BQ623839

Overview
NameBQ623839
Unique NameBQ623839
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length612
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_CLOBK (Triosephosphate isomerase OS=Clostridium botulinum (strain Okra / Type B1) GN=tpiA PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 1.952e-31
Identity = 84/201 (41.79%), Postives = 114/201 (56.72%), Query Frame = 2 
Query:    2 DQVKNSLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADAI--PSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVN-GSNCAELANKRILMVFWVGGASLKAQSLLPFGN 595
            ++VK S    I++ AQN    + GA+TGEI+ + L+++G  +V++GHSERR    E D+ + KK   A    L  I C GE L+ERE   T  V   Q+K     +     + +VIAYEP+WAIGTGK AT EQA E    +R  +K+     VA K RI YGGSV  G+  A++A + I     VGGASLKA+      N
Sbjct:   51 EEVKGS---NIKVGAQNMHFEESGAYTGEIAPKMLEELGVHYVIIGHSERRQYFNETDETVNKKVKKAFEHNLIPIVCCGESLEEREGNITEKVLEGQIKVGLKELSKEQVEKLVIAYEPIWAIGTGKTATDEQANETIGYIRTVVKDMYGESVADKVRIQYGGSVKPGTIKAQMAKEEIDGAL-VGGASLKAKDFAAIVN 247
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_LACSS (Triosephosphate isomerase OS=Lactobacillus sakei subsp. sakei (strain 23K) GN=tpiA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.550e-31
Identity = 79/189 (41.80%), Postives = 109/189 (57.67%), Query Frame = 2 
Query:   32 IEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKA-----YADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLL 583
            +++AA+N +  + GAFTGE S + L ++G  +V++GHSERR    E D+ I KKA       +  I C GE L+ REAGK  +    Q+        AD + S   +VIAYEP+WAIGTGK A+ +QAQE+ A +R  +       VA K RI YGGSV  +N  E+  K  +    VGGAS++  S L
Sbjct:   59 LKVAAENCYFEEAGAFTGETSPKTLNEMGVDYVIIGHSERRDYFHETDEDINKKAHAIFKNNMTPIFCCGESLETREAGKAEEWVSNQVTEGLKGLSADQVSS---MVIAYEPIWAIGTGKTASADQAQEICAVVRQTVAKLYDQTVADKVRIQYGGSVKPANVKEIMGKEDIDGGLVGGASMEPASFL 244
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_FRATT (Triosephosphate isomerase OS=Francisella tularensis subsp. tularensis GN=tpiA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.550e-31
Identity = 77/202 (38.12%), Postives = 112/202 (55.45%), Query Frame = 2 
Query:    5 QVKNSLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYAD--AIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLLPFGNSRN 604
            +V + L +++ +  QN      GA+TGEIS   L+DIGC ++++GHSERR +  E D+ + KK    +   +  + CIGE L +R++GK   V   QL    +  ++     VVIAYEPVWAIGTG VA+ EQ QE H  +R  L   V   +A   +I+YGGS+   N  ++ +   +    +GGASLKA       N  N
Sbjct:   48 EVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIGCDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRQSGKLKQVLATQLSLILENLSVEQLAKVVIAYEPVWAIGTGVVASLEQIQETHQFIRSLLA-KVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIINQAN 248
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_FRATM (Triosephosphate isomerase OS=Francisella tularensis subsp. mediasiatica (strain FSC147) GN=tpiA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.550e-31
Identity = 77/202 (38.12%), Postives = 112/202 (55.45%), Query Frame = 2 
Query:    5 QVKNSLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYAD--AIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLLPFGNSRN 604
            +V + L +++ +  QN      GA+TGEIS   L+DIGC ++++GHSERR +  E D+ + KK    +   +  + CIGE L +R++GK   V   QL    +  ++     VVIAYEPVWAIGTG VA+ EQ QE H  +R  L   V   +A   +I+YGGS+   N  ++ +   +    +GGASLKA       N  N
Sbjct:   48 EVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIGCDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRQSGKLKQVLATQLSLILENLSVEQLAKVVIAYEPVWAIGTGVVASLEQIQETHQFIRSLLA-KVDEKLAKNIKIVYGGSLKAENAKDILSLPNVDGGLIGGASLKAVEFNEIINQAN 248
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_FRAT1 (Triosephosphate isomerase OS=Francisella tularensis subsp. tularensis (strain FSC 198) GN=tpiA PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.550e-31
Identity = 77/202 (38.12%), Postives = 112/202 (55.45%), Query Frame = 2 
Query:    5 QVKNSLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYAD--AIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLLPFGNSRN 604
            +V + L +++ +  QN      GA+TGEIS   L+DIGC ++++GHSERR +  E D+ + KK    +   +  + CIGE L +R++GK   V   QL    +  ++     VVIAYEPVWAIGTG VA+ EQ QE H  +R  L   V   +A   +I+YGGS+   N  ++ +   +    +GGASLKA       N  N
Sbjct:   48 EVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIGCDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRQSGKLKQVLATQLSLILENLSVEQLAKVVIAYEPVWAIGTGVVASLEQIQETHQFIRSLLA-KVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIINQAN 248
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_XANAC (Triosephosphate isomerase OS=Xanthomonas axonopodis pv. citri GN=tpiA PE=3 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 3.330e-31
Identity = 79/196 (40.31%), Postives = 111/196 (56.63%), Query Frame = 2 
Query:    2 DQVKNSLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADAIPS--WDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLL 583
            D +++     +   AQ+    + GA+TGE+S   L D+G  + ++GHSERR    E  + + +K A AL  GL  + C+GE L++REAG+T  +   QL      + S  +   V+AYEP+WAIGTG+ ATPEQAQ VHA LR  +     A +A    I+YGGSV   N  EL  +  +    VGGASL A+  L
Sbjct:   48 DLIEDFEAHHLSFGAQDVSSNEKGAYTGEVSASMLVDVGAGYGLVGHSERRQYHQESSELVARKFAAALHAGLIPVLCVGESLEQREAGQTEAILRAQLDPVLALVGSAGFAGAVLAYEPIWAIGTGRTATPEQAQAVHAFLRGEVA-KADARIADSLPILYGGSVKPDNAGELFAQPDVDGGLVGGASLVAEDFL 242
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_RHOPB (Triosephosphate isomerase OS=Rhodopseudomonas palustris (strain BisB18) GN=tpiA PE=3 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 3.330e-31
Identity = 81/189 (42.86%), Postives = 104/189 (55.03%), Query Frame = 2 
Query:   17 SLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYADAIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLL 583
            S ++   I AQ+      GA TG+IS E L D G   V++GHSERR   GE D  + +KA  A   GL  I C+GE   +R+AG+T DV   QL        +  N V+AYEPVWAIGTG+  T    ++VH  +R  L   +S +  +K RI+YGGSV  SN  EL     +    VGGASLKA   L
Sbjct:   63 SRSNSFAIGAQDCHPRASGAHTGDISAEMLADAGASAVIVGHSERRADHGESDALVREKAEAAWRAGLVAIICVGETQGQRDAGQTLDVVGTQLAGSLPDGATAANTVVAYEPVWAIGTGRTPTVADVEQVHGFIRKSLTQRMSGE-GSKVRILYGGSVKPSNAQELMAVADVNGALVGGASLKAGDFL 250
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_FRATO (Triosephosphate isomerase OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=tpiA PE=3 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 3.330e-31
Identity = 77/202 (38.12%), Postives = 112/202 (55.45%), Query Frame = 2 
Query:    5 QVKNSLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYAD--AIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLLPFGNSRN 604
            +V + L +++ +  QN      GA+TGEIS   L+DIGC ++++GHSERR +  E D+ + KK    +   +  + CIGE L +R++GK   V   QL    +  ++     VVIAYEPVWAIGTG VA+ EQ QE H  +R  L   V   +A   +I+YGGS+   N  ++ +   +    +GGASLKA       N  N
Sbjct:   48 EVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIGCDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRKSGKLKQVLATQLSLILENLSVEQLAKVVIAYEPVWAIGTGVVASLEQIQETHQFIRSLLA-KVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIINQAN 248
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_FRATH (Triosephosphate isomerase OS=Francisella tularensis subsp. holarctica (strain LVS) GN=tpiA PE=3 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 3.330e-31
Identity = 77/202 (38.12%), Postives = 112/202 (55.45%), Query Frame = 2 
Query:    5 QVKNSLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYAD--AIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLLPFGNSRN 604
            +V + L +++ +  QN      GA+TGEIS   L+DIGC ++++GHSERR +  E D+ + KK    +   +  + CIGE L +R++GK   V   QL    +  ++     VVIAYEPVWAIGTG VA+ EQ QE H  +R  L   V   +A   +I+YGGS+   N  ++ +   +    +GGASLKA       N  N
Sbjct:   48 EVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIGCDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRKSGKLKQVLATQLSLILENLSVEQLAKVVIAYEPVWAIGTGVVASLEQIQETHQFIRSLLA-KVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIINQAN 248
[Close]
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Match: TPIS_FRATF (Triosephosphate isomerase OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=tpiA PE=3 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 3.330e-31
Identity = 77/202 (38.12%), Postives = 112/202 (55.45%), Query Frame = 2 
Query:    5 QVKNSLTDRIEIAAQNSWVGKGGAFTGEISVEQLKDIGCKWVVLGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGEQLQEREAGKTFDVCFQQLKAYAD--AIPSWDNVVIAYEPVWAIGTGKVATPEQAQEVHAALRDWLKNNVSADVATKTRIIYGGSVNGSNCAELANKRILMVFWVGGASLKAQSLLPFGNSRN 604
            +V + L +++ +  QN      GA+TGEIS   L+DIGC ++++GHSERR +  E D+ + KK    +   +  + CIGE L +R++GK   V   QL    +  ++     VVIAYEPVWAIGTG VA+ EQ QE H  +R  L   V   +A   +I+YGGS+   N  ++ +   +    +GGASLKA       N  N
Sbjct:   48 EVISQLPEKVGVGLQNITFYDDGAYTGEISARMLEDIGCDYLLIGHSERRSLFAESDEDVFKKLNKIIDTTITPVVCIGESLDDRKSGKLKQVLATQLSLILENLSVEQLAKVVIAYEPVWAIGTGVVASLEQIQETHQFIRSLLA-KVDERLAKNIKIVYGGSLKAENAKDILSLPDVDGGLIGGASLKAAEFNEIINQAN 248
The following BLAST results are available for this feature:
BLAST of BQ623839 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIS_CLOBK1.952e-3141.79Triosephosphate isomerase OS=Clostridium botulinum... [more]
TPIS_LACSS2.550e-3141.80Triosephosphate isomerase OS=Lactobacillus sakei s... [more]
TPIS_FRATT2.550e-3138.12Triosephosphate isomerase OS=Francisella tularensi... [more]
TPIS_FRATM2.550e-3138.12Triosephosphate isomerase OS=Francisella tularensi... [more]
TPIS_FRAT12.550e-3138.12Triosephosphate isomerase OS=Francisella tularensi... [more]
TPIS_XANAC3.330e-3140.31Triosephosphate isomerase OS=Xanthomonas axonopodi... [more]
TPIS_RHOPB3.330e-3142.86Triosephosphate isomerase OS=Rhodopseudomonas palu... [more]
TPIS_FRATO3.330e-3138.12Triosephosphate isomerase OS=Francisella tularensi... [more]
TPIS_FRATH3.330e-3138.12Triosephosphate isomerase OS=Francisella tularensi... [more]
TPIS_FRATF3.330e-3138.12Triosephosphate isomerase OS=Francisella tularensi... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00919 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00919 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623839 ID=BQ623839; Name=BQ623839; organism=Citrus sinensis; type=EST; length=612bp
TGATCAAGTGAAGAATTCATTAACAGATAGAATTGAGATAGCAGCTCAGA
ACTCTTGGGTCGGAAAAGGTGGAGCATTTACAGGAGAAATCAGTGTGGAA
CAACTGAAAGATATTGGCTGCAAGTGGGTTGTTCTGGGGCATTCTGAGCG
CAGACATGTTATTGGTGAAGATGATCAGTTTATAGGGAAGAAAGCTGCCT
ATGCCTTGAGCGAGGGACTTGGTGTAATAGCTTGTATCGGGGAACAGTTG
CAAGAGAGAGAAGCAGGAAAAACTTTTGATGTCTGTTTTCAGCAACTTAA
AGCCTATGCAGATGCTATACCCAGTTGGGATAATGTAGTCATTGCATATG
AGCCTGTATGGGCTATTGGAACTGGTAAAGTGGCCACCCCCGAGCAGGCT
CAGGAAGTACATGCTGCTCTTCGTGATTGGCTTAAAAACAATGTCTCAGC
AGACGTTGCCACTAAAACACGTATTATCTATGGAGGGTCAGTAAATGGAA
GCAATTGTGCTGAACTTGCAAACAAGAGGATATTGATGGTTTTCTGGGTT
GGTGGCGCTTCCTTGAAGGCCCAGAGTTTGCTACCATTTGGCAATTCCCG
TAACATCCAAGA
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