BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig809 contig Csv1_Contig809:454..857. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_COXBR (Serine hydroxymethyltransferase OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=glyA PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.664e-17
Identity = 33/71 (46.48%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            +NHL LV+L +K I G   E  L   +I  NKNTVPG+  S  V  G+R+GTPA+T+RGF E++ +++A++
Sbjct:  314 QNHLFLVSLLDKNISGKEAEAALGRANITVNKNTVPGETRSPFVTSGLRIGTPAITTRGFKEKEASQLAHW 384

HSP 2 Score: 40.817 bits (94), Expect = 1.664e-17
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y +Q+L N    A+ + E GY +VSGGT
Sbjct:  281 PEFKTYAQQILKNAKAMAEVMKERGYTIVSGGT 313
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_COXBN (Serine hydroxymethyltransferase OS=Coxiella burnetii (strain Dugway 5J108-111) GN=glyA PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.664e-17
Identity = 33/71 (46.48%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            +NHL LV+L +K I G   E  L   +I  NKNTVPG+  S  V  G+R+GTPA+T+RGF E++ +++A++
Sbjct:  314 QNHLFLVSLLDKNISGKEAEAALGRANITVNKNTVPGETRSPFVTSGLRIGTPAITTRGFKEKEASQLAHW 384

HSP 2 Score: 40.817 bits (94), Expect = 1.664e-17
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y +Q+L N    A+ + E GY +VSGGT
Sbjct:  281 PEFKTYAQQILKNAKAMAEVMKERGYTIVSGGT 313
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_COXB1 (Serine hydroxymethyltransferase OS=Coxiella burnetii (strain CbuK_Q154) GN=glyA PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.664e-17
Identity = 33/71 (46.48%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            +NHL LV+L +K I G   E  L   +I  NKNTVPG+  S  V  G+R+GTPA+T+RGF E++ +++A++
Sbjct:  314 QNHLFLVSLLDKNISGKEAEAALGRANITVNKNTVPGETRSPFVTSGLRIGTPAITTRGFKEKEASQLAHW 384

HSP 2 Score: 40.817 bits (94), Expect = 1.664e-17
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y +Q+L N    A+ + E GY +VSGGT
Sbjct:  281 PEFKTYAQQILKNAKAMAEVMKERGYTIVSGGT 313
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_STRM5 (Serine hydroxymethyltransferase OS=Stenotrophomonas maltophilia (strain R551-3) GN=glyA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.665e-17
Identity = 32/69 (46.38%), Postives = 45/69 (65.22%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV++  K + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+VE+D   +A
Sbjct:  315 QNHLMLVDMIGKDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAVTTRGYVEQDCVDLA 383

HSP 2 Score: 43.1282 bits (100), Expect = 1.665e-17
Identity = 18/33 (54.55%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +KAYQ+QV+ N    A TL+  GY++VSGGT
Sbjct:  282 PGFKAYQQQVVKNAQAMANTLIARGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CLOTH (Serine hydroxymethyltransferase OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237) GN=glyA PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.665e-17
Identity = 34/69 (49.28%), Postives = 48/69 (69.57%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHL+LV+L+NK + G  V+ +L+ V I  NKN +P D  S  V  GIR+GTPA+T+RG  EED  ++A
Sbjct:  312 DNHLMLVDLRNKGLTGKYVQHILDEVCITVNKNGIPFDPESPFVTSGIRIGTPAVTARGMKEEDMVEIA 380

HSP 2 Score: 40.817 bits (94), Expect = 1.665e-17
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+K YQ+Q++ N    A  L+E G +LVSGGT
Sbjct:  278 TDEFKQYQQQIVKNAKTLANALMEKGIDLVSGGT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_SALRD (Serine hydroxymethyltransferase OS=Salinibacter ruber (strain DSM 13855) GN=glyA PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.162e-17
Identity = 38/77 (49.35%), Postives = 52/77 (67.53%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVP-GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHLVL++L+NK + G   E+ LEA  I ANKN VP  D S  V  G+R+GTPA+T+RGF  ++FA VA   D  ++
Sbjct:  326 DNHLVLIDLRNKGLTGKEAEQALEAAGITANKNMVPFDDKSPFVTSGLRLGTPAMTTRGFGPDEFAHVAEMIDRVLQ 402

HSP 2 Score: 32.3426 bits (72), Expect = 2.162e-17
Identity = 14/33 (42.42%), Postives = 18/33 (54.55%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +  Y +QV+ N       L E GY+LVS GT
Sbjct:  293 PSFAEYTQQVVDNAQAMGAELRERGYDLVSDGT 325
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_COXB2 (Serine hydroxymethyltransferase OS=Coxiella burnetii (strain CbuG_Q212) GN=glyA PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.813e-17
Identity = 34/71 (47.89%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYF 315
            +NHL LV+L NK I G   E  L   +I  NKNTVPG+  S  V  G+R+GTPA+T+RGF E++ +++A++
Sbjct:  314 QNHLFLVSLLNKNISGKEAEAALGRANITVNKNTVPGETRSPFVTSGLRIGTPAITTRGFKEKEASQLAHW 384

HSP 2 Score: 38.1206 bits (87), Expect = 2.813e-17
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y +Q+L N    A+ + E  Y +VSGGT
Sbjct:  281 PEFKTYAQQILKNAKAMAEVMKERDYTIVSGGT 313
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_XANCP (Serine hydroxymethyltransferase OS=Xanthomonas campestris pv. campestris GN=glyA PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.814e-17
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ENHL+LV++  + + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+ E+D   +A
Sbjct:  315 ENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDCVDLA 383

HSP 2 Score: 44.2838 bits (103), Expect = 2.814e-17
Identity = 18/33 (54.55%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ+QV+ N    A TL+  GY++VSGGT
Sbjct:  282 PEFKTYQQQVVKNAQAMANTLIARGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_XANCB (Serine hydroxymethyltransferase OS=Xanthomonas campestris pv. campestris (strain B100) GN=glyA PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.814e-17
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ENHL+LV++  + + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+ E+D   +A
Sbjct:  315 ENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDCVDLA 383

HSP 2 Score: 44.2838 bits (103), Expect = 2.814e-17
Identity = 18/33 (54.55%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ+QV+ N    A TL+  GY++VSGGT
Sbjct:  282 PEFKTYQQQVVKNAQAMANTLIARGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_XANC8 (Serine hydroxymethyltransferase OS=Xanthomonas campestris pv. campestris (strain 8004) GN=glyA PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.814e-17
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ENHL+LV++  + + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+ E+D   +A
Sbjct:  315 ENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDCVDLA 383

HSP 2 Score: 44.2838 bits (103), Expect = 2.814e-17
Identity = 18/33 (54.55%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K YQ+QV+ N    A TL+  GY++VSGGT
Sbjct:  282 PEFKTYQQQVVKNAQAMANTLIARGYKIVSGGT 314
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_COXBR1.664e-1746.48Serine hydroxymethyltransferase OS=Coxiella burnet... [more]
GLYA_COXBN1.664e-1746.48Serine hydroxymethyltransferase OS=Coxiella burnet... [more]
GLYA_COXB11.664e-1746.48Serine hydroxymethyltransferase OS=Coxiella burnet... [more]
GLYA_STRM51.665e-1746.38Serine hydroxymethyltransferase OS=Stenotrophomona... [more]
GLYA_CLOTH1.665e-1749.28Serine hydroxymethyltransferase OS=Clostridium the... [more]
GLYA_SALRD2.162e-1749.35Serine hydroxymethyltransferase OS=Salinibacter ru... [more]
GLYA_COXB22.813e-1747.89Serine hydroxymethyltransferase OS=Coxiella burnet... [more]
GLYA_XANCP2.814e-1744.93Serine hydroxymethyltransferase OS=Xanthomonas cam... [more]
GLYA_XANCB2.814e-1744.93Serine hydroxymethyltransferase OS=Xanthomonas cam... [more]
GLYA_XANC82.814e-1744.93Serine hydroxymethyltransferase OS=Xanthomonas cam... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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