BQ624227

Overview
NameBQ624227
Unique NameBQ624227
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length578
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: MIP_CANFA (Lens fiber major intrinsic protein OS=Canis familiaris GN=MIP PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 5.792e-14
Identity = 19/38 (50.00%), Postives = 29/38 (76.32%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHI 379
            PA SF PA+++  +TNHWVYW+GP IG  + +++YD +
Sbjct:  185 PARSFAPAILTRNFTNHWVYWVGPIIGGGLGSLLYDFL 222

HSP 2 Score: 43.8986 bits (102), Expect = 5.792e-14
Identity = 25/69 (36.23%), Postives = 37/69 (53.62%), Query Frame = 1 
Query:   73 HLAVSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            H  VS   A   EI +T   V  ++AT  + + G LG++A +A+GF +    L G  + GA MNP+  F
Sbjct:  122 HPGVSVGQATTVEIFLTLQFVLCIFATYDERRNGRLGSVA-LAVGFSLTLGHLFGMYYTGAGMNPARSF 189
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP51_MAIZE (Aquaporin TIP5-1 OS=Zea mays GN=TIP5-1 PE=2 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 1.270e-13
Identity = 23/35 (65.71%), Postives = 27/35 (77.14%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVY 370
            PA SFGPAVVS  + N  VYW GP +GAA+AA+VY
Sbjct:  209 PARSFGPAVVSGHFKNQAVYWAGPMVGAAVAALVY 243

HSP 2 Score: 45.4394 bits (106), Expect = 1.270e-13
Identity = 27/77 (35.06%), Postives = 39/77 (50.65%), Query Frame = 1 
Query:   76 LAVSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLG---------TIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            +A++ +   V E V+TF LVYTV+       +   G          +  +A+G   GA +LA GA  GASMNP+  F
Sbjct:  137 VAMTGFGGAVLEGVLTFLLVYTVHVVGEREPRSRGGDGKREFAATALGALAVGLTQGAFVLAAGALTGASMNPARSF 213
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: MIP_RAT (Lens fiber major intrinsic protein (Fragment) OS=Rattus norvegicus GN=Mip PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 1.650e-13
Identity = 18/38 (47.37%), Postives = 29/38 (76.32%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHI 379
            PA SF PA+++  ++NHWVYW+GP IG  + +++YD +
Sbjct:  183 PARSFAPAILTRNFSNHWVYWVGPIIGGGLGSLLYDFL 220

HSP 2 Score: 43.8986 bits (102), Expect = 1.650e-13
Identity = 24/69 (34.78%), Postives = 37/69 (53.62%), Query Frame = 1 
Query:   73 HLAVSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            H  VS   A   EI +T   V  ++AT  + + G +G++A +A+GF +    L G  + GA MNP+  F
Sbjct:  120 HAGVSVGQATTVEIFLTLQFVLCIFATYDERRNGRMGSVA-LAVGFSLTLGHLFGMYYTGAGMNPARSF 187
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: MIP_MOUSE (Lens fiber major intrinsic protein OS=Mus musculus GN=Mip PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 1.650e-13
Identity = 18/38 (47.37%), Postives = 29/38 (76.32%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHI 379
            PA SF PA+++  ++NHWVYW+GP IG  + +++YD +
Sbjct:  185 PARSFAPAILTRNFSNHWVYWVGPIIGGGLGSLLYDFL 222

HSP 2 Score: 43.8986 bits (102), Expect = 1.650e-13
Identity = 24/69 (34.78%), Postives = 37/69 (53.62%), Query Frame = 1 
Query:   73 HLAVSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            H  VS   A   EI +T   V  ++AT  + + G +G++A +A+GF +    L G  + GA MNP+  F
Sbjct:  122 HAGVSVGQATTVEIFLTLQFVLCIFATYDERRNGRMGSVA-LAVGFSLTLGHLFGMYYTGAGMNPARSF 189
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: AQP1_PONAB (Aquaporin-1 OS=Pongo abelii GN=AQP1 PE=2 SV=3)

HSP 1 Score: 57.7658 bits (138), Expect = 2.782e-13
Identity = 21/38 (55.26%), Postives = 31/38 (81.58%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHI 379
            PA SFG AV++  ++NHW++W+GPFIG A+A ++YD I
Sbjct:  193 PARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFI 230

HSP 2 Score: 37.7354 bits (86), Expect = 2.782e-13
Identity = 25/68 (36.76%), Postives = 35/68 (51.47%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLFWS 285
            V+S   +  EI+ T  LV  V AT  D ++ +LG  AP+AIG  V    L    + G  +NP+  F S
Sbjct:  133 VNSGQGLGIEIIGTLQLVLCVLATT-DRRRRDLGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGS 199
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: AQP1_HUMAN (Aquaporin-1 OS=Homo sapiens GN=AQP1 PE=1 SV=3)

HSP 1 Score: 57.7658 bits (138), Expect = 2.782e-13
Identity = 21/38 (55.26%), Postives = 31/38 (81.58%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHI 379
            PA SFG AV++  ++NHW++W+GPFIG A+A ++YD I
Sbjct:  193 PARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFI 230

HSP 2 Score: 37.7354 bits (86), Expect = 2.782e-13
Identity = 25/68 (36.76%), Postives = 35/68 (51.47%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLFWS 285
            V+S   +  EI+ T  LV  V AT  D ++ +LG  AP+AIG  V    L    + G  +NP+  F S
Sbjct:  133 VNSGQGLGIEIIGTLQLVLCVLATT-DRRRRDLGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGS 199
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: AQP8_RAT (Aquaporin-8 OS=Rattus norvegicus GN=Aqp8 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 2.784e-13
Identity = 30/61 (49.18%), Postives = 37/61 (60.66%), Query Frame = 1 
Query:   97 AVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            A+  EIVMT  LV  V   AV+ K   +G +AP +IGF V  +ILAGG   GA MNP+  F
Sbjct:  159 ALGVEIVMTMLLVLAVCMGAVNEK--TMGPLAPFSIGFSVIVDILAGGGISGACMNPARAF 217

HSP 2 Score: 45.4394 bits (106), Expect = 2.784e-13
Identity = 18/42 (42.86%), Postives = 26/42 (61.90%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDD 391
            PA +FGPAV++  W  HW+YWLGP +      ++   +FI D
Sbjct:  213 PARAFGPAVMAGYWDFHWIYWLGPLLAGLFVGLLI-RLFIGD 253
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: AQP1_PIG (Aquaporin-1 OS=Sus scrofa GN=AQP1 PE=2 SV=3)

HSP 1 Score: 55.4546 bits (132), Expect = 3.612e-13
Identity = 21/38 (55.26%), Postives = 30/38 (78.95%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHI 379
            PA SFG AV++  + +HWV+W+GPFIG A+A ++YD I
Sbjct:  195 PARSFGSAVITHNFQDHWVFWVGPFIGGALAVLIYDFI 232

HSP 2 Score: 39.6614 bits (91), Expect = 3.612e-13
Identity = 26/68 (38.24%), Postives = 35/68 (51.47%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLFWS 285
            V S   +  EI+ T  LV  V AT  D ++ +LG  AP+AIGF V    L    + G  +NP+  F S
Sbjct:  135 VDSGQGLGIEIIGTLQLVLCVLATT-DRRRRDLGGSAPLAIGFSVALGHLLAIDYTGCGINPARSFGS 201
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: AQP8_NOTAL (Aquaporin-8 OS=Notomys alexis GN=AQP8 PE=2 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 3.616e-13
Identity = 28/61 (45.90%), Postives = 37/61 (60.66%), Query Frame = 1 
Query:   97 AVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            A+  EI++T  LV  V   AV+ K   +G +AP +IGF V  +ILAGG   GA MNP+  F
Sbjct:  157 ALGVEIILTILLVLAVCMGAVNEK--TMGPLAPFSIGFSVIVDILAGGGISGACMNPARAF 215

HSP 2 Score: 46.595 bits (109), Expect = 3.616e-13
Identity = 19/42 (45.24%), Postives = 26/42 (61.90%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDD 391
            PA +FGPAVV+  W  HW+YWLGP +      ++   +FI D
Sbjct:  211 PARAFGPAVVAGYWDFHWIYWLGPLLAGLFVGLLI-RLFIGD 251
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: AQP_AEDAE (Aquaporin AQPAe.a OS=Aedes aegypti GN=AAEL003512 PE=2 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 3.622e-13
Identity = 22/39 (56.41%), Postives = 26/39 (66.67%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIF 382
            PA SFGPAVV   WT+ WVYW+GP +G  +A  VY   F
Sbjct:  199 PARSFGPAVVMGNWTDQWVYWVGPIVGGILAGAVYRLFF 237

HSP 2 Score: 40.817 bits (94), Expect = 3.622e-13
Identity = 22/66 (33.33%), Postives = 39/66 (59.09%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            +++   V+ E ++TF LV+ V+    D ++ ++   AP+AIG  + A  L+   + GASMNP+  F
Sbjct:  139 LTAGQGVMIEALITFILVFVVHGVC-DNRRSDIKGSAPLAIGLSITAGHLSAIKYTGASMNPARSF 203
The following BLAST results are available for this feature:
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 75
Match NameE-valueIdentityDescription
MIP_CANFA5.792e-1450.00Lens fiber major intrinsic protein OS=Canis famili... [more]
TIP51_MAIZE1.270e-1365.71Aquaporin TIP5-1 OS=Zea mays GN=TIP5-1 PE=2 SV=1[more]
MIP_RAT1.650e-1347.37Lens fiber major intrinsic protein (Fragment) OS=R... [more]
MIP_MOUSE1.650e-1347.37Lens fiber major intrinsic protein OS=Mus musculus... [more]
AQP1_PONAB2.782e-1355.26Aquaporin-1 OS=Pongo abelii GN=AQP1 PE=2 SV=3[more]
AQP1_HUMAN2.782e-1355.26Aquaporin-1 OS=Homo sapiens GN=AQP1 PE=1 SV=3[more]
AQP8_RAT2.784e-1349.18Aquaporin-8 OS=Rattus norvegicus GN=Aqp8 PE=2 SV=1[more]
AQP1_PIG3.612e-1355.26Aquaporin-1 OS=Sus scrofa GN=AQP1 PE=2 SV=3[more]
AQP8_NOTAL3.616e-1345.90Aquaporin-8 OS=Notomys alexis GN=AQP8 PE=2 SV=1[more]
AQP_AEDAE3.622e-1356.41Aquaporin AQPAe.a OS=Aedes aegypti GN=AAEL003512 P... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUSDA-FP_01318 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_01318 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ624227 ID=BQ624227; Name=BQ624227; organism=Citrus sinensis; type=EST; length=578bp
TAGGATCAGTTGTTGCTTGCTTGCTGCTCAAGTTTTCCACCGGTGGATTG
GAAACATCGGCATTCGCACTATCATCTGGCCGTCTCATCATGGAATGCAG
TTGTGTTTGAGATTGTGATGACCTTTGGCTTGGTCTACACAGTGTATGCA
ACAGCAGTGGATCCAAAGAAGGGAAATCTGGGCACTATTGCACCAATTGC
AATCGGTTTCATTGTTGGTGCCAACATTTTGGCTGGTGGCGCTTTTGATG
GTGCATCAATGAACCCCAGCAGTCTCTTTTGGTCCTGCTGTGGTTAGCTG
GACTTGGACTAACCACTGGGTCTACTGGCTTGGACCATTTATTGGTGCCG
CGATTGCGGCCATTGTTTACGACCACATCTTCATCGATGACAACGCTCAC
CAGCCACTTCCTGCCAATGATTTCTAAGAATCTAATTAATCATAAACCTT
TGTCTCTCTTATCGTTTTAGATCGTGGAGAGAATTGAAGCTGTGTTTCTT
TGTACTTTTATTGTTGGTGCTGTTGTTATCATTTATATTAGAAGGAGTGG
ATGGATTTATGTCTTGCTAAAAAAAAAA
back to top