BQ623771

Overview
NameBQ623771
Unique NameBQ623771
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length405
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_THEMA (Serine hydroxymethyltransferase OS=Thermotoga maritima GN=glyA PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.764e-16
Identity = 34/73 (46.58%), Postives = 45/73 (61.64%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFD 321
            + HL LV+L  K I G   EK LE+  I  NKNT+P +  S  V  GIR+GTPA+T+RG  EE+  ++A   D
Sbjct:  311 DTHLFLVDLTPKDITGKAAEKALESCGITVNKNTIPNEKRSPFVASGIRIGTPAVTTRGMKEEEMEEIAEMID 383

HSP 2 Score: 40.4318 bits (93), Expect = 1.764e-16
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+K YQ+QV+ N  K A+   + GY +VSGGT
Sbjct:  277 TEEFKEYQKQVVKNAKKMAEEFQKRGYRIVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_RICM5 (Serine hydroxymethyltransferase OS=Rickettsia massiliae (strain Mtu5) GN=glyA PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.765e-16
Identity = 32/70 (45.71%), Postives = 42/70 (60.00%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEEDFAKVAY 312
            +NH+VLV+L+   I G      L+   I  NKN +P D  S  +  GIR+GTPA T+RGF E+DF  V Y
Sbjct:  314 DNHIVLVDLRKDGITGKFAANSLDRAGITCNKNAIPFDETSPFITSGIRLGTPACTTRGFKEKDFVLVGY 383

HSP 2 Score: 42.3578 bits (98), Expect = 1.765e-16
Identity = 17/33 (51.52%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PEYK+Y +QV+SN    A +L E GY++++GGT
Sbjct:  281 PEYKSYIQQVISNAKALASSLQERGYDILTGGT 313
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_XANC5 (Serine hydroxymethyltransferase OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=glyA PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.765e-16
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ENHL+LV++  + + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+ E+D   +A
Sbjct:  315 ENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDSIDLA 383

HSP 2 Score: 41.9726 bits (97), Expect = 1.765e-16
Identity = 17/33 (51.52%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ+QV+ N    A TL+  GY++VSGGT
Sbjct:  282 PAFKTYQQQVVKNAQAMANTLIARGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_XANAC (Serine hydroxymethyltransferase OS=Xanthomonas axonopodis pv. citri GN=glyA PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.765e-16
Identity = 31/69 (44.93%), Postives = 44/69 (63.77%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            ENHL+LV++  + + G   E  L   HI  NKN+VP D  S  V  G+R+GTPA+T+RG+ E+D   +A
Sbjct:  315 ENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDSIDLA 383

HSP 2 Score: 41.9726 bits (97), Expect = 1.765e-16
Identity = 17/33 (51.52%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ+QV+ N    A TL+  GY++VSGGT
Sbjct:  282 PAFKTYQQQVVKNAQAMANTLIARGYKIVSGGT 314
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_CUPTR (Serine hydroxymethyltransferase OS=Cupriavidus taiwanensis (strain R1 / LMG 19424) GN=glyA PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.765e-16
Identity = 29/64 (45.31%), Postives = 43/64 (67.19%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEED 294
            E+H++LV+L+ K I G   E++L   H+  NKN +P D     V  GIR+G+PA+T+RGF EE+
Sbjct:  313 ESHVMLVDLRAKNITGKEAERILGEAHLTVNKNAIPNDPEKPFVTSGIRVGSPAMTTRGFKEEE 376

HSP 2 Score: 41.9726 bits (97), Expect = 1.765e-16
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            TPE+KAYQ+QV+ N +  A+TL+  G  +VSG T
Sbjct:  279 TPEFKAYQQQVVKNAAVLAETLIARGLRIVSGRT 312
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_THESQ (Serine hydroxymethyltransferase OS=Thermotoga sp. (strain RQ2) GN=glyA PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.293e-16
Identity = 34/73 (46.58%), Postives = 45/73 (61.64%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFD 321
            + HL LV+L  K I G   EK LE+  I  NKNT+P +  S  V  GIR+GTPA+T+RG  EE+  ++A   D
Sbjct:  311 DTHLFLVDLTPKDITGKAAEKALESCGITVNKNTIPNEKRSPFVASGIRIGTPAVTTRGMKEEEMEEIAEMID 383

HSP 2 Score: 40.0466 bits (92), Expect = 2.293e-16
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            T E+K YQ QV+ N  K A+   + GY +VSGGT
Sbjct:  277 TEEFKEYQNQVVKNAKKMAEEFQKRGYRIVSGGT 310
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_MYCPU (Serine hydroxymethyltransferase OS=Mycoplasma pulmonis GN=glyA PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.294e-16
Identity = 35/78 (44.87%), Postives = 51/78 (65.38%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKR-IDGSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDAAVK 333
            +NHL++VN+ +   I G + E +L  ++I  NKNT+P D  S MV  GIR+GTPA+T+RGF E +F  +A     A+K
Sbjct:  310 DNHLLIVNVFDSYGITGKKAENILGKINITVNKNTIPFDTNSPMVTSGIRLGTPAMTTRGFKENEFILIARIMVKALK 387

HSP 2 Score: 38.1206 bits (87), Expect = 2.294e-16
Identity = 14/33 (42.42%), Postives = 23/33 (69.70%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            P +K YQ+QV+ N   FA   +++G +++SGGT
Sbjct:  277 PSFKTYQDQVVKNAKVFADEFIKLGAKVISGGT 309
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_DIAST (Serine hydroxymethyltransferase OS=Diaphorobacter sp. (strain TPSY) GN=glyA PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.294e-16
Identity = 32/64 (50.00%), Postives = 42/64 (65.62%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEED 294
            E+H++LV+L+ K I G   E  L   HI  NKN +P D    MV  GIR+GTPA+T+RGF EE+
Sbjct:  312 ESHVMLVDLRAKGITGKEAEAALGKAHITINKNAIPNDPEKPMVTSGIRVGTPAITTRGFKEEE 375

HSP 2 Score: 40.817 bits (94), Expect = 2.294e-16
Identity = 17/34 (50.00%), Postives = 24/34 (70.59%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +PE+K YQ+QV  N   FA+TL++ G  +VSG T
Sbjct:  278 SPEFKQYQQQVTKNAKVFAETLIQRGLRIVSGRT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_ACISJ (Serine hydroxymethyltransferase OS=Acidovorax sp. (strain JS42) GN=glyA PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.294e-16
Identity = 32/64 (50.00%), Postives = 42/64 (65.62%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRIDGSRVEKVLEAVHIAANKNTVPGD-VSAMVPGGIRMGTPALTSRGFVEED 294
            E+H++LV+L+ K I G   E  L   HI  NKN +P D    MV  GIR+GTPA+T+RGF EE+
Sbjct:  312 ESHVMLVDLRAKGITGKEAEAALGKAHITINKNAIPNDPEKPMVTSGIRVGTPAITTRGFKEEE 375

HSP 2 Score: 40.817 bits (94), Expect = 2.294e-16
Identity = 17/34 (50.00%), Postives = 24/34 (70.59%), Query Frame = 3 
Query:    3 TPEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            +PE+K YQ+QV  N   FA+TL++ G  +VSG T
Sbjct:  278 SPEFKQYQQQVTKNAKVFAETLIQRGLRIVSGRT 311
[Close]
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Match: GLYA_NATPD (Serine hydroxymethyltransferase OS=Natronomonas pharaonis (strain DSM 2160 / ATCC 35678) GN=glyA PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.981e-16
Identity = 39/71 (54.93%), Postives = 48/71 (67.61%), Query Frame = 1 
Query:  106 ENHLVLVNLKNKRID--GSRVEKVLEAVHIAANKNTVPGDV-SAMVPGGIRMGTPALTSRGFVEEDFAKVA 309
            +NHLVLV+L+    D  G+  E+ LEA  I  NKNTVPG+  SA  P GIR GTPALT+RGF E+   +VA
Sbjct:  311 DNHLVLVDLRESHPDTSGTVAEEALEAAGIVLNKNTVPGETRSAFNPSGIRAGTPALTTRGFDEQACERVA 381

HSP 2 Score: 36.5798 bits (83), Expect = 2.981e-16
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:    6 PEYKAYQEQVLSNCSKFAQTLLEMGYELVSGGT 104
            PE++ Y +QV+ N     + L E G+ LVSGGT
Sbjct:  278 PEFEQYAQQVIDNAEALGERLQEHGFSLVSGGT 310
The following BLAST results are available for this feature:
BLAST of BQ623771 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_THEMA1.764e-1646.58Serine hydroxymethyltransferase OS=Thermotoga mari... [more]
GLYA_RICM51.765e-1645.71Serine hydroxymethyltransferase OS=Rickettsia mass... [more]
GLYA_XANC51.765e-1644.93Serine hydroxymethyltransferase OS=Xanthomonas cam... [more]
GLYA_XANAC1.765e-1644.93Serine hydroxymethyltransferase OS=Xanthomonas axo... [more]
GLYA_CUPTR1.765e-1645.31Serine hydroxymethyltransferase OS=Cupriavidus tai... [more]
GLYA_THESQ2.293e-1646.58Serine hydroxymethyltransferase OS=Thermotoga sp. ... [more]
GLYA_MYCPU2.294e-1644.87Serine hydroxymethyltransferase OS=Mycoplasma pulm... [more]
GLYA_DIAST2.294e-1650.00Serine hydroxymethyltransferase OS=Diaphorobacter ... [more]
GLYA_ACISJ2.294e-1650.00Serine hydroxymethyltransferase OS=Acidovorax sp. ... [more]
GLYA_NATPD2.981e-1654.93Serine hydroxymethyltransferase OS=Natronomonas ph... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00862 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00862 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623771 ID=BQ623771; Name=BQ623771; organism=Citrus sinensis; type=EST; length=405bp
CTACTCCAGAGTATAAAGCCTATCAAGAGCAAGTTCTGAGCAATTGCTCG
AAATTTGCACAGACTCTGCTCGAGATGGGCTATGAACTTGTTTCTGGTGG
AACCTGAGAACCATCTAGTGTTGGTGAATTTGAAAAACAAGAGAATTGAT
GGTTCCAGAGTTGAAAAAGTGTTGGAAGCTGTTCATATTGCAGCCAACAA
AAACACTGTTCCTGGGGATGTGTCAGCCATGGTTCCAGGTGGCATCAGGA
TGGGAACTCCGGCTCTTACTTCTAGGGGATTTGTTGAGGAGGATTTTGCC
AAGGTAGCATATTTCTTTGATGCTGCTGTGAAGTTGGCTGTGAAGATCAA
GTCTGAAACTCAAGGAACAAAGTTGAAAGATTTCGTGGCCACAACACAGT
CCGCT
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