BQ623952 | Citrus Genome Database

Overview


Name	BQ623952
Unique Name	BQ623952
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	668

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENO_CHLRE (Enolase (Fragment) OS=Chlamydomonas reinhardtii GN=ENO PE=2 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 2.027e-70
Identity = 71/95 (74.74%), Postives = 81/95 (85.26%), Query Frame = 2

Query:   77 NIDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            ++DGT N     K KLGANAILAVS+AVCKAGA  K +PLYKHIA+L+GN  L+LPVP+FN+INGGSHAGN LAMQEFMILPVGASSF EAM+MG
Sbjct:   33 DLDGTDN-----KGKLGANAILAVSMAVCKAGAAEKGVPLYKHIADLAGNSKLILPVPSFNIINGGSHAGNALAMQEFMILPVGASSFSEAMRMG 122

HSP 2 Score: 136.732 bits (343), Expect = 2.027e-70
Identity = 67/99 (67.68%), Postives = 76/99 (76.77%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSF 662
            EVYH LK +IK KYGQDA NVGDEGGFAPNI  N EGL L+N AI KAGYTGKV IGMDVA+SEFY  D  YDL+FK + NDGSQK + + + +LY  F
Sbjct:  124 EVYHALKGLIKAKYGQDACNVGDEGGFAPNIGSNDEGLNLVNEAIEKAGYTGKVKIGMDVASSEFYTEDGMYDLDFKNQPNDGSQKKTKEQMLELYNEF 222

HSP 3 Score: 30.8018 bits (68), Expect = 2.027e-70
Identity = 14/23 (60.87%), Postives = 15/23 (65.22%), Query Frame = 1

Query:    1 NAIIGPALAGKDPTEQTAIDNYM 69
            NAII PAL G DP +Q  ID  M
Sbjct:    9 NAIIAPALKGMDPVKQAEIDQKM 31

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENO_LOLPE (Enolase OS=Loligo pealeii PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 7.574e-70
Identity = 71/94 (75.53%), Postives = 79/94 (84.04%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K KLGANAIL VSLAVCKAGA  K +PLY+HIA+L+GNK ++LPVPAFNVINGGSHAGNKLAMQEFMILP GA +F EAMKMG
Sbjct:   97 LDGTEN-----KNKLGANAILGVSLAVCKAGAAAKGVPLYRHIADLAGNKEVILPVPAFNVINGGSHAGNKLAMQEFMILPTGAKNFTEAMKMG 185

HSP 2 Score: 137.117 bits (344), Expect = 7.574e-70
Identity = 67/100 (67.00%), Postives = 81/100 (81.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYHHLK+VIKKKYGQDA NVGDEGGFAPNI +NKEGLELL TAIA AGYT ++ IGMDVAASEF   +K YDL+FK  +++ +  ++ D L D+YK F+
Sbjct:  187 EVYHHLKSVIKKKYGQDACNVGDEGGFAPNILDNKEGLELLKTAIANAGYTAEIEIGMDVAASEFC-KEKKYDLDFKNPDSNPNDWLTSDQLADVYKDFV 285

HSP 3 Score: 27.7202 bits (60), Expect = 7.574e-70
Identity = 13/26 (50.00%), Postives = 18/26 (69.23%), Query Frame = 1

Query:    1 NAIIGPALAGK--DPTEQTAIDNYMV 72
            N II PAL  K  D  +QTA+DN+++
Sbjct:   70 NKIIAPALVDKKMDEKDQTAVDNFLL 95

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENOA_PONAB (Alpha-enolase OS=Pongo abelii GN=ENO1 PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 3.072e-68
Identity = 77/100 (77.00%), Postives = 84/100 (84.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYH+LK VIK+KYG+DATNVGDEGGFAPNI ENKEGLELL TAI KAGYT KVVIGMDVAASEF+ S K YDL+FK   +D S+ IS D L DLYKSFI
Sbjct:  187 EVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGK-YDLDFKSP-DDPSRYISPDQLADLYKSFI 284

HSP 2 Score: 137.502 bits (345), Expect = 3.072e-68
Identity = 68/94 (72.34%), Postives = 80/94 (85.11%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K K GANAIL VSLAVCKAGA  K +PLY+HIA+L+GN  ++LPVPAFNVINGGSHAGNKLAMQEFMILPVGA++F+EAM++G
Sbjct:   97 MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIG 185

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENOA_MACFA (Alpha-enolase OS=Macaca fascicularis GN=ENO1 PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 3.072e-68
Identity = 77/100 (77.00%), Postives = 84/100 (84.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYH+LK VIK+KYG+DATNVGDEGGFAPNI ENKEGLELL TAI KAGYT KVVIGMDVAASEF+ S K YDL+FK   +D S+ IS D L DLYKSFI
Sbjct:  187 EVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGK-YDLDFKSP-DDPSRYISPDQLADLYKSFI 284

HSP 2 Score: 137.502 bits (345), Expect = 3.072e-68
Identity = 68/94 (72.34%), Postives = 80/94 (85.11%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K K GANAIL VSLAVCKAGA  K +PLY+HIA+L+GN  ++LPVPAFNVINGGSHAGNKLAMQEFMILPVGA++F+EAM++G
Sbjct:   97 MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIG 185

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENOA_HUMAN (Alpha-enolase OS=Homo sapiens GN=ENO1 PE=1 SV=2)

HSP 1 Score: 142.895 bits (359), Expect = 3.072e-68
Identity = 77/100 (77.00%), Postives = 84/100 (84.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYH+LK VIK+KYG+DATNVGDEGGFAPNI ENKEGLELL TAI KAGYT KVVIGMDVAASEF+ S K YDL+FK   +D S+ IS D L DLYKSFI
Sbjct:  187 EVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGK-YDLDFKSP-DDPSRYISPDQLADLYKSFI 284

HSP 2 Score: 137.502 bits (345), Expect = 3.072e-68
Identity = 68/94 (72.34%), Postives = 80/94 (85.11%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K K GANAIL VSLAVCKAGA  K +PLY+HIA+L+GN  ++LPVPAFNVINGGSHAGNKLAMQEFMILPVGA++F+EAM++G
Sbjct:   97 MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIG 185

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENOA_MOUSE (Alpha-enolase OS=Mus musculus GN=Eno1 PE=1 SV=3)

HSP 1 Score: 140.584 bits (353), Expect = 4.006e-68
Identity = 75/100 (75.00%), Postives = 84/100 (84.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYH+LK VIK+KYG+DATNVGDEGGFAPNI ENKE LELL TAIAKAGYT +VVIGMDVAASEFY S K YDL+FK   +D S+ I+ D L DLYKSF+
Sbjct:  187 EVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGK-YDLDFKSP-DDPSRYITPDQLADLYKSFV 284

HSP 2 Score: 139.428 bits (350), Expect = 4.006e-68
Identity = 70/94 (74.47%), Postives = 80/94 (85.11%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K K GANAIL VSLAVCKAGA  K +PLY+HIA+L+GN  ++LPVPAFNVINGGSHAGNKLAMQEFMILPVGASSF+EAM++G
Sbjct:   97 MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIG 185

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENOB_RAT (Beta-enolase OS=Rattus norvegicus GN=Eno3 PE=1 SV=3)

HSP 1 Score: 143.28 bits (360), Expect = 8.883e-68
Identity = 73/94 (77.66%), Postives = 81/94 (86.17%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K K GANAIL VSLAVCKAGA  K +PLY+HIA+L+GN +LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAM++G
Sbjct:   97 LDGTEN-----KSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIG 185

HSP 2 Score: 135.576 bits (340), Expect = 8.883e-68
Identity = 72/100 (72.00%), Postives = 80/100 (80.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYHHLK VIK KYG+DATNVGDEGGFAPNI EN E LELL TAI  AGY  KVVIGMDVAASEFY + K YDL+FK   +D ++ ISG+ L +LYKSFI
Sbjct:  187 EVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGK-YDLDFKSP-DDPARHISGEKLGELYKSFI 284

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENOA_RAT (Alpha-enolase OS=Rattus norvegicus GN=Eno1 PE=1 SV=4)

HSP 1 Score: 139.428 bits (350), Expect = 1.158e-67
Identity = 70/94 (74.47%), Postives = 80/94 (85.11%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K K GANAIL VSLAVCKAGA  K +PLY+HIA+L+GN  ++LPVPAFNVINGGSHAGNKLAMQEFMILPVGASSF+EAM++G
Sbjct:   97 MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIG 185

HSP 2 Score: 139.043 bits (349), Expect = 1.158e-67
Identity = 74/100 (74.00%), Postives = 84/100 (84.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYH+LK VIK+KYG+DATNVGDEGGFAPNI ENKE LELL +AIAKAGYT +VVIGMDVAASEFY + K YDL+FK   +D S+ I+ D L DLYKSFI
Sbjct:  187 EVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKSAIAKAGYTDQVVIGMDVAASEFYRAGK-YDLDFKSP-DDASRYITPDQLADLYKSFI 284

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENOB_MOUSE (Beta-enolase OS=Mus musculus GN=Eno3 PE=1 SV=3)

HSP 1 Score: 143.28 bits (360), Expect = 1.970e-67
Identity = 73/94 (77.66%), Postives = 81/94 (86.17%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K K GANAIL VSLAVCKAGA  K +PLY+HIA+L+GN +LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAM++G
Sbjct:   97 LDGTEN-----KSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIG 185

HSP 2 Score: 134.42 bits (337), Expect = 1.970e-67
Identity = 71/100 (71.00%), Postives = 80/100 (80.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYHHLK VIK KYG+DATNVGDEGGFAPNI EN E LELL TAI  AGY  KVVIGMDVAASEFY + K YDL+FK   +D ++ ISG+ L +LYK+FI
Sbjct:  187 EVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGK-YDLDFKSP-DDPARHISGEKLGELYKNFI 284

[Close]

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Match: ENOB_HUMAN (Beta-enolase OS=Homo sapiens GN=ENO3 PE=1 SV=4)

HSP 1 Score: 142.895 bits (359), Expect = 2.569e-67
Identity = 72/94 (76.60%), Postives = 81/94 (86.17%), Query Frame = 2

Query:   80 IDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMG 361
            +DGT N     K K GANAIL VSLAVCKAGA  K +PLY+HIA+L+GN +L+LPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAM++G
Sbjct:   97 LDGTEN-----KSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIG 185

HSP 2 Score: 134.42 bits (337), Expect = 2.569e-67
Identity = 71/100 (71.00%), Postives = 80/100 (80.00%), Query Frame = 3

Query:  366 EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLNTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFI 665
            EVYHHLK VIK KYG+DATNVGDEGGFAPNI EN E LELL TAI  AGY  KVVIGMDVAASEFY + K YDL+FK   +D ++ I+G+ L +LYKSFI
Sbjct:  187 EVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGK-YDLDFKSP-DDPARHITGEKLGELYKSFI 284

The following BLAST results are available for this feature:

BLAST of BQ623952 vs. ExPASy Swiss-Prot

BLAST of BQ623952 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
ENO_CHLRE	2.027e-70	74.74	Enolase (Fragment) OS=Chlamydomonas reinhardtii GN...	[more]
ENO_LOLPE	7.574e-70	75.53	Enolase OS=Loligo pealeii PE=2 SV=1	[more]
ENOA_PONAB	3.072e-68	77.00	Alpha-enolase OS=Pongo abelii GN=ENO1 PE=2 SV=1	[more]
ENOA_MACFA	3.072e-68	77.00	Alpha-enolase OS=Macaca fascicularis GN=ENO1 PE=2 ...	[more]
ENOA_HUMAN	3.072e-68	77.00	Alpha-enolase OS=Homo sapiens GN=ENO1 PE=1 SV=2	[more]
ENOA_MOUSE	4.006e-68	75.00	Alpha-enolase OS=Mus musculus GN=Eno1 PE=1 SV=3	[more]
ENOB_RAT	8.883e-68	77.66	Beta-enolase OS=Rattus norvegicus GN=Eno3 PE=1 SV=...	[more]
ENOA_RAT	1.158e-67	74.47	Alpha-enolase OS=Rattus norvegicus GN=Eno1 PE=1 SV...	[more]
ENOB_MOUSE	1.970e-67	77.66	Beta-enolase OS=Mus musculus GN=Eno3 PE=1 SV=3	[more]
ENOB_HUMAN	2.569e-67	76.60	Beta-enolase OS=Homo sapiens GN=ENO3 PE=1 SV=4	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_01043 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_01043 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623952 ID=BQ623952; Name=BQ623952; organism=Citrus sinensis; type=EST; length=668bp
AACGCCATCATTGGCCCTGCACTCGCTGGCAAGGACCCAACTGAGCAGAC
TGCTATTGACAACTATATGGTACAACAACATTGATGGAACTGTAAATGAG
TGGGGTTGGTGCAAACAGAAGCTTGGAGCAAACGCCATTTTGGCTGTCTC
CCTAGCTGTCTGCAAGGCTGGTGCACATGTCAAGAAGATTCCTCTTTACA
AGCATATTGCCGAACTCTCTGGTAACAAGAATTTGGTGTTGCCAGTGCCA
GCATTCAATGTCATTAATGGTGGATCACATGCTGGAAACAAACTTGCTAT
GCAGGAGTTTATGATTCTTCCTGTAGGAGCTTCCTCTTTCAAAGAAGCCA
TGAAGATGGGTGGTAGAAGTATATCACCATCTGAAGGCTGTGATTAAAAA
GAAATACGGCCAGGATGCTACAAATGTTGGTGATGAAGGCGGTTTTGCTC
CCAATATTCAGGAGAACAAGGAAGGACTTGAATTGCTCAACACAGCCATT
GCTAAAGCCGGTTACACAGGCAAAGTTGTCATTGGAATGGATGTTGCTGC
ATCTGAATTCTATGGCTCAGATAAGACTTATGATCTGAACTTCAAGGAAG
AAAACAATGATGGTTCACAAAAGATCTCAGGAGATGCACTCAAAGATCTC
TATAAGTCATTCATCTCT