E+K YQ QV  N    A+ L++  ++L+SGGT
Sbjct:  278 EFKEYQVQVAKNAKALAEELIKRDFKLISGGT 309
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_RALPJ (Serine hydroxymethyltransferase OS=Ralstonia pickettii (strain 12J) GN=glyA PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 4.482e-18
Identity = 33/70 (47.14%), Postives = 47/70 (67.14%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAY 312
            E+H++LV+L+ K+I G   EKVL   HI  NKN +P D     V  G+R+G+PA+T+RGF E +  KVA+
Sbjct:  313 ESHVMLVDLRAKKITGKEAEKVLGDAHITVNKNAIPNDPEKPFVTSGVRLGSPAMTTRGFKEAEAVKVAH 382

HSP 2 Score: 41.9726 bits (97), Expect = 4.482e-18
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +PE+KAYQEQV+ N +  A+TL+  G  +VSG T
Sbjct:  279 SPEFKAYQEQVVKNAAAMAETLMARGLRIVSGRT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DICT6 (Serine hydroxymethyltransferase OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) GN=glyA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 4.482e-18
Identity = 41/93 (44.09%), Postives = 53/93 (56.99%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDVS-AMVPGGIRMGTPALTSRGFVEEDFAKVA--------YFFDAAVKLAVKIKSE 357
            +NHL+LV+L++K I G   EK LE   I  NKN +P D     V  GIR+GTPALT+RG  E++   VA         F D+ VK  VK + E
Sbjct:  309 DNHLMLVDLRDKGITGKEAEKALEEAGITVNKNAIPFDPQPPTVTSGIRIGTPALTTRGMKEDEMRYVARLIHEVLSNFKDSKVKEKVKKEVE 401

HSP 2 Score: 45.0542 bits (105), Expect = 4.482e-18
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+K YQ+Q++ N    A+ L+ +GY LVSGGT
Sbjct:  275 TPEFKEYQKQIILNAKAMAEELMRLGYRLVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DESHY (Serine hydroxymethyltransferase OS=Desulfitobacterium hafniense (strain Y51) GN=glyA PE=3 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 7.575e-18
Identity = 41/96 (42.71%), Postives = 59/96 (61.46%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVA 390
            +NHL+LV++++K + G   E +L+ V I  NKNT+P D  S MV  GIR+GTPA+TSRG       K+A    AA+ +A+   +E    K +D VA
Sbjct:  313 DNHLMLVDVRSKGLTGKEAEYILDEVGITVNKNTIPYDPASPMVTSGIRIGTPAVTSRGMDTLAMKKIA----AAIDIALSEPNEAGAAKARDMVA 404

HSP 2 Score: 37.3502 bits (85), Expect = 7.575e-18
Identity = 14/33 (42.42%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+  YQ++++ N    ++TL E G+ +VSGGT
Sbjct:  280 PEFVEYQKRIVENAKVLSETLAEKGFRIVSGGT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DESHD (Serine hydroxymethyltransferase OS=Desulfitobacterium hafniense (strain DCB-2 / DSM 10664) GN=glyA PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 7.575e-18
Identity = 41/96 (42.71%), Postives = 59/96 (61.46%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVA 390
            +NHL+LV++++K + G   E +L+ V I  NKNT+P D  S MV  GIR+GTPA+TSRG       K+A    AA+ +A+   +E    K +D VA
Sbjct:  313 DNHLMLVDVRSKGLTGKEAEYILDEVGITVNKNTIPYDPASPMVTSGIRIGTPAVTSRGMDTLAMKKIA----AAIDIALSEPNEAGAAKARDMVA 404

HSP 2 Score: 37.3502 bits (85), Expect = 7.575e-18
Identity = 14/33 (42.42%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+  YQ++++ N    ++TL E G+ +VSGGT
Sbjct:  280 PEFVEYQKRIVENAKVLSETLAEKGFRIVSGGT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SYMTH (Serine hydroxymethyltransferase OS=Symbiobacterium thermophilum GN=glyA PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 7.578e-18
Identity = 34/76 (44.74%), Postives = 48/76 (63.16%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAV 330
            +NHL+LV+L+ K I G   E +L+ V I  NKN +P D    MV  GIR+GTPA+T+RG  E +  ++A   D A+
Sbjct:  309 DNHLMLVDLRPKGITGRDAEHLLDRVSITVNKNAIPNDPEKPMVTSGIRIGTPAMTTRGMKEAEMVQIADLIDRAI 384

HSP 2 Score: 42.743 bits (99), Expect = 7.578e-18
Identity = 19/33 (57.58%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P+Y+AY  QV+ N    AQ LLE GY LV+GGT
Sbjct:  276 PDYRAYCGQVVKNAKALAQALLERGYRLVTGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ACIAC (Serine hydroxymethyltransferase OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=glyA PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.281e-17
Identity = 32/64 (50.00%), Postives = 43/64 (67.19%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEED 294
            E+H++LV+L+ K I G   E  L   HI  NKN++P D    MV  GIR+GTPA+T+RGF EE+
Sbjct:  312 ESHVMLVDLRAKGITGKAAEAALGQAHITINKNSIPNDPEKPMVTSGIRVGTPAITTRGFKEEE 375

HSP 2 Score: 45.0542 bits (105), Expect = 1.281e-17
Identity = 19/34 (55.88%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+KAYQ+QV  N   FA+TL+E G  ++SG T
Sbjct:  278 TPEFKAYQQQVAKNAKVFAETLIERGLRIISGRT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_COXBU (Serine hydroxymethyltransferase OS=Coxiella burnetii GN=glyA PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.664e-17
Identity = 33/71 (46.48%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            +NHL LV+L +K I G   E  L   +I  NKNTVPG+  S  V  G+R+GTPA+T+RGF E++ +++A++
Sbjct:  314 QNHLFLVSLLDKNISGKEAEAALGRANITVNKNTVPGETRSPFVTSGLRIGTPAITTRGFKEKEASQLAHW 384

HSP 2 Score: 40.817 bits (94), Expect = 1.664e-17
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y +Q+L N    A+ + E GY +VSGGT
Sbjct:  281 PEFKTYAQQILKNAKAMAEVMKERGYTIVSGGT 313
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_STRMK3.446e-1846.38Serine hydroxymethyltransferase OS=Stenotrophomona... [more]
GLYA_LAWIP3.447e-1846.81Serine hydroxymethyltransferase OS=Lawsonia intrac... [more]
GLYA_CLOD64.482e-1846.51Serine hydroxymethyltransferase OS=Clostridium dif... [more]
GLYA_RALPJ4.482e-1847.14Serine hydroxymethyltransferase OS=Ralstonia picke... [more]
GLYA_DICT64.482e-1844.09Serine hydroxymethyltransferase OS=Dictyoglomus th... [more]
GLYA_DESHY7.575e-1842.71Serine hydroxymethyltransferase OS=Desulfitobacter... [more]
GLYA_DESHD7.575e-1842.71Serine hydroxymethyltransferase OS=Desulfitobacter... [more]
GLYA_SYMTH7.578e-1844.74Serine hydroxymethyltransferase OS=Symbiobacterium... [more]
GLYA_ACIAC1.281e-1750.00Serine hydroxymethyltransferase OS=Acidovorax aven... [more]
GLYA_COXBU1.664e-1746.48Serine hydroxymethyltransferase OS=Coxiella burnet... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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