BQ623832

Overview
NameBQ623832
Unique NameBQ623832
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length580
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2225 contig Csv1_Contig2225:39..616. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UBC4_SCHPO (Ubiquitin-conjugating enzyme E2 4 OS=Schizosaccharomyces pombe GN=ubc4 PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 2.911e-58
Identity = 97/118 (82.20%), Postives = 109/118 (92.37%), Query Frame = 3 
Query:  168 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            MA KRI +EL DL KDPP+SCSAGPV +D+FHWQATIMGP+DSPYAGGVF ++IHFP DYPFKPPKV F T+++HPNINSNGSICLDIL++QWSPALTISKVLLSICSLLTDPNPDDP
Sbjct:    1 MALKRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDP 118

HSP 2 Score: 30.4166 bits (67), Expect = 2.911e-58
Identity = 11/17 (64.71%), Postives = 16/17 (94.12%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQYE 574
            L PEIAH+YK+D+++YE
Sbjct:  119 LVPEIAHVYKTDRSRYE 135
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UBC4_YEAST (Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae GN=UBC4 PE=1 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 4.946e-58
Identity = 92/117 (78.63%), Postives = 110/117 (94.02%), Query Frame = 3 
Query:  171 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            +SKRI KEL DL++DPPTSCSAGPV +D++HWQA+IMGP+DSPYAGGVF ++IHFP DYPFKPPK++F TK++HPNIN+NG+ICLDILK+QWSPALT+SKVLLSICSLLTD NPDDP
Sbjct:    3 SSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDP 119

HSP 2 Score: 31.9574 bits (71), Expect = 4.946e-58
Identity = 12/19 (63.16%), Postives = 17/19 (89.47%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQYEST 580
            L PEIAH+YK+D+ +YE+T
Sbjct:  120 LVPEIAHIYKTDRPKYEAT 138
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UB2D4_HUMAN (Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens GN=UBE2D4 PE=1 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 1.864e-57
Identity = 97/118 (82.20%), Postives = 106/118 (89.83%), Query Frame = 3 
Query:  168 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            MA KRI KEL DLQ+DPP  CSAGPV +D+FHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF TK++HPNINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 26.9498 bits (58), Expect = 1.864e-57
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQY 571
            L PEIAH YK+D+ +Y
Sbjct:  119 LVPEIAHTYKADREKY 134
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UBC5_YEAST (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae GN=UBC5 PE=1 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 7.018e-57
Identity = 93/117 (79.49%), Postives = 107/117 (91.45%), Query Frame = 3 
Query:  171 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            +SKRI KEL DL +DPP SCSAGPV +D++HWQA+IMGPSDSPYAGGVF ++IHFP DYPFKPPKV F TK++HPNINS+G+ICLDILK+QWSPALT+SKVLLSICSLLTD NPDDP
Sbjct:    3 SSKRIAKELSDLGRDPPASCSAGPVGDDLYHWQASIMGPSDSPYAGGVFFLSIHFPTDYPFKPPKVNFTTKIYHPNINSSGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDP 119

HSP 2 Score: 31.9574 bits (71), Expect = 7.018e-57
Identity = 13/19 (68.42%), Postives = 17/19 (89.47%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQYEST 580
            L PEIA +YK+DKA+YE+T
Sbjct:  120 LVPEIAQIYKTDKAKYEAT 138
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UB2D2_XENLA (Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laevis GN=ube2d2 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 3.448e-56
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  168 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 24.6386 bits (52), Expect = 3.448e-56
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQY 571
            L PEIA +YK+D+ +Y
Sbjct:  119 LVPEIARIYKTDREKY 134
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UB2D2_RAT (Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norvegicus GN=Ube2d2 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 3.448e-56
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  168 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 24.6386 bits (52), Expect = 3.448e-56
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQY 571
            L PEIA +YK+D+ +Y
Sbjct:  119 LVPEIARIYKTDREKY 134
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UB2D2_MOUSE (Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus GN=Ube2d2 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 3.448e-56
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  168 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 24.6386 bits (52), Expect = 3.448e-56
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQY 571
            L PEIA +YK+D+ +Y
Sbjct:  119 LVPEIARIYKTDREKY 134
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UB2D2_HUMAN (Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens GN=UBE2D2 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 3.448e-56
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  168 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 24.6386 bits (52), Expect = 3.448e-56
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQY 571
            L PEIA +YK+D+ +Y
Sbjct:  119 LVPEIARIYKTDREKY 134
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UB2D2_BOVIN (Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 3.448e-56
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  168 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 24.6386 bits (52), Expect = 3.448e-56
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQY 571
            L PEIA +YK+D+ +Y
Sbjct:  119 LVPEIARIYKTDREKY 134
[Close]
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Match: UB2D3_RAT (Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norvegicus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 4.496e-56
Identity = 97/118 (82.20%), Postives = 105/118 (88.98%), Query Frame = 3 
Query:  168 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDP 521
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDP
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDP 118

HSP 2 Score: 24.2534 bits (51), Expect = 4.496e-56
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  524 LYPEIAHMYKSDKAQY 571
            L PEIA +YK+D+ +Y
Sbjct:  119 LVPEIARIYKTDRDKY 134
The following BLAST results are available for this feature:
BLAST of BQ623832 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 266
Match NameE-valueIdentityDescription
UBC4_SCHPO2.911e-5882.20Ubiquitin-conjugating enzyme E2 4 OS=Schizosacchar... [more]
UBC4_YEAST4.946e-5878.63Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces... [more]
UB2D4_HUMAN1.864e-5782.20Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens... [more]
UBC5_YEAST7.018e-5779.49Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharo... [more]
UB2D2_XENLA3.448e-5682.20Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laev... [more]
UB2D2_RAT3.448e-5682.20Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norve... [more]
UB2D2_MOUSE3.448e-5682.20Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus... [more]
UB2D2_HUMAN3.448e-5682.20Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens... [more]
UB2D2_BOVIN3.448e-5682.20Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus G... [more]
UB2D3_RAT4.496e-5682.20Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norve... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00912 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00912 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623832 ID=BQ623832; Name=BQ623832; organism=Citrus sinensis; type=EST; length=580bp
GCAAGGGGTTGATAGAGGAGCGAGGCGGGGGCGGCTTGACGACCTAGATC
TGCTTCACCGTTTCGCTTTCTCGACGCTATCGCTCTCCGATCCTGTTCTC
TGCCGCGCAAGTTTCGCAGGTCTTAAGGTTCGTTGTAGTTTCAATTTTAA
TAATCCCCTGAAGAGTCATGGCTTCAAAGCGTATTTTGAAGGAACTCAAG
GATTTGCAGAAGGATCCTCCCACGTCTTGCAGCGCTGGCCCTGTGGCTGA
AGACATGTTCCACTGGCAAGCAACAATTATGGGCCCTTCTGATAGCCCAT
ATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCCTCCTGATTATCCT
TTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCTTCCACCCGAACAT
CAACAGCAATGGAAGCATCTGTCTTGATATCCTTAAAGAACAGTGGAGTC
CAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCTGCTCCTTGCTTACT
GATCCAAATCCTGACGACCCCTCTTGTACCCGGAGATTGCCCACATGTAC
AAGTCCGACAAGGCCCAATATGAATCCACG
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