BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig809 contig Csv1_Contig809:454..857. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ALKMQ (Serine hydroxymethyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=glyA PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.038e-17
Identity = 37/87 (42.53%), Postives = 54/87 (62.07%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQ 363
            + HL+L++L+NK I G   E++ + V I  NKNT+P D  S  V  GIR+GTPA+T+RG  EE+  K+A   +  +    K+ SE Q
Sbjct:  311 DTHLLLIDLRNKNITGKDAERLFDEVGITVNKNTIPFDPQSPFVTSGIRIGTPAVTTRGMKEEEMKKIAGVMNIIIDHPEKV-SEAQ 396

HSP 2 Score: 38.1206 bits (87), Expect = 8.038e-17
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +KAYQEQV+ N     + L + G++LVS GT
Sbjct:  278 PGFKAYQEQVIKNAKALGEELKKQGFDLVSDGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_STACT (Serine hydroxymethyltransferase OS=Staphylococcus carnosus (strain TM300) GN=glyA PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 8.038e-17
Identity = 41/101 (40.59%), Postives = 54/101 (53.47%), Query Frame = 1 
Query:  106 ENHLVLVNLKNK-RIDGSRVEKVLEAVHIAANKNTVPGDVSA-MVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKDFVATTQS 402
            +NHLV V++K    I G   E+ L+ V I  NKNT+P D     V  G+R+GTPA T+RGF E DF +VA      V+     K E +  +  D VA   S
Sbjct:  309 DNHLVAVDVKGSFGITGKEAEEALDEVGITCNKNTIPFDQEKPFVTSGLRLGTPAATTRGFEEADFEEVAKIISLVVQ---NPKDEAKLKEASDRVAALTS 406

HSP 2 Score: 43.1282 bits (100), Expect = 8.038e-17
Identity = 18/33 (54.55%), Postives = 24/33 (72.73%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P++K YQEQV+ N    A TL+E G+ +VSGGT
Sbjct:  276 PDFKDYQEQVVKNAKALADTLIEEGFRVVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_XYLFT (Serine hydroxymethyltransferase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=glyA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.045e-16
Identity = 39/98 (39.80%), Postives = 54/98 (55.10%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFF--------DAAVKLAVKIKSETQGTK 372
            ENHL+LV++  + + G   E  L  VHI  NKN VP D  S  V  G+R+GTPA+T+RG+ E+D   +A++         D  V  AV+ K   Q  K
Sbjct:  315 ENHLMLVDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTSGLRLGTPAVTTRGYQEQDCVDLAHWIADVLDAPADVTVIAAVREKVAAQCKK 412

HSP 2 Score: 40.4318 bits (93), Expect = 1.045e-16
Identity = 17/33 (51.52%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ+QV+ N    A TL+  GY++VSGGT
Sbjct:  282 PAFKVYQQQVVKNAKAMAGTLMLRGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_XYLF2 (Serine hydroxymethyltransferase OS=Xylella fastidiosa (strain M23) GN=glyA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.045e-16
Identity = 39/98 (39.80%), Postives = 54/98 (55.10%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFF--------DAAVKLAVKIKSETQGTK 372
            ENHL+LV++  + + G   E  L  VHI  NKN VP D  S  V  G+R+GTPA+T+RG+ E+D   +A++         D  V  AV+ K   Q  K
Sbjct:  315 ENHLMLVDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTSGLRLGTPAVTTRGYQEQDCVDLAHWIADVLDAPADVTVIAAVREKVAAQCKK 412

HSP 2 Score: 40.4318 bits (93), Expect = 1.045e-16
Identity = 17/33 (51.52%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ+QV+ N    A TL+  GY++VSGGT
Sbjct:  282 PAFKVYQQQVVKNAKAMAGTLMLRGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_STAHJ (Serine hydroxymethyltransferase OS=Staphylococcus haemolyticus (strain JCSC1435) GN=glyA PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.045e-16
Identity = 37/94 (39.36%), Postives = 55/94 (58.51%), Query Frame = 1 
Query:  106 ENHLVLVNLKNK-RIDGSRVEKVLEAVHIAANKNTVPGDVS-AMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVKIKSETQGTKLKD 381
            +NHL+ V++KN   + G   E  L+++ I  NKNT+P D   A V  GIR+GTP  T+RGF EE F +V         +++ +K+    TKLK+
Sbjct:  309 DNHLLSVDVKNSVNVTGKEAEATLDSIGITCNKNTIPFDQEKAFVTSGIRLGTPTATTRGFDEEAFKEVGRI------ISLALKNPNNDTKLKE 396

HSP 2 Score: 40.817 bits (94), Expect = 1.045e-16
Identity = 17/32 (53.12%), Postives = 23/32 (71.88%), Query Frame = 3 
Query:    9 EYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            ++K YQEQV+ N    +QTL E G+ +VSGGT
Sbjct:  277 DFKVYQEQVIKNAKVLSQTLQEEGFRIVSGGT 308
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CHLCH (Serine hydroxymethyltransferase OS=Chlorobium chlorochromatii (strain CaD3) GN=glyA PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.356e-16
Identity = 34/81 (41.98%), Postives = 48/81 (59.26%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVP-GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVKLAVK 345
            +NHL+L++L+NK + G   E +L    I  NKN VP  D S  V  GIR+GTPA+T+RG  E +  ++A   D  +  A K
Sbjct:  330 KNHLMLLDLRNKNVTGKEAENLLHEAGITVNKNMVPFDDKSPFVTSGIRIGTPAMTTRGMKEAESRRIAELIDQVITSASK 410

HSP 2 Score: 38.891 bits (89), Expect = 1.356e-16
Identity = 16/33 (48.48%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K Y  QV+ N S  A   +E+GY +VSGGT
Sbjct:  297 PAFKEYAAQVMKNASTMASRFMELGYTIVSGGT 329
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CELJU (Serine hydroxymethyltransferase OS=Cellvibrio japonicus (strain Ueda107) GN=glyA PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.358e-16
Identity = 32/64 (50.00%), Postives = 40/64 (62.50%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEED 294
            ENHL+LV+L  K   G   ++ L   HI  NKN VP D  S  V  GIR+GTPA+T+RGF E +
Sbjct:  315 ENHLMLVDLIGKPYSGKDADEALGKAHITVNKNAVPNDPRSPFVTSGIRVGTPAITTRGFKEAE 378

HSP 2 Score: 44.2838 bits (103), Expect = 1.358e-16
Identity = 19/34 (55.88%), Postives = 24/34 (70.59%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPEYK YQ+QV+ N    A T +E G ++VSGGT
Sbjct:  281 TPEYKDYQQQVVKNAKAMAATFIERGIKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_LYSSC (Serine hydroxymethyltransferase OS=Lysinibacillus sphaericus (strain C3-41) GN=glyA PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.358e-16
Identity = 37/77 (48.05%), Postives = 49/77 (63.64%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL+L+N+K+  + G   E  L+ V I  NKNT+P D  S  V  GIR+GTPA+TSRGF EED  +V     A +K
Sbjct:  311 DNHLLLLNVKSLGLTGKVAEHALDEVGITTNKNTIPYDTESPFVTSGIRIGTPAVTSRGFKEEDMKEVGAIIAAVLK 387

HSP 2 Score: 35.4242 bits (80), Expect = 1.358e-16
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE+K Y +Q+  N    A+ L+  G E+VSGGT
Sbjct:  278 PEFKDYAKQIKLNAKALAEVLIAEGVEIVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_AYWBP (Serine hydroxymethyltransferase OS=Aster yellows witches'-broom phytoplasma (strain AYWB) GN=glyA PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.764e-16
Identity = 34/78 (43.59%), Postives = 51/78 (65.38%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRID--GSRVEKVLEAVHIAANKNTVPGDVSA-MVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAV 330
            +NHL L+++K+K  +  G+++  +LE ++I  NKNT+P D     V  GIR+GTPA+T+ GF E DF  VA   D A+
Sbjct:  327 DNHLFLIDVKHKNTEFTGAKIANMLEKINIVVNKNTIPFDQEKPFVTSGIRIGTPAMTTVGFRENDFVAVADLMDKAI 404

HSP 2 Score: 38.5058 bits (88), Expect = 1.764e-16
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ+QV+ N + FA+   + GY +VSG T
Sbjct:  294 PSFKEYQKQVIKNANTFAKAFQQKGYRIVSGST 326
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_THEP1 (Serine hydroxymethyltransferase OS=Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) GN=glyA PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.764e-16
Identity = 34/73 (46.58%), Postives = 45/73 (61.64%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFD 321
            + HL LV+L  K I G   EK LE+  I  NKNT+P +  S  V  GIR+GTPA+T+RG  EE+  ++A   D
Sbjct:  311 DTHLFLVDLTPKDITGKAAEKALESCGITVNKNTIPNEKRSPFVASGIRIGTPAVTTRGMKEEEMEEIAEMID 383

HSP 2 Score: 40.4318 bits (93), Expect = 1.764e-16
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+K YQ+QV+ N  K A+   + GY +VSGGT
Sbjct:  277 TEEFKEYQKQVVKNAKKMAEEFQKRGYRIVSGGT 310
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_ALKMQ8.038e-1742.53Serine hydroxymethyltransferase OS=Alkaliphilus me... [more]
GLYA_STACT8.038e-1740.59Serine hydroxymethyltransferase OS=Staphylococcus ... [more]
GLYA_XYLFT1.045e-1639.80Serine hydroxymethyltransferase OS=Xylella fastidi... [more]
GLYA_XYLF21.045e-1639.80Serine hydroxymethyltransferase OS=Xylella fastidi... [more]
GLYA_STAHJ1.045e-1639.36Serine hydroxymethyltransferase OS=Staphylococcus ... [more]
GLYA_CHLCH1.356e-1641.98Serine hydroxymethyltransferase OS=Chlorobium chlo... [more]
GLYA_CELJU1.358e-1650.00Serine hydroxymethyltransferase OS=Cellvibrio japo... [more]
GLYA_LYSSC1.358e-1648.05Serine hydroxymethyltransferase OS=Lysinibacillus ... [more]
GLYA_AYWBP1.764e-1643.59Serine hydroxymethyltransferase OS=Aster yellows w... [more]
GLYA_THEP11.764e-1646.58Serine hydroxymethyltransferase OS=Thermotoga petr... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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