BQ623839

Overview
NameBQ623839
Unique NameBQ623839
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length612
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_FLAJ1 (Triosephosphate isomerase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) GN=tpiA PE=3 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 4.339e-39
Identity = 83/186 (44.62%), Postives = 117/186 (62.90%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLK--AYADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLL 583
            I ++AQN    +GGAFTGEIS + L  IG   V+LGHSERR +  E D  I  K   AL   + VI C GE+L++R++G  F++   QL+   +  A  SW  +V+AYEPVWAIGTG+ A+PEQAQE+H  +R+ ++    A++A +  I+YGGSV   N  E+ +K  +    +GGA+LKA   L
Sbjct:   59 IGVSAQNVHQAEGGAFTGEISADMLTSIGVNTVILGHSERRAIFHETDALIANKVDTALKHDMTVIFCFGEELKDRQSGNHFNIVENQLRDGVFHIAKESWSKIVLAYEPVWAIGTGETASPEQAQEMHEFIRETIRKAFGAEIADEVSILYGGSVKPENAKEIFSKPDVDGGLIGGAALKADDFL 244
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_PASMU (Triosephosphate isomerase OS=Pasteurella multocida GN=tpiA PE=3 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 5.668e-39
Identity = 92/182 (50.55%), Postives = 116/182 (63.74%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADAI--PSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKA 571
            I + AQN  V   GAFTG+IS E LKD G K++++GHSERR    E D+FI KK A     GL  + CIGE   E EAGKT +VC +Q+ A  + +   +++  VIAYEP+WAIGTGK ATP QAQ VHA +R  +    +A VA +  I YGGSVN +N AEL  +  +    VGGASLKA
Sbjct:   66 IALGAQNVDVNVQGAFTGDISTEMLKDFGAKYIIIGHSERRTYHKESDEFIAKKFAALKQAGLVPVLCIGESEAENEAGKTEEVCARQIDAVLNTLGAEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAKKDNA-VAEQVIIQYGGSVNDANAAELFTQPDIDGALVGGASLKA 246
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_HAEIN (Triosephosphate isomerase OS=Haemophilus influenzae GN=tpiA PE=1 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 5.668e-39
Identity = 90/182 (49.45%), Postives = 116/182 (63.74%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADA--IPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKA 571
            I++ AQN  +   GAFTG+IS E LKD G K++++GHSERR    E D+F+ KK       GL  + CIGE   E EAGKT +VC +Q+ A  +A  + +++  VIAYEP+WAIGTGK ATP QAQ VHA +R  +     A VA +  I YGGSVN +N AEL  +  +    VGGASLKA
Sbjct:   68 IQLGAQNVDINVKGAFTGDISTEMLKDFGAKYIIIGHSERRTYHKESDEFVAKKFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVINALGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQA-VAEQVIIQYGGSVNDANAAELFTQPDIDGALVGGASLKA 248
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_HAEIE (Triosephosphate isomerase OS=Haemophilus influenzae (strain PittEE) GN=tpiA PE=3 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 5.668e-39
Identity = 90/182 (49.45%), Postives = 116/182 (63.74%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADA--IPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKA 571
            I++ AQN  +   GAFTG+IS E LKD G K++++GHSERR    E D+F+ KK       GL  + CIGE   E EAGKT +VC +Q+ A  +A  + +++  VIAYEP+WAIGTGK ATP QAQ VHA +R  +     A VA +  I YGGSVN +N AEL  +  +    VGGASLKA
Sbjct:   68 IQLGAQNVDINVKGAFTGDISTEMLKDFGAKYIIIGHSERRTYHKESDEFVAKKFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVINALGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQA-VAEQVIIQYGGSVNDANAAELFTQPDIDGALVGGASLKA 248
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_SHISS (Triosephosphate isomerase OS=Shigella sonnei (strain Ss046) GN=tpiA PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 9.667e-39
Identity = 91/184 (49.46%), Postives = 115/184 (62.50%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQS 577
            I + AQN  +   GAFTGE S   LKDIG +++++GHSERR    E D+ I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  +  +    VGGASLKA +
Sbjct:   59 IMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_SHIFL (Triosephosphate isomerase OS=Shigella flexneri GN=tpiA PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 9.667e-39
Identity = 91/184 (49.46%), Postives = 115/184 (62.50%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQS 577
            I + AQN  +   GAFTGE S   LKDIG +++++GHSERR    E D+ I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  +  +    VGGASLKA +
Sbjct:   59 IMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_SHIF8 (Triosephosphate isomerase OS=Shigella flexneri serotype 5b (strain 8401) GN=tpiA PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 9.667e-39
Identity = 91/184 (49.46%), Postives = 115/184 (62.50%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQS 577
            I + AQN  +   GAFTGE S   LKDIG +++++GHSERR    E D+ I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  +  +    VGGASLKA +
Sbjct:   59 IMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_SHIDS (Triosephosphate isomerase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=tpiA PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 9.667e-39
Identity = 91/184 (49.46%), Postives = 115/184 (62.50%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQS 577
            I + AQN  +   GAFTGE S   LKDIG +++++GHSERR    E D+ I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  +  +    VGGASLKA +
Sbjct:   59 IMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_SHIBS (Triosephosphate isomerase OS=Shigella boydii serotype 4 (strain Sb227) GN=tpiA PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 9.667e-39
Identity = 91/184 (49.46%), Postives = 115/184 (62.50%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQS 577
            I + AQN  +   GAFTGE S   LKDIG +++++GHSERR    E D+ I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  +  +    VGGASLKA +
Sbjct:   59 IMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_SHIB3 (Triosephosphate isomerase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=tpiA PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 9.667e-39
Identity = 91/184 (49.46%), Postives = 115/184 (62.50%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAY--ADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQS 577
            I + AQN  +   GAFTGE S   LKDIG +++++GHSERR    E D+ I KK A    +GL  + CIGE   E EAGKT +VC +Q+ A        +++  VIAYEPVWAIGTGK ATP QAQ VH  +RD +   V A++A +  I YGGSVN SN AEL  +  +    VGGASLKA +
Sbjct:   59 IMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIA-KVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKADA 241
The following BLAST results are available for this feature:
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIS_FLAJ14.339e-3944.62Triosephosphate isomerase OS=Flavobacterium johnso... [more]
TPIS_PASMU5.668e-3950.55Triosephosphate isomerase OS=Pasteurella multocida... [more]
TPIS_HAEIN5.668e-3949.45Triosephosphate isomerase OS=Haemophilus influenza... [more]
TPIS_HAEIE5.668e-3949.45Triosephosphate isomerase OS=Haemophilus influenza... [more]
TPIS_SHISS9.667e-3949.46Triosephosphate isomerase OS=Shigella sonnei (stra... [more]
TPIS_SHIFL9.667e-3949.46Triosephosphate isomerase OS=Shigella flexneri GN=... [more]
TPIS_SHIF89.667e-3949.46Triosephosphate isomerase OS=Shigella flexneri ser... [more]
TPIS_SHIDS9.667e-3949.46Triosephosphate isomerase OS=Shigella dysenteriae ... [more]
TPIS_SHIBS9.667e-3949.46Triosephosphate isomerase OS=Shigella boydii serot... [more]
TPIS_SHIB39.667e-3949.46Triosephosphate isomerase OS=Shigella boydii serot... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00919 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00919 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623839 ID=BQ623839; Name=BQ623839; organism=Citrus sinensis; type=EST; length=612bp
TGATCAAGTGAAGAATTCATTAACAGATAGAATTGAGATAGCAGCTCAGA
ACTCTTGGGTCGGAAAAGGTGGAGCATTTACAGGAGAAATCAGTGTGGAA
CAACTGAAAGATATTGGCTGCAAGTGGGTTGTTCTGGGGCATTCTGAGCG
CAGACATGTTATTGGTGAAGATGATCAGTTTATAGGGAAGAAAGCTGCCT
ATGCCTTGAGCGAGGGACTTGGTGTAATAGCTTGTATCGGGGAACAGTTG
CAAGAGAGAGAAGCAGGAAAAACTTTTGATGTCTGTTTTCAGCAACTTAA
AGCCTATGCAGATGCTATACCCAGTTGGGATAATGTAGTCATTGCATATG
AGCCTGTATGGGCTATTGGAACTGGTAAAGTGGCCACCCCCGAGCAGGCT
CAGGAAGTACATGCTGCTCTTCGTGATTGGCTTAAAAACAATGTCTCAGC
AGACGTTGCCACTAAAACACGTATTATCTATGGAGGGTCAGTAAATGGAA
GCAATTGTGCTGAACTTGCAAACAAGAGGATATTGATGGTTTTCTGGGTT
GGTGGCGCTTCCTTGAAGGCCCAGAGTTTGCTACCATTTGGCAATTCCCG
TAACATCCAAGA
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